Job ID = 6626498 SRX = SRX5736477 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 25234765 spots for SRR8956888/SRR8956888.sra Written 25234765 spots for SRR8956888/SRR8956888.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626779 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:02:37 25234765 reads; of these: 25234765 (100.00%) were paired; of these: 4341603 (17.20%) aligned concordantly 0 times 13990043 (55.44%) aligned concordantly exactly 1 time 6903119 (27.36%) aligned concordantly >1 times ---- 4341603 pairs aligned concordantly 0 times; of these: 806225 (18.57%) aligned discordantly 1 time ---- 3535378 pairs aligned 0 times concordantly or discordantly; of these: 7070756 mates make up the pairs; of these: 5470959 (77.37%) aligned 0 times 705924 (9.98%) aligned exactly 1 time 893873 (12.64%) aligned >1 times 89.16% overall alignment rate Time searching: 01:02:37 Overall time: 01:02:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 2204371 / 21586165 = 0.1021 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:24:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:24:27: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:24:27: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:24:34: 1000000 INFO @ Tue, 14 Jul 2020 08:24:41: 2000000 INFO @ Tue, 14 Jul 2020 08:24:49: 3000000 INFO @ Tue, 14 Jul 2020 08:24:55: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:24:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:24:57: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:24:57: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:25:03: 5000000 INFO @ Tue, 14 Jul 2020 08:25:05: 1000000 INFO @ Tue, 14 Jul 2020 08:25:10: 6000000 INFO @ Tue, 14 Jul 2020 08:25:12: 2000000 INFO @ Tue, 14 Jul 2020 08:25:17: 7000000 INFO @ Tue, 14 Jul 2020 08:25:20: 3000000 INFO @ Tue, 14 Jul 2020 08:25:25: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:25:27: 4000000 INFO @ Tue, 14 Jul 2020 08:25:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:25:27: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:25:27: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:25:32: 9000000 INFO @ Tue, 14 Jul 2020 08:25:35: 1000000 INFO @ Tue, 14 Jul 2020 08:25:35: 5000000 INFO @ Tue, 14 Jul 2020 08:25:40: 10000000 INFO @ Tue, 14 Jul 2020 08:25:42: 2000000 INFO @ Tue, 14 Jul 2020 08:25:43: 6000000 INFO @ Tue, 14 Jul 2020 08:25:47: 11000000 INFO @ Tue, 14 Jul 2020 08:25:49: 3000000 INFO @ Tue, 14 Jul 2020 08:25:51: 7000000 INFO @ Tue, 14 Jul 2020 08:25:55: 12000000 INFO @ Tue, 14 Jul 2020 08:25:57: 4000000 INFO @ Tue, 14 Jul 2020 08:25:59: 8000000 INFO @ Tue, 14 Jul 2020 08:26:02: 13000000 INFO @ Tue, 14 Jul 2020 08:26:04: 5000000 INFO @ Tue, 14 Jul 2020 08:26:06: 9000000 INFO @ Tue, 14 Jul 2020 08:26:10: 14000000 INFO @ Tue, 14 Jul 2020 08:26:11: 6000000 INFO @ Tue, 14 Jul 2020 08:26:14: 10000000 INFO @ Tue, 14 Jul 2020 08:26:17: 15000000 INFO @ Tue, 14 Jul 2020 08:26:18: 7000000 INFO @ Tue, 14 Jul 2020 08:26:22: 11000000 INFO @ Tue, 14 Jul 2020 08:26:24: 16000000 INFO @ Tue, 14 Jul 2020 08:26:25: 8000000 INFO @ Tue, 14 Jul 2020 08:26:30: 12000000 INFO @ Tue, 14 Jul 2020 08:26:32: 17000000 INFO @ Tue, 14 Jul 2020 08:26:32: 9000000 INFO @ Tue, 14 Jul 2020 08:26:37: 13000000 INFO @ Tue, 14 Jul 2020 08:26:39: 18000000 INFO @ Tue, 14 Jul 2020 08:26:40: 10000000 INFO @ Tue, 14 Jul 2020 08:26:45: 14000000 INFO @ Tue, 14 Jul 2020 08:26:46: 19000000 INFO @ Tue, 14 Jul 2020 08:26:47: 11000000 INFO @ Tue, 14 Jul 2020 08:26:52: 15000000 INFO @ Tue, 14 Jul 2020 08:26:53: 20000000 INFO @ Tue, 14 Jul 2020 08:26:54: 12000000 INFO @ Tue, 14 Jul 2020 08:27:00: 16000000 INFO @ Tue, 14 Jul 2020 08:27:01: 21000000 INFO @ Tue, 14 Jul 2020 08:27:01: 13000000 INFO @ Tue, 14 Jul 2020 08:27:07: 17000000 INFO @ Tue, 14 Jul 2020 08:27:08: 22000000 INFO @ Tue, 14 Jul 2020 08:27:08: 14000000 INFO @ Tue, 14 Jul 2020 08:27:14: 18000000 INFO @ Tue, 14 Jul 2020 08:27:15: 15000000 INFO @ Tue, 14 Jul 2020 08:27:15: 23000000 INFO @ Tue, 14 Jul 2020 08:27:21: 19000000 INFO @ Tue, 14 Jul 2020 08:27:22: 16000000 INFO @ Tue, 14 Jul 2020 08:27:22: 24000000 INFO @ Tue, 14 Jul 2020 08:27:28: 20000000 INFO @ Tue, 14 Jul 2020 08:27:29: 17000000 INFO @ Tue, 14 Jul 2020 08:27:29: 25000000 INFO @ Tue, 14 Jul 2020 08:27:35: 21000000 INFO @ Tue, 14 Jul 2020 08:27:36: 18000000 INFO @ Tue, 14 Jul 2020 08:27:36: 26000000 INFO @ Tue, 14 Jul 2020 08:27:42: 22000000 INFO @ Tue, 14 Jul 2020 08:27:43: 19000000 INFO @ Tue, 14 Jul 2020 08:27:44: 27000000 INFO @ Tue, 14 Jul 2020 08:27:49: 23000000 INFO @ Tue, 14 Jul 2020 08:27:50: 20000000 INFO @ Tue, 14 Jul 2020 08:27:51: 28000000 INFO @ Tue, 14 Jul 2020 08:27:57: 24000000 INFO @ Tue, 14 Jul 2020 08:27:57: 21000000 INFO @ Tue, 14 Jul 2020 08:27:58: 29000000 INFO @ Tue, 14 Jul 2020 08:28:04: 22000000 INFO @ Tue, 14 Jul 2020 08:28:04: 25000000 INFO @ Tue, 14 Jul 2020 08:28:05: 30000000 INFO @ Tue, 14 Jul 2020 08:28:11: 23000000 INFO @ Tue, 14 Jul 2020 08:28:11: 26000000 INFO @ Tue, 14 Jul 2020 08:28:13: 31000000 INFO @ Tue, 14 Jul 2020 08:28:17: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:28:19: 27000000 INFO @ Tue, 14 Jul 2020 08:28:20: 32000000 INFO @ Tue, 14 Jul 2020 08:28:24: 25000000 INFO @ Tue, 14 Jul 2020 08:28:26: 28000000 INFO @ Tue, 14 Jul 2020 08:28:27: 33000000 INFO @ Tue, 14 Jul 2020 08:28:31: 26000000 INFO @ Tue, 14 Jul 2020 08:28:33: 29000000 INFO @ Tue, 14 Jul 2020 08:28:34: 34000000 INFO @ Tue, 14 Jul 2020 08:28:38: 27000000 INFO @ Tue, 14 Jul 2020 08:28:41: 30000000 INFO @ Tue, 14 Jul 2020 08:28:41: 35000000 INFO @ Tue, 14 Jul 2020 08:28:45: 28000000 INFO @ Tue, 14 Jul 2020 08:28:48: 36000000 INFO @ Tue, 14 Jul 2020 08:28:48: 31000000 INFO @ Tue, 14 Jul 2020 08:28:51: 29000000 INFO @ Tue, 14 Jul 2020 08:28:55: 37000000 INFO @ Tue, 14 Jul 2020 08:28:55: 32000000 INFO @ Tue, 14 Jul 2020 08:28:58: 30000000 INFO @ Tue, 14 Jul 2020 08:29:02: 38000000 INFO @ Tue, 14 Jul 2020 08:29:02: 33000000 INFO @ Tue, 14 Jul 2020 08:29:05: 31000000 INFO @ Tue, 14 Jul 2020 08:29:09: 39000000 INFO @ Tue, 14 Jul 2020 08:29:10: 34000000 INFO @ Tue, 14 Jul 2020 08:29:12: 32000000 INFO @ Tue, 14 Jul 2020 08:29:16: 40000000 INFO @ Tue, 14 Jul 2020 08:29:17: 35000000 INFO @ Tue, 14 Jul 2020 08:29:18: 33000000 INFO @ Tue, 14 Jul 2020 08:29:20: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:29:20: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:29:20: #1 total tags in treatment: 18746926 INFO @ Tue, 14 Jul 2020 08:29:20: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:29:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:29:21: #1 tags after filtering in treatment: 16426784 INFO @ Tue, 14 Jul 2020 08:29:21: #1 Redundant rate of treatment: 0.12 INFO @ Tue, 14 Jul 2020 08:29:21: #1 finished! INFO @ Tue, 14 Jul 2020 08:29:21: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:29:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:29:22: #2 number of paired peaks: 46 WARNING @ Tue, 14 Jul 2020 08:29:22: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:29:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:29:24: 36000000 INFO @ Tue, 14 Jul 2020 08:29:25: 34000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:29:31: 37000000 INFO @ Tue, 14 Jul 2020 08:29:32: 35000000 INFO @ Tue, 14 Jul 2020 08:29:38: 38000000 INFO @ Tue, 14 Jul 2020 08:29:39: 36000000 INFO @ Tue, 14 Jul 2020 08:29:46: 39000000 INFO @ Tue, 14 Jul 2020 08:29:46: 37000000 INFO @ Tue, 14 Jul 2020 08:29:53: 40000000 INFO @ Tue, 14 Jul 2020 08:29:53: 38000000 INFO @ Tue, 14 Jul 2020 08:29:57: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:29:57: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:29:57: #1 total tags in treatment: 18746926 INFO @ Tue, 14 Jul 2020 08:29:57: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:29:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:29:57: #1 tags after filtering in treatment: 16426784 INFO @ Tue, 14 Jul 2020 08:29:57: #1 Redundant rate of treatment: 0.12 INFO @ Tue, 14 Jul 2020 08:29:57: #1 finished! INFO @ Tue, 14 Jul 2020 08:29:57: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:29:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:29:58: #2 number of paired peaks: 46 WARNING @ Tue, 14 Jul 2020 08:29:58: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:29:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:30:00: 39000000 INFO @ Tue, 14 Jul 2020 08:30:07: 40000000 INFO @ Tue, 14 Jul 2020 08:30:11: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:30:11: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:30:11: #1 total tags in treatment: 18746926 INFO @ Tue, 14 Jul 2020 08:30:11: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:30:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:30:11: #1 tags after filtering in treatment: 16426784 INFO @ Tue, 14 Jul 2020 08:30:11: #1 Redundant rate of treatment: 0.12 INFO @ Tue, 14 Jul 2020 08:30:11: #1 finished! INFO @ Tue, 14 Jul 2020 08:30:11: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:30:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:30:12: #2 number of paired peaks: 46 WARNING @ Tue, 14 Jul 2020 08:30:12: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:30:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736477/SRX5736477.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling