Job ID = 6626486 SRX = SRX5736467 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 9332430 spots for SRR8956879/SRR8956879.sra Written 9332430 spots for SRR8956879/SRR8956879.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626681 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:25:37 9332430 reads; of these: 9332430 (100.00%) were paired; of these: 1677233 (17.97%) aligned concordantly 0 times 5830295 (62.47%) aligned concordantly exactly 1 time 1824902 (19.55%) aligned concordantly >1 times ---- 1677233 pairs aligned concordantly 0 times; of these: 195741 (11.67%) aligned discordantly 1 time ---- 1481492 pairs aligned 0 times concordantly or discordantly; of these: 2962984 mates make up the pairs; of these: 2281281 (76.99%) aligned 0 times 261879 (8.84%) aligned exactly 1 time 419824 (14.17%) aligned >1 times 87.78% overall alignment rate Time searching: 00:25:37 Overall time: 00:25:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 733003 / 7822758 = 0.0937 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:39:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:39:24: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:39:24: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:39:30: 1000000 INFO @ Tue, 14 Jul 2020 07:39:37: 2000000 INFO @ Tue, 14 Jul 2020 07:39:43: 3000000 INFO @ Tue, 14 Jul 2020 07:39:49: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:39:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:39:54: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:39:54: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:39:55: 5000000 INFO @ Tue, 14 Jul 2020 07:40:01: 1000000 INFO @ Tue, 14 Jul 2020 07:40:03: 6000000 INFO @ Tue, 14 Jul 2020 07:40:09: 2000000 INFO @ Tue, 14 Jul 2020 07:40:10: 7000000 INFO @ Tue, 14 Jul 2020 07:40:17: 3000000 INFO @ Tue, 14 Jul 2020 07:40:17: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:40:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:40:24: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:40:24: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:40:24: 4000000 INFO @ Tue, 14 Jul 2020 07:40:25: 9000000 INFO @ Tue, 14 Jul 2020 07:40:32: 1000000 INFO @ Tue, 14 Jul 2020 07:40:32: 5000000 INFO @ Tue, 14 Jul 2020 07:40:32: 10000000 INFO @ Tue, 14 Jul 2020 07:40:39: 11000000 INFO @ Tue, 14 Jul 2020 07:40:39: 6000000 INFO @ Tue, 14 Jul 2020 07:40:39: 2000000 INFO @ Tue, 14 Jul 2020 07:40:46: 12000000 INFO @ Tue, 14 Jul 2020 07:40:47: 7000000 INFO @ Tue, 14 Jul 2020 07:40:47: 3000000 INFO @ Tue, 14 Jul 2020 07:40:53: 8000000 INFO @ Tue, 14 Jul 2020 07:40:53: 13000000 INFO @ Tue, 14 Jul 2020 07:40:54: 4000000 INFO @ Tue, 14 Jul 2020 07:41:01: 14000000 INFO @ Tue, 14 Jul 2020 07:41:01: 9000000 INFO @ Tue, 14 Jul 2020 07:41:02: 5000000 INFO @ Tue, 14 Jul 2020 07:41:07: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 07:41:07: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 07:41:07: #1 total tags in treatment: 6928826 INFO @ Tue, 14 Jul 2020 07:41:07: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:41:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:41:07: #1 tags after filtering in treatment: 6489487 INFO @ Tue, 14 Jul 2020 07:41:07: #1 Redundant rate of treatment: 0.06 INFO @ Tue, 14 Jul 2020 07:41:07: #1 finished! INFO @ Tue, 14 Jul 2020 07:41:07: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:41:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:41:08: #2 number of paired peaks: 43 WARNING @ Tue, 14 Jul 2020 07:41:08: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:41:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:41:08: 10000000 INFO @ Tue, 14 Jul 2020 07:41:09: 6000000 INFO @ Tue, 14 Jul 2020 07:41:15: 11000000 INFO @ Tue, 14 Jul 2020 07:41:17: 7000000 INFO @ Tue, 14 Jul 2020 07:41:23: 12000000 INFO @ Tue, 14 Jul 2020 07:41:24: 8000000 INFO @ Tue, 14 Jul 2020 07:41:30: 13000000 INFO @ Tue, 14 Jul 2020 07:41:32: 9000000 INFO @ Tue, 14 Jul 2020 07:41:37: 14000000 INFO @ Tue, 14 Jul 2020 07:41:39: 10000000 INFO @ Tue, 14 Jul 2020 07:41:44: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 07:41:44: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 07:41:44: #1 total tags in treatment: 6928826 INFO @ Tue, 14 Jul 2020 07:41:44: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:41:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:41:44: #1 tags after filtering in treatment: 6489487 INFO @ Tue, 14 Jul 2020 07:41:44: #1 Redundant rate of treatment: 0.06 INFO @ Tue, 14 Jul 2020 07:41:44: #1 finished! INFO @ Tue, 14 Jul 2020 07:41:44: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:41:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:41:44: #2 number of paired peaks: 43 WARNING @ Tue, 14 Jul 2020 07:41:44: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:41:44: Process for pairing-model is terminated! BedGraph に変換しました。 BigWig に変換中... cut: /home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:41:47: 11000000 INFO @ Tue, 14 Jul 2020 07:41:54: 12000000 INFO @ Tue, 14 Jul 2020 07:42:01: 13000000 INFO @ Tue, 14 Jul 2020 07:42:08: 14000000 INFO @ Tue, 14 Jul 2020 07:42:14: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 07:42:14: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 07:42:14: #1 total tags in treatment: 6928826 INFO @ Tue, 14 Jul 2020 07:42:14: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:42:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:42:14: #1 tags after filtering in treatment: 6489487 INFO @ Tue, 14 Jul 2020 07:42:14: #1 Redundant rate of treatment: 0.06 INFO @ Tue, 14 Jul 2020 07:42:14: #1 finished! INFO @ Tue, 14 Jul 2020 07:42:14: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:42:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:42:15: #2 number of paired peaks: 43 WARNING @ Tue, 14 Jul 2020 07:42:15: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:42:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736467/SRX5736467.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。