Job ID = 6626485 SRX = SRX5736466 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 23333128 spots for SRR8956878/SRR8956878.sra Written 23333128 spots for SRR8956878/SRR8956878.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626961 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:19:17 23333128 reads; of these: 23333128 (100.00%) were paired; of these: 3935196 (16.87%) aligned concordantly 0 times 10318800 (44.22%) aligned concordantly exactly 1 time 9079132 (38.91%) aligned concordantly >1 times ---- 3935196 pairs aligned concordantly 0 times; of these: 152737 (3.88%) aligned discordantly 1 time ---- 3782459 pairs aligned 0 times concordantly or discordantly; of these: 7564918 mates make up the pairs; of these: 6171351 (81.58%) aligned 0 times 449232 (5.94%) aligned exactly 1 time 944335 (12.48%) aligned >1 times 86.78% overall alignment rate Time searching: 01:19:17 Overall time: 01:19:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1376029 / 19469449 = 0.0707 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:38:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:38:03: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:38:03: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:38:11: 1000000 INFO @ Tue, 14 Jul 2020 08:38:17: 2000000 INFO @ Tue, 14 Jul 2020 08:38:22: 3000000 INFO @ Tue, 14 Jul 2020 08:38:28: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:38:33: 5000000 INFO @ Tue, 14 Jul 2020 08:38:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:38:33: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:38:33: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:38:39: 6000000 INFO @ Tue, 14 Jul 2020 08:38:41: 1000000 INFO @ Tue, 14 Jul 2020 08:38:48: 7000000 INFO @ Tue, 14 Jul 2020 08:38:48: 2000000 INFO @ Tue, 14 Jul 2020 08:38:56: 3000000 INFO @ Tue, 14 Jul 2020 08:38:56: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:39:02: 9000000 INFO @ Tue, 14 Jul 2020 08:39:03: 4000000 INFO @ Tue, 14 Jul 2020 08:39:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:39:03: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:39:03: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:39:09: 10000000 INFO @ Tue, 14 Jul 2020 08:39:10: 5000000 INFO @ Tue, 14 Jul 2020 08:39:10: 1000000 INFO @ Tue, 14 Jul 2020 08:39:15: 11000000 INFO @ Tue, 14 Jul 2020 08:39:17: 6000000 INFO @ Tue, 14 Jul 2020 08:39:18: 2000000 INFO @ Tue, 14 Jul 2020 08:39:22: 12000000 INFO @ Tue, 14 Jul 2020 08:39:25: 7000000 INFO @ Tue, 14 Jul 2020 08:39:26: 3000000 INFO @ Tue, 14 Jul 2020 08:39:28: 13000000 INFO @ Tue, 14 Jul 2020 08:39:32: 8000000 INFO @ Tue, 14 Jul 2020 08:39:33: 4000000 INFO @ Tue, 14 Jul 2020 08:39:34: 14000000 INFO @ Tue, 14 Jul 2020 08:39:39: 9000000 INFO @ Tue, 14 Jul 2020 08:39:41: 5000000 INFO @ Tue, 14 Jul 2020 08:39:41: 15000000 INFO @ Tue, 14 Jul 2020 08:39:47: 10000000 INFO @ Tue, 14 Jul 2020 08:39:47: 16000000 INFO @ Tue, 14 Jul 2020 08:39:48: 6000000 INFO @ Tue, 14 Jul 2020 08:39:53: 17000000 INFO @ Tue, 14 Jul 2020 08:39:54: 11000000 INFO @ Tue, 14 Jul 2020 08:39:56: 7000000 INFO @ Tue, 14 Jul 2020 08:40:00: 18000000 INFO @ Tue, 14 Jul 2020 08:40:01: 12000000 INFO @ Tue, 14 Jul 2020 08:40:03: 8000000 INFO @ Tue, 14 Jul 2020 08:40:06: 19000000 INFO @ Tue, 14 Jul 2020 08:40:09: 13000000 INFO @ Tue, 14 Jul 2020 08:40:11: 9000000 INFO @ Tue, 14 Jul 2020 08:40:13: 20000000 INFO @ Tue, 14 Jul 2020 08:40:16: 14000000 INFO @ Tue, 14 Jul 2020 08:40:18: 10000000 INFO @ Tue, 14 Jul 2020 08:40:19: 21000000 INFO @ Tue, 14 Jul 2020 08:40:23: 15000000 INFO @ Tue, 14 Jul 2020 08:40:26: 11000000 INFO @ Tue, 14 Jul 2020 08:40:26: 22000000 INFO @ Tue, 14 Jul 2020 08:40:30: 16000000 INFO @ Tue, 14 Jul 2020 08:40:33: 23000000 INFO @ Tue, 14 Jul 2020 08:40:34: 12000000 INFO @ Tue, 14 Jul 2020 08:40:38: 17000000 INFO @ Tue, 14 Jul 2020 08:40:40: 24000000 INFO @ Tue, 14 Jul 2020 08:40:41: 13000000 INFO @ Tue, 14 Jul 2020 08:40:45: 18000000 INFO @ Tue, 14 Jul 2020 08:40:47: 25000000 INFO @ Tue, 14 Jul 2020 08:40:49: 14000000 INFO @ Tue, 14 Jul 2020 08:40:52: 19000000 INFO @ Tue, 14 Jul 2020 08:40:54: 26000000 INFO @ Tue, 14 Jul 2020 08:40:56: 15000000 INFO @ Tue, 14 Jul 2020 08:41:00: 27000000 INFO @ Tue, 14 Jul 2020 08:41:00: 20000000 INFO @ Tue, 14 Jul 2020 08:41:04: 16000000 INFO @ Tue, 14 Jul 2020 08:41:06: 28000000 INFO @ Tue, 14 Jul 2020 08:41:08: 21000000 INFO @ Tue, 14 Jul 2020 08:41:11: 17000000 INFO @ Tue, 14 Jul 2020 08:41:15: 29000000 INFO @ Tue, 14 Jul 2020 08:41:15: 22000000 INFO @ Tue, 14 Jul 2020 08:41:19: 18000000 INFO @ Tue, 14 Jul 2020 08:41:21: 30000000 INFO @ Tue, 14 Jul 2020 08:41:23: 23000000 INFO @ Tue, 14 Jul 2020 08:41:27: 19000000 INFO @ Tue, 14 Jul 2020 08:41:27: 31000000 INFO @ Tue, 14 Jul 2020 08:41:31: 24000000 INFO @ Tue, 14 Jul 2020 08:41:34: 32000000 INFO @ Tue, 14 Jul 2020 08:41:34: 20000000 INFO @ Tue, 14 Jul 2020 08:41:39: 25000000 INFO @ Tue, 14 Jul 2020 08:41:40: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:41:42: 21000000 INFO @ Tue, 14 Jul 2020 08:41:47: 26000000 INFO @ Tue, 14 Jul 2020 08:41:47: 34000000 INFO @ Tue, 14 Jul 2020 08:41:49: 22000000 INFO @ Tue, 14 Jul 2020 08:41:53: 35000000 INFO @ Tue, 14 Jul 2020 08:41:54: 27000000 INFO @ Tue, 14 Jul 2020 08:41:57: 23000000 INFO @ Tue, 14 Jul 2020 08:42:00: 36000000 INFO @ Tue, 14 Jul 2020 08:42:02: 28000000 INFO @ Tue, 14 Jul 2020 08:42:04: 24000000 INFO @ Tue, 14 Jul 2020 08:42:06: 37000000 INFO @ Tue, 14 Jul 2020 08:42:10: 29000000 INFO @ Tue, 14 Jul 2020 08:42:11: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:42:11: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:42:11: #1 total tags in treatment: 18023185 INFO @ Tue, 14 Jul 2020 08:42:11: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:42:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:42:11: #1 tags after filtering in treatment: 15857492 INFO @ Tue, 14 Jul 2020 08:42:11: #1 Redundant rate of treatment: 0.12 INFO @ Tue, 14 Jul 2020 08:42:11: #1 finished! INFO @ Tue, 14 Jul 2020 08:42:11: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:42:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:42:12: 25000000 INFO @ Tue, 14 Jul 2020 08:42:12: #2 number of paired peaks: 80 WARNING @ Tue, 14 Jul 2020 08:42:12: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:42:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:42:18: 30000000 INFO @ Tue, 14 Jul 2020 08:42:20: 26000000 INFO @ Tue, 14 Jul 2020 08:42:26: 31000000 INFO @ Tue, 14 Jul 2020 08:42:27: 27000000 INFO @ Tue, 14 Jul 2020 08:42:33: 32000000 INFO @ Tue, 14 Jul 2020 08:42:35: 28000000 INFO @ Tue, 14 Jul 2020 08:42:41: 33000000 INFO @ Tue, 14 Jul 2020 08:42:42: 29000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:42:49: 34000000 INFO @ Tue, 14 Jul 2020 08:42:50: 30000000 INFO @ Tue, 14 Jul 2020 08:42:56: 35000000 INFO @ Tue, 14 Jul 2020 08:42:57: 31000000 INFO @ Tue, 14 Jul 2020 08:43:04: 36000000 INFO @ Tue, 14 Jul 2020 08:43:05: 32000000 INFO @ Tue, 14 Jul 2020 08:43:11: 37000000 INFO @ Tue, 14 Jul 2020 08:43:13: 33000000 INFO @ Tue, 14 Jul 2020 08:43:16: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:43:16: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:43:16: #1 total tags in treatment: 18023185 INFO @ Tue, 14 Jul 2020 08:43:16: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:43:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:43:17: #1 tags after filtering in treatment: 15857492 INFO @ Tue, 14 Jul 2020 08:43:17: #1 Redundant rate of treatment: 0.12 INFO @ Tue, 14 Jul 2020 08:43:17: #1 finished! INFO @ Tue, 14 Jul 2020 08:43:17: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:43:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:43:18: #2 number of paired peaks: 80 WARNING @ Tue, 14 Jul 2020 08:43:18: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:43:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:43:20: 34000000 INFO @ Tue, 14 Jul 2020 08:43:27: 35000000 INFO @ Tue, 14 Jul 2020 08:43:34: 36000000 INFO @ Tue, 14 Jul 2020 08:43:41: 37000000 INFO @ Tue, 14 Jul 2020 08:43:46: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:43:46: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:43:46: #1 total tags in treatment: 18023185 INFO @ Tue, 14 Jul 2020 08:43:46: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:43:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:43:47: #1 tags after filtering in treatment: 15857492 INFO @ Tue, 14 Jul 2020 08:43:47: #1 Redundant rate of treatment: 0.12 INFO @ Tue, 14 Jul 2020 08:43:47: #1 finished! INFO @ Tue, 14 Jul 2020 08:43:47: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:43:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:43:48: #2 number of paired peaks: 80 WARNING @ Tue, 14 Jul 2020 08:43:48: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:43:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736466/SRX5736466.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling