Job ID = 6626484 SRX = SRX5736465 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 26375590 spots for SRR8956877/SRR8956877.sra Written 26375590 spots for SRR8956877/SRR8956877.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626924 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:16:37 26375590 reads; of these: 26375590 (100.00%) were paired; of these: 3867907 (14.66%) aligned concordantly 0 times 13058442 (49.51%) aligned concordantly exactly 1 time 9449241 (35.83%) aligned concordantly >1 times ---- 3867907 pairs aligned concordantly 0 times; of these: 655165 (16.94%) aligned discordantly 1 time ---- 3212742 pairs aligned 0 times concordantly or discordantly; of these: 6425484 mates make up the pairs; of these: 4578891 (71.26%) aligned 0 times 570738 (8.88%) aligned exactly 1 time 1275855 (19.86%) aligned >1 times 91.32% overall alignment rate Time searching: 01:16:37 Overall time: 01:16:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1861257 / 23022010 = 0.0808 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:39:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:39:10: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:39:10: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:39:15: 1000000 INFO @ Tue, 14 Jul 2020 08:39:20: 2000000 INFO @ Tue, 14 Jul 2020 08:39:25: 3000000 INFO @ Tue, 14 Jul 2020 08:39:29: 4000000 INFO @ Tue, 14 Jul 2020 08:39:34: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:39:39: 6000000 INFO @ Tue, 14 Jul 2020 08:39:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:39:40: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:39:40: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:39:44: 7000000 INFO @ Tue, 14 Jul 2020 08:39:45: 1000000 INFO @ Tue, 14 Jul 2020 08:39:49: 8000000 INFO @ Tue, 14 Jul 2020 08:39:49: 2000000 INFO @ Tue, 14 Jul 2020 08:39:54: 9000000 INFO @ Tue, 14 Jul 2020 08:39:54: 3000000 INFO @ Tue, 14 Jul 2020 08:39:59: 10000000 INFO @ Tue, 14 Jul 2020 08:39:59: 4000000 INFO @ Tue, 14 Jul 2020 08:40:03: 11000000 INFO @ Tue, 14 Jul 2020 08:40:04: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:40:08: 12000000 INFO @ Tue, 14 Jul 2020 08:40:09: 6000000 INFO @ Tue, 14 Jul 2020 08:40:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:40:10: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:40:10: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:40:13: 13000000 INFO @ Tue, 14 Jul 2020 08:40:14: 7000000 INFO @ Tue, 14 Jul 2020 08:40:17: 1000000 INFO @ Tue, 14 Jul 2020 08:40:18: 14000000 INFO @ Tue, 14 Jul 2020 08:40:19: 8000000 INFO @ Tue, 14 Jul 2020 08:40:23: 15000000 INFO @ Tue, 14 Jul 2020 08:40:23: 9000000 INFO @ Tue, 14 Jul 2020 08:40:23: 2000000 INFO @ Tue, 14 Jul 2020 08:40:28: 16000000 INFO @ Tue, 14 Jul 2020 08:40:28: 10000000 INFO @ Tue, 14 Jul 2020 08:40:29: 3000000 INFO @ Tue, 14 Jul 2020 08:40:33: 17000000 INFO @ Tue, 14 Jul 2020 08:40:33: 11000000 INFO @ Tue, 14 Jul 2020 08:40:35: 4000000 INFO @ Tue, 14 Jul 2020 08:40:38: 18000000 INFO @ Tue, 14 Jul 2020 08:40:38: 12000000 INFO @ Tue, 14 Jul 2020 08:40:41: 5000000 INFO @ Tue, 14 Jul 2020 08:40:43: 19000000 INFO @ Tue, 14 Jul 2020 08:40:43: 13000000 INFO @ Tue, 14 Jul 2020 08:40:47: 6000000 INFO @ Tue, 14 Jul 2020 08:40:48: 20000000 INFO @ Tue, 14 Jul 2020 08:40:48: 14000000 INFO @ Tue, 14 Jul 2020 08:40:53: 21000000 INFO @ Tue, 14 Jul 2020 08:40:53: 15000000 INFO @ Tue, 14 Jul 2020 08:40:53: 7000000 INFO @ Tue, 14 Jul 2020 08:40:58: 22000000 INFO @ Tue, 14 Jul 2020 08:40:58: 16000000 INFO @ Tue, 14 Jul 2020 08:40:59: 8000000 INFO @ Tue, 14 Jul 2020 08:41:02: 23000000 INFO @ Tue, 14 Jul 2020 08:41:03: 17000000 INFO @ Tue, 14 Jul 2020 08:41:05: 9000000 INFO @ Tue, 14 Jul 2020 08:41:07: 24000000 INFO @ Tue, 14 Jul 2020 08:41:07: 18000000 INFO @ Tue, 14 Jul 2020 08:41:11: 10000000 INFO @ Tue, 14 Jul 2020 08:41:12: 19000000 INFO @ Tue, 14 Jul 2020 08:41:12: 25000000 INFO @ Tue, 14 Jul 2020 08:41:17: 11000000 INFO @ Tue, 14 Jul 2020 08:41:17: 20000000 INFO @ Tue, 14 Jul 2020 08:41:17: 26000000 INFO @ Tue, 14 Jul 2020 08:41:22: 21000000 INFO @ Tue, 14 Jul 2020 08:41:22: 27000000 INFO @ Tue, 14 Jul 2020 08:41:23: 12000000 INFO @ Tue, 14 Jul 2020 08:41:27: 22000000 INFO @ Tue, 14 Jul 2020 08:41:27: 28000000 INFO @ Tue, 14 Jul 2020 08:41:29: 13000000 INFO @ Tue, 14 Jul 2020 08:41:32: 23000000 INFO @ Tue, 14 Jul 2020 08:41:32: 29000000 INFO @ Tue, 14 Jul 2020 08:41:35: 14000000 INFO @ Tue, 14 Jul 2020 08:41:37: 24000000 INFO @ Tue, 14 Jul 2020 08:41:37: 30000000 INFO @ Tue, 14 Jul 2020 08:41:41: 15000000 INFO @ Tue, 14 Jul 2020 08:41:42: 25000000 INFO @ Tue, 14 Jul 2020 08:41:42: 31000000 INFO @ Tue, 14 Jul 2020 08:41:46: 26000000 INFO @ Tue, 14 Jul 2020 08:41:47: 32000000 INFO @ Tue, 14 Jul 2020 08:41:47: 16000000 INFO @ Tue, 14 Jul 2020 08:41:51: 27000000 INFO @ Tue, 14 Jul 2020 08:41:52: 33000000 INFO @ Tue, 14 Jul 2020 08:41:53: 17000000 INFO @ Tue, 14 Jul 2020 08:41:56: 28000000 INFO @ Tue, 14 Jul 2020 08:41:57: 34000000 INFO @ Tue, 14 Jul 2020 08:41:59: 18000000 INFO @ Tue, 14 Jul 2020 08:42:01: 29000000 INFO @ Tue, 14 Jul 2020 08:42:01: 35000000 INFO @ Tue, 14 Jul 2020 08:42:05: 19000000 INFO @ Tue, 14 Jul 2020 08:42:06: 30000000 INFO @ Tue, 14 Jul 2020 08:42:06: 36000000 INFO @ Tue, 14 Jul 2020 08:42:11: 20000000 INFO @ Tue, 14 Jul 2020 08:42:11: 31000000 INFO @ Tue, 14 Jul 2020 08:42:11: 37000000 INFO @ Tue, 14 Jul 2020 08:42:16: 32000000 INFO @ Tue, 14 Jul 2020 08:42:16: 38000000 INFO @ Tue, 14 Jul 2020 08:42:17: 21000000 INFO @ Tue, 14 Jul 2020 08:42:21: 33000000 INFO @ Tue, 14 Jul 2020 08:42:21: 39000000 INFO @ Tue, 14 Jul 2020 08:42:22: 22000000 INFO @ Tue, 14 Jul 2020 08:42:25: 34000000 INFO @ Tue, 14 Jul 2020 08:42:26: 40000000 INFO @ Tue, 14 Jul 2020 08:42:28: 23000000 INFO @ Tue, 14 Jul 2020 08:42:30: 35000000 INFO @ Tue, 14 Jul 2020 08:42:31: 41000000 INFO @ Tue, 14 Jul 2020 08:42:34: 24000000 INFO @ Tue, 14 Jul 2020 08:42:35: 36000000 INFO @ Tue, 14 Jul 2020 08:42:36: 42000000 INFO @ Tue, 14 Jul 2020 08:42:40: 37000000 INFO @ Tue, 14 Jul 2020 08:42:40: 25000000 INFO @ Tue, 14 Jul 2020 08:42:41: 43000000 INFO @ Tue, 14 Jul 2020 08:42:45: 38000000 INFO @ Tue, 14 Jul 2020 08:42:46: 44000000 INFO @ Tue, 14 Jul 2020 08:42:46: 26000000 INFO @ Tue, 14 Jul 2020 08:42:48: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:42:48: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:42:48: #1 total tags in treatment: 20657011 INFO @ Tue, 14 Jul 2020 08:42:48: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:42:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:42:49: #1 tags after filtering in treatment: 17443414 INFO @ Tue, 14 Jul 2020 08:42:49: #1 Redundant rate of treatment: 0.16 INFO @ Tue, 14 Jul 2020 08:42:49: #1 finished! INFO @ Tue, 14 Jul 2020 08:42:49: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:42:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:42:50: #2 number of paired peaks: 69 WARNING @ Tue, 14 Jul 2020 08:42:50: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:42:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.05_peaks.narrowPeak: No such file or directory INFO @ Tue, 14 Jul 2020 08:42:50: 39000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:42:52: 27000000 INFO @ Tue, 14 Jul 2020 08:42:55: 40000000 INFO @ Tue, 14 Jul 2020 08:42:58: 28000000 INFO @ Tue, 14 Jul 2020 08:43:00: 41000000 INFO @ Tue, 14 Jul 2020 08:43:04: 29000000 INFO @ Tue, 14 Jul 2020 08:43:04: 42000000 INFO @ Tue, 14 Jul 2020 08:43:09: 43000000 INFO @ Tue, 14 Jul 2020 08:43:10: 30000000 INFO @ Tue, 14 Jul 2020 08:43:14: 44000000 INFO @ Tue, 14 Jul 2020 08:43:16: 31000000 INFO @ Tue, 14 Jul 2020 08:43:17: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:43:17: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:43:17: #1 total tags in treatment: 20657011 INFO @ Tue, 14 Jul 2020 08:43:17: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:43:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:43:17: #1 tags after filtering in treatment: 17443414 INFO @ Tue, 14 Jul 2020 08:43:17: #1 Redundant rate of treatment: 0.16 INFO @ Tue, 14 Jul 2020 08:43:17: #1 finished! INFO @ Tue, 14 Jul 2020 08:43:17: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:43:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:43:18: #2 number of paired peaks: 69 WARNING @ Tue, 14 Jul 2020 08:43:18: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:43:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:43:22: 32000000 INFO @ Tue, 14 Jul 2020 08:43:28: 33000000 INFO @ Tue, 14 Jul 2020 08:43:34: 34000000 INFO @ Tue, 14 Jul 2020 08:43:40: 35000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:43:46: 36000000 INFO @ Tue, 14 Jul 2020 08:43:52: 37000000 INFO @ Tue, 14 Jul 2020 08:43:57: 38000000 INFO @ Tue, 14 Jul 2020 08:44:03: 39000000 INFO @ Tue, 14 Jul 2020 08:44:09: 40000000 INFO @ Tue, 14 Jul 2020 08:44:15: 41000000 INFO @ Tue, 14 Jul 2020 08:44:21: 42000000 INFO @ Tue, 14 Jul 2020 08:44:27: 43000000 INFO @ Tue, 14 Jul 2020 08:44:33: 44000000 INFO @ Tue, 14 Jul 2020 08:44:36: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:44:36: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:44:36: #1 total tags in treatment: 20657011 INFO @ Tue, 14 Jul 2020 08:44:36: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:44:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:44:36: #1 tags after filtering in treatment: 17443414 INFO @ Tue, 14 Jul 2020 08:44:36: #1 Redundant rate of treatment: 0.16 INFO @ Tue, 14 Jul 2020 08:44:36: #1 finished! INFO @ Tue, 14 Jul 2020 08:44:36: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:44:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:44:37: #2 number of paired peaks: 69 WARNING @ Tue, 14 Jul 2020 08:44:37: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:44:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736465/SRX5736465.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。