Job ID = 6626483 SRX = SRX5736464 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 32467961 spots for SRR8956876/SRR8956876.sra Written 32467961 spots for SRR8956876/SRR8956876.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626981 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:23:39 32467961 reads; of these: 32467961 (100.00%) were paired; of these: 5730897 (17.65%) aligned concordantly 0 times 17131355 (52.76%) aligned concordantly exactly 1 time 9605709 (29.59%) aligned concordantly >1 times ---- 5730897 pairs aligned concordantly 0 times; of these: 1029892 (17.97%) aligned discordantly 1 time ---- 4701005 pairs aligned 0 times concordantly or discordantly; of these: 9402010 mates make up the pairs; of these: 7142360 (75.97%) aligned 0 times 760096 (8.08%) aligned exactly 1 time 1499554 (15.95%) aligned >1 times 89.00% overall alignment rate Time searching: 01:23:39 Overall time: 01:23:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1568259 / 27591988 = 0.0568 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:49:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:49:42: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:49:42: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:49:49: 1000000 INFO @ Tue, 14 Jul 2020 08:49:55: 2000000 INFO @ Tue, 14 Jul 2020 08:50:02: 3000000 INFO @ Tue, 14 Jul 2020 08:50:09: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:50:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:50:12: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:50:12: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:50:16: 5000000 INFO @ Tue, 14 Jul 2020 08:50:19: 1000000 INFO @ Tue, 14 Jul 2020 08:50:23: 6000000 INFO @ Tue, 14 Jul 2020 08:50:27: 2000000 INFO @ Tue, 14 Jul 2020 08:50:30: 7000000 INFO @ Tue, 14 Jul 2020 08:50:34: 3000000 INFO @ Tue, 14 Jul 2020 08:50:37: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:50:41: 4000000 INFO @ Tue, 14 Jul 2020 08:50:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:50:42: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:50:42: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:50:44: 9000000 INFO @ Tue, 14 Jul 2020 08:50:49: 5000000 INFO @ Tue, 14 Jul 2020 08:50:49: 1000000 INFO @ Tue, 14 Jul 2020 08:50:52: 10000000 INFO @ Tue, 14 Jul 2020 08:50:56: 6000000 INFO @ Tue, 14 Jul 2020 08:50:56: 2000000 INFO @ Tue, 14 Jul 2020 08:50:59: 11000000 INFO @ Tue, 14 Jul 2020 08:51:04: 7000000 INFO @ Tue, 14 Jul 2020 08:51:04: 3000000 INFO @ Tue, 14 Jul 2020 08:51:06: 12000000 INFO @ Tue, 14 Jul 2020 08:51:11: 4000000 INFO @ Tue, 14 Jul 2020 08:51:11: 8000000 INFO @ Tue, 14 Jul 2020 08:51:13: 13000000 INFO @ Tue, 14 Jul 2020 08:51:18: 5000000 INFO @ Tue, 14 Jul 2020 08:51:19: 9000000 INFO @ Tue, 14 Jul 2020 08:51:20: 14000000 INFO @ Tue, 14 Jul 2020 08:51:26: 6000000 INFO @ Tue, 14 Jul 2020 08:51:27: 10000000 INFO @ Tue, 14 Jul 2020 08:51:28: 15000000 INFO @ Tue, 14 Jul 2020 08:51:33: 7000000 INFO @ Tue, 14 Jul 2020 08:51:35: 11000000 INFO @ Tue, 14 Jul 2020 08:51:35: 16000000 INFO @ Tue, 14 Jul 2020 08:51:40: 8000000 INFO @ Tue, 14 Jul 2020 08:51:42: 17000000 INFO @ Tue, 14 Jul 2020 08:51:43: 12000000 INFO @ Tue, 14 Jul 2020 08:51:47: 9000000 INFO @ Tue, 14 Jul 2020 08:51:50: 18000000 INFO @ Tue, 14 Jul 2020 08:51:50: 13000000 INFO @ Tue, 14 Jul 2020 08:51:54: 10000000 INFO @ Tue, 14 Jul 2020 08:51:58: 19000000 INFO @ Tue, 14 Jul 2020 08:51:58: 14000000 INFO @ Tue, 14 Jul 2020 08:52:02: 11000000 INFO @ Tue, 14 Jul 2020 08:52:06: 20000000 INFO @ Tue, 14 Jul 2020 08:52:06: 15000000 INFO @ Tue, 14 Jul 2020 08:52:10: 12000000 INFO @ Tue, 14 Jul 2020 08:52:13: 21000000 INFO @ Tue, 14 Jul 2020 08:52:14: 16000000 INFO @ Tue, 14 Jul 2020 08:52:17: 13000000 INFO @ Tue, 14 Jul 2020 08:52:21: 22000000 INFO @ Tue, 14 Jul 2020 08:52:22: 17000000 INFO @ Tue, 14 Jul 2020 08:52:25: 14000000 INFO @ Tue, 14 Jul 2020 08:52:29: 23000000 INFO @ Tue, 14 Jul 2020 08:52:30: 18000000 INFO @ Tue, 14 Jul 2020 08:52:32: 15000000 INFO @ Tue, 14 Jul 2020 08:52:37: 24000000 INFO @ Tue, 14 Jul 2020 08:52:38: 19000000 INFO @ Tue, 14 Jul 2020 08:52:40: 16000000 INFO @ Tue, 14 Jul 2020 08:52:45: 25000000 INFO @ Tue, 14 Jul 2020 08:52:46: 20000000 INFO @ Tue, 14 Jul 2020 08:52:47: 17000000 INFO @ Tue, 14 Jul 2020 08:52:52: 26000000 INFO @ Tue, 14 Jul 2020 08:52:55: 18000000 INFO @ Tue, 14 Jul 2020 08:52:55: 21000000 INFO @ Tue, 14 Jul 2020 08:53:00: 27000000 INFO @ Tue, 14 Jul 2020 08:53:02: 19000000 INFO @ Tue, 14 Jul 2020 08:53:03: 22000000 INFO @ Tue, 14 Jul 2020 08:53:08: 28000000 INFO @ Tue, 14 Jul 2020 08:53:10: 20000000 INFO @ Tue, 14 Jul 2020 08:53:11: 23000000 INFO @ Tue, 14 Jul 2020 08:53:16: 29000000 INFO @ Tue, 14 Jul 2020 08:53:17: 21000000 INFO @ Tue, 14 Jul 2020 08:53:19: 24000000 INFO @ Tue, 14 Jul 2020 08:53:24: 30000000 INFO @ Tue, 14 Jul 2020 08:53:25: 22000000 INFO @ Tue, 14 Jul 2020 08:53:26: 25000000 INFO @ Tue, 14 Jul 2020 08:53:32: 23000000 INFO @ Tue, 14 Jul 2020 08:53:32: 31000000 INFO @ Tue, 14 Jul 2020 08:53:34: 26000000 INFO @ Tue, 14 Jul 2020 08:53:40: 24000000 INFO @ Tue, 14 Jul 2020 08:53:40: 32000000 INFO @ Tue, 14 Jul 2020 08:53:42: 27000000 INFO @ Tue, 14 Jul 2020 08:53:47: 25000000 INFO @ Tue, 14 Jul 2020 08:53:48: 33000000 INFO @ Tue, 14 Jul 2020 08:53:49: 28000000 INFO @ Tue, 14 Jul 2020 08:53:55: 26000000 INFO @ Tue, 14 Jul 2020 08:53:56: 34000000 INFO @ Tue, 14 Jul 2020 08:53:57: 29000000 INFO @ Tue, 14 Jul 2020 08:54:02: 27000000 INFO @ Tue, 14 Jul 2020 08:54:04: 30000000 INFO @ Tue, 14 Jul 2020 08:54:04: 35000000 INFO @ Tue, 14 Jul 2020 08:54:09: 28000000 INFO @ Tue, 14 Jul 2020 08:54:12: 31000000 INFO @ Tue, 14 Jul 2020 08:54:12: 36000000 INFO @ Tue, 14 Jul 2020 08:54:17: 29000000 INFO @ Tue, 14 Jul 2020 08:54:19: 32000000 INFO @ Tue, 14 Jul 2020 08:54:20: 37000000 INFO @ Tue, 14 Jul 2020 08:54:24: 30000000 INFO @ Tue, 14 Jul 2020 08:54:27: 33000000 INFO @ Tue, 14 Jul 2020 08:54:28: 38000000 INFO @ Tue, 14 Jul 2020 08:54:32: 31000000 INFO @ Tue, 14 Jul 2020 08:54:34: 34000000 INFO @ Tue, 14 Jul 2020 08:54:36: 39000000 INFO @ Tue, 14 Jul 2020 08:54:39: 32000000 INFO @ Tue, 14 Jul 2020 08:54:42: 35000000 INFO @ Tue, 14 Jul 2020 08:54:44: 40000000 INFO @ Tue, 14 Jul 2020 08:54:46: 33000000 INFO @ Tue, 14 Jul 2020 08:54:49: 36000000 INFO @ Tue, 14 Jul 2020 08:54:52: 41000000 INFO @ Tue, 14 Jul 2020 08:54:54: 34000000 INFO @ Tue, 14 Jul 2020 08:54:56: 37000000 INFO @ Tue, 14 Jul 2020 08:55:00: 42000000 INFO @ Tue, 14 Jul 2020 08:55:01: 35000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:55:04: 38000000 INFO @ Tue, 14 Jul 2020 08:55:08: 43000000 INFO @ Tue, 14 Jul 2020 08:55:09: 36000000 INFO @ Tue, 14 Jul 2020 08:55:11: 39000000 INFO @ Tue, 14 Jul 2020 08:55:16: 44000000 INFO @ Tue, 14 Jul 2020 08:55:16: 37000000 INFO @ Tue, 14 Jul 2020 08:55:19: 40000000 INFO @ Tue, 14 Jul 2020 08:55:23: 45000000 INFO @ Tue, 14 Jul 2020 08:55:24: 38000000 INFO @ Tue, 14 Jul 2020 08:55:26: 41000000 INFO @ Tue, 14 Jul 2020 08:55:31: 39000000 INFO @ Tue, 14 Jul 2020 08:55:31: 46000000 INFO @ Tue, 14 Jul 2020 08:55:34: 42000000 INFO @ Tue, 14 Jul 2020 08:55:39: 40000000 INFO @ Tue, 14 Jul 2020 08:55:39: 47000000 INFO @ Tue, 14 Jul 2020 08:55:41: 43000000 INFO @ Tue, 14 Jul 2020 08:55:46: 41000000 INFO @ Tue, 14 Jul 2020 08:55:46: 48000000 INFO @ Tue, 14 Jul 2020 08:55:49: 44000000 INFO @ Tue, 14 Jul 2020 08:55:54: 42000000 INFO @ Tue, 14 Jul 2020 08:55:54: 49000000 INFO @ Tue, 14 Jul 2020 08:55:56: 45000000 INFO @ Tue, 14 Jul 2020 08:56:01: 43000000 INFO @ Tue, 14 Jul 2020 08:56:02: 50000000 INFO @ Tue, 14 Jul 2020 08:56:04: 46000000 INFO @ Tue, 14 Jul 2020 08:56:08: 44000000 INFO @ Tue, 14 Jul 2020 08:56:10: 51000000 INFO @ Tue, 14 Jul 2020 08:56:11: 47000000 INFO @ Tue, 14 Jul 2020 08:56:16: 45000000 INFO @ Tue, 14 Jul 2020 08:56:17: 52000000 INFO @ Tue, 14 Jul 2020 08:56:18: 48000000 INFO @ Tue, 14 Jul 2020 08:56:23: 46000000 INFO @ Tue, 14 Jul 2020 08:56:25: 53000000 INFO @ Tue, 14 Jul 2020 08:56:26: 49000000 INFO @ Tue, 14 Jul 2020 08:56:31: 47000000 INFO @ Tue, 14 Jul 2020 08:56:33: 54000000 INFO @ Tue, 14 Jul 2020 08:56:33: 50000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:56:38: 48000000 INFO @ Tue, 14 Jul 2020 08:56:38: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:56:38: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:56:38: #1 total tags in treatment: 25179823 INFO @ Tue, 14 Jul 2020 08:56:38: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:56:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:56:39: #1 tags after filtering in treatment: 21771906 INFO @ Tue, 14 Jul 2020 08:56:39: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 14 Jul 2020 08:56:39: #1 finished! INFO @ Tue, 14 Jul 2020 08:56:39: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:56:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:56:40: #2 number of paired peaks: 43 WARNING @ Tue, 14 Jul 2020 08:56:40: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:56:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:56:41: 51000000 INFO @ Tue, 14 Jul 2020 08:56:46: 49000000 INFO @ Tue, 14 Jul 2020 08:56:48: 52000000 INFO @ Tue, 14 Jul 2020 08:56:53: 50000000 INFO @ Tue, 14 Jul 2020 08:56:56: 53000000 INFO @ Tue, 14 Jul 2020 08:57:00: 51000000 INFO @ Tue, 14 Jul 2020 08:57:03: 54000000 INFO @ Tue, 14 Jul 2020 08:57:08: 52000000 INFO @ Tue, 14 Jul 2020 08:57:08: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:57:08: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:57:08: #1 total tags in treatment: 25179823 INFO @ Tue, 14 Jul 2020 08:57:08: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:57:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:57:09: #1 tags after filtering in treatment: 21771906 INFO @ Tue, 14 Jul 2020 08:57:09: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 14 Jul 2020 08:57:09: #1 finished! INFO @ Tue, 14 Jul 2020 08:57:09: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:57:09: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:57:10: #2 number of paired peaks: 43 WARNING @ Tue, 14 Jul 2020 08:57:10: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:57:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:57:15: 53000000 INFO @ Tue, 14 Jul 2020 08:57:22: 54000000 INFO @ Tue, 14 Jul 2020 08:57:27: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:57:27: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:57:27: #1 total tags in treatment: 25179823 INFO @ Tue, 14 Jul 2020 08:57:27: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:57:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:57:27: #1 tags after filtering in treatment: 21771906 INFO @ Tue, 14 Jul 2020 08:57:27: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 14 Jul 2020 08:57:27: #1 finished! INFO @ Tue, 14 Jul 2020 08:57:27: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:57:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:57:28: #2 number of paired peaks: 43 WARNING @ Tue, 14 Jul 2020 08:57:28: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:57:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736464/SRX5736464.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling