Job ID = 6626475 SRX = SRX5736459 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 21739519 spots for SRR8956871/SRR8956871.sra Written 21739519 spots for SRR8956871/SRR8956871.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626787 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:08:56 21739519 reads; of these: 21739519 (100.00%) were paired; of these: 2808934 (12.92%) aligned concordantly 0 times 11508829 (52.94%) aligned concordantly exactly 1 time 7421756 (34.14%) aligned concordantly >1 times ---- 2808934 pairs aligned concordantly 0 times; of these: 483051 (17.20%) aligned discordantly 1 time ---- 2325883 pairs aligned 0 times concordantly or discordantly; of these: 4651766 mates make up the pairs; of these: 3300514 (70.95%) aligned 0 times 486178 (10.45%) aligned exactly 1 time 865074 (18.60%) aligned >1 times 92.41% overall alignment rate Time searching: 01:08:56 Overall time: 01:08:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1291208 / 19311234 = 0.0669 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:29:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:29:07: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:29:07: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:29:13: 1000000 INFO @ Tue, 14 Jul 2020 08:29:18: 2000000 INFO @ Tue, 14 Jul 2020 08:29:24: 3000000 INFO @ Tue, 14 Jul 2020 08:29:29: 4000000 INFO @ Tue, 14 Jul 2020 08:29:34: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:29:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:29:37: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:29:37: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:29:39: 6000000 INFO @ Tue, 14 Jul 2020 08:29:43: 1000000 INFO @ Tue, 14 Jul 2020 08:29:44: 7000000 INFO @ Tue, 14 Jul 2020 08:29:48: 2000000 INFO @ Tue, 14 Jul 2020 08:29:50: 8000000 INFO @ Tue, 14 Jul 2020 08:29:54: 3000000 INFO @ Tue, 14 Jul 2020 08:29:55: 9000000 INFO @ Tue, 14 Jul 2020 08:29:59: 4000000 INFO @ Tue, 14 Jul 2020 08:30:00: 10000000 INFO @ Tue, 14 Jul 2020 08:30:05: 5000000 INFO @ Tue, 14 Jul 2020 08:30:05: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:30:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:30:07: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:30:07: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:30:10: 6000000 INFO @ Tue, 14 Jul 2020 08:30:10: 12000000 INFO @ Tue, 14 Jul 2020 08:30:13: 1000000 INFO @ Tue, 14 Jul 2020 08:30:16: 7000000 INFO @ Tue, 14 Jul 2020 08:30:16: 13000000 INFO @ Tue, 14 Jul 2020 08:30:18: 2000000 INFO @ Tue, 14 Jul 2020 08:30:21: 14000000 INFO @ Tue, 14 Jul 2020 08:30:21: 8000000 INFO @ Tue, 14 Jul 2020 08:30:23: 3000000 INFO @ Tue, 14 Jul 2020 08:30:26: 15000000 INFO @ Tue, 14 Jul 2020 08:30:27: 9000000 INFO @ Tue, 14 Jul 2020 08:30:29: 4000000 INFO @ Tue, 14 Jul 2020 08:30:31: 16000000 INFO @ Tue, 14 Jul 2020 08:30:32: 10000000 INFO @ Tue, 14 Jul 2020 08:30:34: 5000000 INFO @ Tue, 14 Jul 2020 08:30:36: 17000000 INFO @ Tue, 14 Jul 2020 08:30:37: 11000000 INFO @ Tue, 14 Jul 2020 08:30:39: 6000000 INFO @ Tue, 14 Jul 2020 08:30:42: 18000000 INFO @ Tue, 14 Jul 2020 08:30:43: 12000000 INFO @ Tue, 14 Jul 2020 08:30:44: 7000000 INFO @ Tue, 14 Jul 2020 08:30:47: 19000000 INFO @ Tue, 14 Jul 2020 08:30:48: 13000000 INFO @ Tue, 14 Jul 2020 08:30:50: 8000000 INFO @ Tue, 14 Jul 2020 08:30:52: 20000000 INFO @ Tue, 14 Jul 2020 08:30:54: 14000000 INFO @ Tue, 14 Jul 2020 08:30:55: 9000000 INFO @ Tue, 14 Jul 2020 08:30:57: 21000000 INFO @ Tue, 14 Jul 2020 08:30:59: 15000000 INFO @ Tue, 14 Jul 2020 08:31:00: 10000000 INFO @ Tue, 14 Jul 2020 08:31:02: 22000000 INFO @ Tue, 14 Jul 2020 08:31:04: 16000000 INFO @ Tue, 14 Jul 2020 08:31:05: 11000000 INFO @ Tue, 14 Jul 2020 08:31:07: 23000000 INFO @ Tue, 14 Jul 2020 08:31:09: 17000000 INFO @ Tue, 14 Jul 2020 08:31:11: 12000000 INFO @ Tue, 14 Jul 2020 08:31:13: 24000000 INFO @ Tue, 14 Jul 2020 08:31:14: 18000000 INFO @ Tue, 14 Jul 2020 08:31:17: 13000000 INFO @ Tue, 14 Jul 2020 08:31:18: 25000000 INFO @ Tue, 14 Jul 2020 08:31:19: 19000000 INFO @ Tue, 14 Jul 2020 08:31:22: 14000000 INFO @ Tue, 14 Jul 2020 08:31:23: 26000000 INFO @ Tue, 14 Jul 2020 08:31:25: 20000000 INFO @ Tue, 14 Jul 2020 08:31:27: 15000000 INFO @ Tue, 14 Jul 2020 08:31:28: 27000000 INFO @ Tue, 14 Jul 2020 08:31:30: 21000000 INFO @ Tue, 14 Jul 2020 08:31:32: 16000000 INFO @ Tue, 14 Jul 2020 08:31:33: 28000000 INFO @ Tue, 14 Jul 2020 08:31:35: 22000000 INFO @ Tue, 14 Jul 2020 08:31:38: 17000000 INFO @ Tue, 14 Jul 2020 08:31:38: 29000000 INFO @ Tue, 14 Jul 2020 08:31:40: 23000000 INFO @ Tue, 14 Jul 2020 08:31:43: 18000000 INFO @ Tue, 14 Jul 2020 08:31:43: 30000000 INFO @ Tue, 14 Jul 2020 08:31:46: 24000000 INFO @ Tue, 14 Jul 2020 08:31:48: 19000000 INFO @ Tue, 14 Jul 2020 08:31:48: 31000000 INFO @ Tue, 14 Jul 2020 08:31:51: 25000000 INFO @ Tue, 14 Jul 2020 08:31:53: 20000000 INFO @ Tue, 14 Jul 2020 08:31:53: 32000000 INFO @ Tue, 14 Jul 2020 08:31:56: 26000000 INFO @ Tue, 14 Jul 2020 08:31:58: 21000000 INFO @ Tue, 14 Jul 2020 08:31:59: 33000000 INFO @ Tue, 14 Jul 2020 08:32:02: 27000000 INFO @ Tue, 14 Jul 2020 08:32:03: 22000000 INFO @ Tue, 14 Jul 2020 08:32:04: 34000000 INFO @ Tue, 14 Jul 2020 08:32:07: 28000000 INFO @ Tue, 14 Jul 2020 08:32:08: 23000000 INFO @ Tue, 14 Jul 2020 08:32:09: 35000000 INFO @ Tue, 14 Jul 2020 08:32:12: 29000000 INFO @ Tue, 14 Jul 2020 08:32:13: 24000000 INFO @ Tue, 14 Jul 2020 08:32:14: 36000000 INFO @ Tue, 14 Jul 2020 08:32:17: 30000000 INFO @ Tue, 14 Jul 2020 08:32:18: 25000000 INFO @ Tue, 14 Jul 2020 08:32:19: 37000000 INFO @ Tue, 14 Jul 2020 08:32:22: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:32:22: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:32:22: #1 total tags in treatment: 17646634 INFO @ Tue, 14 Jul 2020 08:32:22: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:32:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:32:23: 31000000 INFO @ Tue, 14 Jul 2020 08:32:23: #1 tags after filtering in treatment: 15378039 INFO @ Tue, 14 Jul 2020 08:32:23: #1 Redundant rate of treatment: 0.13 INFO @ Tue, 14 Jul 2020 08:32:23: #1 finished! INFO @ Tue, 14 Jul 2020 08:32:23: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:32:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:32:23: 26000000 INFO @ Tue, 14 Jul 2020 08:32:24: #2 number of paired peaks: 63 WARNING @ Tue, 14 Jul 2020 08:32:24: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:32:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:32:28: 32000000 INFO @ Tue, 14 Jul 2020 08:32:28: 27000000 INFO @ Tue, 14 Jul 2020 08:32:33: 33000000 INFO @ Tue, 14 Jul 2020 08:32:34: 28000000 INFO @ Tue, 14 Jul 2020 08:32:38: 34000000 INFO @ Tue, 14 Jul 2020 08:32:39: 29000000 INFO @ Tue, 14 Jul 2020 08:32:44: 35000000 INFO @ Tue, 14 Jul 2020 08:32:44: 30000000 INFO @ Tue, 14 Jul 2020 08:32:49: 36000000 INFO @ Tue, 14 Jul 2020 08:32:49: 31000000 INFO @ Tue, 14 Jul 2020 08:32:54: 32000000 INFO @ Tue, 14 Jul 2020 08:32:54: 37000000 INFO @ Tue, 14 Jul 2020 08:32:57: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:32:57: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:32:57: #1 total tags in treatment: 17646634 INFO @ Tue, 14 Jul 2020 08:32:57: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:32:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:32:58: #1 tags after filtering in treatment: 15378039 INFO @ Tue, 14 Jul 2020 08:32:58: #1 Redundant rate of treatment: 0.13 INFO @ Tue, 14 Jul 2020 08:32:58: #1 finished! INFO @ Tue, 14 Jul 2020 08:32:58: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:32:58: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:32:59: #2 number of paired peaks: 63 WARNING @ Tue, 14 Jul 2020 08:32:59: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:32:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:32:59: 33000000 INFO @ Tue, 14 Jul 2020 08:33:04: 34000000 INFO @ Tue, 14 Jul 2020 08:33:09: 35000000 INFO @ Tue, 14 Jul 2020 08:33:14: 36000000 INFO @ Tue, 14 Jul 2020 08:33:19: 37000000 INFO @ Tue, 14 Jul 2020 08:33:23: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:33:23: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:33:23: #1 total tags in treatment: 17646634 INFO @ Tue, 14 Jul 2020 08:33:23: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:33:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:33:23: #1 tags after filtering in treatment: 15378039 INFO @ Tue, 14 Jul 2020 08:33:23: #1 Redundant rate of treatment: 0.13 INFO @ Tue, 14 Jul 2020 08:33:23: #1 finished! INFO @ Tue, 14 Jul 2020 08:33:23: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:33:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:33:24: #2 number of paired peaks: 63 WARNING @ Tue, 14 Jul 2020 08:33:24: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:33:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736459/SRX5736459.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。