Job ID = 6528398 SRX = SRX5717045 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:56:54 prefetch.2.10.7: 1) Downloading 'SRR8936406'... 2020-06-29T14:56:54 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:58:17 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:58:17 prefetch.2.10.7: 1) 'SRR8936406' was downloaded successfully Read 27086615 spots for SRR8936406/SRR8936406.sra Written 27086615 spots for SRR8936406/SRR8936406.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:06 27086615 reads; of these: 27086615 (100.00%) were unpaired; of these: 2122425 (7.84%) aligned 0 times 19472747 (71.89%) aligned exactly 1 time 5491443 (20.27%) aligned >1 times 92.16% overall alignment rate Time searching: 00:07:06 Overall time: 00:07:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7266113 / 24964190 = 0.2911 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:16:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:16:52: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:16:52: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:16:59: 1000000 INFO @ Tue, 30 Jun 2020 00:17:06: 2000000 INFO @ Tue, 30 Jun 2020 00:17:12: 3000000 INFO @ Tue, 30 Jun 2020 00:17:19: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:17:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:17:22: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:17:22: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:17:26: 5000000 INFO @ Tue, 30 Jun 2020 00:17:28: 1000000 INFO @ Tue, 30 Jun 2020 00:17:33: 6000000 INFO @ Tue, 30 Jun 2020 00:17:35: 2000000 INFO @ Tue, 30 Jun 2020 00:17:40: 7000000 INFO @ Tue, 30 Jun 2020 00:17:41: 3000000 INFO @ Tue, 30 Jun 2020 00:17:47: 8000000 INFO @ Tue, 30 Jun 2020 00:17:48: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:17:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:17:52: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:17:52: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:17:54: 5000000 INFO @ Tue, 30 Jun 2020 00:17:55: 9000000 INFO @ Tue, 30 Jun 2020 00:17:59: 1000000 INFO @ Tue, 30 Jun 2020 00:18:00: 6000000 INFO @ Tue, 30 Jun 2020 00:18:02: 10000000 INFO @ Tue, 30 Jun 2020 00:18:05: 2000000 INFO @ Tue, 30 Jun 2020 00:18:07: 7000000 INFO @ Tue, 30 Jun 2020 00:18:09: 11000000 INFO @ Tue, 30 Jun 2020 00:18:11: 3000000 INFO @ Tue, 30 Jun 2020 00:18:13: 8000000 INFO @ Tue, 30 Jun 2020 00:18:16: 12000000 INFO @ Tue, 30 Jun 2020 00:18:18: 4000000 INFO @ Tue, 30 Jun 2020 00:18:20: 9000000 INFO @ Tue, 30 Jun 2020 00:18:23: 13000000 INFO @ Tue, 30 Jun 2020 00:18:24: 5000000 INFO @ Tue, 30 Jun 2020 00:18:26: 10000000 INFO @ Tue, 30 Jun 2020 00:18:31: 14000000 INFO @ Tue, 30 Jun 2020 00:18:31: 6000000 INFO @ Tue, 30 Jun 2020 00:18:33: 11000000 INFO @ Tue, 30 Jun 2020 00:18:37: 7000000 INFO @ Tue, 30 Jun 2020 00:18:38: 15000000 INFO @ Tue, 30 Jun 2020 00:18:39: 12000000 INFO @ Tue, 30 Jun 2020 00:18:44: 8000000 INFO @ Tue, 30 Jun 2020 00:18:45: 16000000 INFO @ Tue, 30 Jun 2020 00:18:45: 13000000 INFO @ Tue, 30 Jun 2020 00:18:50: 9000000 INFO @ Tue, 30 Jun 2020 00:18:52: 14000000 INFO @ Tue, 30 Jun 2020 00:18:52: 17000000 INFO @ Tue, 30 Jun 2020 00:18:56: 10000000 INFO @ Tue, 30 Jun 2020 00:18:57: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:18:57: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:18:57: #1 total tags in treatment: 17698077 INFO @ Tue, 30 Jun 2020 00:18:57: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:18:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:18:58: #1 tags after filtering in treatment: 17698077 INFO @ Tue, 30 Jun 2020 00:18:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:18:58: #1 finished! INFO @ Tue, 30 Jun 2020 00:18:58: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:18:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:18:58: 15000000 INFO @ Tue, 30 Jun 2020 00:18:59: #2 number of paired peaks: 43 WARNING @ Tue, 30 Jun 2020 00:18:59: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:18:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:19:03: 11000000 INFO @ Tue, 30 Jun 2020 00:19:05: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:19:09: 12000000 INFO @ Tue, 30 Jun 2020 00:19:11: 17000000 INFO @ Tue, 30 Jun 2020 00:19:15: 13000000 INFO @ Tue, 30 Jun 2020 00:19:15: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:19:15: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:19:15: #1 total tags in treatment: 17698077 INFO @ Tue, 30 Jun 2020 00:19:15: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:19:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:19:15: #1 tags after filtering in treatment: 17698077 INFO @ Tue, 30 Jun 2020 00:19:15: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:19:15: #1 finished! INFO @ Tue, 30 Jun 2020 00:19:15: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:19:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:19:17: #2 number of paired peaks: 43 WARNING @ Tue, 30 Jun 2020 00:19:17: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:19:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:19:21: 14000000 INFO @ Tue, 30 Jun 2020 00:19:27: 15000000 INFO @ Tue, 30 Jun 2020 00:19:33: 16000000 INFO @ Tue, 30 Jun 2020 00:19:38: 17000000 INFO @ Tue, 30 Jun 2020 00:19:42: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:19:42: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:19:42: #1 total tags in treatment: 17698077 INFO @ Tue, 30 Jun 2020 00:19:42: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:19:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:19:43: #1 tags after filtering in treatment: 17698077 INFO @ Tue, 30 Jun 2020 00:19:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:19:43: #1 finished! INFO @ Tue, 30 Jun 2020 00:19:43: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:19:43: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 00:19:44: #2 number of paired peaks: 43 WARNING @ Tue, 30 Jun 2020 00:19:44: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:19:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717045/SRX5717045.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling