Job ID = 6528396 SRX = SRX5717038 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:56:13 prefetch.2.10.7: 1) Downloading 'SRR8936399'... 2020-06-29T14:56:13 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:57:55 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:57:55 prefetch.2.10.7: 1) 'SRR8936399' was downloaded successfully Read 27161795 spots for SRR8936399/SRR8936399.sra Written 27161795 spots for SRR8936399/SRR8936399.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:48 27161795 reads; of these: 27161795 (100.00%) were unpaired; of these: 3560251 (13.11%) aligned 0 times 15473133 (56.97%) aligned exactly 1 time 8128411 (29.93%) aligned >1 times 86.89% overall alignment rate Time searching: 00:08:48 Overall time: 00:08:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5879632 / 23601544 = 0.2491 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:18:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:18:15: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:18:15: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:18:20: 1000000 INFO @ Tue, 30 Jun 2020 00:18:25: 2000000 INFO @ Tue, 30 Jun 2020 00:18:30: 3000000 INFO @ Tue, 30 Jun 2020 00:18:35: 4000000 INFO @ Tue, 30 Jun 2020 00:18:39: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:18:44: 6000000 INFO @ Tue, 30 Jun 2020 00:18:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:18:45: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:18:45: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:18:49: 7000000 INFO @ Tue, 30 Jun 2020 00:18:50: 1000000 INFO @ Tue, 30 Jun 2020 00:18:54: 8000000 INFO @ Tue, 30 Jun 2020 00:18:55: 2000000 INFO @ Tue, 30 Jun 2020 00:18:59: 9000000 INFO @ Tue, 30 Jun 2020 00:19:00: 3000000 INFO @ Tue, 30 Jun 2020 00:19:04: 10000000 INFO @ Tue, 30 Jun 2020 00:19:05: 4000000 INFO @ Tue, 30 Jun 2020 00:19:09: 11000000 INFO @ Tue, 30 Jun 2020 00:19:11: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:19:14: 12000000 INFO @ Tue, 30 Jun 2020 00:19:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:19:15: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:19:15: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:19:16: 6000000 INFO @ Tue, 30 Jun 2020 00:19:19: 13000000 INFO @ Tue, 30 Jun 2020 00:19:20: 1000000 INFO @ Tue, 30 Jun 2020 00:19:21: 7000000 INFO @ Tue, 30 Jun 2020 00:19:24: 14000000 INFO @ Tue, 30 Jun 2020 00:19:25: 2000000 INFO @ Tue, 30 Jun 2020 00:19:26: 8000000 INFO @ Tue, 30 Jun 2020 00:19:30: 15000000 INFO @ Tue, 30 Jun 2020 00:19:31: 3000000 INFO @ Tue, 30 Jun 2020 00:19:31: 9000000 INFO @ Tue, 30 Jun 2020 00:19:35: 16000000 INFO @ Tue, 30 Jun 2020 00:19:36: 4000000 INFO @ Tue, 30 Jun 2020 00:19:36: 10000000 INFO @ Tue, 30 Jun 2020 00:19:40: 17000000 INFO @ Tue, 30 Jun 2020 00:19:41: 5000000 INFO @ Tue, 30 Jun 2020 00:19:41: 11000000 INFO @ Tue, 30 Jun 2020 00:19:43: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:19:43: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:19:43: #1 total tags in treatment: 17721912 INFO @ Tue, 30 Jun 2020 00:19:43: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:19:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:19:44: #1 tags after filtering in treatment: 17721912 INFO @ Tue, 30 Jun 2020 00:19:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:19:44: #1 finished! INFO @ Tue, 30 Jun 2020 00:19:44: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:19:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:19:45: #2 number of paired peaks: 35 WARNING @ Tue, 30 Jun 2020 00:19:45: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:19:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:19:46: 6000000 INFO @ Tue, 30 Jun 2020 00:19:46: 12000000 INFO @ Tue, 30 Jun 2020 00:19:51: 7000000 INFO @ Tue, 30 Jun 2020 00:19:51: 13000000 INFO @ Tue, 30 Jun 2020 00:19:56: 8000000 INFO @ Tue, 30 Jun 2020 00:19:56: 14000000 INFO @ Tue, 30 Jun 2020 00:20:01: 9000000 INFO @ Tue, 30 Jun 2020 00:20:01: 15000000 INFO @ Tue, 30 Jun 2020 00:20:06: 10000000 INFO @ Tue, 30 Jun 2020 00:20:06: 16000000 INFO @ Tue, 30 Jun 2020 00:20:11: 11000000 INFO @ Tue, 30 Jun 2020 00:20:11: 17000000 INFO @ Tue, 30 Jun 2020 00:20:15: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:20:15: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:20:15: #1 total tags in treatment: 17721912 INFO @ Tue, 30 Jun 2020 00:20:15: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:20:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:20:15: #1 tags after filtering in treatment: 17721912 INFO @ Tue, 30 Jun 2020 00:20:15: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:20:15: #1 finished! INFO @ Tue, 30 Jun 2020 00:20:15: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:20:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:20:16: 12000000 INFO @ Tue, 30 Jun 2020 00:20:17: #2 number of paired peaks: 35 WARNING @ Tue, 30 Jun 2020 00:20:17: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:20:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:20:21: 13000000 INFO @ Tue, 30 Jun 2020 00:20:26: 14000000 INFO @ Tue, 30 Jun 2020 00:20:31: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:20:36: 16000000 INFO @ Tue, 30 Jun 2020 00:20:41: 17000000 INFO @ Tue, 30 Jun 2020 00:20:45: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:20:45: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:20:45: #1 total tags in treatment: 17721912 INFO @ Tue, 30 Jun 2020 00:20:45: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:20:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:20:45: #1 tags after filtering in treatment: 17721912 INFO @ Tue, 30 Jun 2020 00:20:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:20:45: #1 finished! INFO @ Tue, 30 Jun 2020 00:20:45: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:20:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:20:46: #2 number of paired peaks: 35 WARNING @ Tue, 30 Jun 2020 00:20:46: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:20:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717038/SRX5717038.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。