Job ID = 6528392 SRX = SRX5717028 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:22:18 prefetch.2.10.7: 1) Downloading 'SRR8936388'... 2020-06-29T15:22:18 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:26:54 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:26:54 prefetch.2.10.7: 1) 'SRR8936388' was downloaded successfully Read 62789544 spots for SRR8936388/SRR8936388.sra Written 62789544 spots for SRR8936388/SRR8936388.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:29:02 62789544 reads; of these: 62789544 (100.00%) were unpaired; of these: 8212780 (13.08%) aligned 0 times 21329926 (33.97%) aligned exactly 1 time 33246838 (52.95%) aligned >1 times 86.92% overall alignment rate Time searching: 00:29:02 Overall time: 00:29:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 26171234 / 54576764 = 0.4795 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:26:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:26:51: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:26:51: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:26:57: 1000000 INFO @ Tue, 30 Jun 2020 01:27:03: 2000000 INFO @ Tue, 30 Jun 2020 01:27:10: 3000000 INFO @ Tue, 30 Jun 2020 01:27:16: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:27:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:27:21: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:27:21: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:27:22: 5000000 INFO @ Tue, 30 Jun 2020 01:27:27: 1000000 INFO @ Tue, 30 Jun 2020 01:27:29: 6000000 INFO @ Tue, 30 Jun 2020 01:27:34: 2000000 INFO @ Tue, 30 Jun 2020 01:27:35: 7000000 INFO @ Tue, 30 Jun 2020 01:27:40: 3000000 INFO @ Tue, 30 Jun 2020 01:27:42: 8000000 INFO @ Tue, 30 Jun 2020 01:27:46: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:27:49: 9000000 INFO @ Tue, 30 Jun 2020 01:27:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:27:51: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:27:51: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:27:52: 5000000 INFO @ Tue, 30 Jun 2020 01:27:55: 10000000 INFO @ Tue, 30 Jun 2020 01:27:57: 1000000 INFO @ Tue, 30 Jun 2020 01:27:59: 6000000 INFO @ Tue, 30 Jun 2020 01:28:02: 11000000 INFO @ Tue, 30 Jun 2020 01:28:04: 2000000 INFO @ Tue, 30 Jun 2020 01:28:05: 7000000 INFO @ Tue, 30 Jun 2020 01:28:09: 12000000 INFO @ Tue, 30 Jun 2020 01:28:11: 3000000 INFO @ Tue, 30 Jun 2020 01:28:12: 8000000 INFO @ Tue, 30 Jun 2020 01:28:16: 13000000 INFO @ Tue, 30 Jun 2020 01:28:17: 4000000 INFO @ Tue, 30 Jun 2020 01:28:18: 9000000 INFO @ Tue, 30 Jun 2020 01:28:23: 14000000 INFO @ Tue, 30 Jun 2020 01:28:23: 5000000 INFO @ Tue, 30 Jun 2020 01:28:25: 10000000 INFO @ Tue, 30 Jun 2020 01:28:30: 6000000 INFO @ Tue, 30 Jun 2020 01:28:30: 15000000 INFO @ Tue, 30 Jun 2020 01:28:31: 11000000 INFO @ Tue, 30 Jun 2020 01:28:36: 7000000 INFO @ Tue, 30 Jun 2020 01:28:37: 16000000 INFO @ Tue, 30 Jun 2020 01:28:38: 12000000 INFO @ Tue, 30 Jun 2020 01:28:43: 8000000 INFO @ Tue, 30 Jun 2020 01:28:44: 17000000 INFO @ Tue, 30 Jun 2020 01:28:44: 13000000 INFO @ Tue, 30 Jun 2020 01:28:49: 9000000 INFO @ Tue, 30 Jun 2020 01:28:50: 18000000 INFO @ Tue, 30 Jun 2020 01:28:51: 14000000 INFO @ Tue, 30 Jun 2020 01:28:56: 10000000 INFO @ Tue, 30 Jun 2020 01:28:57: 19000000 INFO @ Tue, 30 Jun 2020 01:28:57: 15000000 INFO @ Tue, 30 Jun 2020 01:29:02: 11000000 INFO @ Tue, 30 Jun 2020 01:29:04: 16000000 INFO @ Tue, 30 Jun 2020 01:29:04: 20000000 INFO @ Tue, 30 Jun 2020 01:29:08: 12000000 INFO @ Tue, 30 Jun 2020 01:29:10: 17000000 INFO @ Tue, 30 Jun 2020 01:29:11: 21000000 INFO @ Tue, 30 Jun 2020 01:29:15: 13000000 INFO @ Tue, 30 Jun 2020 01:29:17: 18000000 INFO @ Tue, 30 Jun 2020 01:29:18: 22000000 INFO @ Tue, 30 Jun 2020 01:29:21: 14000000 INFO @ Tue, 30 Jun 2020 01:29:23: 19000000 INFO @ Tue, 30 Jun 2020 01:29:25: 23000000 INFO @ Tue, 30 Jun 2020 01:29:28: 15000000 INFO @ Tue, 30 Jun 2020 01:29:29: 20000000 INFO @ Tue, 30 Jun 2020 01:29:32: 24000000 INFO @ Tue, 30 Jun 2020 01:29:34: 16000000 INFO @ Tue, 30 Jun 2020 01:29:36: 21000000 INFO @ Tue, 30 Jun 2020 01:29:39: 25000000 INFO @ Tue, 30 Jun 2020 01:29:40: 17000000 INFO @ Tue, 30 Jun 2020 01:29:42: 22000000 INFO @ Tue, 30 Jun 2020 01:29:46: 26000000 INFO @ Tue, 30 Jun 2020 01:29:47: 18000000 INFO @ Tue, 30 Jun 2020 01:29:49: 23000000 INFO @ Tue, 30 Jun 2020 01:29:53: 27000000 INFO @ Tue, 30 Jun 2020 01:29:53: 19000000 INFO @ Tue, 30 Jun 2020 01:29:55: 24000000 INFO @ Tue, 30 Jun 2020 01:30:00: 20000000 INFO @ Tue, 30 Jun 2020 01:30:00: 28000000 INFO @ Tue, 30 Jun 2020 01:30:02: 25000000 INFO @ Tue, 30 Jun 2020 01:30:03: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 01:30:03: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 01:30:03: #1 total tags in treatment: 28405530 INFO @ Tue, 30 Jun 2020 01:30:03: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:30:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:30:03: #1 tags after filtering in treatment: 28405530 INFO @ Tue, 30 Jun 2020 01:30:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:30:03: #1 finished! INFO @ Tue, 30 Jun 2020 01:30:03: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:30:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:30:05: #2 number of paired peaks: 3 WARNING @ Tue, 30 Jun 2020 01:30:05: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:30:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:30:06: 21000000 INFO @ Tue, 30 Jun 2020 01:30:08: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:30:12: 22000000 INFO @ Tue, 30 Jun 2020 01:30:15: 27000000 INFO @ Tue, 30 Jun 2020 01:30:18: 23000000 INFO @ Tue, 30 Jun 2020 01:30:21: 28000000 INFO @ Tue, 30 Jun 2020 01:30:24: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 01:30:24: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 01:30:24: #1 total tags in treatment: 28405530 INFO @ Tue, 30 Jun 2020 01:30:24: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:30:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:30:24: 24000000 INFO @ Tue, 30 Jun 2020 01:30:24: #1 tags after filtering in treatment: 28405530 INFO @ Tue, 30 Jun 2020 01:30:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:30:24: #1 finished! INFO @ Tue, 30 Jun 2020 01:30:24: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:30:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:30:26: #2 number of paired peaks: 3 WARNING @ Tue, 30 Jun 2020 01:30:26: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:30:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:30:30: 25000000 INFO @ Tue, 30 Jun 2020 01:30:36: 26000000 INFO @ Tue, 30 Jun 2020 01:30:42: 27000000 INFO @ Tue, 30 Jun 2020 01:30:48: 28000000 INFO @ Tue, 30 Jun 2020 01:30:51: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 01:30:51: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 01:30:51: #1 total tags in treatment: 28405530 INFO @ Tue, 30 Jun 2020 01:30:51: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:30:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:30:51: #1 tags after filtering in treatment: 28405530 INFO @ Tue, 30 Jun 2020 01:30:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:30:51: #1 finished! INFO @ Tue, 30 Jun 2020 01:30:51: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:30:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:30:53: #2 number of paired peaks: 3 WARNING @ Tue, 30 Jun 2020 01:30:53: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:30:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717028/SRX5717028.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。