Job ID = 14172263 SRX = SRX5681700 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 7912820 spots for SRR8895561/SRR8895561.sra Written 7912820 spots for SRR8895561/SRR8895561.sra Read 10309997 spots for SRR8895562/SRR8895562.sra Written 10309997 spots for SRR8895562/SRR8895562.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172889 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:35:17 18222817 reads; of these: 18222817 (100.00%) were paired; of these: 2196767 (12.06%) aligned concordantly 0 times 13352441 (73.27%) aligned concordantly exactly 1 time 2673609 (14.67%) aligned concordantly >1 times ---- 2196767 pairs aligned concordantly 0 times; of these: 858553 (39.08%) aligned discordantly 1 time ---- 1338214 pairs aligned 0 times concordantly or discordantly; of these: 2676428 mates make up the pairs; of these: 1818532 (67.95%) aligned 0 times 483979 (18.08%) aligned exactly 1 time 373917 (13.97%) aligned >1 times 95.01% overall alignment rate Time searching: 00:35:17 Overall time: 00:35:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 701548 / 16788346 = 0.0418 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:43:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:43:04: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:43:04: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:43:10: 1000000 INFO @ Sat, 11 Dec 2021 15:43:16: 2000000 INFO @ Sat, 11 Dec 2021 15:43:23: 3000000 INFO @ Sat, 11 Dec 2021 15:43:29: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:43:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:43:34: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:43:34: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:43:36: 5000000 INFO @ Sat, 11 Dec 2021 15:43:41: 1000000 INFO @ Sat, 11 Dec 2021 15:43:43: 6000000 INFO @ Sat, 11 Dec 2021 15:43:49: 2000000 INFO @ Sat, 11 Dec 2021 15:43:51: 7000000 INFO @ Sat, 11 Dec 2021 15:43:56: 3000000 INFO @ Sat, 11 Dec 2021 15:43:58: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:44:04: 4000000 INFO @ Sat, 11 Dec 2021 15:44:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:44:04: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:44:04: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:44:06: 9000000 INFO @ Sat, 11 Dec 2021 15:44:12: 5000000 INFO @ Sat, 11 Dec 2021 15:44:12: 1000000 INFO @ Sat, 11 Dec 2021 15:44:14: 10000000 INFO @ Sat, 11 Dec 2021 15:44:19: 6000000 INFO @ Sat, 11 Dec 2021 15:44:20: 2000000 INFO @ Sat, 11 Dec 2021 15:44:21: 11000000 INFO @ Sat, 11 Dec 2021 15:44:27: 7000000 INFO @ Sat, 11 Dec 2021 15:44:27: 3000000 INFO @ Sat, 11 Dec 2021 15:44:29: 12000000 INFO @ Sat, 11 Dec 2021 15:44:34: 8000000 INFO @ Sat, 11 Dec 2021 15:44:35: 4000000 INFO @ Sat, 11 Dec 2021 15:44:37: 13000000 INFO @ Sat, 11 Dec 2021 15:44:42: 9000000 INFO @ Sat, 11 Dec 2021 15:44:43: 5000000 INFO @ Sat, 11 Dec 2021 15:44:45: 14000000 INFO @ Sat, 11 Dec 2021 15:44:50: 10000000 INFO @ Sat, 11 Dec 2021 15:44:51: 6000000 INFO @ Sat, 11 Dec 2021 15:44:52: 15000000 INFO @ Sat, 11 Dec 2021 15:44:57: 11000000 INFO @ Sat, 11 Dec 2021 15:44:59: 7000000 INFO @ Sat, 11 Dec 2021 15:45:00: 16000000 INFO @ Sat, 11 Dec 2021 15:45:05: 12000000 INFO @ Sat, 11 Dec 2021 15:45:06: 8000000 INFO @ Sat, 11 Dec 2021 15:45:08: 17000000 INFO @ Sat, 11 Dec 2021 15:45:12: 13000000 INFO @ Sat, 11 Dec 2021 15:45:14: 9000000 INFO @ Sat, 11 Dec 2021 15:45:15: 18000000 INFO @ Sat, 11 Dec 2021 15:45:20: 14000000 INFO @ Sat, 11 Dec 2021 15:45:22: 10000000 INFO @ Sat, 11 Dec 2021 15:45:23: 19000000 INFO @ Sat, 11 Dec 2021 15:45:27: 15000000 INFO @ Sat, 11 Dec 2021 15:45:30: 11000000 INFO @ Sat, 11 Dec 2021 15:45:31: 20000000 INFO @ Sat, 11 Dec 2021 15:45:35: 16000000 INFO @ Sat, 11 Dec 2021 15:45:37: 12000000 INFO @ Sat, 11 Dec 2021 15:45:39: 21000000 INFO @ Sat, 11 Dec 2021 15:45:43: 17000000 INFO @ Sat, 11 Dec 2021 15:45:45: 13000000 INFO @ Sat, 11 Dec 2021 15:45:47: 22000000 INFO @ Sat, 11 Dec 2021 15:45:50: 18000000 INFO @ Sat, 11 Dec 2021 15:45:53: 14000000 INFO @ Sat, 11 Dec 2021 15:45:54: 23000000 INFO @ Sat, 11 Dec 2021 15:45:58: 19000000 INFO @ Sat, 11 Dec 2021 15:46:01: 15000000 INFO @ Sat, 11 Dec 2021 15:46:02: 24000000 INFO @ Sat, 11 Dec 2021 15:46:05: 20000000 INFO @ Sat, 11 Dec 2021 15:46:08: 16000000 INFO @ Sat, 11 Dec 2021 15:46:10: 25000000 INFO @ Sat, 11 Dec 2021 15:46:13: 21000000 INFO @ Sat, 11 Dec 2021 15:46:16: 17000000 INFO @ Sat, 11 Dec 2021 15:46:18: 26000000 INFO @ Sat, 11 Dec 2021 15:46:20: 22000000 INFO @ Sat, 11 Dec 2021 15:46:24: 18000000 INFO @ Sat, 11 Dec 2021 15:46:25: 27000000 INFO @ Sat, 11 Dec 2021 15:46:28: 23000000 INFO @ Sat, 11 Dec 2021 15:46:32: 19000000 INFO @ Sat, 11 Dec 2021 15:46:33: 28000000 INFO @ Sat, 11 Dec 2021 15:46:35: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 15:46:39: 20000000 INFO @ Sat, 11 Dec 2021 15:46:41: 29000000 INFO @ Sat, 11 Dec 2021 15:46:43: 25000000 INFO @ Sat, 11 Dec 2021 15:46:47: 21000000 INFO @ Sat, 11 Dec 2021 15:46:49: 30000000 INFO @ Sat, 11 Dec 2021 15:46:51: 26000000 INFO @ Sat, 11 Dec 2021 15:46:55: 22000000 INFO @ Sat, 11 Dec 2021 15:46:56: 31000000 INFO @ Sat, 11 Dec 2021 15:46:58: 27000000 INFO @ Sat, 11 Dec 2021 15:47:03: 23000000 INFO @ Sat, 11 Dec 2021 15:47:04: 32000000 INFO @ Sat, 11 Dec 2021 15:47:06: 28000000 INFO @ Sat, 11 Dec 2021 15:47:11: 24000000 INFO @ Sat, 11 Dec 2021 15:47:12: 33000000 INFO @ Sat, 11 Dec 2021 15:47:14: 29000000 INFO @ Sat, 11 Dec 2021 15:47:14: #1 tag size is determined as 100 bps INFO @ Sat, 11 Dec 2021 15:47:14: #1 tag size = 100 INFO @ Sat, 11 Dec 2021 15:47:14: #1 total tags in treatment: 15350431 INFO @ Sat, 11 Dec 2021 15:47:14: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:47:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:47:14: #1 tags after filtering in treatment: 13857333 INFO @ Sat, 11 Dec 2021 15:47:14: #1 Redundant rate of treatment: 0.10 INFO @ Sat, 11 Dec 2021 15:47:14: #1 finished! INFO @ Sat, 11 Dec 2021 15:47:14: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:47:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:47:15: #2 number of paired peaks: 3 WARNING @ Sat, 11 Dec 2021 15:47:15: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 15:47:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:47:18: 25000000 INFO @ Sat, 11 Dec 2021 15:47:21: 30000000 INFO @ Sat, 11 Dec 2021 15:47:26: 26000000 INFO @ Sat, 11 Dec 2021 15:47:29: 31000000 INFO @ Sat, 11 Dec 2021 15:47:34: 27000000 INFO @ Sat, 11 Dec 2021 15:47:36: 32000000 INFO @ Sat, 11 Dec 2021 15:47:41: 28000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 15:47:44: 33000000 INFO @ Sat, 11 Dec 2021 15:47:45: #1 tag size is determined as 100 bps INFO @ Sat, 11 Dec 2021 15:47:45: #1 tag size = 100 INFO @ Sat, 11 Dec 2021 15:47:45: #1 total tags in treatment: 15350431 INFO @ Sat, 11 Dec 2021 15:47:45: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:47:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:47:46: #1 tags after filtering in treatment: 13857333 INFO @ Sat, 11 Dec 2021 15:47:46: #1 Redundant rate of treatment: 0.10 INFO @ Sat, 11 Dec 2021 15:47:46: #1 finished! INFO @ Sat, 11 Dec 2021 15:47:46: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:47:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:47:46: #2 number of paired peaks: 3 WARNING @ Sat, 11 Dec 2021 15:47:46: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 15:47:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:47:49: 29000000 INFO @ Sat, 11 Dec 2021 15:47:56: 30000000 INFO @ Sat, 11 Dec 2021 15:48:03: 31000000 INFO @ Sat, 11 Dec 2021 15:48:10: 32000000 INFO @ Sat, 11 Dec 2021 15:48:17: 33000000 INFO @ Sat, 11 Dec 2021 15:48:18: #1 tag size is determined as 100 bps INFO @ Sat, 11 Dec 2021 15:48:18: #1 tag size = 100 INFO @ Sat, 11 Dec 2021 15:48:18: #1 total tags in treatment: 15350431 INFO @ Sat, 11 Dec 2021 15:48:18: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:48:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:48:18: #1 tags after filtering in treatment: 13857333 INFO @ Sat, 11 Dec 2021 15:48:18: #1 Redundant rate of treatment: 0.10 INFO @ Sat, 11 Dec 2021 15:48:18: #1 finished! INFO @ Sat, 11 Dec 2021 15:48:18: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:48:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:48:19: #2 number of paired peaks: 3 WARNING @ Sat, 11 Dec 2021 15:48:19: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 15:48:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling