Job ID = 10165802 SRX = SRX5681700 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 7912820 spots for SRR8895561/SRR8895561.sra Written 7912820 spots for SRR8895561/SRR8895561.sra Read 10309997 spots for SRR8895562/SRR8895562.sra Written 10309997 spots for SRR8895562/SRR8895562.sra fastq に変換しました。 bowtie でマッピング中... Your job 10166413 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:36:39 18222817 reads; of these: 18222817 (100.00%) were paired; of these: 2196767 (12.06%) aligned concordantly 0 times 13352441 (73.27%) aligned concordantly exactly 1 time 2673609 (14.67%) aligned concordantly >1 times ---- 2196767 pairs aligned concordantly 0 times; of these: 858553 (39.08%) aligned discordantly 1 time ---- 1338214 pairs aligned 0 times concordantly or discordantly; of these: 2676428 mates make up the pairs; of these: 1818532 (67.95%) aligned 0 times 483979 (18.08%) aligned exactly 1 time 373917 (13.97%) aligned >1 times 95.01% overall alignment rate Time searching: 00:36:40 Overall time: 00:36:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 701548 / 16788346 = 0.0418 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:43:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:43:01: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:43:01: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:43:08: 1000000 INFO @ Thu, 08 Oct 2020 20:43:15: 2000000 INFO @ Thu, 08 Oct 2020 20:43:22: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:43:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:43:27: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:43:27: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:43:29: 4000000 INFO @ Thu, 08 Oct 2020 20:43:35: 1000000 INFO @ Thu, 08 Oct 2020 20:43:37: 5000000 INFO @ Thu, 08 Oct 2020 20:43:42: 2000000 INFO @ Thu, 08 Oct 2020 20:43:45: 6000000 INFO @ Thu, 08 Oct 2020 20:43:50: 3000000 INFO @ Thu, 08 Oct 2020 20:43:53: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:43:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:43:57: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:43:57: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:43:58: 4000000 INFO @ Thu, 08 Oct 2020 20:44:01: 8000000 INFO @ Thu, 08 Oct 2020 20:44:05: 1000000 INFO @ Thu, 08 Oct 2020 20:44:06: 5000000 INFO @ Thu, 08 Oct 2020 20:44:09: 9000000 INFO @ Thu, 08 Oct 2020 20:44:13: 2000000 INFO @ Thu, 08 Oct 2020 20:44:14: 6000000 INFO @ Thu, 08 Oct 2020 20:44:17: 10000000 INFO @ Thu, 08 Oct 2020 20:44:21: 3000000 INFO @ Thu, 08 Oct 2020 20:44:23: 7000000 INFO @ Thu, 08 Oct 2020 20:44:25: 11000000 INFO @ Thu, 08 Oct 2020 20:44:29: 4000000 INFO @ Thu, 08 Oct 2020 20:44:31: 8000000 INFO @ Thu, 08 Oct 2020 20:44:33: 12000000 INFO @ Thu, 08 Oct 2020 20:44:37: 5000000 INFO @ Thu, 08 Oct 2020 20:44:39: 9000000 INFO @ Thu, 08 Oct 2020 20:44:41: 13000000 INFO @ Thu, 08 Oct 2020 20:44:45: 6000000 INFO @ Thu, 08 Oct 2020 20:44:47: 10000000 INFO @ Thu, 08 Oct 2020 20:44:49: 14000000 INFO @ Thu, 08 Oct 2020 20:44:52: 7000000 INFO @ Thu, 08 Oct 2020 20:44:55: 11000000 INFO @ Thu, 08 Oct 2020 20:44:57: 15000000 INFO @ Thu, 08 Oct 2020 20:45:00: 8000000 INFO @ Thu, 08 Oct 2020 20:45:03: 12000000 INFO @ Thu, 08 Oct 2020 20:45:05: 16000000 INFO @ Thu, 08 Oct 2020 20:45:08: 9000000 INFO @ Thu, 08 Oct 2020 20:45:11: 13000000 INFO @ Thu, 08 Oct 2020 20:45:13: 17000000 INFO @ Thu, 08 Oct 2020 20:45:16: 10000000 INFO @ Thu, 08 Oct 2020 20:45:19: 14000000 INFO @ Thu, 08 Oct 2020 20:45:21: 18000000 INFO @ Thu, 08 Oct 2020 20:45:24: 11000000 INFO @ Thu, 08 Oct 2020 20:45:27: 15000000 INFO @ Thu, 08 Oct 2020 20:45:29: 19000000 INFO @ Thu, 08 Oct 2020 20:45:32: 12000000 INFO @ Thu, 08 Oct 2020 20:45:35: 16000000 INFO @ Thu, 08 Oct 2020 20:45:37: 20000000 INFO @ Thu, 08 Oct 2020 20:45:40: 13000000 INFO @ Thu, 08 Oct 2020 20:45:43: 17000000 INFO @ Thu, 08 Oct 2020 20:45:45: 21000000 INFO @ Thu, 08 Oct 2020 20:45:48: 14000000 INFO @ Thu, 08 Oct 2020 20:45:51: 18000000 INFO @ Thu, 08 Oct 2020 20:45:54: 22000000 INFO @ Thu, 08 Oct 2020 20:45:56: 15000000 INFO @ Thu, 08 Oct 2020 20:45:59: 19000000 INFO @ Thu, 08 Oct 2020 20:46:02: 23000000 INFO @ Thu, 08 Oct 2020 20:46:04: 16000000 INFO @ Thu, 08 Oct 2020 20:46:07: 20000000 INFO @ Thu, 08 Oct 2020 20:46:10: 24000000 INFO @ Thu, 08 Oct 2020 20:46:12: 17000000 INFO @ Thu, 08 Oct 2020 20:46:15: 21000000 INFO @ Thu, 08 Oct 2020 20:46:18: 25000000 INFO @ Thu, 08 Oct 2020 20:46:20: 18000000 INFO @ Thu, 08 Oct 2020 20:46:23: 22000000 INFO @ Thu, 08 Oct 2020 20:46:26: 26000000 INFO @ Thu, 08 Oct 2020 20:46:28: 19000000 INFO @ Thu, 08 Oct 2020 20:46:31: 23000000 INFO @ Thu, 08 Oct 2020 20:46:34: 27000000 INFO @ Thu, 08 Oct 2020 20:46:36: 20000000 INFO @ Thu, 08 Oct 2020 20:46:38: 24000000 INFO @ Thu, 08 Oct 2020 20:46:42: 28000000 INFO @ Thu, 08 Oct 2020 20:46:44: 21000000 INFO @ Thu, 08 Oct 2020 20:46:46: 25000000 INFO @ Thu, 08 Oct 2020 20:46:50: 29000000 INFO @ Thu, 08 Oct 2020 20:46:52: 22000000 INFO @ Thu, 08 Oct 2020 20:46:54: 26000000 INFO @ Thu, 08 Oct 2020 20:46:58: 30000000 INFO @ Thu, 08 Oct 2020 20:47:00: 23000000 INFO @ Thu, 08 Oct 2020 20:47:02: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:47:06: 31000000 INFO @ Thu, 08 Oct 2020 20:47:07: 24000000 INFO @ Thu, 08 Oct 2020 20:47:10: 28000000 INFO @ Thu, 08 Oct 2020 20:47:14: 32000000 INFO @ Thu, 08 Oct 2020 20:47:15: 25000000 INFO @ Thu, 08 Oct 2020 20:47:18: 29000000 INFO @ Thu, 08 Oct 2020 20:47:22: 33000000 INFO @ Thu, 08 Oct 2020 20:47:23: 26000000 INFO @ Thu, 08 Oct 2020 20:47:23: #1 tag size is determined as 100 bps INFO @ Thu, 08 Oct 2020 20:47:23: #1 tag size = 100 INFO @ Thu, 08 Oct 2020 20:47:23: #1 total tags in treatment: 15350431 INFO @ Thu, 08 Oct 2020 20:47:23: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:47:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:47:24: #1 tags after filtering in treatment: 13857335 INFO @ Thu, 08 Oct 2020 20:47:24: #1 Redundant rate of treatment: 0.10 INFO @ Thu, 08 Oct 2020 20:47:24: #1 finished! INFO @ Thu, 08 Oct 2020 20:47:24: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:47:24: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:47:25: #2 number of paired peaks: 3 WARNING @ Thu, 08 Oct 2020 20:47:25: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:47:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:47:26: 30000000 INFO @ Thu, 08 Oct 2020 20:47:31: 27000000 INFO @ Thu, 08 Oct 2020 20:47:34: 31000000 INFO @ Thu, 08 Oct 2020 20:47:39: 28000000 INFO @ Thu, 08 Oct 2020 20:47:41: 32000000 INFO @ Thu, 08 Oct 2020 20:47:46: 29000000 INFO @ Thu, 08 Oct 2020 20:47:49: 33000000 INFO @ Thu, 08 Oct 2020 20:47:50: #1 tag size is determined as 100 bps INFO @ Thu, 08 Oct 2020 20:47:50: #1 tag size = 100 INFO @ Thu, 08 Oct 2020 20:47:50: #1 total tags in treatment: 15350431 INFO @ Thu, 08 Oct 2020 20:47:50: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:47:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:47:51: #1 tags after filtering in treatment: 13857335 INFO @ Thu, 08 Oct 2020 20:47:51: #1 Redundant rate of treatment: 0.10 INFO @ Thu, 08 Oct 2020 20:47:51: #1 finished! INFO @ Thu, 08 Oct 2020 20:47:51: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:47:51: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:47:52: #2 number of paired peaks: 3 WARNING @ Thu, 08 Oct 2020 20:47:52: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:47:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:47:54: 30000000 INFO @ Thu, 08 Oct 2020 20:48:00: 31000000 INFO @ Thu, 08 Oct 2020 20:48:07: 32000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 20:48:14: 33000000 INFO @ Thu, 08 Oct 2020 20:48:16: #1 tag size is determined as 100 bps INFO @ Thu, 08 Oct 2020 20:48:16: #1 tag size = 100 INFO @ Thu, 08 Oct 2020 20:48:16: #1 total tags in treatment: 15350431 INFO @ Thu, 08 Oct 2020 20:48:16: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:48:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:48:16: #1 tags after filtering in treatment: 13857335 INFO @ Thu, 08 Oct 2020 20:48:16: #1 Redundant rate of treatment: 0.10 INFO @ Thu, 08 Oct 2020 20:48:16: #1 finished! INFO @ Thu, 08 Oct 2020 20:48:16: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:48:16: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:48:17: #2 number of paired peaks: 3 WARNING @ Thu, 08 Oct 2020 20:48:17: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:48:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681700/SRX5681700.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling