Job ID = 1308924 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 50,603,172 reads read : 101,206,344 reads written : 50,603,172 reads 0-length : 50,603,172 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:26:40 50603172 reads; of these: 50603172 (100.00%) were unpaired; of these: 1656734 (3.27%) aligned 0 times 36020623 (71.18%) aligned exactly 1 time 12925815 (25.54%) aligned >1 times 96.73% overall alignment rate Time searching: 00:26:40 Overall time: 00:26:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7748796 / 48946438 = 0.1583 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 04 Jun 2019 00:02:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 04 Jun 2019 00:02:02: #1 read tag files... INFO @ Tue, 04 Jun 2019 00:02:02: #1 read treatment tags... INFO @ Tue, 04 Jun 2019 00:02:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 04 Jun 2019 00:02:02: #1 read tag files... INFO @ Tue, 04 Jun 2019 00:02:02: #1 read treatment tags... INFO @ Tue, 04 Jun 2019 00:02:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 04 Jun 2019 00:02:02: #1 read tag files... INFO @ Tue, 04 Jun 2019 00:02:02: #1 read treatment tags... INFO @ Tue, 04 Jun 2019 00:02:12: 1000000 INFO @ Tue, 04 Jun 2019 00:02:12: 1000000 INFO @ Tue, 04 Jun 2019 00:02:15: 1000000 INFO @ Tue, 04 Jun 2019 00:02:22: 2000000 INFO @ Tue, 04 Jun 2019 00:02:22: 2000000 INFO @ Tue, 04 Jun 2019 00:02:28: 2000000 INFO @ Tue, 04 Jun 2019 00:02:31: 3000000 INFO @ Tue, 04 Jun 2019 00:02:33: 3000000 INFO @ Tue, 04 Jun 2019 00:02:40: 3000000 INFO @ Tue, 04 Jun 2019 00:02:41: 4000000 INFO @ Tue, 04 Jun 2019 00:02:43: 4000000 INFO @ Tue, 04 Jun 2019 00:02:51: 5000000 INFO @ Tue, 04 Jun 2019 00:02:53: 5000000 INFO @ Tue, 04 Jun 2019 00:02:53: 4000000 INFO @ Tue, 04 Jun 2019 00:03:00: 6000000 INFO @ Tue, 04 Jun 2019 00:03:03: 6000000 INFO @ Tue, 04 Jun 2019 00:03:06: 5000000 INFO @ Tue, 04 Jun 2019 00:03:10: 7000000 INFO @ Tue, 04 Jun 2019 00:03:12: 7000000 INFO @ Tue, 04 Jun 2019 00:03:18: 6000000 INFO @ Tue, 04 Jun 2019 00:03:19: 8000000 INFO @ Tue, 04 Jun 2019 00:03:22: 8000000 INFO @ Tue, 04 Jun 2019 00:03:29: 9000000 INFO @ Tue, 04 Jun 2019 00:03:30: 7000000 INFO @ Tue, 04 Jun 2019 00:03:32: 9000000 INFO @ Tue, 04 Jun 2019 00:03:38: 10000000 INFO @ Tue, 04 Jun 2019 00:03:41: 8000000 INFO @ Tue, 04 Jun 2019 00:03:42: 10000000 INFO @ Tue, 04 Jun 2019 00:03:47: 11000000 INFO @ Tue, 04 Jun 2019 00:03:51: 11000000 INFO @ Tue, 04 Jun 2019 00:03:53: 9000000 INFO @ Tue, 04 Jun 2019 00:03:57: 12000000 INFO @ Tue, 04 Jun 2019 00:04:01: 12000000 INFO @ Tue, 04 Jun 2019 00:04:05: 10000000 INFO @ Tue, 04 Jun 2019 00:04:06: 13000000 INFO @ Tue, 04 Jun 2019 00:04:11: 13000000 INFO @ Tue, 04 Jun 2019 00:04:15: 14000000 INFO @ Tue, 04 Jun 2019 00:04:17: 11000000 INFO @ Tue, 04 Jun 2019 00:04:20: 14000000 INFO @ Tue, 04 Jun 2019 00:04:25: 15000000 INFO @ Tue, 04 Jun 2019 00:04:28: 12000000 INFO @ Tue, 04 Jun 2019 00:04:30: 15000000 INFO @ Tue, 04 Jun 2019 00:04:34: 16000000 INFO @ Tue, 04 Jun 2019 00:04:40: 16000000 INFO @ Tue, 04 Jun 2019 00:04:40: 13000000 INFO @ Tue, 04 Jun 2019 00:04:44: 17000000 INFO @ Tue, 04 Jun 2019 00:04:50: 17000000 INFO @ Tue, 04 Jun 2019 00:04:52: 14000000 INFO @ Tue, 04 Jun 2019 00:04:53: 18000000 INFO @ Tue, 04 Jun 2019 00:04:59: 18000000 INFO @ Tue, 04 Jun 2019 00:05:03: 19000000 INFO @ Tue, 04 Jun 2019 00:05:04: 15000000 INFO @ Tue, 04 Jun 2019 00:05:09: 19000000 INFO @ Tue, 04 Jun 2019 00:05:12: 20000000 INFO @ Tue, 04 Jun 2019 00:05:16: 16000000 INFO @ Tue, 04 Jun 2019 00:05:19: 20000000 INFO @ Tue, 04 Jun 2019 00:05:21: 21000000 INFO @ Tue, 04 Jun 2019 00:05:27: 17000000 INFO @ Tue, 04 Jun 2019 00:05:28: 21000000 INFO @ Tue, 04 Jun 2019 00:05:31: 22000000 INFO @ Tue, 04 Jun 2019 00:05:38: 22000000 INFO @ Tue, 04 Jun 2019 00:05:39: 18000000 INFO @ Tue, 04 Jun 2019 00:05:40: 23000000 INFO @ Tue, 04 Jun 2019 00:05:47: 23000000 INFO @ Tue, 04 Jun 2019 00:05:49: 24000000 INFO @ Tue, 04 Jun 2019 00:05:50: 19000000 INFO @ Tue, 04 Jun 2019 00:05:57: 24000000 INFO @ Tue, 04 Jun 2019 00:05:59: 25000000 INFO @ Tue, 04 Jun 2019 00:06:02: 20000000 INFO @ Tue, 04 Jun 2019 00:06:07: 25000000 INFO @ Tue, 04 Jun 2019 00:06:08: 26000000 INFO @ Tue, 04 Jun 2019 00:06:13: 21000000 INFO @ Tue, 04 Jun 2019 00:06:16: 26000000 INFO @ Tue, 04 Jun 2019 00:06:17: 27000000 INFO @ Tue, 04 Jun 2019 00:06:24: 22000000 INFO @ Tue, 04 Jun 2019 00:06:25: 28000000 INFO @ Tue, 04 Jun 2019 00:06:26: 27000000 INFO @ Tue, 04 Jun 2019 00:06:34: 29000000 INFO @ Tue, 04 Jun 2019 00:06:35: 23000000 INFO @ Tue, 04 Jun 2019 00:06:35: 28000000 INFO @ Tue, 04 Jun 2019 00:06:43: 30000000 INFO @ Tue, 04 Jun 2019 00:06:45: 29000000 INFO @ Tue, 04 Jun 2019 00:06:46: 24000000 INFO @ Tue, 04 Jun 2019 00:06:53: 31000000 INFO @ Tue, 04 Jun 2019 00:06:55: 30000000 INFO @ Tue, 04 Jun 2019 00:06:58: 25000000 INFO @ Tue, 04 Jun 2019 00:07:02: 32000000 INFO @ Tue, 04 Jun 2019 00:07:04: 31000000 INFO @ Tue, 04 Jun 2019 00:07:09: 26000000 INFO @ Tue, 04 Jun 2019 00:07:11: 33000000 INFO @ Tue, 04 Jun 2019 00:07:14: 32000000 INFO @ Tue, 04 Jun 2019 00:07:20: 27000000 INFO @ Tue, 04 Jun 2019 00:07:20: 34000000 INFO @ Tue, 04 Jun 2019 00:07:23: 33000000 INFO @ Tue, 04 Jun 2019 00:07:30: 35000000 INFO @ Tue, 04 Jun 2019 00:07:31: 28000000 INFO @ Tue, 04 Jun 2019 00:07:33: 34000000 INFO @ Tue, 04 Jun 2019 00:07:39: 36000000 INFO @ Tue, 04 Jun 2019 00:07:42: 35000000 INFO @ Tue, 04 Jun 2019 00:07:43: 29000000 INFO @ Tue, 04 Jun 2019 00:07:48: 37000000 INFO @ Tue, 04 Jun 2019 00:07:52: 36000000 INFO @ Tue, 04 Jun 2019 00:07:54: 30000000 INFO @ Tue, 04 Jun 2019 00:07:58: 38000000 INFO @ Tue, 04 Jun 2019 00:08:02: 37000000 INFO @ Tue, 04 Jun 2019 00:08:05: 31000000 INFO @ Tue, 04 Jun 2019 00:08:08: 39000000 INFO @ Tue, 04 Jun 2019 00:08:12: 38000000 INFO @ Tue, 04 Jun 2019 00:08:16: 32000000 INFO @ Tue, 04 Jun 2019 00:08:18: 40000000 INFO @ Tue, 04 Jun 2019 00:08:23: 39000000 INFO @ Tue, 04 Jun 2019 00:08:27: 41000000 INFO @ Tue, 04 Jun 2019 00:08:28: 33000000 INFO @ Tue, 04 Jun 2019 00:08:30: #1 tag size is determined as 62 bps INFO @ Tue, 04 Jun 2019 00:08:30: #1 tag size = 62 INFO @ Tue, 04 Jun 2019 00:08:30: #1 total tags in treatment: 41197642 INFO @ Tue, 04 Jun 2019 00:08:30: #1 user defined the maximum tags... INFO @ Tue, 04 Jun 2019 00:08:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 04 Jun 2019 00:08:31: #1 tags after filtering in treatment: 41197642 INFO @ Tue, 04 Jun 2019 00:08:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 04 Jun 2019 00:08:31: #1 finished! INFO @ Tue, 04 Jun 2019 00:08:31: #2 Build Peak Model... INFO @ Tue, 04 Jun 2019 00:08:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 04 Jun 2019 00:08:33: 40000000 INFO @ Tue, 04 Jun 2019 00:08:35: #2 number of paired peaks: 0 WARNING @ Tue, 04 Jun 2019 00:08:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 04 Jun 2019 00:08:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 04 Jun 2019 00:08:39: 34000000 INFO @ Tue, 04 Jun 2019 00:08:43: 41000000 INFO @ Tue, 04 Jun 2019 00:08:46: #1 tag size is determined as 62 bps INFO @ Tue, 04 Jun 2019 00:08:46: #1 tag size = 62 INFO @ Tue, 04 Jun 2019 00:08:46: #1 total tags in treatment: 41197642 INFO @ Tue, 04 Jun 2019 00:08:46: #1 user defined the maximum tags... INFO @ Tue, 04 Jun 2019 00:08:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 04 Jun 2019 00:08:47: #1 tags after filtering in treatment: 41197642 INFO @ Tue, 04 Jun 2019 00:08:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 04 Jun 2019 00:08:47: #1 finished! INFO @ Tue, 04 Jun 2019 00:08:47: #2 Build Peak Model... INFO @ Tue, 04 Jun 2019 00:08:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 04 Jun 2019 00:08:50: 35000000 INFO @ Tue, 04 Jun 2019 00:08:50: #2 number of paired peaks: 0 WARNING @ Tue, 04 Jun 2019 00:08:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 04 Jun 2019 00:08:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 04 Jun 2019 00:09:01: 36000000 INFO @ Tue, 04 Jun 2019 00:09:12: 37000000 INFO @ Tue, 04 Jun 2019 00:09:23: 38000000 INFO @ Tue, 04 Jun 2019 00:09:34: 39000000 INFO @ Tue, 04 Jun 2019 00:09:45: 40000000 INFO @ Tue, 04 Jun 2019 00:09:56: 41000000 INFO @ Tue, 04 Jun 2019 00:09:58: #1 tag size is determined as 62 bps INFO @ Tue, 04 Jun 2019 00:09:58: #1 tag size = 62 INFO @ Tue, 04 Jun 2019 00:09:58: #1 total tags in treatment: 41197642 INFO @ Tue, 04 Jun 2019 00:09:58: #1 user defined the maximum tags... INFO @ Tue, 04 Jun 2019 00:09:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 04 Jun 2019 00:09:59: #1 tags after filtering in treatment: 41197642 INFO @ Tue, 04 Jun 2019 00:09:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 04 Jun 2019 00:09:59: #1 finished! INFO @ Tue, 04 Jun 2019 00:09:59: #2 Build Peak Model... INFO @ Tue, 04 Jun 2019 00:09:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 04 Jun 2019 00:10:03: #2 number of paired peaks: 0 WARNING @ Tue, 04 Jun 2019 00:10:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 04 Jun 2019 00:10:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5661484/SRX5661484.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。