Job ID = 4178471 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 37,113,815 reads read : 37,113,815 reads written : 37,113,815 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:46 37113815 reads; of these: 37113815 (100.00%) were unpaired; of these: 826243 (2.23%) aligned 0 times 25443636 (68.56%) aligned exactly 1 time 10843936 (29.22%) aligned >1 times 97.77% overall alignment rate Time searching: 00:12:46 Overall time: 00:12:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 10649283 / 36287572 = 0.2935 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 05 Dec 2019 13:11:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:11:07: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:11:07: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:11:14: 1000000 INFO @ Thu, 05 Dec 2019 13:11:20: 2000000 INFO @ Thu, 05 Dec 2019 13:11:28: 3000000 INFO @ Thu, 05 Dec 2019 13:11:35: 4000000 INFO @ Thu, 05 Dec 2019 13:11:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:11:37: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:11:37: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:11:42: 5000000 INFO @ Thu, 05 Dec 2019 13:11:43: 1000000 INFO @ Thu, 05 Dec 2019 13:11:49: 6000000 INFO @ Thu, 05 Dec 2019 13:11:50: 2000000 INFO @ Thu, 05 Dec 2019 13:11:56: 3000000 INFO @ Thu, 05 Dec 2019 13:11:57: 7000000 INFO @ Thu, 05 Dec 2019 13:12:03: 4000000 INFO @ Thu, 05 Dec 2019 13:12:04: 8000000 BedGraph に変換中... INFO @ Thu, 05 Dec 2019 13:12:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:12:07: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:12:07: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:12:09: 5000000 INFO @ Thu, 05 Dec 2019 13:12:12: 9000000 INFO @ Thu, 05 Dec 2019 13:12:15: 1000000 INFO @ Thu, 05 Dec 2019 13:12:16: 6000000 INFO @ Thu, 05 Dec 2019 13:12:20: 10000000 INFO @ Thu, 05 Dec 2019 13:12:22: 7000000 INFO @ Thu, 05 Dec 2019 13:12:23: 2000000 INFO @ Thu, 05 Dec 2019 13:12:28: 11000000 INFO @ Thu, 05 Dec 2019 13:12:29: 8000000 INFO @ Thu, 05 Dec 2019 13:12:31: 3000000 INFO @ Thu, 05 Dec 2019 13:12:35: 9000000 INFO @ Thu, 05 Dec 2019 13:12:36: 12000000 INFO @ Thu, 05 Dec 2019 13:12:39: 4000000 INFO @ Thu, 05 Dec 2019 13:12:42: 10000000 INFO @ Thu, 05 Dec 2019 13:12:45: 13000000 INFO @ Thu, 05 Dec 2019 13:12:47: 5000000 INFO @ Thu, 05 Dec 2019 13:12:48: 11000000 INFO @ Thu, 05 Dec 2019 13:12:54: 14000000 INFO @ Thu, 05 Dec 2019 13:12:55: 12000000 INFO @ Thu, 05 Dec 2019 13:12:56: 6000000 INFO @ Thu, 05 Dec 2019 13:13:02: 13000000 INFO @ Thu, 05 Dec 2019 13:13:03: 15000000 INFO @ Thu, 05 Dec 2019 13:13:05: 7000000 INFO @ Thu, 05 Dec 2019 13:13:09: 14000000 INFO @ Thu, 05 Dec 2019 13:13:12: 16000000 INFO @ Thu, 05 Dec 2019 13:13:13: 8000000 INFO @ Thu, 05 Dec 2019 13:13:17: 15000000 INFO @ Thu, 05 Dec 2019 13:13:20: 17000000 INFO @ Thu, 05 Dec 2019 13:13:21: 9000000 INFO @ Thu, 05 Dec 2019 13:13:25: 16000000 INFO @ Thu, 05 Dec 2019 13:13:29: 18000000 INFO @ Thu, 05 Dec 2019 13:13:30: 10000000 INFO @ Thu, 05 Dec 2019 13:13:32: 17000000 INFO @ Thu, 05 Dec 2019 13:13:37: 19000000 INFO @ Thu, 05 Dec 2019 13:13:38: 11000000 INFO @ Thu, 05 Dec 2019 13:13:40: 18000000 INFO @ Thu, 05 Dec 2019 13:13:45: 20000000 INFO @ Thu, 05 Dec 2019 13:13:46: 12000000 INFO @ Thu, 05 Dec 2019 13:13:47: 19000000 INFO @ Thu, 05 Dec 2019 13:13:53: 21000000 INFO @ Thu, 05 Dec 2019 13:13:55: 13000000 INFO @ Thu, 05 Dec 2019 13:13:55: 20000000 INFO @ Thu, 05 Dec 2019 13:14:01: 22000000 INFO @ Thu, 05 Dec 2019 13:14:02: 21000000 INFO @ Thu, 05 Dec 2019 13:14:03: 14000000 INFO @ Thu, 05 Dec 2019 13:14:08: 23000000 INFO @ Thu, 05 Dec 2019 13:14:09: 22000000 INFO @ Thu, 05 Dec 2019 13:14:11: 15000000 INFO @ Thu, 05 Dec 2019 13:14:16: 23000000 INFO @ Thu, 05 Dec 2019 13:14:16: 24000000 INFO @ Thu, 05 Dec 2019 13:14:19: 16000000 INFO @ Thu, 05 Dec 2019 13:14:22: 24000000 INFO @ Thu, 05 Dec 2019 13:14:23: 25000000 INFO @ Thu, 05 Dec 2019 13:14:28: 17000000 INFO @ Thu, 05 Dec 2019 13:14:28: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 13:14:28: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 13:14:28: #1 total tags in treatment: 25638289 INFO @ Thu, 05 Dec 2019 13:14:28: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:14:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:14:29: #1 tags after filtering in treatment: 25638289 INFO @ Thu, 05 Dec 2019 13:14:29: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:14:29: #1 finished! INFO @ Thu, 05 Dec 2019 13:14:29: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:14:29: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:14:29: 25000000 INFO @ Thu, 05 Dec 2019 13:14:30: #2 number of paired peaks: 2 WARNING @ Thu, 05 Dec 2019 13:14:30: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:14:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 13:14:33: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 13:14:33: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 13:14:33: #1 total tags in treatment: 25638289 INFO @ Thu, 05 Dec 2019 13:14:33: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:14:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:14:34: #1 tags after filtering in treatment: 25638289 INFO @ Thu, 05 Dec 2019 13:14:34: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:14:34: #1 finished! INFO @ Thu, 05 Dec 2019 13:14:34: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:14:34: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:14:35: 18000000 INFO @ Thu, 05 Dec 2019 13:14:35: #2 number of paired peaks: 2 WARNING @ Thu, 05 Dec 2019 13:14:35: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:14:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 13:14:41: 19000000 INFO @ Thu, 05 Dec 2019 13:14:48: 20000000 INFO @ Thu, 05 Dec 2019 13:14:55: 21000000 INFO @ Thu, 05 Dec 2019 13:15:02: 22000000 INFO @ Thu, 05 Dec 2019 13:15:09: 23000000 INFO @ Thu, 05 Dec 2019 13:15:16: 24000000 INFO @ Thu, 05 Dec 2019 13:15:23: 25000000 INFO @ Thu, 05 Dec 2019 13:15:27: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 13:15:27: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 13:15:27: #1 total tags in treatment: 25638289 INFO @ Thu, 05 Dec 2019 13:15:27: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:15:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:15:28: #1 tags after filtering in treatment: 25638289 INFO @ Thu, 05 Dec 2019 13:15:28: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:15:28: #1 finished! INFO @ Thu, 05 Dec 2019 13:15:28: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:15:28: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:15:29: #2 number of paired peaks: 2 WARNING @ Thu, 05 Dec 2019 13:15:29: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:15:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5535371/SRX5535371.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。