Job ID = 2590931 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 29,516,345 reads read : 29,516,345 reads written : 29,516,345 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:36 29516345 reads; of these: 29516345 (100.00%) were unpaired; of these: 1813892 (6.15%) aligned 0 times 18434396 (62.45%) aligned exactly 1 time 9268057 (31.40%) aligned >1 times 93.85% overall alignment rate Time searching: 00:12:36 Overall time: 00:12:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3722002 / 27702453 = 0.1344 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 13 Aug 2019 00:27:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 13 Aug 2019 00:27:10: #1 read tag files... INFO @ Tue, 13 Aug 2019 00:27:10: #1 read treatment tags... INFO @ Tue, 13 Aug 2019 00:27:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 13 Aug 2019 00:27:11: #1 read tag files... INFO @ Tue, 13 Aug 2019 00:27:11: #1 read treatment tags... INFO @ Tue, 13 Aug 2019 00:27:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 13 Aug 2019 00:27:12: #1 read tag files... INFO @ Tue, 13 Aug 2019 00:27:12: #1 read treatment tags... INFO @ Tue, 13 Aug 2019 00:27:18: 1000000 INFO @ Tue, 13 Aug 2019 00:27:18: 1000000 INFO @ Tue, 13 Aug 2019 00:27:20: 1000000 INFO @ Tue, 13 Aug 2019 00:27:26: 2000000 INFO @ Tue, 13 Aug 2019 00:27:26: 2000000 INFO @ Tue, 13 Aug 2019 00:27:28: 2000000 INFO @ Tue, 13 Aug 2019 00:27:33: 3000000 INFO @ Tue, 13 Aug 2019 00:27:34: 3000000 INFO @ Tue, 13 Aug 2019 00:27:36: 3000000 INFO @ Tue, 13 Aug 2019 00:27:40: 4000000 INFO @ Tue, 13 Aug 2019 00:27:43: 4000000 INFO @ Tue, 13 Aug 2019 00:27:44: 4000000 INFO @ Tue, 13 Aug 2019 00:27:48: 5000000 INFO @ Tue, 13 Aug 2019 00:27:51: 5000000 INFO @ Tue, 13 Aug 2019 00:27:52: 5000000 INFO @ Tue, 13 Aug 2019 00:27:55: 6000000 INFO @ Tue, 13 Aug 2019 00:27:59: 6000000 INFO @ Tue, 13 Aug 2019 00:28:01: 6000000 INFO @ Tue, 13 Aug 2019 00:28:03: 7000000 INFO @ Tue, 13 Aug 2019 00:28:07: 7000000 INFO @ Tue, 13 Aug 2019 00:28:09: 7000000 INFO @ Tue, 13 Aug 2019 00:28:10: 8000000 INFO @ Tue, 13 Aug 2019 00:28:16: 8000000 INFO @ Tue, 13 Aug 2019 00:28:17: 8000000 INFO @ Tue, 13 Aug 2019 00:28:17: 9000000 INFO @ Tue, 13 Aug 2019 00:28:24: 9000000 INFO @ Tue, 13 Aug 2019 00:28:25: 10000000 INFO @ Tue, 13 Aug 2019 00:28:25: 9000000 INFO @ Tue, 13 Aug 2019 00:28:32: 11000000 INFO @ Tue, 13 Aug 2019 00:28:32: 10000000 INFO @ Tue, 13 Aug 2019 00:28:33: 10000000 INFO @ Tue, 13 Aug 2019 00:28:39: 12000000 INFO @ Tue, 13 Aug 2019 00:28:41: 11000000 INFO @ Tue, 13 Aug 2019 00:28:41: 11000000 INFO @ Tue, 13 Aug 2019 00:28:46: 13000000 INFO @ Tue, 13 Aug 2019 00:28:49: 12000000 INFO @ Tue, 13 Aug 2019 00:28:50: 12000000 INFO @ Tue, 13 Aug 2019 00:28:54: 14000000 INFO @ Tue, 13 Aug 2019 00:28:57: 13000000 INFO @ Tue, 13 Aug 2019 00:28:59: 13000000 INFO @ Tue, 13 Aug 2019 00:29:01: 15000000 INFO @ Tue, 13 Aug 2019 00:29:05: 14000000 INFO @ Tue, 13 Aug 2019 00:29:07: 14000000 INFO @ Tue, 13 Aug 2019 00:29:08: 16000000 INFO @ Tue, 13 Aug 2019 00:29:13: 15000000 INFO @ Tue, 13 Aug 2019 00:29:15: 15000000 INFO @ Tue, 13 Aug 2019 00:29:16: 17000000 INFO @ Tue, 13 Aug 2019 00:29:21: 16000000 INFO @ Tue, 13 Aug 2019 00:29:23: 18000000 INFO @ Tue, 13 Aug 2019 00:29:23: 16000000 INFO @ Tue, 13 Aug 2019 00:29:30: 17000000 INFO @ Tue, 13 Aug 2019 00:29:30: 19000000 INFO @ Tue, 13 Aug 2019 00:29:31: 17000000 INFO @ Tue, 13 Aug 2019 00:29:37: 20000000 INFO @ Tue, 13 Aug 2019 00:29:38: 18000000 INFO @ Tue, 13 Aug 2019 00:29:40: 18000000 INFO @ Tue, 13 Aug 2019 00:29:45: 21000000 INFO @ Tue, 13 Aug 2019 00:29:47: 19000000 INFO @ Tue, 13 Aug 2019 00:29:52: 19000000 INFO @ Tue, 13 Aug 2019 00:29:52: 22000000 INFO @ Tue, 13 Aug 2019 00:29:59: 20000000 INFO @ Tue, 13 Aug 2019 00:29:59: 23000000 INFO @ Tue, 13 Aug 2019 00:30:03: 20000000 INFO @ Tue, 13 Aug 2019 00:30:06: #1 tag size is determined as 50 bps INFO @ Tue, 13 Aug 2019 00:30:06: #1 tag size = 50 INFO @ Tue, 13 Aug 2019 00:30:06: #1 total tags in treatment: 23980451 INFO @ Tue, 13 Aug 2019 00:30:06: #1 user defined the maximum tags... INFO @ Tue, 13 Aug 2019 00:30:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 13 Aug 2019 00:30:07: #1 tags after filtering in treatment: 23980451 INFO @ Tue, 13 Aug 2019 00:30:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 13 Aug 2019 00:30:07: #1 finished! INFO @ Tue, 13 Aug 2019 00:30:07: #2 Build Peak Model... INFO @ Tue, 13 Aug 2019 00:30:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 13 Aug 2019 00:30:09: #2 number of paired peaks: 94 WARNING @ Tue, 13 Aug 2019 00:30:09: Too few paired peaks (94) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 13 Aug 2019 00:30:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 13 Aug 2019 00:30:09: 21000000 INFO @ Tue, 13 Aug 2019 00:30:13: 21000000 INFO @ Tue, 13 Aug 2019 00:30:17: 22000000 INFO @ Tue, 13 Aug 2019 00:30:23: 22000000 INFO @ Tue, 13 Aug 2019 00:30:26: 23000000 INFO @ Tue, 13 Aug 2019 00:30:32: 23000000 INFO @ Tue, 13 Aug 2019 00:30:34: #1 tag size is determined as 50 bps INFO @ Tue, 13 Aug 2019 00:30:34: #1 tag size = 50 INFO @ Tue, 13 Aug 2019 00:30:34: #1 total tags in treatment: 23980451 INFO @ Tue, 13 Aug 2019 00:30:34: #1 user defined the maximum tags... INFO @ Tue, 13 Aug 2019 00:30:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 13 Aug 2019 00:30:35: #1 tags after filtering in treatment: 23980451 INFO @ Tue, 13 Aug 2019 00:30:35: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 13 Aug 2019 00:30:35: #1 finished! INFO @ Tue, 13 Aug 2019 00:30:35: #2 Build Peak Model... INFO @ Tue, 13 Aug 2019 00:30:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 13 Aug 2019 00:30:37: #2 number of paired peaks: 94 WARNING @ Tue, 13 Aug 2019 00:30:37: Too few paired peaks (94) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 13 Aug 2019 00:30:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 13 Aug 2019 00:30:42: #1 tag size is determined as 50 bps INFO @ Tue, 13 Aug 2019 00:30:42: #1 tag size = 50 INFO @ Tue, 13 Aug 2019 00:30:42: #1 total tags in treatment: 23980451 INFO @ Tue, 13 Aug 2019 00:30:42: #1 user defined the maximum tags... INFO @ Tue, 13 Aug 2019 00:30:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 13 Aug 2019 00:30:42: #1 tags after filtering in treatment: 23980451 INFO @ Tue, 13 Aug 2019 00:30:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 13 Aug 2019 00:30:42: #1 finished! INFO @ Tue, 13 Aug 2019 00:30:42: #2 Build Peak Model... INFO @ Tue, 13 Aug 2019 00:30:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 13 Aug 2019 00:30:44: #2 number of paired peaks: 94 WARNING @ Tue, 13 Aug 2019 00:30:44: Too few paired peaks (94) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 13 Aug 2019 00:30:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431043/SRX5431043.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。