Job ID = 6528361 SRX = SRX5431036 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:44:25 prefetch.2.10.7: 1) Downloading 'SRR8632335'... 2020-06-29T14:44:25 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:46:07 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:46:08 prefetch.2.10.7: 'SRR8632335' is valid 2020-06-29T14:46:08 prefetch.2.10.7: 1) 'SRR8632335' was downloaded successfully Read 18875551 spots for SRR8632335/SRR8632335.sra Written 18875551 spots for SRR8632335/SRR8632335.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:34 18875551 reads; of these: 18875551 (100.00%) were unpaired; of these: 1379243 (7.31%) aligned 0 times 13233467 (70.11%) aligned exactly 1 time 4262841 (22.58%) aligned >1 times 92.69% overall alignment rate Time searching: 00:05:34 Overall time: 00:05:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1369771 / 17496308 = 0.0783 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:01:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:01:03: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:01:03: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:01:10: 1000000 INFO @ Tue, 30 Jun 2020 00:01:17: 2000000 INFO @ Tue, 30 Jun 2020 00:01:24: 3000000 INFO @ Tue, 30 Jun 2020 00:01:31: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:01:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:01:33: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:01:33: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:01:38: 5000000 INFO @ Tue, 30 Jun 2020 00:01:39: 1000000 INFO @ Tue, 30 Jun 2020 00:01:45: 6000000 INFO @ Tue, 30 Jun 2020 00:01:46: 2000000 INFO @ Tue, 30 Jun 2020 00:01:53: 3000000 INFO @ Tue, 30 Jun 2020 00:01:53: 7000000 INFO @ Tue, 30 Jun 2020 00:02:00: 4000000 INFO @ Tue, 30 Jun 2020 00:02:01: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:02:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:02:03: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:02:03: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:02:06: 5000000 INFO @ Tue, 30 Jun 2020 00:02:08: 9000000 INFO @ Tue, 30 Jun 2020 00:02:10: 1000000 INFO @ Tue, 30 Jun 2020 00:02:13: 6000000 INFO @ Tue, 30 Jun 2020 00:02:16: 10000000 INFO @ Tue, 30 Jun 2020 00:02:17: 2000000 INFO @ Tue, 30 Jun 2020 00:02:20: 7000000 INFO @ Tue, 30 Jun 2020 00:02:24: 11000000 INFO @ Tue, 30 Jun 2020 00:02:24: 3000000 INFO @ Tue, 30 Jun 2020 00:02:27: 8000000 INFO @ Tue, 30 Jun 2020 00:02:31: 4000000 INFO @ Tue, 30 Jun 2020 00:02:31: 12000000 INFO @ Tue, 30 Jun 2020 00:02:34: 9000000 INFO @ Tue, 30 Jun 2020 00:02:38: 5000000 INFO @ Tue, 30 Jun 2020 00:02:39: 13000000 INFO @ Tue, 30 Jun 2020 00:02:40: 10000000 INFO @ Tue, 30 Jun 2020 00:02:45: 6000000 INFO @ Tue, 30 Jun 2020 00:02:47: 14000000 INFO @ Tue, 30 Jun 2020 00:02:47: 11000000 INFO @ Tue, 30 Jun 2020 00:02:52: 7000000 INFO @ Tue, 30 Jun 2020 00:02:54: 12000000 INFO @ Tue, 30 Jun 2020 00:02:54: 15000000 INFO @ Tue, 30 Jun 2020 00:02:59: 8000000 INFO @ Tue, 30 Jun 2020 00:03:01: 13000000 INFO @ Tue, 30 Jun 2020 00:03:02: 16000000 INFO @ Tue, 30 Jun 2020 00:03:03: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:03:03: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:03:03: #1 total tags in treatment: 16126537 INFO @ Tue, 30 Jun 2020 00:03:03: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:03:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:03:03: #1 tags after filtering in treatment: 16126537 INFO @ Tue, 30 Jun 2020 00:03:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:03:03: #1 finished! INFO @ Tue, 30 Jun 2020 00:03:03: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:03:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:03:04: #2 number of paired peaks: 97 WARNING @ Tue, 30 Jun 2020 00:03:04: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:03:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:03:06: 9000000 INFO @ Tue, 30 Jun 2020 00:03:08: 14000000 INFO @ Tue, 30 Jun 2020 00:03:13: 10000000 INFO @ Tue, 30 Jun 2020 00:03:14: 15000000 INFO @ Tue, 30 Jun 2020 00:03:20: 11000000 INFO @ Tue, 30 Jun 2020 00:03:21: 16000000 INFO @ Tue, 30 Jun 2020 00:03:21: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:03:21: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:03:21: #1 total tags in treatment: 16126537 INFO @ Tue, 30 Jun 2020 00:03:21: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:03:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:03:22: #1 tags after filtering in treatment: 16126537 INFO @ Tue, 30 Jun 2020 00:03:22: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:03:22: #1 finished! INFO @ Tue, 30 Jun 2020 00:03:22: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:03:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:03:23: #2 number of paired peaks: 97 WARNING @ Tue, 30 Jun 2020 00:03:23: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:03:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:03:26: 12000000 INFO @ Tue, 30 Jun 2020 00:03:33: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:03:39: 14000000 INFO @ Tue, 30 Jun 2020 00:03:45: 15000000 INFO @ Tue, 30 Jun 2020 00:03:51: 16000000 INFO @ Tue, 30 Jun 2020 00:03:51: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:03:51: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:03:51: #1 total tags in treatment: 16126537 INFO @ Tue, 30 Jun 2020 00:03:51: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:03:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:03:52: #1 tags after filtering in treatment: 16126537 INFO @ Tue, 30 Jun 2020 00:03:52: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:03:52: #1 finished! INFO @ Tue, 30 Jun 2020 00:03:52: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:03:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:03:53: #2 number of paired peaks: 97 WARNING @ Tue, 30 Jun 2020 00:03:53: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:03:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431036/SRX5431036.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。