Job ID = 6528360 SRX = SRX5431035 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:05:35 prefetch.2.10.7: 1) Downloading 'SRR8632334'... 2020-06-29T15:05:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:08:05 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:08:05 prefetch.2.10.7: 1) 'SRR8632334' was downloaded successfully Read 20413076 spots for SRR8632334/SRR8632334.sra Written 20413076 spots for SRR8632334/SRR8632334.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:45 20413076 reads; of these: 20413076 (100.00%) were unpaired; of these: 1506023 (7.38%) aligned 0 times 13637640 (66.81%) aligned exactly 1 time 5269413 (25.81%) aligned >1 times 92.62% overall alignment rate Time searching: 00:06:45 Overall time: 00:06:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1988755 / 18907053 = 0.1052 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:27:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:27:51: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:27:51: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:27:57: 1000000 INFO @ Tue, 30 Jun 2020 00:28:03: 2000000 INFO @ Tue, 30 Jun 2020 00:28:09: 3000000 INFO @ Tue, 30 Jun 2020 00:28:16: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:28:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:28:20: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:28:20: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:28:22: 5000000 INFO @ Tue, 30 Jun 2020 00:28:27: 1000000 INFO @ Tue, 30 Jun 2020 00:28:28: 6000000 INFO @ Tue, 30 Jun 2020 00:28:34: 2000000 INFO @ Tue, 30 Jun 2020 00:28:35: 7000000 INFO @ Tue, 30 Jun 2020 00:28:40: 3000000 INFO @ Tue, 30 Jun 2020 00:28:41: 8000000 INFO @ Tue, 30 Jun 2020 00:28:47: 4000000 INFO @ Tue, 30 Jun 2020 00:28:48: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:28:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:28:51: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:28:51: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:28:53: 5000000 INFO @ Tue, 30 Jun 2020 00:28:54: 10000000 INFO @ Tue, 30 Jun 2020 00:28:57: 1000000 INFO @ Tue, 30 Jun 2020 00:29:00: 6000000 INFO @ Tue, 30 Jun 2020 00:29:01: 11000000 INFO @ Tue, 30 Jun 2020 00:29:04: 2000000 INFO @ Tue, 30 Jun 2020 00:29:07: 7000000 INFO @ Tue, 30 Jun 2020 00:29:08: 12000000 INFO @ Tue, 30 Jun 2020 00:29:11: 3000000 INFO @ Tue, 30 Jun 2020 00:29:13: 8000000 INFO @ Tue, 30 Jun 2020 00:29:15: 13000000 INFO @ Tue, 30 Jun 2020 00:29:18: 4000000 INFO @ Tue, 30 Jun 2020 00:29:20: 9000000 INFO @ Tue, 30 Jun 2020 00:29:21: 14000000 INFO @ Tue, 30 Jun 2020 00:29:25: 5000000 INFO @ Tue, 30 Jun 2020 00:29:27: 10000000 INFO @ Tue, 30 Jun 2020 00:29:28: 15000000 INFO @ Tue, 30 Jun 2020 00:29:31: 6000000 INFO @ Tue, 30 Jun 2020 00:29:33: 11000000 INFO @ Tue, 30 Jun 2020 00:29:35: 16000000 INFO @ Tue, 30 Jun 2020 00:29:38: 7000000 INFO @ Tue, 30 Jun 2020 00:29:40: 12000000 INFO @ Tue, 30 Jun 2020 00:29:41: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:29:41: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:29:41: #1 total tags in treatment: 16918298 INFO @ Tue, 30 Jun 2020 00:29:41: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:29:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:29:42: #1 tags after filtering in treatment: 16918298 INFO @ Tue, 30 Jun 2020 00:29:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:29:42: #1 finished! INFO @ Tue, 30 Jun 2020 00:29:42: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:29:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:29:43: #2 number of paired peaks: 93 WARNING @ Tue, 30 Jun 2020 00:29:43: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:29:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:29:45: 8000000 INFO @ Tue, 30 Jun 2020 00:29:46: 13000000 INFO @ Tue, 30 Jun 2020 00:29:51: 9000000 INFO @ Tue, 30 Jun 2020 00:29:53: 14000000 INFO @ Tue, 30 Jun 2020 00:29:57: 10000000 INFO @ Tue, 30 Jun 2020 00:29:59: 15000000 INFO @ Tue, 30 Jun 2020 00:30:04: 11000000 INFO @ Tue, 30 Jun 2020 00:30:05: 16000000 INFO @ Tue, 30 Jun 2020 00:30:10: 12000000 INFO @ Tue, 30 Jun 2020 00:30:11: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:30:11: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:30:11: #1 total tags in treatment: 16918298 INFO @ Tue, 30 Jun 2020 00:30:11: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:30:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:30:12: #1 tags after filtering in treatment: 16918298 INFO @ Tue, 30 Jun 2020 00:30:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:30:12: #1 finished! INFO @ Tue, 30 Jun 2020 00:30:12: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:30:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:30:13: #2 number of paired peaks: 93 WARNING @ Tue, 30 Jun 2020 00:30:13: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:30:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:30:16: 13000000 INFO @ Tue, 30 Jun 2020 00:30:22: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:30:27: 15000000 INFO @ Tue, 30 Jun 2020 00:30:33: 16000000 INFO @ Tue, 30 Jun 2020 00:30:39: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:30:39: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:30:39: #1 total tags in treatment: 16918298 INFO @ Tue, 30 Jun 2020 00:30:39: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:30:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:30:39: #1 tags after filtering in treatment: 16918298 INFO @ Tue, 30 Jun 2020 00:30:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:30:39: #1 finished! INFO @ Tue, 30 Jun 2020 00:30:39: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:30:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:30:40: #2 number of paired peaks: 93 WARNING @ Tue, 30 Jun 2020 00:30:40: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:30:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431035/SRX5431035.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。