Job ID = 5720840 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 48,654,173 reads read : 48,654,173 reads written : 48,654,173 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:47 48654173 reads; of these: 48654173 (100.00%) were unpaired; of these: 3275272 (6.73%) aligned 0 times 31292627 (64.32%) aligned exactly 1 time 14086274 (28.95%) aligned >1 times 93.27% overall alignment rate Time searching: 00:17:47 Overall time: 00:17:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 11820062 / 45378901 = 0.2605 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 02:57:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 02:57:30: #1 read tag files... INFO @ Thu, 16 Apr 2020 02:57:30: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 02:57:36: 1000000 INFO @ Thu, 16 Apr 2020 02:57:42: 2000000 INFO @ Thu, 16 Apr 2020 02:57:47: 3000000 INFO @ Thu, 16 Apr 2020 02:57:52: 4000000 INFO @ Thu, 16 Apr 2020 02:57:58: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 02:58:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 02:58:01: #1 read tag files... INFO @ Thu, 16 Apr 2020 02:58:01: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 02:58:03: 6000000 INFO @ Thu, 16 Apr 2020 02:58:06: 1000000 INFO @ Thu, 16 Apr 2020 02:58:08: 7000000 INFO @ Thu, 16 Apr 2020 02:58:12: 2000000 INFO @ Thu, 16 Apr 2020 02:58:14: 8000000 INFO @ Thu, 16 Apr 2020 02:58:17: 3000000 INFO @ Thu, 16 Apr 2020 02:58:19: 9000000 INFO @ Thu, 16 Apr 2020 02:58:22: 4000000 INFO @ Thu, 16 Apr 2020 02:58:25: 10000000 INFO @ Thu, 16 Apr 2020 02:58:28: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 02:58:30: 11000000 INFO @ Thu, 16 Apr 2020 02:58:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 02:58:31: #1 read tag files... INFO @ Thu, 16 Apr 2020 02:58:31: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 02:58:33: 6000000 INFO @ Thu, 16 Apr 2020 02:58:36: 12000000 INFO @ Thu, 16 Apr 2020 02:58:37: 1000000 INFO @ Thu, 16 Apr 2020 02:58:39: 7000000 INFO @ Thu, 16 Apr 2020 02:58:42: 13000000 INFO @ Thu, 16 Apr 2020 02:58:44: 2000000 INFO @ Thu, 16 Apr 2020 02:58:45: 8000000 INFO @ Thu, 16 Apr 2020 02:58:49: 14000000 INFO @ Thu, 16 Apr 2020 02:58:50: 3000000 INFO @ Thu, 16 Apr 2020 02:58:51: 9000000 INFO @ Thu, 16 Apr 2020 02:58:55: 15000000 INFO @ Thu, 16 Apr 2020 02:58:56: 4000000 INFO @ Thu, 16 Apr 2020 02:58:57: 10000000 INFO @ Thu, 16 Apr 2020 02:59:01: 16000000 INFO @ Thu, 16 Apr 2020 02:59:02: 5000000 INFO @ Thu, 16 Apr 2020 02:59:03: 11000000 INFO @ Thu, 16 Apr 2020 02:59:07: 17000000 INFO @ Thu, 16 Apr 2020 02:59:09: 12000000 INFO @ Thu, 16 Apr 2020 02:59:09: 6000000 INFO @ Thu, 16 Apr 2020 02:59:13: 18000000 INFO @ Thu, 16 Apr 2020 02:59:14: 13000000 INFO @ Thu, 16 Apr 2020 02:59:16: 7000000 INFO @ Thu, 16 Apr 2020 02:59:19: 19000000 INFO @ Thu, 16 Apr 2020 02:59:20: 14000000 INFO @ Thu, 16 Apr 2020 02:59:22: 8000000 INFO @ Thu, 16 Apr 2020 02:59:25: 20000000 INFO @ Thu, 16 Apr 2020 02:59:26: 15000000 INFO @ Thu, 16 Apr 2020 02:59:29: 9000000 INFO @ Thu, 16 Apr 2020 02:59:30: 21000000 INFO @ Thu, 16 Apr 2020 02:59:32: 16000000 INFO @ Thu, 16 Apr 2020 02:59:36: 10000000 INFO @ Thu, 16 Apr 2020 02:59:36: 22000000 INFO @ Thu, 16 Apr 2020 02:59:37: 17000000 INFO @ Thu, 16 Apr 2020 02:59:42: 23000000 INFO @ Thu, 16 Apr 2020 02:59:42: 11000000 INFO @ Thu, 16 Apr 2020 02:59:43: 18000000 INFO @ Thu, 16 Apr 2020 02:59:48: 24000000 INFO @ Thu, 16 Apr 2020 02:59:49: 19000000 INFO @ Thu, 16 Apr 2020 02:59:49: 12000000 INFO @ Thu, 16 Apr 2020 02:59:54: 20000000 INFO @ Thu, 16 Apr 2020 02:59:55: 25000000 INFO @ Thu, 16 Apr 2020 02:59:56: 13000000 INFO @ Thu, 16 Apr 2020 03:00:00: 21000000 INFO @ Thu, 16 Apr 2020 03:00:01: 26000000 INFO @ Thu, 16 Apr 2020 03:00:03: 14000000 INFO @ Thu, 16 Apr 2020 03:00:06: 22000000 INFO @ Thu, 16 Apr 2020 03:00:07: 27000000 INFO @ Thu, 16 Apr 2020 03:00:09: 15000000 INFO @ Thu, 16 Apr 2020 03:00:11: 23000000 INFO @ Thu, 16 Apr 2020 03:00:13: 28000000 INFO @ Thu, 16 Apr 2020 03:00:16: 16000000 INFO @ Thu, 16 Apr 2020 03:00:17: 24000000 INFO @ Thu, 16 Apr 2020 03:00:19: 29000000 INFO @ Thu, 16 Apr 2020 03:00:23: 17000000 INFO @ Thu, 16 Apr 2020 03:00:23: 25000000 INFO @ Thu, 16 Apr 2020 03:00:24: 30000000 INFO @ Thu, 16 Apr 2020 03:00:29: 26000000 INFO @ Thu, 16 Apr 2020 03:00:29: 18000000 INFO @ Thu, 16 Apr 2020 03:00:30: 31000000 INFO @ Thu, 16 Apr 2020 03:00:35: 27000000 INFO @ Thu, 16 Apr 2020 03:00:36: 32000000 INFO @ Thu, 16 Apr 2020 03:00:36: 19000000 INFO @ Thu, 16 Apr 2020 03:00:40: 28000000 INFO @ Thu, 16 Apr 2020 03:00:41: 33000000 INFO @ Thu, 16 Apr 2020 03:00:42: 20000000 INFO @ Thu, 16 Apr 2020 03:00:45: #1 tag size is determined as 50 bps INFO @ Thu, 16 Apr 2020 03:00:45: #1 tag size = 50 INFO @ Thu, 16 Apr 2020 03:00:45: #1 total tags in treatment: 33558839 INFO @ Thu, 16 Apr 2020 03:00:45: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 03:00:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 03:00:45: #1 tags after filtering in treatment: 33558839 INFO @ Thu, 16 Apr 2020 03:00:45: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 03:00:45: #1 finished! INFO @ Thu, 16 Apr 2020 03:00:45: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 03:00:45: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 03:00:46: 29000000 INFO @ Thu, 16 Apr 2020 03:00:47: #2 number of paired peaks: 98 WARNING @ Thu, 16 Apr 2020 03:00:47: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 16 Apr 2020 03:00:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 03:00:49: 21000000 INFO @ Thu, 16 Apr 2020 03:00:52: 30000000 INFO @ Thu, 16 Apr 2020 03:00:55: 22000000 INFO @ Thu, 16 Apr 2020 03:00:57: 31000000 INFO @ Thu, 16 Apr 2020 03:01:02: 23000000 INFO @ Thu, 16 Apr 2020 03:01:03: 32000000 INFO @ Thu, 16 Apr 2020 03:01:09: 33000000 INFO @ Thu, 16 Apr 2020 03:01:09: 24000000 INFO @ Thu, 16 Apr 2020 03:01:12: #1 tag size is determined as 50 bps INFO @ Thu, 16 Apr 2020 03:01:12: #1 tag size = 50 INFO @ Thu, 16 Apr 2020 03:01:12: #1 total tags in treatment: 33558839 INFO @ Thu, 16 Apr 2020 03:01:12: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 03:01:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 03:01:13: #1 tags after filtering in treatment: 33558839 INFO @ Thu, 16 Apr 2020 03:01:13: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 03:01:13: #1 finished! INFO @ Thu, 16 Apr 2020 03:01:13: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 03:01:13: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 03:01:15: #2 number of paired peaks: 98 WARNING @ Thu, 16 Apr 2020 03:01:15: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 16 Apr 2020 03:01:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 03:01:16: 25000000 INFO @ Thu, 16 Apr 2020 03:01:22: 26000000 INFO @ Thu, 16 Apr 2020 03:01:29: 27000000 INFO @ Thu, 16 Apr 2020 03:01:35: 28000000 INFO @ Thu, 16 Apr 2020 03:01:42: 29000000 INFO @ Thu, 16 Apr 2020 03:01:48: 30000000 INFO @ Thu, 16 Apr 2020 03:01:55: 31000000 INFO @ Thu, 16 Apr 2020 03:02:01: 32000000 INFO @ Thu, 16 Apr 2020 03:02:08: 33000000 INFO @ Thu, 16 Apr 2020 03:02:11: #1 tag size is determined as 50 bps INFO @ Thu, 16 Apr 2020 03:02:11: #1 tag size = 50 INFO @ Thu, 16 Apr 2020 03:02:11: #1 total tags in treatment: 33558839 INFO @ Thu, 16 Apr 2020 03:02:11: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 03:02:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 03:02:12: #1 tags after filtering in treatment: 33558839 INFO @ Thu, 16 Apr 2020 03:02:12: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 03:02:12: #1 finished! INFO @ Thu, 16 Apr 2020 03:02:12: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 03:02:12: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 03:02:14: #2 number of paired peaks: 98 WARNING @ Thu, 16 Apr 2020 03:02:14: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 16 Apr 2020 03:02:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5416215/SRX5416215.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。