Job ID = 6528352 SRX = SRX5379465 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:46:58 prefetch.2.10.7: 1) Downloading 'SRR8578659'... 2020-06-29T14:46:58 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:48:39 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:48:39 prefetch.2.10.7: 1) 'SRR8578659' was downloaded successfully 2020-06-29T14:48:39 prefetch.2.10.7: 'SRR8578659' has 0 unresolved dependencies Read 33363864 spots for SRR8578659/SRR8578659.sra Written 33363864 spots for SRR8578659/SRR8578659.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:38 33363864 reads; of these: 33363864 (100.00%) were unpaired; of these: 1259133 (3.77%) aligned 0 times 26971190 (80.84%) aligned exactly 1 time 5133541 (15.39%) aligned >1 times 96.23% overall alignment rate Time searching: 00:08:39 Overall time: 00:08:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4142237 / 32104731 = 0.1290 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:12:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:12:06: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:12:06: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:12:11: 1000000 INFO @ Tue, 30 Jun 2020 00:12:16: 2000000 INFO @ Tue, 30 Jun 2020 00:12:21: 3000000 INFO @ Tue, 30 Jun 2020 00:12:26: 4000000 INFO @ Tue, 30 Jun 2020 00:12:31: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:12:35: 6000000 INFO @ Tue, 30 Jun 2020 00:12:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:12:36: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:12:36: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:12:40: 7000000 INFO @ Tue, 30 Jun 2020 00:12:41: 1000000 INFO @ Tue, 30 Jun 2020 00:12:45: 8000000 INFO @ Tue, 30 Jun 2020 00:12:47: 2000000 INFO @ Tue, 30 Jun 2020 00:12:51: 9000000 INFO @ Tue, 30 Jun 2020 00:12:52: 3000000 INFO @ Tue, 30 Jun 2020 00:12:56: 10000000 INFO @ Tue, 30 Jun 2020 00:12:57: 4000000 INFO @ Tue, 30 Jun 2020 00:13:01: 11000000 INFO @ Tue, 30 Jun 2020 00:13:02: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:13:06: 12000000 INFO @ Tue, 30 Jun 2020 00:13:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:13:06: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:13:06: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:13:07: 6000000 INFO @ Tue, 30 Jun 2020 00:13:11: 13000000 INFO @ Tue, 30 Jun 2020 00:13:12: 1000000 INFO @ Tue, 30 Jun 2020 00:13:12: 7000000 INFO @ Tue, 30 Jun 2020 00:13:16: 14000000 INFO @ Tue, 30 Jun 2020 00:13:17: 2000000 INFO @ Tue, 30 Jun 2020 00:13:17: 8000000 INFO @ Tue, 30 Jun 2020 00:13:21: 15000000 INFO @ Tue, 30 Jun 2020 00:13:22: 3000000 INFO @ Tue, 30 Jun 2020 00:13:22: 9000000 INFO @ Tue, 30 Jun 2020 00:13:26: 16000000 INFO @ Tue, 30 Jun 2020 00:13:27: 4000000 INFO @ Tue, 30 Jun 2020 00:13:28: 10000000 INFO @ Tue, 30 Jun 2020 00:13:31: 17000000 INFO @ Tue, 30 Jun 2020 00:13:32: 5000000 INFO @ Tue, 30 Jun 2020 00:13:33: 11000000 INFO @ Tue, 30 Jun 2020 00:13:36: 18000000 INFO @ Tue, 30 Jun 2020 00:13:38: 6000000 INFO @ Tue, 30 Jun 2020 00:13:38: 12000000 INFO @ Tue, 30 Jun 2020 00:13:41: 19000000 INFO @ Tue, 30 Jun 2020 00:13:43: 7000000 INFO @ Tue, 30 Jun 2020 00:13:43: 13000000 INFO @ Tue, 30 Jun 2020 00:13:47: 20000000 INFO @ Tue, 30 Jun 2020 00:13:48: 8000000 INFO @ Tue, 30 Jun 2020 00:13:48: 14000000 INFO @ Tue, 30 Jun 2020 00:13:52: 21000000 INFO @ Tue, 30 Jun 2020 00:13:53: 9000000 INFO @ Tue, 30 Jun 2020 00:13:53: 15000000 INFO @ Tue, 30 Jun 2020 00:13:57: 22000000 INFO @ Tue, 30 Jun 2020 00:13:58: 10000000 INFO @ Tue, 30 Jun 2020 00:13:58: 16000000 INFO @ Tue, 30 Jun 2020 00:14:02: 23000000 INFO @ Tue, 30 Jun 2020 00:14:04: 17000000 INFO @ Tue, 30 Jun 2020 00:14:04: 11000000 INFO @ Tue, 30 Jun 2020 00:14:07: 24000000 INFO @ Tue, 30 Jun 2020 00:14:09: 18000000 INFO @ Tue, 30 Jun 2020 00:14:09: 12000000 INFO @ Tue, 30 Jun 2020 00:14:13: 25000000 INFO @ Tue, 30 Jun 2020 00:14:14: 19000000 INFO @ Tue, 30 Jun 2020 00:14:15: 13000000 INFO @ Tue, 30 Jun 2020 00:14:18: 26000000 INFO @ Tue, 30 Jun 2020 00:14:20: 20000000 INFO @ Tue, 30 Jun 2020 00:14:20: 14000000 INFO @ Tue, 30 Jun 2020 00:14:24: 27000000 INFO @ Tue, 30 Jun 2020 00:14:25: 21000000 INFO @ Tue, 30 Jun 2020 00:14:25: 15000000 INFO @ Tue, 30 Jun 2020 00:14:29: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:14:29: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:14:29: #1 total tags in treatment: 27962494 INFO @ Tue, 30 Jun 2020 00:14:29: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:14:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:14:29: #1 tags after filtering in treatment: 27962494 INFO @ Tue, 30 Jun 2020 00:14:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:14:29: #1 finished! INFO @ Tue, 30 Jun 2020 00:14:29: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:14:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:14:30: 22000000 INFO @ Tue, 30 Jun 2020 00:14:31: 16000000 INFO @ Tue, 30 Jun 2020 00:14:31: #2 number of paired peaks: 67 WARNING @ Tue, 30 Jun 2020 00:14:31: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:14:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:14:35: 23000000 INFO @ Tue, 30 Jun 2020 00:14:36: 17000000 INFO @ Tue, 30 Jun 2020 00:14:40: 24000000 INFO @ Tue, 30 Jun 2020 00:14:41: 18000000 INFO @ Tue, 30 Jun 2020 00:14:45: 25000000 INFO @ Tue, 30 Jun 2020 00:14:46: 19000000 INFO @ Tue, 30 Jun 2020 00:14:51: 26000000 INFO @ Tue, 30 Jun 2020 00:14:52: 20000000 INFO @ Tue, 30 Jun 2020 00:14:57: 27000000 INFO @ Tue, 30 Jun 2020 00:14:57: 21000000 INFO @ Tue, 30 Jun 2020 00:15:02: 22000000 INFO @ Tue, 30 Jun 2020 00:15:03: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:15:03: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:15:03: #1 total tags in treatment: 27962494 INFO @ Tue, 30 Jun 2020 00:15:03: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:15:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:15:03: #1 tags after filtering in treatment: 27962494 INFO @ Tue, 30 Jun 2020 00:15:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:15:03: #1 finished! INFO @ Tue, 30 Jun 2020 00:15:03: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:15:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:15:05: #2 number of paired peaks: 67 WARNING @ Tue, 30 Jun 2020 00:15:05: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:15:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:15:08: 23000000 INFO @ Tue, 30 Jun 2020 00:15:13: 24000000 INFO @ Tue, 30 Jun 2020 00:15:18: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:15:23: 26000000 INFO @ Tue, 30 Jun 2020 00:15:28: 27000000 INFO @ Tue, 30 Jun 2020 00:15:33: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:15:33: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:15:33: #1 total tags in treatment: 27962494 INFO @ Tue, 30 Jun 2020 00:15:33: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:15:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:15:33: #1 tags after filtering in treatment: 27962494 INFO @ Tue, 30 Jun 2020 00:15:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:15:33: #1 finished! INFO @ Tue, 30 Jun 2020 00:15:33: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:15:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:15:35: #2 number of paired peaks: 67 WARNING @ Tue, 30 Jun 2020 00:15:35: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:15:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5379465/SRX5379465.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。