Job ID = 6528323 SRX = SRX5343148 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:36:57 prefetch.2.10.7: 1) Downloading 'SRR8540848'... 2020-06-29T15:36:57 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:41:04 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:41:04 prefetch.2.10.7: 1) 'SRR8540848' was downloaded successfully 2020-06-29T15:41:04 prefetch.2.10.7: 'SRR8540848' has 0 unresolved dependencies Read 34725896 spots for SRR8540848/SRR8540848.sra Written 34725896 spots for SRR8540848/SRR8540848.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:12 34725896 reads; of these: 34725896 (100.00%) were unpaired; of these: 1276475 (3.68%) aligned 0 times 22990209 (66.20%) aligned exactly 1 time 10459212 (30.12%) aligned >1 times 96.32% overall alignment rate Time searching: 00:17:12 Overall time: 00:17:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4348494 / 33449421 = 0.1300 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:28:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:28:34: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:28:34: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:28:42: 1000000 INFO @ Tue, 30 Jun 2020 01:28:52: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:29:01: 3000000 INFO @ Tue, 30 Jun 2020 01:29:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:29:02: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:29:02: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:29:11: 1000000 INFO @ Tue, 30 Jun 2020 01:29:11: 4000000 INFO @ Tue, 30 Jun 2020 01:29:18: 2000000 INFO @ Tue, 30 Jun 2020 01:29:20: 5000000 INFO @ Tue, 30 Jun 2020 01:29:25: 3000000 INFO @ Tue, 30 Jun 2020 01:29:30: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:29:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:29:32: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:29:32: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:29:33: 4000000 INFO @ Tue, 30 Jun 2020 01:29:39: 7000000 INFO @ Tue, 30 Jun 2020 01:29:41: 5000000 INFO @ Tue, 30 Jun 2020 01:29:43: 1000000 INFO @ Tue, 30 Jun 2020 01:29:49: 8000000 INFO @ Tue, 30 Jun 2020 01:29:50: 6000000 INFO @ Tue, 30 Jun 2020 01:29:53: 2000000 INFO @ Tue, 30 Jun 2020 01:29:58: 9000000 INFO @ Tue, 30 Jun 2020 01:29:59: 7000000 INFO @ Tue, 30 Jun 2020 01:30:02: 3000000 INFO @ Tue, 30 Jun 2020 01:30:08: 10000000 INFO @ Tue, 30 Jun 2020 01:30:08: 8000000 INFO @ Tue, 30 Jun 2020 01:30:12: 4000000 INFO @ Tue, 30 Jun 2020 01:30:17: 9000000 INFO @ Tue, 30 Jun 2020 01:30:18: 11000000 INFO @ Tue, 30 Jun 2020 01:30:21: 5000000 INFO @ Tue, 30 Jun 2020 01:30:25: 10000000 INFO @ Tue, 30 Jun 2020 01:30:27: 12000000 INFO @ Tue, 30 Jun 2020 01:30:30: 6000000 INFO @ Tue, 30 Jun 2020 01:30:34: 11000000 INFO @ Tue, 30 Jun 2020 01:30:37: 13000000 INFO @ Tue, 30 Jun 2020 01:30:39: 7000000 INFO @ Tue, 30 Jun 2020 01:30:42: 12000000 INFO @ Tue, 30 Jun 2020 01:30:48: 14000000 INFO @ Tue, 30 Jun 2020 01:30:49: 8000000 INFO @ Tue, 30 Jun 2020 01:30:51: 13000000 INFO @ Tue, 30 Jun 2020 01:30:57: 15000000 INFO @ Tue, 30 Jun 2020 01:30:58: 9000000 INFO @ Tue, 30 Jun 2020 01:31:00: 14000000 INFO @ Tue, 30 Jun 2020 01:31:07: 10000000 INFO @ Tue, 30 Jun 2020 01:31:09: 16000000 INFO @ Tue, 30 Jun 2020 01:31:09: 15000000 INFO @ Tue, 30 Jun 2020 01:31:16: 11000000 INFO @ Tue, 30 Jun 2020 01:31:18: 16000000 INFO @ Tue, 30 Jun 2020 01:31:18: 17000000 INFO @ Tue, 30 Jun 2020 01:31:25: 12000000 INFO @ Tue, 30 Jun 2020 01:31:26: 17000000 INFO @ Tue, 30 Jun 2020 01:31:29: 18000000 INFO @ Tue, 30 Jun 2020 01:31:35: 13000000 INFO @ Tue, 30 Jun 2020 01:31:35: 18000000 INFO @ Tue, 30 Jun 2020 01:31:39: 19000000 INFO @ Tue, 30 Jun 2020 01:31:44: 14000000 INFO @ Tue, 30 Jun 2020 01:31:44: 19000000 INFO @ Tue, 30 Jun 2020 01:31:48: 20000000 INFO @ Tue, 30 Jun 2020 01:31:53: 20000000 INFO @ Tue, 30 Jun 2020 01:31:53: 15000000 INFO @ Tue, 30 Jun 2020 01:31:58: 21000000 INFO @ Tue, 30 Jun 2020 01:32:02: 21000000 INFO @ Tue, 30 Jun 2020 01:32:03: 16000000 INFO @ Tue, 30 Jun 2020 01:32:07: 22000000 INFO @ Tue, 30 Jun 2020 01:32:10: 22000000 INFO @ Tue, 30 Jun 2020 01:32:12: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:32:17: 23000000 INFO @ Tue, 30 Jun 2020 01:32:19: 23000000 INFO @ Tue, 30 Jun 2020 01:32:21: 18000000 INFO @ Tue, 30 Jun 2020 01:32:26: 24000000 INFO @ Tue, 30 Jun 2020 01:32:28: 24000000 INFO @ Tue, 30 Jun 2020 01:32:30: 19000000 INFO @ Tue, 30 Jun 2020 01:32:35: 25000000 INFO @ Tue, 30 Jun 2020 01:32:37: 25000000 INFO @ Tue, 30 Jun 2020 01:32:39: 20000000 INFO @ Tue, 30 Jun 2020 01:32:44: 26000000 INFO @ Tue, 30 Jun 2020 01:32:46: 26000000 INFO @ Tue, 30 Jun 2020 01:32:48: 21000000 INFO @ Tue, 30 Jun 2020 01:32:54: 27000000 INFO @ Tue, 30 Jun 2020 01:32:55: 27000000 INFO @ Tue, 30 Jun 2020 01:32:57: 22000000 INFO @ Tue, 30 Jun 2020 01:33:03: 28000000 INFO @ Tue, 30 Jun 2020 01:33:04: 28000000 INFO @ Tue, 30 Jun 2020 01:33:07: 23000000 INFO @ Tue, 30 Jun 2020 01:33:12: 29000000 INFO @ Tue, 30 Jun 2020 01:33:13: 29000000 INFO @ Tue, 30 Jun 2020 01:33:13: #1 tag size is determined as 65 bps INFO @ Tue, 30 Jun 2020 01:33:13: #1 tag size = 65 INFO @ Tue, 30 Jun 2020 01:33:13: #1 total tags in treatment: 29100927 INFO @ Tue, 30 Jun 2020 01:33:13: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:33:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:33:14: #1 tag size is determined as 65 bps INFO @ Tue, 30 Jun 2020 01:33:14: #1 tag size = 65 INFO @ Tue, 30 Jun 2020 01:33:14: #1 total tags in treatment: 29100927 INFO @ Tue, 30 Jun 2020 01:33:14: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:33:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:33:14: #1 tags after filtering in treatment: 29100927 INFO @ Tue, 30 Jun 2020 01:33:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:33:14: #1 finished! INFO @ Tue, 30 Jun 2020 01:33:14: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:33:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:33:14: #1 tags after filtering in treatment: 29100927 INFO @ Tue, 30 Jun 2020 01:33:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:33:14: #1 finished! INFO @ Tue, 30 Jun 2020 01:33:14: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:33:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:33:15: 24000000 INFO @ Tue, 30 Jun 2020 01:33:15: #2 number of paired peaks: 49 WARNING @ Tue, 30 Jun 2020 01:33:15: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:33:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:33:16: #2 number of paired peaks: 49 WARNING @ Tue, 30 Jun 2020 01:33:16: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:33:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:33:23: 25000000 INFO @ Tue, 30 Jun 2020 01:33:31: 26000000 INFO @ Tue, 30 Jun 2020 01:33:39: 27000000 BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 01:33:48: 28000000 INFO @ Tue, 30 Jun 2020 01:33:56: 29000000 INFO @ Tue, 30 Jun 2020 01:33:57: #1 tag size is determined as 65 bps INFO @ Tue, 30 Jun 2020 01:33:57: #1 tag size = 65 INFO @ Tue, 30 Jun 2020 01:33:57: #1 total tags in treatment: 29100927 INFO @ Tue, 30 Jun 2020 01:33:57: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:33:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:33:57: #1 tags after filtering in treatment: 29100927 INFO @ Tue, 30 Jun 2020 01:33:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:33:57: #1 finished! INFO @ Tue, 30 Jun 2020 01:33:57: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:33:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:33:59: #2 number of paired peaks: 49 WARNING @ Tue, 30 Jun 2020 01:33:59: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:33:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343148/SRX5343148.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling