Job ID = 6528316 SRX = SRX5343136 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:21:49 prefetch.2.10.7: 1) Downloading 'SRR8540836'... 2020-06-29T15:21:49 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:25:12 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:25:12 prefetch.2.10.7: 1) 'SRR8540836' was downloaded successfully 2020-06-29T15:25:12 prefetch.2.10.7: 'SRR8540836' has 0 unresolved dependencies Read 23202652 spots for SRR8540836/SRR8540836.sra Written 23202652 spots for SRR8540836/SRR8540836.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:43 23202652 reads; of these: 23202652 (100.00%) were unpaired; of these: 1092053 (4.71%) aligned 0 times 18891502 (81.42%) aligned exactly 1 time 3219097 (13.87%) aligned >1 times 95.29% overall alignment rate Time searching: 00:07:43 Overall time: 00:07:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 1700599 / 22110599 = 0.0769 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:53:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:53:35: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:53:35: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:53:44: 1000000 INFO @ Tue, 30 Jun 2020 00:53:53: 2000000 INFO @ Tue, 30 Jun 2020 00:54:02: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:54:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:54:05: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:54:05: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:54:11: 4000000 INFO @ Tue, 30 Jun 2020 00:54:13: 1000000 INFO @ Tue, 30 Jun 2020 00:54:20: 5000000 INFO @ Tue, 30 Jun 2020 00:54:21: 2000000 INFO @ Tue, 30 Jun 2020 00:54:29: 6000000 INFO @ Tue, 30 Jun 2020 00:54:29: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:54:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:54:35: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:54:35: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:54:38: 4000000 INFO @ Tue, 30 Jun 2020 00:54:38: 7000000 INFO @ Tue, 30 Jun 2020 00:54:44: 1000000 INFO @ Tue, 30 Jun 2020 00:54:46: 5000000 INFO @ Tue, 30 Jun 2020 00:54:47: 8000000 INFO @ Tue, 30 Jun 2020 00:54:54: 2000000 INFO @ Tue, 30 Jun 2020 00:54:55: 6000000 INFO @ Tue, 30 Jun 2020 00:54:57: 9000000 INFO @ Tue, 30 Jun 2020 00:55:02: 3000000 INFO @ Tue, 30 Jun 2020 00:55:04: 7000000 INFO @ Tue, 30 Jun 2020 00:55:06: 10000000 INFO @ Tue, 30 Jun 2020 00:55:11: 4000000 INFO @ Tue, 30 Jun 2020 00:55:13: 8000000 INFO @ Tue, 30 Jun 2020 00:55:15: 11000000 INFO @ Tue, 30 Jun 2020 00:55:20: 5000000 INFO @ Tue, 30 Jun 2020 00:55:21: 9000000 INFO @ Tue, 30 Jun 2020 00:55:24: 12000000 INFO @ Tue, 30 Jun 2020 00:55:29: 6000000 INFO @ Tue, 30 Jun 2020 00:55:30: 10000000 INFO @ Tue, 30 Jun 2020 00:55:34: 13000000 INFO @ Tue, 30 Jun 2020 00:55:38: 7000000 INFO @ Tue, 30 Jun 2020 00:55:39: 11000000 INFO @ Tue, 30 Jun 2020 00:55:43: 14000000 INFO @ Tue, 30 Jun 2020 00:55:47: 8000000 INFO @ Tue, 30 Jun 2020 00:55:48: 12000000 INFO @ Tue, 30 Jun 2020 00:55:52: 15000000 INFO @ Tue, 30 Jun 2020 00:55:56: 9000000 INFO @ Tue, 30 Jun 2020 00:55:56: 13000000 INFO @ Tue, 30 Jun 2020 00:56:01: 16000000 INFO @ Tue, 30 Jun 2020 00:56:05: 10000000 INFO @ Tue, 30 Jun 2020 00:56:05: 14000000 INFO @ Tue, 30 Jun 2020 00:56:11: 17000000 INFO @ Tue, 30 Jun 2020 00:56:14: 11000000 INFO @ Tue, 30 Jun 2020 00:56:14: 15000000 INFO @ Tue, 30 Jun 2020 00:56:20: 18000000 INFO @ Tue, 30 Jun 2020 00:56:22: 12000000 INFO @ Tue, 30 Jun 2020 00:56:23: 16000000 INFO @ Tue, 30 Jun 2020 00:56:30: 19000000 INFO @ Tue, 30 Jun 2020 00:56:31: 13000000 INFO @ Tue, 30 Jun 2020 00:56:32: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:56:39: 20000000 INFO @ Tue, 30 Jun 2020 00:56:40: 14000000 INFO @ Tue, 30 Jun 2020 00:56:41: 18000000 INFO @ Tue, 30 Jun 2020 00:56:43: #1 tag size is determined as 65 bps INFO @ Tue, 30 Jun 2020 00:56:43: #1 tag size = 65 INFO @ Tue, 30 Jun 2020 00:56:43: #1 total tags in treatment: 20410000 INFO @ Tue, 30 Jun 2020 00:56:43: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:56:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:56:43: #1 tags after filtering in treatment: 20410000 INFO @ Tue, 30 Jun 2020 00:56:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:56:43: #1 finished! INFO @ Tue, 30 Jun 2020 00:56:43: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:56:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:56:44: #2 number of paired peaks: 50 WARNING @ Tue, 30 Jun 2020 00:56:44: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:56:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:56:49: 15000000 INFO @ Tue, 30 Jun 2020 00:56:49: 19000000 INFO @ Tue, 30 Jun 2020 00:56:57: 16000000 INFO @ Tue, 30 Jun 2020 00:56:58: 20000000 INFO @ Tue, 30 Jun 2020 00:57:01: #1 tag size is determined as 65 bps INFO @ Tue, 30 Jun 2020 00:57:01: #1 tag size = 65 INFO @ Tue, 30 Jun 2020 00:57:01: #1 total tags in treatment: 20410000 INFO @ Tue, 30 Jun 2020 00:57:01: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:57:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:57:01: #1 tags after filtering in treatment: 20410000 INFO @ Tue, 30 Jun 2020 00:57:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:57:01: #1 finished! INFO @ Tue, 30 Jun 2020 00:57:01: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:57:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:57:03: #2 number of paired peaks: 50 WARNING @ Tue, 30 Jun 2020 00:57:03: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:57:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:57:05: 17000000 INFO @ Tue, 30 Jun 2020 00:57:12: 18000000 INFO @ Tue, 30 Jun 2020 00:57:20: 19000000 INFO @ Tue, 30 Jun 2020 00:57:28: 20000000 INFO @ Tue, 30 Jun 2020 00:57:31: #1 tag size is determined as 65 bps INFO @ Tue, 30 Jun 2020 00:57:31: #1 tag size = 65 INFO @ Tue, 30 Jun 2020 00:57:31: #1 total tags in treatment: 20410000 INFO @ Tue, 30 Jun 2020 00:57:31: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:57:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:57:31: #1 tags after filtering in treatment: 20410000 INFO @ Tue, 30 Jun 2020 00:57:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:57:31: #1 finished! INFO @ Tue, 30 Jun 2020 00:57:31: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:57:31: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 00:57:32: #2 number of paired peaks: 50 WARNING @ Tue, 30 Jun 2020 00:57:32: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:57:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343136/SRX5343136.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling