Job ID = 6528307 SRX = SRX5343099 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:52:59 prefetch.2.10.7: 1) Downloading 'SRR8540876'... 2020-06-29T14:52:59 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:55:01 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:55:01 prefetch.2.10.7: 1) 'SRR8540876' was downloaded successfully 2020-06-29T14:55:01 prefetch.2.10.7: 'SRR8540876' has 0 unresolved dependencies Read 23202655 spots for SRR8540876/SRR8540876.sra Written 23202655 spots for SRR8540876/SRR8540876.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:47 23202655 reads; of these: 23202655 (100.00%) were unpaired; of these: 1091997 (4.71%) aligned 0 times 18891469 (81.42%) aligned exactly 1 time 3219189 (13.87%) aligned >1 times 95.29% overall alignment rate Time searching: 00:07:47 Overall time: 00:07:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 1699932 / 22110658 = 0.0769 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:18:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:18:26: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:18:26: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:18:34: 1000000 INFO @ Tue, 30 Jun 2020 00:18:41: 2000000 INFO @ Tue, 30 Jun 2020 00:18:48: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:18:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:18:56: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:18:56: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:18:56: 4000000 INFO @ Tue, 30 Jun 2020 00:19:04: 1000000 INFO @ Tue, 30 Jun 2020 00:19:04: 5000000 INFO @ Tue, 30 Jun 2020 00:19:11: 2000000 INFO @ Tue, 30 Jun 2020 00:19:13: 6000000 INFO @ Tue, 30 Jun 2020 00:19:19: 3000000 INFO @ Tue, 30 Jun 2020 00:19:22: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:19:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:19:26: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:19:26: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:19:27: 4000000 INFO @ Tue, 30 Jun 2020 00:19:30: 8000000 INFO @ Tue, 30 Jun 2020 00:19:34: 5000000 INFO @ Tue, 30 Jun 2020 00:19:35: 1000000 INFO @ Tue, 30 Jun 2020 00:19:39: 9000000 INFO @ Tue, 30 Jun 2020 00:19:42: 6000000 INFO @ Tue, 30 Jun 2020 00:19:43: 2000000 INFO @ Tue, 30 Jun 2020 00:19:47: 10000000 INFO @ Tue, 30 Jun 2020 00:19:50: 7000000 INFO @ Tue, 30 Jun 2020 00:19:52: 3000000 INFO @ Tue, 30 Jun 2020 00:19:56: 11000000 INFO @ Tue, 30 Jun 2020 00:19:57: 8000000 INFO @ Tue, 30 Jun 2020 00:20:00: 4000000 INFO @ Tue, 30 Jun 2020 00:20:04: 12000000 INFO @ Tue, 30 Jun 2020 00:20:05: 9000000 INFO @ Tue, 30 Jun 2020 00:20:09: 5000000 INFO @ Tue, 30 Jun 2020 00:20:13: 13000000 INFO @ Tue, 30 Jun 2020 00:20:13: 10000000 INFO @ Tue, 30 Jun 2020 00:20:17: 6000000 INFO @ Tue, 30 Jun 2020 00:20:21: 11000000 INFO @ Tue, 30 Jun 2020 00:20:21: 14000000 INFO @ Tue, 30 Jun 2020 00:20:26: 7000000 INFO @ Tue, 30 Jun 2020 00:20:29: 12000000 INFO @ Tue, 30 Jun 2020 00:20:30: 15000000 INFO @ Tue, 30 Jun 2020 00:20:35: 8000000 INFO @ Tue, 30 Jun 2020 00:20:36: 13000000 INFO @ Tue, 30 Jun 2020 00:20:38: 16000000 INFO @ Tue, 30 Jun 2020 00:20:43: 9000000 INFO @ Tue, 30 Jun 2020 00:20:44: 14000000 INFO @ Tue, 30 Jun 2020 00:20:47: 17000000 INFO @ Tue, 30 Jun 2020 00:20:52: 15000000 INFO @ Tue, 30 Jun 2020 00:20:52: 10000000 INFO @ Tue, 30 Jun 2020 00:20:57: 18000000 INFO @ Tue, 30 Jun 2020 00:20:59: 16000000 INFO @ Tue, 30 Jun 2020 00:21:01: 11000000 INFO @ Tue, 30 Jun 2020 00:21:06: 19000000 INFO @ Tue, 30 Jun 2020 00:21:07: 17000000 INFO @ Tue, 30 Jun 2020 00:21:10: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:21:15: 20000000 INFO @ Tue, 30 Jun 2020 00:21:15: 18000000 INFO @ Tue, 30 Jun 2020 00:21:18: #1 tag size is determined as 65 bps INFO @ Tue, 30 Jun 2020 00:21:18: #1 tag size = 65 INFO @ Tue, 30 Jun 2020 00:21:18: #1 total tags in treatment: 20410726 INFO @ Tue, 30 Jun 2020 00:21:18: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:21:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:21:19: #1 tags after filtering in treatment: 20410726 INFO @ Tue, 30 Jun 2020 00:21:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:21:19: #1 finished! INFO @ Tue, 30 Jun 2020 00:21:19: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:21:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:21:19: 13000000 INFO @ Tue, 30 Jun 2020 00:21:20: #2 number of paired peaks: 52 WARNING @ Tue, 30 Jun 2020 00:21:20: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:21:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:21:23: 19000000 INFO @ Tue, 30 Jun 2020 00:21:27: 14000000 INFO @ Tue, 30 Jun 2020 00:21:31: 20000000 INFO @ Tue, 30 Jun 2020 00:21:34: #1 tag size is determined as 65 bps INFO @ Tue, 30 Jun 2020 00:21:34: #1 tag size = 65 INFO @ Tue, 30 Jun 2020 00:21:34: #1 total tags in treatment: 20410726 INFO @ Tue, 30 Jun 2020 00:21:34: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:21:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:21:34: #1 tags after filtering in treatment: 20410726 INFO @ Tue, 30 Jun 2020 00:21:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:21:34: #1 finished! INFO @ Tue, 30 Jun 2020 00:21:34: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:21:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:21:35: 15000000 INFO @ Tue, 30 Jun 2020 00:21:35: #2 number of paired peaks: 52 WARNING @ Tue, 30 Jun 2020 00:21:35: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:21:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:21:43: 16000000 INFO @ Tue, 30 Jun 2020 00:21:51: 17000000 BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 00:21:59: 18000000 INFO @ Tue, 30 Jun 2020 00:22:06: 19000000 INFO @ Tue, 30 Jun 2020 00:22:14: 20000000 INFO @ Tue, 30 Jun 2020 00:22:17: #1 tag size is determined as 65 bps INFO @ Tue, 30 Jun 2020 00:22:17: #1 tag size = 65 INFO @ Tue, 30 Jun 2020 00:22:17: #1 total tags in treatment: 20410726 INFO @ Tue, 30 Jun 2020 00:22:17: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:22:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:22:17: #1 tags after filtering in treatment: 20410726 INFO @ Tue, 30 Jun 2020 00:22:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:22:17: #1 finished! INFO @ Tue, 30 Jun 2020 00:22:17: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:22:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:22:18: #2 number of paired peaks: 52 WARNING @ Tue, 30 Jun 2020 00:22:18: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:22:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343099/SRX5343099.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling