Job ID = 12265316 SRX = SRX5287836 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 73805409 spots for SRR8483067/SRR8483067.sra Written 73805409 spots for SRR8483067/SRR8483067.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265539 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:36 73805409 reads; of these: 73805409 (100.00%) were unpaired; of these: 2902655 (3.93%) aligned 0 times 57462647 (77.86%) aligned exactly 1 time 13440107 (18.21%) aligned >1 times 96.07% overall alignment rate Time searching: 00:20:36 Overall time: 00:20:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 37199767 / 70902754 = 0.5247 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:17:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:17:15: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:17:15: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:17:22: 1000000 INFO @ Sat, 03 Apr 2021 07:17:28: 2000000 INFO @ Sat, 03 Apr 2021 07:17:35: 3000000 INFO @ Sat, 03 Apr 2021 07:17:41: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:17:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:17:45: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:17:45: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:17:48: 5000000 INFO @ Sat, 03 Apr 2021 07:17:52: 1000000 INFO @ Sat, 03 Apr 2021 07:17:55: 6000000 INFO @ Sat, 03 Apr 2021 07:18:00: 2000000 INFO @ Sat, 03 Apr 2021 07:18:02: 7000000 INFO @ Sat, 03 Apr 2021 07:18:08: 3000000 INFO @ Sat, 03 Apr 2021 07:18:10: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:18:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:18:15: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:18:15: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:18:16: 4000000 INFO @ Sat, 03 Apr 2021 07:18:18: 9000000 INFO @ Sat, 03 Apr 2021 07:18:23: 1000000 INFO @ Sat, 03 Apr 2021 07:18:24: 5000000 INFO @ Sat, 03 Apr 2021 07:18:26: 10000000 INFO @ Sat, 03 Apr 2021 07:18:31: 2000000 INFO @ Sat, 03 Apr 2021 07:18:31: 6000000 INFO @ Sat, 03 Apr 2021 07:18:35: 11000000 INFO @ Sat, 03 Apr 2021 07:18:38: 3000000 INFO @ Sat, 03 Apr 2021 07:18:39: 7000000 INFO @ Sat, 03 Apr 2021 07:18:43: 12000000 INFO @ Sat, 03 Apr 2021 07:18:46: 4000000 INFO @ Sat, 03 Apr 2021 07:18:47: 8000000 INFO @ Sat, 03 Apr 2021 07:18:51: 13000000 INFO @ Sat, 03 Apr 2021 07:18:53: 5000000 INFO @ Sat, 03 Apr 2021 07:18:55: 9000000 INFO @ Sat, 03 Apr 2021 07:18:59: 14000000 INFO @ Sat, 03 Apr 2021 07:19:00: 6000000 INFO @ Sat, 03 Apr 2021 07:19:03: 10000000 INFO @ Sat, 03 Apr 2021 07:19:06: 15000000 INFO @ Sat, 03 Apr 2021 07:19:07: 7000000 INFO @ Sat, 03 Apr 2021 07:19:11: 11000000 INFO @ Sat, 03 Apr 2021 07:19:14: 16000000 INFO @ Sat, 03 Apr 2021 07:19:14: 8000000 INFO @ Sat, 03 Apr 2021 07:19:18: 12000000 INFO @ Sat, 03 Apr 2021 07:19:21: 17000000 INFO @ Sat, 03 Apr 2021 07:19:22: 9000000 INFO @ Sat, 03 Apr 2021 07:19:25: 13000000 INFO @ Sat, 03 Apr 2021 07:19:28: 18000000 INFO @ Sat, 03 Apr 2021 07:19:29: 10000000 INFO @ Sat, 03 Apr 2021 07:19:32: 14000000 INFO @ Sat, 03 Apr 2021 07:19:35: 19000000 INFO @ Sat, 03 Apr 2021 07:19:36: 11000000 INFO @ Sat, 03 Apr 2021 07:19:40: 15000000 INFO @ Sat, 03 Apr 2021 07:19:43: 20000000 INFO @ Sat, 03 Apr 2021 07:19:44: 12000000 INFO @ Sat, 03 Apr 2021 07:19:49: 16000000 INFO @ Sat, 03 Apr 2021 07:19:51: 21000000 INFO @ Sat, 03 Apr 2021 07:19:52: 13000000 INFO @ Sat, 03 Apr 2021 07:19:58: 17000000 INFO @ Sat, 03 Apr 2021 07:20:00: 22000000 INFO @ Sat, 03 Apr 2021 07:20:00: 14000000 INFO @ Sat, 03 Apr 2021 07:20:07: 18000000 INFO @ Sat, 03 Apr 2021 07:20:07: 15000000 INFO @ Sat, 03 Apr 2021 07:20:08: 23000000 INFO @ Sat, 03 Apr 2021 07:20:15: 16000000 INFO @ Sat, 03 Apr 2021 07:20:15: 19000000 INFO @ Sat, 03 Apr 2021 07:20:17: 24000000 INFO @ Sat, 03 Apr 2021 07:20:23: 17000000 INFO @ Sat, 03 Apr 2021 07:20:24: 20000000 INFO @ Sat, 03 Apr 2021 07:20:26: 25000000 INFO @ Sat, 03 Apr 2021 07:20:31: 18000000 INFO @ Sat, 03 Apr 2021 07:20:33: 21000000 INFO @ Sat, 03 Apr 2021 07:20:35: 26000000 INFO @ Sat, 03 Apr 2021 07:20:39: 19000000 INFO @ Sat, 03 Apr 2021 07:20:42: 22000000 INFO @ Sat, 03 Apr 2021 07:20:44: 27000000 INFO @ Sat, 03 Apr 2021 07:20:47: 20000000 INFO @ Sat, 03 Apr 2021 07:20:51: 23000000 INFO @ Sat, 03 Apr 2021 07:20:53: 28000000 INFO @ Sat, 03 Apr 2021 07:20:55: 21000000 INFO @ Sat, 03 Apr 2021 07:20:59: 24000000 INFO @ Sat, 03 Apr 2021 07:21:02: 29000000 INFO @ Sat, 03 Apr 2021 07:21:02: 22000000 INFO @ Sat, 03 Apr 2021 07:21:08: 25000000 INFO @ Sat, 03 Apr 2021 07:21:09: 23000000 INFO @ Sat, 03 Apr 2021 07:21:11: 30000000 INFO @ Sat, 03 Apr 2021 07:21:17: 26000000 INFO @ Sat, 03 Apr 2021 07:21:17: 24000000 INFO @ Sat, 03 Apr 2021 07:21:20: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:21:26: 27000000 INFO @ Sat, 03 Apr 2021 07:21:26: 25000000 INFO @ Sat, 03 Apr 2021 07:21:29: 32000000 INFO @ Sat, 03 Apr 2021 07:21:33: 26000000 INFO @ Sat, 03 Apr 2021 07:21:35: 28000000 INFO @ Sat, 03 Apr 2021 07:21:38: 33000000 INFO @ Sat, 03 Apr 2021 07:21:41: 27000000 INFO @ Sat, 03 Apr 2021 07:21:44: 29000000 INFO @ Sat, 03 Apr 2021 07:21:44: #1 tag size is determined as 53 bps INFO @ Sat, 03 Apr 2021 07:21:44: #1 tag size = 53 INFO @ Sat, 03 Apr 2021 07:21:44: #1 total tags in treatment: 33702987 INFO @ Sat, 03 Apr 2021 07:21:44: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:21:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:21:45: #1 tags after filtering in treatment: 33702987 INFO @ Sat, 03 Apr 2021 07:21:45: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 07:21:45: #1 finished! INFO @ Sat, 03 Apr 2021 07:21:45: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:21:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:21:47: #2 number of paired peaks: 47 WARNING @ Sat, 03 Apr 2021 07:21:47: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:21:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:21:48: 28000000 INFO @ Sat, 03 Apr 2021 07:21:53: 30000000 INFO @ Sat, 03 Apr 2021 07:21:55: 29000000 INFO @ Sat, 03 Apr 2021 07:22:01: 31000000 INFO @ Sat, 03 Apr 2021 07:22:02: 30000000 INFO @ Sat, 03 Apr 2021 07:22:09: 31000000 INFO @ Sat, 03 Apr 2021 07:22:10: 32000000 INFO @ Sat, 03 Apr 2021 07:22:16: 32000000 INFO @ Sat, 03 Apr 2021 07:22:19: 33000000 INFO @ Sat, 03 Apr 2021 07:22:23: 33000000 INFO @ Sat, 03 Apr 2021 07:22:26: #1 tag size is determined as 53 bps INFO @ Sat, 03 Apr 2021 07:22:26: #1 tag size = 53 INFO @ Sat, 03 Apr 2021 07:22:26: #1 total tags in treatment: 33702987 INFO @ Sat, 03 Apr 2021 07:22:26: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:22:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:22:26: #1 tags after filtering in treatment: 33702987 INFO @ Sat, 03 Apr 2021 07:22:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 07:22:26: #1 finished! INFO @ Sat, 03 Apr 2021 07:22:26: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:22:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:22:28: #2 number of paired peaks: 47 WARNING @ Sat, 03 Apr 2021 07:22:28: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:22:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:22:29: #1 tag size is determined as 53 bps INFO @ Sat, 03 Apr 2021 07:22:29: #1 tag size = 53 INFO @ Sat, 03 Apr 2021 07:22:29: #1 total tags in treatment: 33702987 INFO @ Sat, 03 Apr 2021 07:22:29: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:22:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:22:30: #1 tags after filtering in treatment: 33702987 INFO @ Sat, 03 Apr 2021 07:22:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 07:22:30: #1 finished! INFO @ Sat, 03 Apr 2021 07:22:30: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:22:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:22:32: #2 number of paired peaks: 47 WARNING @ Sat, 03 Apr 2021 07:22:32: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:22:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287836/SRX5287836.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。