Job ID = 6528299 SRX = SRX5287832 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:00:55 prefetch.2.10.7: 1) Downloading 'SRR8483063'... 2020-06-29T15:00:55 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:04:43 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:04:43 prefetch.2.10.7: 1) 'SRR8483063' was downloaded successfully 2020-06-29T15:04:43 prefetch.2.10.7: 'SRR8483063' has 0 unresolved dependencies Read 29481911 spots for SRR8483063/SRR8483063.sra Written 29481911 spots for SRR8483063/SRR8483063.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:23 29481911 reads; of these: 29481911 (100.00%) were unpaired; of these: 951494 (3.23%) aligned 0 times 20490658 (69.50%) aligned exactly 1 time 8039759 (27.27%) aligned >1 times 96.77% overall alignment rate Time searching: 00:14:23 Overall time: 00:14:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3320505 / 28530417 = 0.1164 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:40:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:40:59: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:40:59: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:41:05: 1000000 INFO @ Tue, 30 Jun 2020 00:41:10: 2000000 INFO @ Tue, 30 Jun 2020 00:41:16: 3000000 INFO @ Tue, 30 Jun 2020 00:41:22: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:41:28: 5000000 INFO @ Tue, 30 Jun 2020 00:41:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:41:29: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:41:29: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:41:34: 6000000 INFO @ Tue, 30 Jun 2020 00:41:35: 1000000 INFO @ Tue, 30 Jun 2020 00:41:40: 7000000 INFO @ Tue, 30 Jun 2020 00:41:41: 2000000 INFO @ Tue, 30 Jun 2020 00:41:46: 8000000 INFO @ Tue, 30 Jun 2020 00:41:47: 3000000 INFO @ Tue, 30 Jun 2020 00:41:52: 9000000 INFO @ Tue, 30 Jun 2020 00:41:53: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:41:59: 10000000 INFO @ Tue, 30 Jun 2020 00:41:59: 5000000 INFO @ Tue, 30 Jun 2020 00:42:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:42:01: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:42:01: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:42:06: 6000000 INFO @ Tue, 30 Jun 2020 00:42:06: 11000000 INFO @ Tue, 30 Jun 2020 00:42:09: 1000000 INFO @ Tue, 30 Jun 2020 00:42:12: 7000000 INFO @ Tue, 30 Jun 2020 00:42:12: 12000000 INFO @ Tue, 30 Jun 2020 00:42:16: 2000000 INFO @ Tue, 30 Jun 2020 00:42:19: 8000000 INFO @ Tue, 30 Jun 2020 00:42:19: 13000000 INFO @ Tue, 30 Jun 2020 00:42:24: 3000000 INFO @ Tue, 30 Jun 2020 00:42:25: 9000000 INFO @ Tue, 30 Jun 2020 00:42:26: 14000000 INFO @ Tue, 30 Jun 2020 00:42:32: 4000000 INFO @ Tue, 30 Jun 2020 00:42:32: 10000000 INFO @ Tue, 30 Jun 2020 00:42:32: 15000000 INFO @ Tue, 30 Jun 2020 00:42:39: 11000000 INFO @ Tue, 30 Jun 2020 00:42:39: 5000000 INFO @ Tue, 30 Jun 2020 00:42:39: 16000000 INFO @ Tue, 30 Jun 2020 00:42:45: 12000000 INFO @ Tue, 30 Jun 2020 00:42:46: 17000000 INFO @ Tue, 30 Jun 2020 00:42:47: 6000000 INFO @ Tue, 30 Jun 2020 00:42:52: 13000000 INFO @ Tue, 30 Jun 2020 00:42:53: 18000000 INFO @ Tue, 30 Jun 2020 00:42:54: 7000000 INFO @ Tue, 30 Jun 2020 00:42:58: 14000000 INFO @ Tue, 30 Jun 2020 00:43:00: 19000000 INFO @ Tue, 30 Jun 2020 00:43:02: 8000000 INFO @ Tue, 30 Jun 2020 00:43:05: 15000000 INFO @ Tue, 30 Jun 2020 00:43:07: 20000000 INFO @ Tue, 30 Jun 2020 00:43:09: 9000000 INFO @ Tue, 30 Jun 2020 00:43:12: 16000000 INFO @ Tue, 30 Jun 2020 00:43:14: 21000000 INFO @ Tue, 30 Jun 2020 00:43:17: 10000000 INFO @ Tue, 30 Jun 2020 00:43:18: 17000000 INFO @ Tue, 30 Jun 2020 00:43:21: 22000000 INFO @ Tue, 30 Jun 2020 00:43:24: 11000000 INFO @ Tue, 30 Jun 2020 00:43:25: 18000000 INFO @ Tue, 30 Jun 2020 00:43:28: 23000000 INFO @ Tue, 30 Jun 2020 00:43:32: 19000000 INFO @ Tue, 30 Jun 2020 00:43:32: 12000000 INFO @ Tue, 30 Jun 2020 00:43:35: 24000000 INFO @ Tue, 30 Jun 2020 00:43:38: 20000000 INFO @ Tue, 30 Jun 2020 00:43:39: 13000000 INFO @ Tue, 30 Jun 2020 00:43:42: 25000000 INFO @ Tue, 30 Jun 2020 00:43:43: #1 tag size is determined as 74 bps INFO @ Tue, 30 Jun 2020 00:43:43: #1 tag size = 74 INFO @ Tue, 30 Jun 2020 00:43:43: #1 total tags in treatment: 25209912 INFO @ Tue, 30 Jun 2020 00:43:43: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:43:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:43:44: #1 tags after filtering in treatment: 25209912 INFO @ Tue, 30 Jun 2020 00:43:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:43:44: #1 finished! INFO @ Tue, 30 Jun 2020 00:43:44: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:43:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:43:45: 21000000 INFO @ Tue, 30 Jun 2020 00:43:45: #2 number of paired peaks: 10 WARNING @ Tue, 30 Jun 2020 00:43:45: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:43:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:43:46: 14000000 INFO @ Tue, 30 Jun 2020 00:43:51: 22000000 INFO @ Tue, 30 Jun 2020 00:43:54: 15000000 INFO @ Tue, 30 Jun 2020 00:43:58: 23000000 INFO @ Tue, 30 Jun 2020 00:44:01: 16000000 INFO @ Tue, 30 Jun 2020 00:44:04: 24000000 INFO @ Tue, 30 Jun 2020 00:44:08: 17000000 INFO @ Tue, 30 Jun 2020 00:44:11: 25000000 INFO @ Tue, 30 Jun 2020 00:44:13: #1 tag size is determined as 74 bps INFO @ Tue, 30 Jun 2020 00:44:13: #1 tag size = 74 INFO @ Tue, 30 Jun 2020 00:44:13: #1 total tags in treatment: 25209912 INFO @ Tue, 30 Jun 2020 00:44:13: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:44:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:44:13: #1 tags after filtering in treatment: 25209912 INFO @ Tue, 30 Jun 2020 00:44:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:44:13: #1 finished! INFO @ Tue, 30 Jun 2020 00:44:13: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:44:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:44:15: #2 number of paired peaks: 10 WARNING @ Tue, 30 Jun 2020 00:44:15: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:44:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:44:16: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:44:23: 19000000 INFO @ Tue, 30 Jun 2020 00:44:30: 20000000 INFO @ Tue, 30 Jun 2020 00:44:37: 21000000 INFO @ Tue, 30 Jun 2020 00:44:44: 22000000 INFO @ Tue, 30 Jun 2020 00:44:51: 23000000 INFO @ Tue, 30 Jun 2020 00:44:58: 24000000 INFO @ Tue, 30 Jun 2020 00:45:05: 25000000 INFO @ Tue, 30 Jun 2020 00:45:06: #1 tag size is determined as 74 bps INFO @ Tue, 30 Jun 2020 00:45:06: #1 tag size = 74 INFO @ Tue, 30 Jun 2020 00:45:06: #1 total tags in treatment: 25209912 INFO @ Tue, 30 Jun 2020 00:45:06: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:45:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:45:07: #1 tags after filtering in treatment: 25209912 INFO @ Tue, 30 Jun 2020 00:45:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:45:07: #1 finished! INFO @ Tue, 30 Jun 2020 00:45:07: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:45:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:45:08: #2 number of paired peaks: 10 WARNING @ Tue, 30 Jun 2020 00:45:08: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:45:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287832/SRX5287832.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。