Job ID = 6528297 SRX = SRX5287830 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:38:28 prefetch.2.10.7: 1) Downloading 'SRR8483061'... 2020-06-29T14:38:28 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:40:58 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:40:58 prefetch.2.10.7: 1) 'SRR8483061' was downloaded successfully 2020-06-29T14:40:58 prefetch.2.10.7: 'SRR8483061' has 0 unresolved dependencies Read 20530272 spots for SRR8483061/SRR8483061.sra Written 20530272 spots for SRR8483061/SRR8483061.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:48 20530272 reads; of these: 20530272 (100.00%) were unpaired; of these: 856226 (4.17%) aligned 0 times 14324244 (69.77%) aligned exactly 1 time 5349802 (26.06%) aligned >1 times 95.83% overall alignment rate Time searching: 00:08:48 Overall time: 00:08:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2334472 / 19674046 = 0.1187 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:02:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:02:51: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:02:51: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:02:57: 1000000 INFO @ Tue, 30 Jun 2020 00:03:03: 2000000 INFO @ Tue, 30 Jun 2020 00:03:09: 3000000 INFO @ Tue, 30 Jun 2020 00:03:15: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:03:20: 5000000 INFO @ Tue, 30 Jun 2020 00:03:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:03:21: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:03:21: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:03:27: 6000000 INFO @ Tue, 30 Jun 2020 00:03:29: 1000000 INFO @ Tue, 30 Jun 2020 00:03:35: 7000000 INFO @ Tue, 30 Jun 2020 00:03:37: 2000000 INFO @ Tue, 30 Jun 2020 00:03:42: 8000000 INFO @ Tue, 30 Jun 2020 00:03:46: 3000000 INFO @ Tue, 30 Jun 2020 00:03:49: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:03:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:03:51: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:03:51: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:03:54: 4000000 INFO @ Tue, 30 Jun 2020 00:03:56: 10000000 INFO @ Tue, 30 Jun 2020 00:03:58: 1000000 INFO @ Tue, 30 Jun 2020 00:04:02: 5000000 INFO @ Tue, 30 Jun 2020 00:04:04: 11000000 INFO @ Tue, 30 Jun 2020 00:04:06: 2000000 INFO @ Tue, 30 Jun 2020 00:04:10: 6000000 INFO @ Tue, 30 Jun 2020 00:04:11: 12000000 INFO @ Tue, 30 Jun 2020 00:04:13: 3000000 INFO @ Tue, 30 Jun 2020 00:04:18: 13000000 INFO @ Tue, 30 Jun 2020 00:04:18: 7000000 INFO @ Tue, 30 Jun 2020 00:04:20: 4000000 INFO @ Tue, 30 Jun 2020 00:04:25: 14000000 INFO @ Tue, 30 Jun 2020 00:04:27: 8000000 INFO @ Tue, 30 Jun 2020 00:04:27: 5000000 INFO @ Tue, 30 Jun 2020 00:04:33: 15000000 INFO @ Tue, 30 Jun 2020 00:04:34: 6000000 INFO @ Tue, 30 Jun 2020 00:04:35: 9000000 INFO @ Tue, 30 Jun 2020 00:04:40: 16000000 INFO @ Tue, 30 Jun 2020 00:04:42: 7000000 INFO @ Tue, 30 Jun 2020 00:04:43: 10000000 INFO @ Tue, 30 Jun 2020 00:04:47: 17000000 INFO @ Tue, 30 Jun 2020 00:04:49: 8000000 INFO @ Tue, 30 Jun 2020 00:04:50: #1 tag size is determined as 74 bps INFO @ Tue, 30 Jun 2020 00:04:50: #1 tag size = 74 INFO @ Tue, 30 Jun 2020 00:04:50: #1 total tags in treatment: 17339574 INFO @ Tue, 30 Jun 2020 00:04:50: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:04:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:04:50: #1 tags after filtering in treatment: 17339574 INFO @ Tue, 30 Jun 2020 00:04:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:04:50: #1 finished! INFO @ Tue, 30 Jun 2020 00:04:50: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:04:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:04:51: 11000000 INFO @ Tue, 30 Jun 2020 00:04:51: #2 number of paired peaks: 23 WARNING @ Tue, 30 Jun 2020 00:04:51: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:04:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:04:56: 9000000 INFO @ Tue, 30 Jun 2020 00:04:59: 12000000 INFO @ Tue, 30 Jun 2020 00:05:03: 10000000 INFO @ Tue, 30 Jun 2020 00:05:07: 13000000 INFO @ Tue, 30 Jun 2020 00:05:10: 11000000 INFO @ Tue, 30 Jun 2020 00:05:15: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:05:18: 12000000 INFO @ Tue, 30 Jun 2020 00:05:24: 15000000 INFO @ Tue, 30 Jun 2020 00:05:25: 13000000 INFO @ Tue, 30 Jun 2020 00:05:32: 16000000 INFO @ Tue, 30 Jun 2020 00:05:32: 14000000 INFO @ Tue, 30 Jun 2020 00:05:39: 15000000 INFO @ Tue, 30 Jun 2020 00:05:40: 17000000 INFO @ Tue, 30 Jun 2020 00:05:43: #1 tag size is determined as 74 bps INFO @ Tue, 30 Jun 2020 00:05:43: #1 tag size = 74 INFO @ Tue, 30 Jun 2020 00:05:43: #1 total tags in treatment: 17339574 INFO @ Tue, 30 Jun 2020 00:05:43: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:05:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:05:43: #1 tags after filtering in treatment: 17339574 INFO @ Tue, 30 Jun 2020 00:05:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:05:43: #1 finished! INFO @ Tue, 30 Jun 2020 00:05:43: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:05:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:05:44: #2 number of paired peaks: 23 WARNING @ Tue, 30 Jun 2020 00:05:44: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:05:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:05:46: 16000000 INFO @ Tue, 30 Jun 2020 00:05:53: 17000000 BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 00:05:55: #1 tag size is determined as 74 bps INFO @ Tue, 30 Jun 2020 00:05:55: #1 tag size = 74 INFO @ Tue, 30 Jun 2020 00:05:55: #1 total tags in treatment: 17339574 INFO @ Tue, 30 Jun 2020 00:05:55: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:05:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:05:56: #1 tags after filtering in treatment: 17339574 INFO @ Tue, 30 Jun 2020 00:05:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:05:56: #1 finished! INFO @ Tue, 30 Jun 2020 00:05:56: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:05:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:05:57: #2 number of paired peaks: 23 WARNING @ Tue, 30 Jun 2020 00:05:57: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:05:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287830/SRX5287830.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling