Job ID = 6528291 SRX = SRX5287826 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:42:43 prefetch.2.10.7: 1) Downloading 'SRR8483057'... 2020-06-29T14:42:43 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:45:35 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:45:35 prefetch.2.10.7: 1) 'SRR8483057' was downloaded successfully 2020-06-29T14:45:35 prefetch.2.10.7: 'SRR8483057' has 0 unresolved dependencies Read 24665534 spots for SRR8483057/SRR8483057.sra Written 24665534 spots for SRR8483057/SRR8483057.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:56 24665534 reads; of these: 24665534 (100.00%) were unpaired; of these: 1100260 (4.46%) aligned 0 times 16934427 (68.66%) aligned exactly 1 time 6630847 (26.88%) aligned >1 times 95.54% overall alignment rate Time searching: 00:11:57 Overall time: 00:11:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2902326 / 23565274 = 0.1232 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:14:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:14:36: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:14:36: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:14:44: 1000000 INFO @ Tue, 30 Jun 2020 00:14:53: 2000000 INFO @ Tue, 30 Jun 2020 00:15:01: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:15:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:15:06: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:15:06: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:15:10: 4000000 INFO @ Tue, 30 Jun 2020 00:15:15: 1000000 INFO @ Tue, 30 Jun 2020 00:15:19: 5000000 INFO @ Tue, 30 Jun 2020 00:15:25: 2000000 INFO @ Tue, 30 Jun 2020 00:15:28: 6000000 INFO @ Tue, 30 Jun 2020 00:15:34: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:15:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:15:36: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:15:36: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:15:37: 7000000 INFO @ Tue, 30 Jun 2020 00:15:43: 4000000 INFO @ Tue, 30 Jun 2020 00:15:45: 1000000 INFO @ Tue, 30 Jun 2020 00:15:46: 8000000 INFO @ Tue, 30 Jun 2020 00:15:53: 5000000 INFO @ Tue, 30 Jun 2020 00:15:53: 2000000 INFO @ Tue, 30 Jun 2020 00:15:55: 9000000 INFO @ Tue, 30 Jun 2020 00:16:02: 3000000 INFO @ Tue, 30 Jun 2020 00:16:02: 6000000 INFO @ Tue, 30 Jun 2020 00:16:04: 10000000 INFO @ Tue, 30 Jun 2020 00:16:11: 4000000 INFO @ Tue, 30 Jun 2020 00:16:11: 7000000 INFO @ Tue, 30 Jun 2020 00:16:13: 11000000 INFO @ Tue, 30 Jun 2020 00:16:19: 5000000 INFO @ Tue, 30 Jun 2020 00:16:21: 8000000 INFO @ Tue, 30 Jun 2020 00:16:22: 12000000 INFO @ Tue, 30 Jun 2020 00:16:28: 6000000 INFO @ Tue, 30 Jun 2020 00:16:30: 9000000 INFO @ Tue, 30 Jun 2020 00:16:31: 13000000 INFO @ Tue, 30 Jun 2020 00:16:36: 7000000 INFO @ Tue, 30 Jun 2020 00:16:39: 10000000 INFO @ Tue, 30 Jun 2020 00:16:40: 14000000 INFO @ Tue, 30 Jun 2020 00:16:45: 8000000 INFO @ Tue, 30 Jun 2020 00:16:49: 11000000 INFO @ Tue, 30 Jun 2020 00:16:49: 15000000 INFO @ Tue, 30 Jun 2020 00:16:53: 9000000 INFO @ Tue, 30 Jun 2020 00:16:58: 12000000 INFO @ Tue, 30 Jun 2020 00:16:58: 16000000 INFO @ Tue, 30 Jun 2020 00:17:02: 10000000 INFO @ Tue, 30 Jun 2020 00:17:07: 13000000 INFO @ Tue, 30 Jun 2020 00:17:07: 17000000 INFO @ Tue, 30 Jun 2020 00:17:10: 11000000 INFO @ Tue, 30 Jun 2020 00:17:17: 18000000 INFO @ Tue, 30 Jun 2020 00:17:17: 14000000 INFO @ Tue, 30 Jun 2020 00:17:19: 12000000 INFO @ Tue, 30 Jun 2020 00:17:26: 19000000 INFO @ Tue, 30 Jun 2020 00:17:26: 15000000 INFO @ Tue, 30 Jun 2020 00:17:27: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:17:35: 20000000 INFO @ Tue, 30 Jun 2020 00:17:36: 14000000 INFO @ Tue, 30 Jun 2020 00:17:36: 16000000 INFO @ Tue, 30 Jun 2020 00:17:41: #1 tag size is determined as 74 bps INFO @ Tue, 30 Jun 2020 00:17:41: #1 tag size = 74 INFO @ Tue, 30 Jun 2020 00:17:41: #1 total tags in treatment: 20662948 INFO @ Tue, 30 Jun 2020 00:17:41: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:17:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:17:41: #1 tags after filtering in treatment: 20662948 INFO @ Tue, 30 Jun 2020 00:17:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:17:41: #1 finished! INFO @ Tue, 30 Jun 2020 00:17:41: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:17:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:17:43: #2 number of paired peaks: 19 WARNING @ Tue, 30 Jun 2020 00:17:43: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:17:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:17:44: 15000000 INFO @ Tue, 30 Jun 2020 00:17:46: 17000000 INFO @ Tue, 30 Jun 2020 00:17:53: 16000000 INFO @ Tue, 30 Jun 2020 00:17:55: 18000000 INFO @ Tue, 30 Jun 2020 00:18:01: 17000000 INFO @ Tue, 30 Jun 2020 00:18:04: 19000000 INFO @ Tue, 30 Jun 2020 00:18:10: 18000000 BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 00:18:14: 20000000 INFO @ Tue, 30 Jun 2020 00:18:18: 19000000 INFO @ Tue, 30 Jun 2020 00:18:20: #1 tag size is determined as 74 bps INFO @ Tue, 30 Jun 2020 00:18:20: #1 tag size = 74 INFO @ Tue, 30 Jun 2020 00:18:20: #1 total tags in treatment: 20662948 INFO @ Tue, 30 Jun 2020 00:18:20: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:18:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:18:20: #1 tags after filtering in treatment: 20662948 INFO @ Tue, 30 Jun 2020 00:18:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:18:20: #1 finished! INFO @ Tue, 30 Jun 2020 00:18:20: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:18:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:18:22: #2 number of paired peaks: 19 WARNING @ Tue, 30 Jun 2020 00:18:22: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:18:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:18:26: 20000000 INFO @ Tue, 30 Jun 2020 00:18:31: #1 tag size is determined as 74 bps INFO @ Tue, 30 Jun 2020 00:18:31: #1 tag size = 74 INFO @ Tue, 30 Jun 2020 00:18:31: #1 total tags in treatment: 20662948 INFO @ Tue, 30 Jun 2020 00:18:31: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:18:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:18:31: #1 tags after filtering in treatment: 20662948 INFO @ Tue, 30 Jun 2020 00:18:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:18:31: #1 finished! INFO @ Tue, 30 Jun 2020 00:18:31: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:18:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:18:33: #2 number of paired peaks: 19 WARNING @ Tue, 30 Jun 2020 00:18:33: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:18:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287826/SRX5287826.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling