Job ID = 3785864 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-11-01T05:33:49 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:33:49 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.24' from '172.19.7.14' 2019-11-01T05:33:49 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.24) from '172.19.7.14' 2019-11-01T05:33:49 fasterq-dump.2.9.6 err: connection failed while opening file within cryptographic module - error with https open 'https://sra-download.ncbi.nlm.nih.gov/traces/sra75/SRR/008242/SRR8440604' 2019-11-01T05:33:49 fasterq-dump.2.9.6 err: invalid accession 'SRR8440604' 2019-11-01T05:35:54 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:35:54 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:37:12 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:25 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:25 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:46 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:49 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:52 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:43:32 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:43:39 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:43:39 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:44:44 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:45:14 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:45:15 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:45:50 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:11 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:37 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:45 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:45 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:47:15 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:47:53 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:47:57 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:48:27 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:48:30 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:48:42 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:49:32 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:49:46 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:50:17 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:50:35 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:52:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:11 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:42 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:55:22 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:55:25 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:55:42 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:57:44 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:57:44 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:57:47 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:58:12 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:59:15 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T06:01:14 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 25,044,003 reads read : 25,044,003 reads written : 25,044,003 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:09 25044003 reads; of these: 25044003 (100.00%) were unpaired; of these: 1313316 (5.24%) aligned 0 times 15691327 (62.66%) aligned exactly 1 time 8039360 (32.10%) aligned >1 times 94.76% overall alignment rate Time searching: 00:09:09 Overall time: 00:09:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 1959413 / 23730687 = 0.0826 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Fri, 01 Nov 2019 15:17:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:17:49: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:17:49: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:17:57: 1000000 INFO @ Fri, 01 Nov 2019 15:18:05: 2000000 INFO @ Fri, 01 Nov 2019 15:18:12: 3000000 INFO @ Fri, 01 Nov 2019 15:18:19: 4000000 INFO @ Fri, 01 Nov 2019 15:18:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:18:19: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:18:19: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:18:27: 5000000 INFO @ Fri, 01 Nov 2019 15:18:28: 1000000 INFO @ Fri, 01 Nov 2019 15:18:34: 6000000 INFO @ Fri, 01 Nov 2019 15:18:36: 2000000 INFO @ Fri, 01 Nov 2019 15:18:42: 7000000 INFO @ Fri, 01 Nov 2019 15:18:44: 3000000 BedGraph に変換中... INFO @ Fri, 01 Nov 2019 15:18:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:18:49: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:18:49: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:18:49: 8000000 INFO @ Fri, 01 Nov 2019 15:18:52: 4000000 INFO @ Fri, 01 Nov 2019 15:18:57: 9000000 INFO @ Fri, 01 Nov 2019 15:18:58: 1000000 INFO @ Fri, 01 Nov 2019 15:19:01: 5000000 INFO @ Fri, 01 Nov 2019 15:19:05: 10000000 INFO @ Fri, 01 Nov 2019 15:19:05: 2000000 INFO @ Fri, 01 Nov 2019 15:19:10: 6000000 INFO @ Fri, 01 Nov 2019 15:19:12: 11000000 INFO @ Fri, 01 Nov 2019 15:19:13: 3000000 INFO @ Fri, 01 Nov 2019 15:19:18: 7000000 INFO @ Fri, 01 Nov 2019 15:19:20: 12000000 INFO @ Fri, 01 Nov 2019 15:19:21: 4000000 INFO @ Fri, 01 Nov 2019 15:19:27: 8000000 INFO @ Fri, 01 Nov 2019 15:19:28: 13000000 INFO @ Fri, 01 Nov 2019 15:19:28: 5000000 INFO @ Fri, 01 Nov 2019 15:19:36: 14000000 INFO @ Fri, 01 Nov 2019 15:19:36: 9000000 INFO @ Fri, 01 Nov 2019 15:19:36: 6000000 INFO @ Fri, 01 Nov 2019 15:19:44: 15000000 INFO @ Fri, 01 Nov 2019 15:19:44: 7000000 INFO @ Fri, 01 Nov 2019 15:19:45: 10000000 INFO @ Fri, 01 Nov 2019 15:19:51: 16000000 INFO @ Fri, 01 Nov 2019 15:19:52: 8000000 INFO @ Fri, 01 Nov 2019 15:19:53: 11000000 INFO @ Fri, 01 Nov 2019 15:19:59: 17000000 INFO @ Fri, 01 Nov 2019 15:20:00: 9000000 INFO @ Fri, 01 Nov 2019 15:20:02: 12000000 INFO @ Fri, 01 Nov 2019 15:20:07: 18000000 INFO @ Fri, 01 Nov 2019 15:20:07: 10000000 INFO @ Fri, 01 Nov 2019 15:20:10: 13000000 INFO @ Fri, 01 Nov 2019 15:20:14: 19000000 INFO @ Fri, 01 Nov 2019 15:20:15: 11000000 INFO @ Fri, 01 Nov 2019 15:20:19: 14000000 INFO @ Fri, 01 Nov 2019 15:20:22: 20000000 INFO @ Fri, 01 Nov 2019 15:20:23: 12000000 INFO @ Fri, 01 Nov 2019 15:20:28: 15000000 INFO @ Fri, 01 Nov 2019 15:20:30: 21000000 INFO @ Fri, 01 Nov 2019 15:20:30: 13000000 INFO @ Fri, 01 Nov 2019 15:20:36: #1 tag size is determined as 50 bps INFO @ Fri, 01 Nov 2019 15:20:36: #1 tag size = 50 INFO @ Fri, 01 Nov 2019 15:20:36: #1 total tags in treatment: 21771274 INFO @ Fri, 01 Nov 2019 15:20:36: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:20:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:20:36: #1 tags after filtering in treatment: 21771274 INFO @ Fri, 01 Nov 2019 15:20:36: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:20:36: #1 finished! INFO @ Fri, 01 Nov 2019 15:20:36: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:20:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:20:37: 16000000 INFO @ Fri, 01 Nov 2019 15:20:38: 14000000 INFO @ Fri, 01 Nov 2019 15:20:38: #2 number of paired peaks: 177 WARNING @ Fri, 01 Nov 2019 15:20:38: Fewer paired peaks (177) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 177 pairs to build model! INFO @ Fri, 01 Nov 2019 15:20:38: start model_add_line... INFO @ Fri, 01 Nov 2019 15:20:38: start X-correlation... INFO @ Fri, 01 Nov 2019 15:20:38: end of X-cor INFO @ Fri, 01 Nov 2019 15:20:38: #2 finished! INFO @ Fri, 01 Nov 2019 15:20:38: #2 predicted fragment length is 60 bps INFO @ Fri, 01 Nov 2019 15:20:38: #2 alternative fragment length(s) may be 3,60 bps INFO @ Fri, 01 Nov 2019 15:20:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.05_model.r WARNING @ Fri, 01 Nov 2019 15:20:38: #2 Since the d (60) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 01 Nov 2019 15:20:38: #2 You may need to consider one of the other alternative d(s): 3,60 WARNING @ Fri, 01 Nov 2019 15:20:38: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 01 Nov 2019 15:20:38: #3 Call peaks... INFO @ Fri, 01 Nov 2019 15:20:38: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 01 Nov 2019 15:20:45: 17000000 INFO @ Fri, 01 Nov 2019 15:20:46: 15000000 INFO @ Fri, 01 Nov 2019 15:20:53: 16000000 INFO @ Fri, 01 Nov 2019 15:20:54: 18000000 INFO @ Fri, 01 Nov 2019 15:21:01: 17000000 INFO @ Fri, 01 Nov 2019 15:21:03: 19000000 INFO @ Fri, 01 Nov 2019 15:21:09: 18000000 INFO @ Fri, 01 Nov 2019 15:21:11: 20000000 INFO @ Fri, 01 Nov 2019 15:21:16: 19000000 INFO @ Fri, 01 Nov 2019 15:21:20: 21000000 INFO @ Fri, 01 Nov 2019 15:21:24: 20000000 INFO @ Fri, 01 Nov 2019 15:21:26: #1 tag size is determined as 50 bps INFO @ Fri, 01 Nov 2019 15:21:26: #1 tag size = 50 INFO @ Fri, 01 Nov 2019 15:21:26: #1 total tags in treatment: 21771274 INFO @ Fri, 01 Nov 2019 15:21:26: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:21:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:21:27: #1 tags after filtering in treatment: 21771274 INFO @ Fri, 01 Nov 2019 15:21:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:21:27: #1 finished! INFO @ Fri, 01 Nov 2019 15:21:27: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:21:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:21:29: #2 number of paired peaks: 177 WARNING @ Fri, 01 Nov 2019 15:21:29: Fewer paired peaks (177) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 177 pairs to build model! INFO @ Fri, 01 Nov 2019 15:21:29: start model_add_line... INFO @ Fri, 01 Nov 2019 15:21:29: start X-correlation... INFO @ Fri, 01 Nov 2019 15:21:29: end of X-cor INFO @ Fri, 01 Nov 2019 15:21:29: #2 finished! INFO @ Fri, 01 Nov 2019 15:21:29: #2 predicted fragment length is 60 bps INFO @ Fri, 01 Nov 2019 15:21:29: #2 alternative fragment length(s) may be 3,60 bps INFO @ Fri, 01 Nov 2019 15:21:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.10_model.r WARNING @ Fri, 01 Nov 2019 15:21:29: #2 Since the d (60) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 01 Nov 2019 15:21:29: #2 You may need to consider one of the other alternative d(s): 3,60 WARNING @ Fri, 01 Nov 2019 15:21:29: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 01 Nov 2019 15:21:29: #3 Call peaks... INFO @ Fri, 01 Nov 2019 15:21:29: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 01 Nov 2019 15:21:31: 21000000 INFO @ Fri, 01 Nov 2019 15:21:33: #3 Call peaks for each chromosome... INFO @ Fri, 01 Nov 2019 15:21:37: #1 tag size is determined as 50 bps INFO @ Fri, 01 Nov 2019 15:21:37: #1 tag size = 50 INFO @ Fri, 01 Nov 2019 15:21:37: #1 total tags in treatment: 21771274 INFO @ Fri, 01 Nov 2019 15:21:37: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:21:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:21:38: #1 tags after filtering in treatment: 21771274 INFO @ Fri, 01 Nov 2019 15:21:38: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:21:38: #1 finished! INFO @ Fri, 01 Nov 2019 15:21:38: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:21:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:21:40: #2 number of paired peaks: 177 WARNING @ Fri, 01 Nov 2019 15:21:40: Fewer paired peaks (177) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 177 pairs to build model! INFO @ Fri, 01 Nov 2019 15:21:40: start model_add_line... INFO @ Fri, 01 Nov 2019 15:21:40: start X-correlation... INFO @ Fri, 01 Nov 2019 15:21:40: end of X-cor INFO @ Fri, 01 Nov 2019 15:21:40: #2 finished! INFO @ Fri, 01 Nov 2019 15:21:40: #2 predicted fragment length is 60 bps INFO @ Fri, 01 Nov 2019 15:21:40: #2 alternative fragment length(s) may be 3,60 bps INFO @ Fri, 01 Nov 2019 15:21:40: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.20_model.r WARNING @ Fri, 01 Nov 2019 15:21:40: #2 Since the d (60) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 01 Nov 2019 15:21:40: #2 You may need to consider one of the other alternative d(s): 3,60 WARNING @ Fri, 01 Nov 2019 15:21:40: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 01 Nov 2019 15:21:40: #3 Call peaks... INFO @ Fri, 01 Nov 2019 15:21:40: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 01 Nov 2019 15:21:59: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.05_peaks.xls INFO @ Fri, 01 Nov 2019 15:21:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.05_peaks.narrowPeak INFO @ Fri, 01 Nov 2019 15:21:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.05_summits.bed INFO @ Fri, 01 Nov 2019 15:21:59: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (3665 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Fri, 01 Nov 2019 15:22:23: #3 Call peaks for each chromosome... INFO @ Fri, 01 Nov 2019 15:22:34: #3 Call peaks for each chromosome... INFO @ Fri, 01 Nov 2019 15:22:49: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.10_peaks.xls INFO @ Fri, 01 Nov 2019 15:22:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.10_peaks.narrowPeak INFO @ Fri, 01 Nov 2019 15:22:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.10_summits.bed INFO @ Fri, 01 Nov 2019 15:22:49: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (1386 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Fri, 01 Nov 2019 15:23:00: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.20_peaks.xls INFO @ Fri, 01 Nov 2019 15:23:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.20_peaks.narrowPeak INFO @ Fri, 01 Nov 2019 15:23:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX5247957/SRX5247957.20_summits.bed INFO @ Fri, 01 Nov 2019 15:23:00: Done! pass1 - making usageList (10 chroms): 1 millis pass2 - checking and writing primary data (366 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。