Job ID = 3785860 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-11-01T05:35:38 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:35:38 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:35:38 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:35:38 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:35:39 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:35:39 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:40:42 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:41:13 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:41:13 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:41:13 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:41:13 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:41:17 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:41:53 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:42:44 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:44:06 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:44:06 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:45:15 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:45:49 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:07 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.27' from '172.19.7.62' 2019-11-01T05:46:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.27) from '172.19.7.62' 2019-11-01T05:46:37 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:47:13 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:47:30 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:48:42 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:48:47 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:50:09 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:50:14 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:50:19 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:50:35 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:51:01 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:51:17 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:52:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:52:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:07 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:07 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.24' from '172.19.7.62' 2019-11-01T05:53:07 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.24) from '172.19.7.62' 2019-11-01T05:53:14 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:15 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:15 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:54:06 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:54:43 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:54:51 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:55:46 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:55:58 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:55:58 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:59:02 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:59:02 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:59:09 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:59:09 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T06:02:40 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T06:04:47 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 36,221,247 reads read : 36,221,247 reads written : 36,221,247 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:02 36221247 reads; of these: 36221247 (100.00%) were unpaired; of these: 2171538 (6.00%) aligned 0 times 28703276 (79.24%) aligned exactly 1 time 5346433 (14.76%) aligned >1 times 94.00% overall alignment rate Time searching: 00:10:02 Overall time: 00:10:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3684177 / 34049709 = 0.1082 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Fri, 01 Nov 2019 15:29:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:29:47: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:29:47: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:29:54: 1000000 INFO @ Fri, 01 Nov 2019 15:30:01: 2000000 INFO @ Fri, 01 Nov 2019 15:30:09: 3000000 INFO @ Fri, 01 Nov 2019 15:30:16: 4000000 INFO @ Fri, 01 Nov 2019 15:30:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:30:17: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:30:17: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:30:24: 5000000 INFO @ Fri, 01 Nov 2019 15:30:25: 1000000 INFO @ Fri, 01 Nov 2019 15:30:31: 6000000 INFO @ Fri, 01 Nov 2019 15:30:32: 2000000 INFO @ Fri, 01 Nov 2019 15:30:38: 7000000 INFO @ Fri, 01 Nov 2019 15:30:40: 3000000 BedGraph に変換中... INFO @ Fri, 01 Nov 2019 15:30:46: 8000000 INFO @ Fri, 01 Nov 2019 15:30:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:30:47: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:30:47: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:30:48: 4000000 INFO @ Fri, 01 Nov 2019 15:30:54: 9000000 INFO @ Fri, 01 Nov 2019 15:30:55: 1000000 INFO @ Fri, 01 Nov 2019 15:30:55: 5000000 INFO @ Fri, 01 Nov 2019 15:31:02: 10000000 INFO @ Fri, 01 Nov 2019 15:31:03: 6000000 INFO @ Fri, 01 Nov 2019 15:31:03: 2000000 INFO @ Fri, 01 Nov 2019 15:31:11: 11000000 INFO @ Fri, 01 Nov 2019 15:31:11: 7000000 INFO @ Fri, 01 Nov 2019 15:31:12: 3000000 INFO @ Fri, 01 Nov 2019 15:31:18: 8000000 INFO @ Fri, 01 Nov 2019 15:31:19: 12000000 INFO @ Fri, 01 Nov 2019 15:31:20: 4000000 INFO @ Fri, 01 Nov 2019 15:31:26: 9000000 INFO @ Fri, 01 Nov 2019 15:31:27: 13000000 INFO @ Fri, 01 Nov 2019 15:31:28: 5000000 INFO @ Fri, 01 Nov 2019 15:31:32: 10000000 INFO @ Fri, 01 Nov 2019 15:31:35: 14000000 INFO @ Fri, 01 Nov 2019 15:31:36: 6000000 INFO @ Fri, 01 Nov 2019 15:31:39: 11000000 INFO @ Fri, 01 Nov 2019 15:31:43: 15000000 INFO @ Fri, 01 Nov 2019 15:31:44: 7000000 INFO @ Fri, 01 Nov 2019 15:31:46: 12000000 INFO @ Fri, 01 Nov 2019 15:31:50: 16000000 INFO @ Fri, 01 Nov 2019 15:31:52: 8000000 INFO @ Fri, 01 Nov 2019 15:31:53: 13000000 INFO @ Fri, 01 Nov 2019 15:31:58: 17000000 INFO @ Fri, 01 Nov 2019 15:31:59: 14000000 INFO @ Fri, 01 Nov 2019 15:32:01: 9000000 INFO @ Fri, 01 Nov 2019 15:32:05: 18000000 INFO @ Fri, 01 Nov 2019 15:32:06: 15000000 INFO @ Fri, 01 Nov 2019 15:32:09: 10000000 INFO @ Fri, 01 Nov 2019 15:32:13: 16000000 INFO @ Fri, 01 Nov 2019 15:32:13: 19000000 INFO @ Fri, 01 Nov 2019 15:32:17: 11000000 INFO @ Fri, 01 Nov 2019 15:32:19: 17000000 INFO @ Fri, 01 Nov 2019 15:32:20: 20000000 INFO @ Fri, 01 Nov 2019 15:32:25: 12000000 INFO @ Fri, 01 Nov 2019 15:32:26: 18000000 INFO @ Fri, 01 Nov 2019 15:32:28: 21000000 INFO @ Fri, 01 Nov 2019 15:32:32: 19000000 INFO @ Fri, 01 Nov 2019 15:32:33: 13000000 INFO @ Fri, 01 Nov 2019 15:32:36: 22000000 INFO @ Fri, 01 Nov 2019 15:32:39: 20000000 INFO @ Fri, 01 Nov 2019 15:32:41: 14000000 INFO @ Fri, 01 Nov 2019 15:32:43: 23000000 INFO @ Fri, 01 Nov 2019 15:32:45: 21000000 INFO @ Fri, 01 Nov 2019 15:32:49: 15000000 INFO @ Fri, 01 Nov 2019 15:32:51: 24000000 INFO @ Fri, 01 Nov 2019 15:32:52: 22000000 INFO @ Fri, 01 Nov 2019 15:32:56: 16000000 INFO @ Fri, 01 Nov 2019 15:32:58: 23000000 INFO @ Fri, 01 Nov 2019 15:32:59: 25000000 INFO @ Fri, 01 Nov 2019 15:33:04: 17000000 INFO @ Fri, 01 Nov 2019 15:33:05: 24000000 INFO @ Fri, 01 Nov 2019 15:33:07: 26000000 INFO @ Fri, 01 Nov 2019 15:33:12: 18000000 INFO @ Fri, 01 Nov 2019 15:33:12: 25000000 INFO @ Fri, 01 Nov 2019 15:33:15: 27000000 INFO @ Fri, 01 Nov 2019 15:33:19: 26000000 INFO @ Fri, 01 Nov 2019 15:33:20: 19000000 INFO @ Fri, 01 Nov 2019 15:33:22: 28000000 INFO @ Fri, 01 Nov 2019 15:33:25: 27000000 INFO @ Fri, 01 Nov 2019 15:33:27: 20000000 INFO @ Fri, 01 Nov 2019 15:33:30: 29000000 INFO @ Fri, 01 Nov 2019 15:33:32: 28000000 INFO @ Fri, 01 Nov 2019 15:33:35: 21000000 INFO @ Fri, 01 Nov 2019 15:33:38: 30000000 INFO @ Fri, 01 Nov 2019 15:33:38: 29000000 INFO @ Fri, 01 Nov 2019 15:33:41: #1 tag size is determined as 50 bps INFO @ Fri, 01 Nov 2019 15:33:41: #1 tag size = 50 INFO @ Fri, 01 Nov 2019 15:33:41: #1 total tags in treatment: 30365532 INFO @ Fri, 01 Nov 2019 15:33:41: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:33:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:33:41: #1 tags after filtering in treatment: 30365532 INFO @ Fri, 01 Nov 2019 15:33:41: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:33:41: #1 finished! INFO @ Fri, 01 Nov 2019 15:33:41: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:33:41: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:33:43: 22000000 INFO @ Fri, 01 Nov 2019 15:33:44: #2 number of paired peaks: 65 WARNING @ Fri, 01 Nov 2019 15:33:44: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 01 Nov 2019 15:33:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 01 Nov 2019 15:33:45: 30000000 INFO @ Fri, 01 Nov 2019 15:33:47: #1 tag size is determined as 50 bps INFO @ Fri, 01 Nov 2019 15:33:47: #1 tag size = 50 INFO @ Fri, 01 Nov 2019 15:33:47: #1 total tags in treatment: 30365532 INFO @ Fri, 01 Nov 2019 15:33:47: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:33:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:33:48: #1 tags after filtering in treatment: 30365532 INFO @ Fri, 01 Nov 2019 15:33:48: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:33:48: #1 finished! INFO @ Fri, 01 Nov 2019 15:33:48: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:33:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:33:50: 23000000 INFO @ Fri, 01 Nov 2019 15:33:51: #2 number of paired peaks: 65 WARNING @ Fri, 01 Nov 2019 15:33:51: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 01 Nov 2019 15:33:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 01 Nov 2019 15:33:57: 24000000 INFO @ Fri, 01 Nov 2019 15:34:05: 25000000 INFO @ Fri, 01 Nov 2019 15:34:12: 26000000 INFO @ Fri, 01 Nov 2019 15:34:19: 27000000 INFO @ Fri, 01 Nov 2019 15:34:26: 28000000 INFO @ Fri, 01 Nov 2019 15:34:33: 29000000 INFO @ Fri, 01 Nov 2019 15:34:40: 30000000 INFO @ Fri, 01 Nov 2019 15:34:43: #1 tag size is determined as 50 bps INFO @ Fri, 01 Nov 2019 15:34:43: #1 tag size = 50 INFO @ Fri, 01 Nov 2019 15:34:43: #1 total tags in treatment: 30365532 INFO @ Fri, 01 Nov 2019 15:34:43: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:34:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:34:44: #1 tags after filtering in treatment: 30365532 INFO @ Fri, 01 Nov 2019 15:34:44: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:34:44: #1 finished! INFO @ Fri, 01 Nov 2019 15:34:44: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:34:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:34:46: #2 number of paired peaks: 65 WARNING @ Fri, 01 Nov 2019 15:34:46: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 01 Nov 2019 15:34:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5247953/SRX5247953.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。