Job ID = 2590880 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 19,651,010 reads read : 39,302,020 reads written : 19,651,010 reads 0-length : 19,651,010 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:56 19651010 reads; of these: 19651010 (100.00%) were unpaired; of these: 459000 (2.34%) aligned 0 times 15345687 (78.09%) aligned exactly 1 time 3846323 (19.57%) aligned >1 times 97.66% overall alignment rate Time searching: 00:09:56 Overall time: 00:09:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 848801 / 19192010 = 0.0442 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 13 Aug 2019 00:12:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 13 Aug 2019 00:12:58: #1 read tag files... INFO @ Tue, 13 Aug 2019 00:12:58: #1 read treatment tags... INFO @ Tue, 13 Aug 2019 00:12:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 13 Aug 2019 00:12:59: #1 read tag files... INFO @ Tue, 13 Aug 2019 00:12:59: #1 read treatment tags... INFO @ Tue, 13 Aug 2019 00:13:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 13 Aug 2019 00:13:00: #1 read tag files... INFO @ Tue, 13 Aug 2019 00:13:00: #1 read treatment tags... INFO @ Tue, 13 Aug 2019 00:13:07: 1000000 INFO @ Tue, 13 Aug 2019 00:13:08: 1000000 INFO @ Tue, 13 Aug 2019 00:13:09: 1000000 INFO @ Tue, 13 Aug 2019 00:13:16: 2000000 INFO @ Tue, 13 Aug 2019 00:13:16: 2000000 INFO @ Tue, 13 Aug 2019 00:13:17: 2000000 INFO @ Tue, 13 Aug 2019 00:13:24: 3000000 INFO @ Tue, 13 Aug 2019 00:13:24: 3000000 INFO @ Tue, 13 Aug 2019 00:13:25: 3000000 INFO @ Tue, 13 Aug 2019 00:13:32: 4000000 INFO @ Tue, 13 Aug 2019 00:13:32: 4000000 INFO @ Tue, 13 Aug 2019 00:13:33: 4000000 INFO @ Tue, 13 Aug 2019 00:13:39: 5000000 INFO @ Tue, 13 Aug 2019 00:13:41: 5000000 INFO @ Tue, 13 Aug 2019 00:13:42: 5000000 INFO @ Tue, 13 Aug 2019 00:13:47: 6000000 INFO @ Tue, 13 Aug 2019 00:13:49: 6000000 INFO @ Tue, 13 Aug 2019 00:13:50: 6000000 INFO @ Tue, 13 Aug 2019 00:13:56: 7000000 INFO @ Tue, 13 Aug 2019 00:13:58: 7000000 INFO @ Tue, 13 Aug 2019 00:13:59: 7000000 INFO @ Tue, 13 Aug 2019 00:14:04: 8000000 INFO @ Tue, 13 Aug 2019 00:14:07: 8000000 INFO @ Tue, 13 Aug 2019 00:14:08: 8000000 INFO @ Tue, 13 Aug 2019 00:14:13: 9000000 INFO @ Tue, 13 Aug 2019 00:14:16: 9000000 INFO @ Tue, 13 Aug 2019 00:14:17: 9000000 INFO @ Tue, 13 Aug 2019 00:14:21: 10000000 INFO @ Tue, 13 Aug 2019 00:14:25: 10000000 INFO @ Tue, 13 Aug 2019 00:14:26: 10000000 INFO @ Tue, 13 Aug 2019 00:14:29: 11000000 INFO @ Tue, 13 Aug 2019 00:14:33: 11000000 INFO @ Tue, 13 Aug 2019 00:14:34: 11000000 INFO @ Tue, 13 Aug 2019 00:14:37: 12000000 INFO @ Tue, 13 Aug 2019 00:14:42: 12000000 INFO @ Tue, 13 Aug 2019 00:14:42: 12000000 INFO @ Tue, 13 Aug 2019 00:14:46: 13000000 INFO @ Tue, 13 Aug 2019 00:14:50: 13000000 INFO @ Tue, 13 Aug 2019 00:14:50: 13000000 INFO @ Tue, 13 Aug 2019 00:14:54: 14000000 INFO @ Tue, 13 Aug 2019 00:14:58: 14000000 INFO @ Tue, 13 Aug 2019 00:14:58: 14000000 INFO @ Tue, 13 Aug 2019 00:15:02: 15000000 INFO @ Tue, 13 Aug 2019 00:15:06: 15000000 INFO @ Tue, 13 Aug 2019 00:15:06: 15000000 INFO @ Tue, 13 Aug 2019 00:15:11: 16000000 INFO @ Tue, 13 Aug 2019 00:15:14: 16000000 INFO @ Tue, 13 Aug 2019 00:15:14: 16000000 INFO @ Tue, 13 Aug 2019 00:15:19: 17000000 INFO @ Tue, 13 Aug 2019 00:15:21: 17000000 INFO @ Tue, 13 Aug 2019 00:15:22: 17000000 INFO @ Tue, 13 Aug 2019 00:15:27: 18000000 INFO @ Tue, 13 Aug 2019 00:15:29: 18000000 INFO @ Tue, 13 Aug 2019 00:15:29: #1 tag size is determined as 76 bps INFO @ Tue, 13 Aug 2019 00:15:29: #1 tag size = 76 INFO @ Tue, 13 Aug 2019 00:15:29: #1 total tags in treatment: 18343209 INFO @ Tue, 13 Aug 2019 00:15:29: #1 user defined the maximum tags... INFO @ Tue, 13 Aug 2019 00:15:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 13 Aug 2019 00:15:30: #1 tags after filtering in treatment: 18343209 INFO @ Tue, 13 Aug 2019 00:15:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 13 Aug 2019 00:15:30: #1 finished! INFO @ Tue, 13 Aug 2019 00:15:30: #2 Build Peak Model... INFO @ Tue, 13 Aug 2019 00:15:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 13 Aug 2019 00:15:30: 18000000 INFO @ Tue, 13 Aug 2019 00:15:31: #2 number of paired peaks: 7 WARNING @ Tue, 13 Aug 2019 00:15:31: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 13 Aug 2019 00:15:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 13 Aug 2019 00:15:32: #1 tag size is determined as 76 bps INFO @ Tue, 13 Aug 2019 00:15:32: #1 tag size = 76 INFO @ Tue, 13 Aug 2019 00:15:32: #1 total tags in treatment: 18343209 INFO @ Tue, 13 Aug 2019 00:15:32: #1 user defined the maximum tags... INFO @ Tue, 13 Aug 2019 00:15:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 13 Aug 2019 00:15:33: #1 tags after filtering in treatment: 18343209 INFO @ Tue, 13 Aug 2019 00:15:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 13 Aug 2019 00:15:33: #1 finished! INFO @ Tue, 13 Aug 2019 00:15:33: #2 Build Peak Model... INFO @ Tue, 13 Aug 2019 00:15:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 13 Aug 2019 00:15:33: #1 tag size is determined as 76 bps INFO @ Tue, 13 Aug 2019 00:15:33: #1 tag size = 76 INFO @ Tue, 13 Aug 2019 00:15:33: #1 total tags in treatment: 18343209 INFO @ Tue, 13 Aug 2019 00:15:33: #1 user defined the maximum tags... INFO @ Tue, 13 Aug 2019 00:15:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 13 Aug 2019 00:15:33: #1 tags after filtering in treatment: 18343209 INFO @ Tue, 13 Aug 2019 00:15:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 13 Aug 2019 00:15:33: #1 finished! INFO @ Tue, 13 Aug 2019 00:15:33: #2 Build Peak Model... INFO @ Tue, 13 Aug 2019 00:15:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 13 Aug 2019 00:15:34: #2 number of paired peaks: 7 WARNING @ Tue, 13 Aug 2019 00:15:34: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 13 Aug 2019 00:15:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 13 Aug 2019 00:15:35: #2 number of paired peaks: 7 WARNING @ Tue, 13 Aug 2019 00:15:35: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 13 Aug 2019 00:15:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125697/SRX5125697.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。