Job ID = 2590879 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 22,962,458 reads read : 45,924,916 reads written : 22,962,458 reads 0-length : 22,962,458 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:56 22962458 reads; of these: 22962458 (100.00%) were unpaired; of these: 567196 (2.47%) aligned 0 times 18274533 (79.58%) aligned exactly 1 time 4120729 (17.95%) aligned >1 times 97.53% overall alignment rate Time searching: 00:09:56 Overall time: 00:09:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 945156 / 22395262 = 0.0422 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 13 Aug 2019 00:14:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 13 Aug 2019 00:14:31: #1 read tag files... INFO @ Tue, 13 Aug 2019 00:14:31: #1 read treatment tags... INFO @ Tue, 13 Aug 2019 00:14:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 13 Aug 2019 00:14:32: #1 read tag files... INFO @ Tue, 13 Aug 2019 00:14:32: #1 read treatment tags... INFO @ Tue, 13 Aug 2019 00:14:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 13 Aug 2019 00:14:33: #1 read tag files... INFO @ Tue, 13 Aug 2019 00:14:33: #1 read treatment tags... INFO @ Tue, 13 Aug 2019 00:14:41: 1000000 INFO @ Tue, 13 Aug 2019 00:14:41: 1000000 INFO @ Tue, 13 Aug 2019 00:14:43: 1000000 INFO @ Tue, 13 Aug 2019 00:14:49: 2000000 INFO @ Tue, 13 Aug 2019 00:14:50: 2000000 INFO @ Tue, 13 Aug 2019 00:14:53: 2000000 INFO @ Tue, 13 Aug 2019 00:14:58: 3000000 INFO @ Tue, 13 Aug 2019 00:15:00: 3000000 INFO @ Tue, 13 Aug 2019 00:15:02: 3000000 INFO @ Tue, 13 Aug 2019 00:15:06: 4000000 INFO @ Tue, 13 Aug 2019 00:15:08: 4000000 INFO @ Tue, 13 Aug 2019 00:15:13: 4000000 INFO @ Tue, 13 Aug 2019 00:15:15: 5000000 INFO @ Tue, 13 Aug 2019 00:15:17: 5000000 INFO @ Tue, 13 Aug 2019 00:15:23: 6000000 INFO @ Tue, 13 Aug 2019 00:15:23: 5000000 INFO @ Tue, 13 Aug 2019 00:15:26: 6000000 INFO @ Tue, 13 Aug 2019 00:15:31: 7000000 INFO @ Tue, 13 Aug 2019 00:15:33: 6000000 INFO @ Tue, 13 Aug 2019 00:15:34: 7000000 INFO @ Tue, 13 Aug 2019 00:15:40: 8000000 INFO @ Tue, 13 Aug 2019 00:15:43: 8000000 INFO @ Tue, 13 Aug 2019 00:15:43: 7000000 INFO @ Tue, 13 Aug 2019 00:15:48: 9000000 INFO @ Tue, 13 Aug 2019 00:15:51: 9000000 INFO @ Tue, 13 Aug 2019 00:15:53: 8000000 INFO @ Tue, 13 Aug 2019 00:15:57: 10000000 INFO @ Tue, 13 Aug 2019 00:15:59: 10000000 INFO @ Tue, 13 Aug 2019 00:16:02: 9000000 INFO @ Tue, 13 Aug 2019 00:16:05: 11000000 INFO @ Tue, 13 Aug 2019 00:16:07: 11000000 INFO @ Tue, 13 Aug 2019 00:16:12: 10000000 INFO @ Tue, 13 Aug 2019 00:16:13: 12000000 INFO @ Tue, 13 Aug 2019 00:16:16: 12000000 INFO @ Tue, 13 Aug 2019 00:16:22: 13000000 INFO @ Tue, 13 Aug 2019 00:16:22: 11000000 INFO @ Tue, 13 Aug 2019 00:16:24: 13000000 INFO @ Tue, 13 Aug 2019 00:16:30: 14000000 INFO @ Tue, 13 Aug 2019 00:16:31: 12000000 INFO @ Tue, 13 Aug 2019 00:16:32: 14000000 INFO @ Tue, 13 Aug 2019 00:16:38: 15000000 INFO @ Tue, 13 Aug 2019 00:16:40: 15000000 INFO @ Tue, 13 Aug 2019 00:16:41: 13000000 INFO @ Tue, 13 Aug 2019 00:16:47: 16000000 INFO @ Tue, 13 Aug 2019 00:16:49: 16000000 INFO @ Tue, 13 Aug 2019 00:16:51: 14000000 INFO @ Tue, 13 Aug 2019 00:16:55: 17000000 INFO @ Tue, 13 Aug 2019 00:16:57: 17000000 INFO @ Tue, 13 Aug 2019 00:17:00: 15000000 INFO @ Tue, 13 Aug 2019 00:17:03: 18000000 INFO @ Tue, 13 Aug 2019 00:17:05: 18000000 INFO @ Tue, 13 Aug 2019 00:17:10: 16000000 INFO @ Tue, 13 Aug 2019 00:17:12: 19000000 INFO @ Tue, 13 Aug 2019 00:17:14: 19000000 INFO @ Tue, 13 Aug 2019 00:17:20: 17000000 INFO @ Tue, 13 Aug 2019 00:17:20: 20000000 INFO @ Tue, 13 Aug 2019 00:17:22: 20000000 INFO @ Tue, 13 Aug 2019 00:17:28: 21000000 INFO @ Tue, 13 Aug 2019 00:17:30: 18000000 INFO @ Tue, 13 Aug 2019 00:17:30: 21000000 INFO @ Tue, 13 Aug 2019 00:17:32: #1 tag size is determined as 76 bps INFO @ Tue, 13 Aug 2019 00:17:32: #1 tag size = 76 INFO @ Tue, 13 Aug 2019 00:17:32: #1 total tags in treatment: 21450106 INFO @ Tue, 13 Aug 2019 00:17:32: #1 user defined the maximum tags... INFO @ Tue, 13 Aug 2019 00:17:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 13 Aug 2019 00:17:33: #1 tags after filtering in treatment: 21450106 INFO @ Tue, 13 Aug 2019 00:17:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 13 Aug 2019 00:17:33: #1 finished! INFO @ Tue, 13 Aug 2019 00:17:33: #2 Build Peak Model... INFO @ Tue, 13 Aug 2019 00:17:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 13 Aug 2019 00:17:34: #1 tag size is determined as 76 bps INFO @ Tue, 13 Aug 2019 00:17:34: #1 tag size = 76 INFO @ Tue, 13 Aug 2019 00:17:34: #1 total tags in treatment: 21450106 INFO @ Tue, 13 Aug 2019 00:17:34: #1 user defined the maximum tags... INFO @ Tue, 13 Aug 2019 00:17:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 13 Aug 2019 00:17:34: #1 tags after filtering in treatment: 21450106 INFO @ Tue, 13 Aug 2019 00:17:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 13 Aug 2019 00:17:34: #1 finished! INFO @ Tue, 13 Aug 2019 00:17:34: #2 Build Peak Model... INFO @ Tue, 13 Aug 2019 00:17:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 13 Aug 2019 00:17:35: #2 number of paired peaks: 1 WARNING @ Tue, 13 Aug 2019 00:17:35: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 13 Aug 2019 00:17:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 13 Aug 2019 00:17:36: #2 number of paired peaks: 1 WARNING @ Tue, 13 Aug 2019 00:17:36: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 13 Aug 2019 00:17:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 13 Aug 2019 00:17:39: 19000000 INFO @ Tue, 13 Aug 2019 00:17:49: 20000000 INFO @ Tue, 13 Aug 2019 00:17:58: 21000000 INFO @ Tue, 13 Aug 2019 00:18:02: #1 tag size is determined as 76 bps INFO @ Tue, 13 Aug 2019 00:18:02: #1 tag size = 76 INFO @ Tue, 13 Aug 2019 00:18:02: #1 total tags in treatment: 21450106 INFO @ Tue, 13 Aug 2019 00:18:02: #1 user defined the maximum tags... INFO @ Tue, 13 Aug 2019 00:18:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 13 Aug 2019 00:18:03: #1 tags after filtering in treatment: 21450106 INFO @ Tue, 13 Aug 2019 00:18:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 13 Aug 2019 00:18:03: #1 finished! INFO @ Tue, 13 Aug 2019 00:18:03: #2 Build Peak Model... INFO @ Tue, 13 Aug 2019 00:18:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 13 Aug 2019 00:18:05: #2 number of paired peaks: 1 WARNING @ Tue, 13 Aug 2019 00:18:05: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 13 Aug 2019 00:18:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125696/SRX5125696.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。