Job ID = 2590877 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 16,416,905 reads read : 32,833,810 reads written : 16,416,905 reads 0-length : 16,416,905 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:37 16416905 reads; of these: 16416905 (100.00%) were unpaired; of these: 1611935 (9.82%) aligned 0 times 12651307 (77.06%) aligned exactly 1 time 2153663 (13.12%) aligned >1 times 90.18% overall alignment rate Time searching: 00:06:37 Overall time: 00:06:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 808807 / 14804970 = 0.0546 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 13 Aug 2019 00:06:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 13 Aug 2019 00:06:11: #1 read tag files... INFO @ Tue, 13 Aug 2019 00:06:11: #1 read treatment tags... INFO @ Tue, 13 Aug 2019 00:06:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 13 Aug 2019 00:06:12: #1 read tag files... INFO @ Tue, 13 Aug 2019 00:06:12: #1 read treatment tags... INFO @ Tue, 13 Aug 2019 00:06:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 13 Aug 2019 00:06:13: #1 read tag files... INFO @ Tue, 13 Aug 2019 00:06:13: #1 read treatment tags... INFO @ Tue, 13 Aug 2019 00:06:19: 1000000 INFO @ Tue, 13 Aug 2019 00:06:20: 1000000 INFO @ Tue, 13 Aug 2019 00:06:24: 1000000 INFO @ Tue, 13 Aug 2019 00:06:27: 2000000 INFO @ Tue, 13 Aug 2019 00:06:28: 2000000 INFO @ Tue, 13 Aug 2019 00:06:34: 3000000 INFO @ Tue, 13 Aug 2019 00:06:35: 2000000 INFO @ Tue, 13 Aug 2019 00:06:35: 3000000 INFO @ Tue, 13 Aug 2019 00:06:42: 4000000 INFO @ Tue, 13 Aug 2019 00:06:43: 4000000 INFO @ Tue, 13 Aug 2019 00:06:46: 3000000 INFO @ Tue, 13 Aug 2019 00:06:50: 5000000 INFO @ Tue, 13 Aug 2019 00:06:51: 5000000 INFO @ Tue, 13 Aug 2019 00:06:57: 4000000 INFO @ Tue, 13 Aug 2019 00:06:58: 6000000 INFO @ Tue, 13 Aug 2019 00:06:59: 6000000 INFO @ Tue, 13 Aug 2019 00:07:06: 7000000 INFO @ Tue, 13 Aug 2019 00:07:07: 7000000 INFO @ Tue, 13 Aug 2019 00:07:08: 5000000 INFO @ Tue, 13 Aug 2019 00:07:13: 8000000 INFO @ Tue, 13 Aug 2019 00:07:15: 8000000 INFO @ Tue, 13 Aug 2019 00:07:19: 6000000 INFO @ Tue, 13 Aug 2019 00:07:21: 9000000 INFO @ Tue, 13 Aug 2019 00:07:22: 9000000 INFO @ Tue, 13 Aug 2019 00:07:29: 10000000 INFO @ Tue, 13 Aug 2019 00:07:30: 10000000 INFO @ Tue, 13 Aug 2019 00:07:31: 7000000 INFO @ Tue, 13 Aug 2019 00:07:37: 11000000 INFO @ Tue, 13 Aug 2019 00:07:38: 11000000 INFO @ Tue, 13 Aug 2019 00:07:42: 8000000 INFO @ Tue, 13 Aug 2019 00:07:45: 12000000 INFO @ Tue, 13 Aug 2019 00:07:46: 12000000 INFO @ Tue, 13 Aug 2019 00:07:52: 9000000 INFO @ Tue, 13 Aug 2019 00:07:52: 13000000 INFO @ Tue, 13 Aug 2019 00:07:54: 13000000 INFO @ Tue, 13 Aug 2019 00:08:00: #1 tag size is determined as 76 bps INFO @ Tue, 13 Aug 2019 00:08:00: #1 tag size = 76 INFO @ Tue, 13 Aug 2019 00:08:00: #1 total tags in treatment: 13996163 INFO @ Tue, 13 Aug 2019 00:08:00: #1 user defined the maximum tags... INFO @ Tue, 13 Aug 2019 00:08:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 13 Aug 2019 00:08:01: #1 tags after filtering in treatment: 13996163 INFO @ Tue, 13 Aug 2019 00:08:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 13 Aug 2019 00:08:01: #1 finished! INFO @ Tue, 13 Aug 2019 00:08:01: #2 Build Peak Model... INFO @ Tue, 13 Aug 2019 00:08:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 13 Aug 2019 00:08:02: #1 tag size is determined as 76 bps INFO @ Tue, 13 Aug 2019 00:08:02: #1 tag size = 76 INFO @ Tue, 13 Aug 2019 00:08:02: #1 total tags in treatment: 13996163 INFO @ Tue, 13 Aug 2019 00:08:02: #1 user defined the maximum tags... INFO @ Tue, 13 Aug 2019 00:08:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 13 Aug 2019 00:08:02: #2 number of paired peaks: 54 WARNING @ Tue, 13 Aug 2019 00:08:02: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 13 Aug 2019 00:08:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 13 Aug 2019 00:08:02: #1 tags after filtering in treatment: 13996163 INFO @ Tue, 13 Aug 2019 00:08:02: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 13 Aug 2019 00:08:02: #1 finished! INFO @ Tue, 13 Aug 2019 00:08:02: #2 Build Peak Model... INFO @ Tue, 13 Aug 2019 00:08:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 13 Aug 2019 00:08:03: #2 number of paired peaks: 54 WARNING @ Tue, 13 Aug 2019 00:08:03: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 13 Aug 2019 00:08:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) INFO @ Tue, 13 Aug 2019 00:08:03: 10000000 rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 13 Aug 2019 00:08:14: 11000000 INFO @ Tue, 13 Aug 2019 00:08:25: 12000000 INFO @ Tue, 13 Aug 2019 00:08:35: 13000000 INFO @ Tue, 13 Aug 2019 00:08:46: #1 tag size is determined as 76 bps INFO @ Tue, 13 Aug 2019 00:08:46: #1 tag size = 76 INFO @ Tue, 13 Aug 2019 00:08:46: #1 total tags in treatment: 13996163 INFO @ Tue, 13 Aug 2019 00:08:46: #1 user defined the maximum tags... INFO @ Tue, 13 Aug 2019 00:08:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 13 Aug 2019 00:08:46: #1 tags after filtering in treatment: 13996163 INFO @ Tue, 13 Aug 2019 00:08:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 13 Aug 2019 00:08:46: #1 finished! INFO @ Tue, 13 Aug 2019 00:08:46: #2 Build Peak Model... INFO @ Tue, 13 Aug 2019 00:08:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 13 Aug 2019 00:08:47: #2 number of paired peaks: 54 WARNING @ Tue, 13 Aug 2019 00:08:47: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 13 Aug 2019 00:08:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5125694/SRX5125694.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。