Job ID = 12265265 SRX = SRX5010725 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 113325609 spots for SRR8191150/SRR8191150.sra Written 113325609 spots for SRR8191150/SRR8191150.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265541 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:34:15 113325609 reads; of these: 113325609 (100.00%) were unpaired; of these: 2407135 (2.12%) aligned 0 times 64819289 (57.20%) aligned exactly 1 time 46099185 (40.68%) aligned >1 times 97.88% overall alignment rate Time searching: 00:34:15 Overall time: 00:34:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 48 files... [bam_rmdupse_core] 67838736 / 110918474 = 0.6116 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:22:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:22:12: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:22:12: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:22:19: 1000000 INFO @ Sat, 03 Apr 2021 07:22:26: 2000000 INFO @ Sat, 03 Apr 2021 07:22:33: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:22:40: 4000000 INFO @ Sat, 03 Apr 2021 07:22:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:22:42: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:22:42: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:22:47: 5000000 INFO @ Sat, 03 Apr 2021 07:22:50: 1000000 INFO @ Sat, 03 Apr 2021 07:22:54: 6000000 INFO @ Sat, 03 Apr 2021 07:22:57: 2000000 INFO @ Sat, 03 Apr 2021 07:23:01: 7000000 INFO @ Sat, 03 Apr 2021 07:23:04: 3000000 INFO @ Sat, 03 Apr 2021 07:23:07: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:23:11: 4000000 INFO @ Sat, 03 Apr 2021 07:23:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:23:12: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:23:12: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:23:14: 9000000 INFO @ Sat, 03 Apr 2021 07:23:18: 5000000 INFO @ Sat, 03 Apr 2021 07:23:19: 1000000 INFO @ Sat, 03 Apr 2021 07:23:21: 10000000 INFO @ Sat, 03 Apr 2021 07:23:25: 6000000 INFO @ Sat, 03 Apr 2021 07:23:26: 2000000 INFO @ Sat, 03 Apr 2021 07:23:28: 11000000 INFO @ Sat, 03 Apr 2021 07:23:32: 7000000 INFO @ Sat, 03 Apr 2021 07:23:33: 3000000 INFO @ Sat, 03 Apr 2021 07:23:35: 12000000 INFO @ Sat, 03 Apr 2021 07:23:39: 8000000 INFO @ Sat, 03 Apr 2021 07:23:40: 4000000 INFO @ Sat, 03 Apr 2021 07:23:41: 13000000 INFO @ Sat, 03 Apr 2021 07:23:46: 9000000 INFO @ Sat, 03 Apr 2021 07:23:47: 5000000 INFO @ Sat, 03 Apr 2021 07:23:48: 14000000 INFO @ Sat, 03 Apr 2021 07:23:53: 10000000 INFO @ Sat, 03 Apr 2021 07:23:55: 15000000 INFO @ Sat, 03 Apr 2021 07:23:55: 6000000 INFO @ Sat, 03 Apr 2021 07:24:00: 11000000 INFO @ Sat, 03 Apr 2021 07:24:02: 16000000 INFO @ Sat, 03 Apr 2021 07:24:03: 7000000 INFO @ Sat, 03 Apr 2021 07:24:07: 12000000 INFO @ Sat, 03 Apr 2021 07:24:08: 17000000 INFO @ Sat, 03 Apr 2021 07:24:11: 8000000 INFO @ Sat, 03 Apr 2021 07:24:14: 13000000 INFO @ Sat, 03 Apr 2021 07:24:15: 18000000 INFO @ Sat, 03 Apr 2021 07:24:18: 9000000 INFO @ Sat, 03 Apr 2021 07:24:21: 14000000 INFO @ Sat, 03 Apr 2021 07:24:22: 19000000 INFO @ Sat, 03 Apr 2021 07:24:25: 10000000 INFO @ Sat, 03 Apr 2021 07:24:28: 15000000 INFO @ Sat, 03 Apr 2021 07:24:29: 20000000 INFO @ Sat, 03 Apr 2021 07:24:32: 11000000 INFO @ Sat, 03 Apr 2021 07:24:35: 16000000 INFO @ Sat, 03 Apr 2021 07:24:36: 21000000 INFO @ Sat, 03 Apr 2021 07:24:39: 12000000 INFO @ Sat, 03 Apr 2021 07:24:41: 17000000 INFO @ Sat, 03 Apr 2021 07:24:42: 22000000 INFO @ Sat, 03 Apr 2021 07:24:46: 13000000 INFO @ Sat, 03 Apr 2021 07:24:48: 18000000 INFO @ Sat, 03 Apr 2021 07:24:49: 23000000 INFO @ Sat, 03 Apr 2021 07:24:53: 14000000 INFO @ Sat, 03 Apr 2021 07:24:55: 19000000 INFO @ Sat, 03 Apr 2021 07:24:56: 24000000 INFO @ Sat, 03 Apr 2021 07:24:59: 15000000 INFO @ Sat, 03 Apr 2021 07:25:02: 20000000 INFO @ Sat, 03 Apr 2021 07:25:03: 25000000 INFO @ Sat, 03 Apr 2021 07:25:06: 16000000 INFO @ Sat, 03 Apr 2021 07:25:08: 21000000 INFO @ Sat, 03 Apr 2021 07:25:10: 26000000 INFO @ Sat, 03 Apr 2021 07:25:13: 17000000 INFO @ Sat, 03 Apr 2021 07:25:15: 22000000 INFO @ Sat, 03 Apr 2021 07:25:17: 27000000 INFO @ Sat, 03 Apr 2021 07:25:20: 18000000 INFO @ Sat, 03 Apr 2021 07:25:22: 23000000 INFO @ Sat, 03 Apr 2021 07:25:25: 28000000 INFO @ Sat, 03 Apr 2021 07:25:26: 19000000 INFO @ Sat, 03 Apr 2021 07:25:29: 24000000 INFO @ Sat, 03 Apr 2021 07:25:32: 29000000 INFO @ Sat, 03 Apr 2021 07:25:33: 20000000 INFO @ Sat, 03 Apr 2021 07:25:36: 25000000 INFO @ Sat, 03 Apr 2021 07:25:38: 30000000 INFO @ Sat, 03 Apr 2021 07:25:40: 21000000 INFO @ Sat, 03 Apr 2021 07:25:44: 26000000 INFO @ Sat, 03 Apr 2021 07:25:45: 31000000 INFO @ Sat, 03 Apr 2021 07:25:47: 22000000 INFO @ Sat, 03 Apr 2021 07:25:52: 27000000 INFO @ Sat, 03 Apr 2021 07:25:52: 32000000 INFO @ Sat, 03 Apr 2021 07:25:54: 23000000 INFO @ Sat, 03 Apr 2021 07:25:59: 33000000 INFO @ Sat, 03 Apr 2021 07:25:59: 28000000 INFO @ Sat, 03 Apr 2021 07:26:00: 24000000 INFO @ Sat, 03 Apr 2021 07:26:05: 34000000 INFO @ Sat, 03 Apr 2021 07:26:06: 29000000 INFO @ Sat, 03 Apr 2021 07:26:07: 25000000 INFO @ Sat, 03 Apr 2021 07:26:12: 35000000 INFO @ Sat, 03 Apr 2021 07:26:12: 30000000 INFO @ Sat, 03 Apr 2021 07:26:13: 26000000 INFO @ Sat, 03 Apr 2021 07:26:18: 36000000 INFO @ Sat, 03 Apr 2021 07:26:19: 31000000 INFO @ Sat, 03 Apr 2021 07:26:20: 27000000 INFO @ Sat, 03 Apr 2021 07:26:25: 37000000 INFO @ Sat, 03 Apr 2021 07:26:25: 32000000 INFO @ Sat, 03 Apr 2021 07:26:26: 28000000 INFO @ Sat, 03 Apr 2021 07:26:31: 38000000 INFO @ Sat, 03 Apr 2021 07:26:32: 33000000 INFO @ Sat, 03 Apr 2021 07:26:33: 29000000 INFO @ Sat, 03 Apr 2021 07:26:38: 39000000 INFO @ Sat, 03 Apr 2021 07:26:38: 34000000 INFO @ Sat, 03 Apr 2021 07:26:39: 30000000 INFO @ Sat, 03 Apr 2021 07:26:44: 40000000 INFO @ Sat, 03 Apr 2021 07:26:44: 35000000 INFO @ Sat, 03 Apr 2021 07:26:46: 31000000 INFO @ Sat, 03 Apr 2021 07:26:50: 41000000 INFO @ Sat, 03 Apr 2021 07:26:50: 36000000 INFO @ Sat, 03 Apr 2021 07:26:52: 32000000 INFO @ Sat, 03 Apr 2021 07:26:56: 42000000 INFO @ Sat, 03 Apr 2021 07:26:57: 37000000 INFO @ Sat, 03 Apr 2021 07:26:58: 33000000 INFO @ Sat, 03 Apr 2021 07:27:03: 43000000 INFO @ Sat, 03 Apr 2021 07:27:03: 38000000 INFO @ Sat, 03 Apr 2021 07:27:04: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:27:04: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:27:04: #1 total tags in treatment: 43079738 INFO @ Sat, 03 Apr 2021 07:27:04: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:27:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:27:05: 34000000 INFO @ Sat, 03 Apr 2021 07:27:05: #1 tags after filtering in treatment: 43079738 INFO @ Sat, 03 Apr 2021 07:27:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 07:27:05: #1 finished! INFO @ Sat, 03 Apr 2021 07:27:05: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:27:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:27:07: #2 number of paired peaks: 13 WARNING @ Sat, 03 Apr 2021 07:27:07: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:27:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:27:10: 39000000 INFO @ Sat, 03 Apr 2021 07:27:11: 35000000 INFO @ Sat, 03 Apr 2021 07:27:16: 40000000 INFO @ Sat, 03 Apr 2021 07:27:17: 36000000 INFO @ Sat, 03 Apr 2021 07:27:22: 41000000 INFO @ Sat, 03 Apr 2021 07:27:23: 37000000 INFO @ Sat, 03 Apr 2021 07:27:28: 42000000 INFO @ Sat, 03 Apr 2021 07:27:29: 38000000 INFO @ Sat, 03 Apr 2021 07:27:34: 43000000 INFO @ Sat, 03 Apr 2021 07:27:35: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:27:35: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:27:35: #1 total tags in treatment: 43079738 INFO @ Sat, 03 Apr 2021 07:27:35: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:27:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:27:35: 39000000 INFO @ Sat, 03 Apr 2021 07:27:36: #1 tags after filtering in treatment: 43079738 INFO @ Sat, 03 Apr 2021 07:27:36: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 07:27:36: #1 finished! INFO @ Sat, 03 Apr 2021 07:27:36: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:27:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:27:39: #2 number of paired peaks: 13 WARNING @ Sat, 03 Apr 2021 07:27:39: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:27:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:27:41: 40000000 INFO @ Sat, 03 Apr 2021 07:27:47: 41000000 INFO @ Sat, 03 Apr 2021 07:27:52: 42000000 INFO @ Sat, 03 Apr 2021 07:27:58: 43000000 INFO @ Sat, 03 Apr 2021 07:27:59: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:27:59: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:27:59: #1 total tags in treatment: 43079738 INFO @ Sat, 03 Apr 2021 07:27:59: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:27:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:28:00: #1 tags after filtering in treatment: 43079738 INFO @ Sat, 03 Apr 2021 07:28:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 07:28:00: #1 finished! INFO @ Sat, 03 Apr 2021 07:28:00: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:28:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:28:03: #2 number of paired peaks: 13 WARNING @ Sat, 03 Apr 2021 07:28:03: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:28:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5010725/SRX5010725.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。