Job ID = 12265264 SRX = SRX5010724 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 112702038 spots for SRR8191149/SRR8191149.sra Written 112702038 spots for SRR8191149/SRR8191149.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265519 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:29:52 112702038 reads; of these: 112702038 (100.00%) were unpaired; of these: 2809871 (2.49%) aligned 0 times 72719424 (64.52%) aligned exactly 1 time 37172743 (32.98%) aligned >1 times 97.51% overall alignment rate Time searching: 00:29:52 Overall time: 00:29:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 48 files... [bam_rmdupse_core] 70499610 / 109892167 = 0.6415 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:11:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:11:20: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:11:20: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:11:25: 1000000 INFO @ Sat, 03 Apr 2021 07:11:30: 2000000 INFO @ Sat, 03 Apr 2021 07:11:35: 3000000 INFO @ Sat, 03 Apr 2021 07:11:40: 4000000 INFO @ Sat, 03 Apr 2021 07:11:45: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:11:50: 6000000 INFO @ Sat, 03 Apr 2021 07:11:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:11:50: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:11:50: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:11:55: 1000000 INFO @ Sat, 03 Apr 2021 07:11:55: 7000000 INFO @ Sat, 03 Apr 2021 07:12:00: 2000000 INFO @ Sat, 03 Apr 2021 07:12:00: 8000000 INFO @ Sat, 03 Apr 2021 07:12:05: 3000000 INFO @ Sat, 03 Apr 2021 07:12:05: 9000000 INFO @ Sat, 03 Apr 2021 07:12:11: 4000000 INFO @ Sat, 03 Apr 2021 07:12:11: 10000000 INFO @ Sat, 03 Apr 2021 07:12:16: 5000000 INFO @ Sat, 03 Apr 2021 07:12:16: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:12:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:12:20: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:12:20: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:12:21: 12000000 INFO @ Sat, 03 Apr 2021 07:12:21: 6000000 INFO @ Sat, 03 Apr 2021 07:12:25: 1000000 INFO @ Sat, 03 Apr 2021 07:12:26: 13000000 INFO @ Sat, 03 Apr 2021 07:12:27: 7000000 INFO @ Sat, 03 Apr 2021 07:12:31: 2000000 INFO @ Sat, 03 Apr 2021 07:12:32: 14000000 INFO @ Sat, 03 Apr 2021 07:12:32: 8000000 INFO @ Sat, 03 Apr 2021 07:12:36: 3000000 INFO @ Sat, 03 Apr 2021 07:12:37: 15000000 INFO @ Sat, 03 Apr 2021 07:12:37: 9000000 INFO @ Sat, 03 Apr 2021 07:12:42: 4000000 INFO @ Sat, 03 Apr 2021 07:12:42: 16000000 INFO @ Sat, 03 Apr 2021 07:12:43: 10000000 INFO @ Sat, 03 Apr 2021 07:12:47: 5000000 INFO @ Sat, 03 Apr 2021 07:12:47: 17000000 INFO @ Sat, 03 Apr 2021 07:12:49: 11000000 INFO @ Sat, 03 Apr 2021 07:12:52: 18000000 INFO @ Sat, 03 Apr 2021 07:12:53: 6000000 INFO @ Sat, 03 Apr 2021 07:12:54: 12000000 INFO @ Sat, 03 Apr 2021 07:12:58: 19000000 INFO @ Sat, 03 Apr 2021 07:12:58: 7000000 INFO @ Sat, 03 Apr 2021 07:12:59: 13000000 INFO @ Sat, 03 Apr 2021 07:13:03: 20000000 INFO @ Sat, 03 Apr 2021 07:13:03: 8000000 INFO @ Sat, 03 Apr 2021 07:13:05: 14000000 INFO @ Sat, 03 Apr 2021 07:13:08: 21000000 INFO @ Sat, 03 Apr 2021 07:13:09: 9000000 INFO @ Sat, 03 Apr 2021 07:13:10: 15000000 INFO @ Sat, 03 Apr 2021 07:13:13: 22000000 INFO @ Sat, 03 Apr 2021 07:13:14: 10000000 INFO @ Sat, 03 Apr 2021 07:13:16: 16000000 INFO @ Sat, 03 Apr 2021 07:13:19: 23000000 INFO @ Sat, 03 Apr 2021 07:13:20: 11000000 INFO @ Sat, 03 Apr 2021 07:13:21: 17000000 INFO @ Sat, 03 Apr 2021 07:13:24: 24000000 INFO @ Sat, 03 Apr 2021 07:13:25: 12000000 INFO @ Sat, 03 Apr 2021 07:13:27: 18000000 INFO @ Sat, 03 Apr 2021 07:13:29: 25000000 INFO @ Sat, 03 Apr 2021 07:13:31: 13000000 INFO @ Sat, 03 Apr 2021 07:13:32: 19000000 INFO @ Sat, 03 Apr 2021 07:13:35: 26000000 INFO @ Sat, 03 Apr 2021 07:13:36: 14000000 INFO @ Sat, 03 Apr 2021 07:13:38: 20000000 INFO @ Sat, 03 Apr 2021 07:13:40: 27000000 INFO @ Sat, 03 Apr 2021 07:13:42: 15000000 INFO @ Sat, 03 Apr 2021 07:13:43: 21000000 INFO @ Sat, 03 Apr 2021 07:13:45: 28000000 INFO @ Sat, 03 Apr 2021 07:13:47: 16000000 INFO @ Sat, 03 Apr 2021 07:13:48: 22000000 INFO @ Sat, 03 Apr 2021 07:13:51: 29000000 INFO @ Sat, 03 Apr 2021 07:13:53: 17000000 INFO @ Sat, 03 Apr 2021 07:13:54: 23000000 INFO @ Sat, 03 Apr 2021 07:13:56: 30000000 INFO @ Sat, 03 Apr 2021 07:13:58: 18000000 INFO @ Sat, 03 Apr 2021 07:14:00: 24000000 INFO @ Sat, 03 Apr 2021 07:14:01: 31000000 INFO @ Sat, 03 Apr 2021 07:14:04: 19000000 INFO @ Sat, 03 Apr 2021 07:14:05: 25000000 INFO @ Sat, 03 Apr 2021 07:14:07: 32000000 INFO @ Sat, 03 Apr 2021 07:14:09: 20000000 INFO @ Sat, 03 Apr 2021 07:14:11: 26000000 INFO @ Sat, 03 Apr 2021 07:14:12: 33000000 INFO @ Sat, 03 Apr 2021 07:14:15: 21000000 INFO @ Sat, 03 Apr 2021 07:14:16: 27000000 INFO @ Sat, 03 Apr 2021 07:14:17: 34000000 INFO @ Sat, 03 Apr 2021 07:14:20: 22000000 INFO @ Sat, 03 Apr 2021 07:14:22: 28000000 INFO @ Sat, 03 Apr 2021 07:14:22: 35000000 INFO @ Sat, 03 Apr 2021 07:14:26: 23000000 INFO @ Sat, 03 Apr 2021 07:14:27: 36000000 INFO @ Sat, 03 Apr 2021 07:14:28: 29000000 INFO @ Sat, 03 Apr 2021 07:14:32: 24000000 INFO @ Sat, 03 Apr 2021 07:14:33: 37000000 INFO @ Sat, 03 Apr 2021 07:14:33: 30000000 INFO @ Sat, 03 Apr 2021 07:14:37: 25000000 INFO @ Sat, 03 Apr 2021 07:14:38: 38000000 INFO @ Sat, 03 Apr 2021 07:14:39: 31000000 INFO @ Sat, 03 Apr 2021 07:14:43: 26000000 INFO @ Sat, 03 Apr 2021 07:14:43: 39000000 INFO @ Sat, 03 Apr 2021 07:14:44: 32000000 INFO @ Sat, 03 Apr 2021 07:14:45: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:14:45: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:14:45: #1 total tags in treatment: 39392557 INFO @ Sat, 03 Apr 2021 07:14:45: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:14:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:14:46: #1 tags after filtering in treatment: 39392557 INFO @ Sat, 03 Apr 2021 07:14:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 07:14:46: #1 finished! INFO @ Sat, 03 Apr 2021 07:14:46: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:14:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:14:48: 27000000 INFO @ Sat, 03 Apr 2021 07:14:49: #2 number of paired peaks: 43 WARNING @ Sat, 03 Apr 2021 07:14:49: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:14:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:14:50: 33000000 INFO @ Sat, 03 Apr 2021 07:14:53: 28000000 INFO @ Sat, 03 Apr 2021 07:14:55: 34000000 INFO @ Sat, 03 Apr 2021 07:14:59: 29000000 INFO @ Sat, 03 Apr 2021 07:15:00: 35000000 INFO @ Sat, 03 Apr 2021 07:15:04: 30000000 INFO @ Sat, 03 Apr 2021 07:15:05: 36000000 INFO @ Sat, 03 Apr 2021 07:15:09: 31000000 INFO @ Sat, 03 Apr 2021 07:15:10: 37000000 INFO @ Sat, 03 Apr 2021 07:15:15: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:15:15: 38000000 INFO @ Sat, 03 Apr 2021 07:15:20: 33000000 INFO @ Sat, 03 Apr 2021 07:15:20: 39000000 INFO @ Sat, 03 Apr 2021 07:15:23: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:15:23: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:15:23: #1 total tags in treatment: 39392557 INFO @ Sat, 03 Apr 2021 07:15:23: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:15:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:15:23: #1 tags after filtering in treatment: 39392557 INFO @ Sat, 03 Apr 2021 07:15:23: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 07:15:23: #1 finished! INFO @ Sat, 03 Apr 2021 07:15:23: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:15:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:15:25: 34000000 INFO @ Sat, 03 Apr 2021 07:15:26: #2 number of paired peaks: 43 WARNING @ Sat, 03 Apr 2021 07:15:26: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:15:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:15:30: 35000000 INFO @ Sat, 03 Apr 2021 07:15:34: 36000000 INFO @ Sat, 03 Apr 2021 07:15:39: 37000000 INFO @ Sat, 03 Apr 2021 07:15:44: 38000000 INFO @ Sat, 03 Apr 2021 07:15:49: 39000000 INFO @ Sat, 03 Apr 2021 07:15:51: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:15:51: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:15:51: #1 total tags in treatment: 39392557 INFO @ Sat, 03 Apr 2021 07:15:51: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:15:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:15:52: #1 tags after filtering in treatment: 39392557 INFO @ Sat, 03 Apr 2021 07:15:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 07:15:52: #1 finished! INFO @ Sat, 03 Apr 2021 07:15:52: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:15:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:15:55: #2 number of paired peaks: 43 WARNING @ Sat, 03 Apr 2021 07:15:55: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:15:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5010724/SRX5010724.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。