Job ID = 2590644 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 56,147,132 reads read : 56,147,132 reads written : 56,147,132 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:54 56147132 reads; of these: 56147132 (100.00%) were unpaired; of these: 12208408 (21.74%) aligned 0 times 36778273 (65.50%) aligned exactly 1 time 7160451 (12.75%) aligned >1 times 78.26% overall alignment rate Time searching: 00:14:54 Overall time: 00:14:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 16582128 / 43938724 = 0.3774 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 23:45:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:45:25: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:45:25: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:45:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:45:26: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:45:26: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:45:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:45:27: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:45:27: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:45:34: 1000000 INFO @ Mon, 12 Aug 2019 23:45:38: 1000000 INFO @ Mon, 12 Aug 2019 23:45:39: 1000000 INFO @ Mon, 12 Aug 2019 23:45:43: 2000000 INFO @ Mon, 12 Aug 2019 23:45:50: 2000000 INFO @ Mon, 12 Aug 2019 23:45:51: 2000000 INFO @ Mon, 12 Aug 2019 23:45:52: 3000000 INFO @ Mon, 12 Aug 2019 23:46:01: 4000000 INFO @ Mon, 12 Aug 2019 23:46:02: 3000000 INFO @ Mon, 12 Aug 2019 23:46:03: 3000000 INFO @ Mon, 12 Aug 2019 23:46:10: 5000000 INFO @ Mon, 12 Aug 2019 23:46:14: 4000000 INFO @ Mon, 12 Aug 2019 23:46:16: 4000000 INFO @ Mon, 12 Aug 2019 23:46:19: 6000000 INFO @ Mon, 12 Aug 2019 23:46:26: 5000000 INFO @ Mon, 12 Aug 2019 23:46:28: 7000000 INFO @ Mon, 12 Aug 2019 23:46:29: 5000000 INFO @ Mon, 12 Aug 2019 23:46:36: 8000000 INFO @ Mon, 12 Aug 2019 23:46:39: 6000000 INFO @ Mon, 12 Aug 2019 23:46:41: 6000000 INFO @ Mon, 12 Aug 2019 23:46:45: 9000000 INFO @ Mon, 12 Aug 2019 23:46:52: 7000000 INFO @ Mon, 12 Aug 2019 23:46:54: 10000000 INFO @ Mon, 12 Aug 2019 23:46:54: 7000000 INFO @ Mon, 12 Aug 2019 23:47:03: 11000000 INFO @ Mon, 12 Aug 2019 23:47:04: 8000000 INFO @ Mon, 12 Aug 2019 23:47:07: 8000000 INFO @ Mon, 12 Aug 2019 23:47:11: 12000000 INFO @ Mon, 12 Aug 2019 23:47:17: 9000000 INFO @ Mon, 12 Aug 2019 23:47:19: 9000000 INFO @ Mon, 12 Aug 2019 23:47:20: 13000000 INFO @ Mon, 12 Aug 2019 23:47:30: 10000000 INFO @ Mon, 12 Aug 2019 23:47:30: 14000000 INFO @ Mon, 12 Aug 2019 23:47:32: 10000000 INFO @ Mon, 12 Aug 2019 23:47:39: 15000000 INFO @ Mon, 12 Aug 2019 23:47:42: 11000000 INFO @ Mon, 12 Aug 2019 23:47:44: 11000000 INFO @ Mon, 12 Aug 2019 23:47:48: 16000000 INFO @ Mon, 12 Aug 2019 23:47:55: 12000000 INFO @ Mon, 12 Aug 2019 23:47:57: 12000000 INFO @ Mon, 12 Aug 2019 23:47:58: 17000000 INFO @ Mon, 12 Aug 2019 23:48:06: 13000000 INFO @ Mon, 12 Aug 2019 23:48:08: 18000000 INFO @ Mon, 12 Aug 2019 23:48:09: 13000000 INFO @ Mon, 12 Aug 2019 23:48:18: 19000000 INFO @ Mon, 12 Aug 2019 23:48:19: 14000000 INFO @ Mon, 12 Aug 2019 23:48:23: 14000000 INFO @ Mon, 12 Aug 2019 23:48:27: 20000000 INFO @ Mon, 12 Aug 2019 23:48:32: 15000000 INFO @ Mon, 12 Aug 2019 23:48:36: 15000000 INFO @ Mon, 12 Aug 2019 23:48:36: 21000000 INFO @ Mon, 12 Aug 2019 23:48:45: 16000000 INFO @ Mon, 12 Aug 2019 23:48:46: 22000000 INFO @ Mon, 12 Aug 2019 23:48:49: 16000000 INFO @ Mon, 12 Aug 2019 23:48:56: 23000000 INFO @ Mon, 12 Aug 2019 23:48:58: 17000000 INFO @ Mon, 12 Aug 2019 23:49:02: 17000000 INFO @ Mon, 12 Aug 2019 23:49:05: 24000000 INFO @ Mon, 12 Aug 2019 23:49:12: 18000000 INFO @ Mon, 12 Aug 2019 23:49:14: 25000000 INFO @ Mon, 12 Aug 2019 23:49:15: 18000000 INFO @ Mon, 12 Aug 2019 23:49:24: 26000000 INFO @ Mon, 12 Aug 2019 23:49:25: 19000000 INFO @ Mon, 12 Aug 2019 23:49:28: 19000000 INFO @ Mon, 12 Aug 2019 23:49:33: 27000000 INFO @ Mon, 12 Aug 2019 23:49:36: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:49:36: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:49:36: #1 total tags in treatment: 27356596 INFO @ Mon, 12 Aug 2019 23:49:36: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:49:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:49:37: #1 tags after filtering in treatment: 27356596 INFO @ Mon, 12 Aug 2019 23:49:37: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:49:37: #1 finished! INFO @ Mon, 12 Aug 2019 23:49:37: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:49:37: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:49:37: 20000000 INFO @ Mon, 12 Aug 2019 23:49:39: #2 number of paired peaks: 4 WARNING @ Mon, 12 Aug 2019 23:49:39: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:49:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 23:49:40: 20000000 INFO @ Mon, 12 Aug 2019 23:49:49: 21000000 INFO @ Mon, 12 Aug 2019 23:49:52: 21000000 INFO @ Mon, 12 Aug 2019 23:50:01: 22000000 INFO @ Mon, 12 Aug 2019 23:50:04: 22000000 INFO @ Mon, 12 Aug 2019 23:50:12: 23000000 INFO @ Mon, 12 Aug 2019 23:50:15: 23000000 INFO @ Mon, 12 Aug 2019 23:50:23: 24000000 INFO @ Mon, 12 Aug 2019 23:50:26: 24000000 INFO @ Mon, 12 Aug 2019 23:50:35: 25000000 INFO @ Mon, 12 Aug 2019 23:50:38: 25000000 INFO @ Mon, 12 Aug 2019 23:50:46: 26000000 INFO @ Mon, 12 Aug 2019 23:50:49: 26000000 INFO @ Mon, 12 Aug 2019 23:50:58: 27000000 INFO @ Mon, 12 Aug 2019 23:51:01: 27000000 INFO @ Mon, 12 Aug 2019 23:51:02: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:51:02: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:51:02: #1 total tags in treatment: 27356596 INFO @ Mon, 12 Aug 2019 23:51:02: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:51:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:51:02: #1 tags after filtering in treatment: 27356596 INFO @ Mon, 12 Aug 2019 23:51:02: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:51:02: #1 finished! INFO @ Mon, 12 Aug 2019 23:51:02: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:51:02: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:51:04: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:51:04: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:51:04: #1 total tags in treatment: 27356596 INFO @ Mon, 12 Aug 2019 23:51:04: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:51:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:51:05: #2 number of paired peaks: 4 WARNING @ Mon, 12 Aug 2019 23:51:05: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:51:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 23:51:05: #1 tags after filtering in treatment: 27356596 INFO @ Mon, 12 Aug 2019 23:51:05: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:51:05: #1 finished! INFO @ Mon, 12 Aug 2019 23:51:05: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:51:05: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:51:07: #2 number of paired peaks: 4 WARNING @ Mon, 12 Aug 2019 23:51:07: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:51:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495779/SRX495779.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。