Job ID = 1305914 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 50,358,398 reads read : 50,358,398 reads written : 50,358,398 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:26 50358398 reads; of these: 50358398 (100.00%) were unpaired; of these: 961378 (1.91%) aligned 0 times 41800684 (83.01%) aligned exactly 1 time 7596336 (15.08%) aligned >1 times 98.09% overall alignment rate Time searching: 00:15:26 Overall time: 00:15:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 14891068 / 49397020 = 0.3015 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 22:18:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 22:18:02: #1 read tag files... INFO @ Mon, 03 Jun 2019 22:18:02: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 22:18:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 22:18:02: #1 read tag files... INFO @ Mon, 03 Jun 2019 22:18:02: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 22:18:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 22:18:02: #1 read tag files... INFO @ Mon, 03 Jun 2019 22:18:02: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 22:18:11: 1000000 INFO @ Mon, 03 Jun 2019 22:18:11: 1000000 INFO @ Mon, 03 Jun 2019 22:18:12: 1000000 INFO @ Mon, 03 Jun 2019 22:18:19: 2000000 INFO @ Mon, 03 Jun 2019 22:18:20: 2000000 INFO @ Mon, 03 Jun 2019 22:18:22: 2000000 INFO @ Mon, 03 Jun 2019 22:18:27: 3000000 INFO @ Mon, 03 Jun 2019 22:18:28: 3000000 INFO @ Mon, 03 Jun 2019 22:18:31: 3000000 INFO @ Mon, 03 Jun 2019 22:18:35: 4000000 INFO @ Mon, 03 Jun 2019 22:18:37: 4000000 INFO @ Mon, 03 Jun 2019 22:18:41: 4000000 INFO @ Mon, 03 Jun 2019 22:18:43: 5000000 INFO @ Mon, 03 Jun 2019 22:18:45: 5000000 INFO @ Mon, 03 Jun 2019 22:18:50: 5000000 INFO @ Mon, 03 Jun 2019 22:18:51: 6000000 INFO @ Mon, 03 Jun 2019 22:18:53: 6000000 INFO @ Mon, 03 Jun 2019 22:19:00: 6000000 INFO @ Mon, 03 Jun 2019 22:19:00: 7000000 INFO @ Mon, 03 Jun 2019 22:19:02: 7000000 INFO @ Mon, 03 Jun 2019 22:19:09: 8000000 INFO @ Mon, 03 Jun 2019 22:19:10: 7000000 INFO @ Mon, 03 Jun 2019 22:19:11: 8000000 INFO @ Mon, 03 Jun 2019 22:19:17: 9000000 INFO @ Mon, 03 Jun 2019 22:19:19: 8000000 INFO @ Mon, 03 Jun 2019 22:19:20: 9000000 INFO @ Mon, 03 Jun 2019 22:19:25: 10000000 INFO @ Mon, 03 Jun 2019 22:19:29: 10000000 INFO @ Mon, 03 Jun 2019 22:19:29: 9000000 INFO @ Mon, 03 Jun 2019 22:19:33: 11000000 INFO @ Mon, 03 Jun 2019 22:19:38: 11000000 INFO @ Mon, 03 Jun 2019 22:19:38: 10000000 INFO @ Mon, 03 Jun 2019 22:19:41: 12000000 INFO @ Mon, 03 Jun 2019 22:19:47: 12000000 INFO @ Mon, 03 Jun 2019 22:19:48: 11000000 INFO @ Mon, 03 Jun 2019 22:19:49: 13000000 INFO @ Mon, 03 Jun 2019 22:19:56: 13000000 INFO @ Mon, 03 Jun 2019 22:19:56: 14000000 INFO @ Mon, 03 Jun 2019 22:19:57: 12000000 INFO @ Mon, 03 Jun 2019 22:20:05: 15000000 INFO @ Mon, 03 Jun 2019 22:20:05: 14000000 INFO @ Mon, 03 Jun 2019 22:20:07: 13000000 INFO @ Mon, 03 Jun 2019 22:20:14: 16000000 INFO @ Mon, 03 Jun 2019 22:20:14: 15000000 INFO @ Mon, 03 Jun 2019 22:20:16: 14000000 INFO @ Mon, 03 Jun 2019 22:20:22: 17000000 INFO @ Mon, 03 Jun 2019 22:20:23: 16000000 INFO @ Mon, 03 Jun 2019 22:20:25: 15000000 INFO @ Mon, 03 Jun 2019 22:20:31: 18000000 INFO @ Mon, 03 Jun 2019 22:20:32: 17000000 INFO @ Mon, 03 Jun 2019 22:20:34: 16000000 INFO @ Mon, 03 Jun 2019 22:20:40: 19000000 INFO @ Mon, 03 Jun 2019 22:20:41: 18000000 INFO @ Mon, 03 Jun 2019 22:20:44: 17000000 INFO @ Mon, 03 Jun 2019 22:20:49: 20000000 INFO @ Mon, 03 Jun 2019 22:20:50: 19000000 INFO @ Mon, 03 Jun 2019 22:20:53: 18000000 INFO @ Mon, 03 Jun 2019 22:20:58: 20000000 INFO @ Mon, 03 Jun 2019 22:20:59: 21000000 INFO @ Mon, 03 Jun 2019 22:21:03: 19000000 INFO @ Mon, 03 Jun 2019 22:21:06: 21000000 INFO @ Mon, 03 Jun 2019 22:21:08: 22000000 INFO @ Mon, 03 Jun 2019 22:21:12: 20000000 INFO @ Mon, 03 Jun 2019 22:21:15: 22000000 INFO @ Mon, 03 Jun 2019 22:21:17: 23000000 INFO @ Mon, 03 Jun 2019 22:21:21: 21000000 INFO @ Mon, 03 Jun 2019 22:21:22: 23000000 INFO @ Mon, 03 Jun 2019 22:21:26: 24000000 INFO @ Mon, 03 Jun 2019 22:21:30: 22000000 INFO @ Mon, 03 Jun 2019 22:21:30: 24000000 INFO @ Mon, 03 Jun 2019 22:21:35: 25000000 INFO @ Mon, 03 Jun 2019 22:21:38: 25000000 INFO @ Mon, 03 Jun 2019 22:21:39: 23000000 INFO @ Mon, 03 Jun 2019 22:21:44: 26000000 INFO @ Mon, 03 Jun 2019 22:21:46: 26000000 INFO @ Mon, 03 Jun 2019 22:21:48: 24000000 INFO @ Mon, 03 Jun 2019 22:21:53: 27000000 INFO @ Mon, 03 Jun 2019 22:21:54: 27000000 INFO @ Mon, 03 Jun 2019 22:21:57: 25000000 INFO @ Mon, 03 Jun 2019 22:22:01: 28000000 INFO @ Mon, 03 Jun 2019 22:22:01: 28000000 INFO @ Mon, 03 Jun 2019 22:22:05: 26000000 INFO @ Mon, 03 Jun 2019 22:22:09: 29000000 INFO @ Mon, 03 Jun 2019 22:22:10: 29000000 INFO @ Mon, 03 Jun 2019 22:22:14: 27000000 INFO @ Mon, 03 Jun 2019 22:22:17: 30000000 INFO @ Mon, 03 Jun 2019 22:22:19: 30000000 INFO @ Mon, 03 Jun 2019 22:22:23: 28000000 INFO @ Mon, 03 Jun 2019 22:22:25: 31000000 INFO @ Mon, 03 Jun 2019 22:22:28: 31000000 INFO @ Mon, 03 Jun 2019 22:22:32: 29000000 INFO @ Mon, 03 Jun 2019 22:22:32: 32000000 INFO @ Mon, 03 Jun 2019 22:22:36: 32000000 INFO @ Mon, 03 Jun 2019 22:22:40: 33000000 INFO @ Mon, 03 Jun 2019 22:22:41: 30000000 INFO @ Mon, 03 Jun 2019 22:22:45: 33000000 INFO @ Mon, 03 Jun 2019 22:22:47: 34000000 INFO @ Mon, 03 Jun 2019 22:22:49: 31000000 INFO @ Mon, 03 Jun 2019 22:22:52: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 22:22:52: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 22:22:52: #1 total tags in treatment: 34505952 INFO @ Mon, 03 Jun 2019 22:22:52: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 22:22:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 22:22:52: #1 tags after filtering in treatment: 34505952 INFO @ Mon, 03 Jun 2019 22:22:52: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 22:22:52: #1 finished! INFO @ Mon, 03 Jun 2019 22:22:52: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 22:22:52: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 22:22:53: 34000000 INFO @ Mon, 03 Jun 2019 22:22:55: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 22:22:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 22:22:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 22:22:58: 32000000 INFO @ Mon, 03 Jun 2019 22:22:58: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 22:22:58: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 22:22:58: #1 total tags in treatment: 34505952 INFO @ Mon, 03 Jun 2019 22:22:58: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 22:22:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 22:22:59: #1 tags after filtering in treatment: 34505952 INFO @ Mon, 03 Jun 2019 22:22:59: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 22:22:59: #1 finished! INFO @ Mon, 03 Jun 2019 22:22:59: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 22:22:59: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 22:23:02: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 22:23:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 22:23:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 22:23:06: 33000000 INFO @ Mon, 03 Jun 2019 22:23:15: 34000000 INFO @ Mon, 03 Jun 2019 22:23:19: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 22:23:19: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 22:23:19: #1 total tags in treatment: 34505952 INFO @ Mon, 03 Jun 2019 22:23:19: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 22:23:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 22:23:20: #1 tags after filtering in treatment: 34505952 INFO @ Mon, 03 Jun 2019 22:23:20: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 22:23:20: #1 finished! INFO @ Mon, 03 Jun 2019 22:23:20: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 22:23:20: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 22:23:23: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 22:23:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 22:23:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495309/SRX495309.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。