Job ID = 1305906 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 55,261,147 reads read : 55,261,147 reads written : 55,261,147 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:26 55261147 reads; of these: 55261147 (100.00%) were unpaired; of these: 1066869 (1.93%) aligned 0 times 45427343 (82.20%) aligned exactly 1 time 8766935 (15.86%) aligned >1 times 98.07% overall alignment rate Time searching: 00:16:26 Overall time: 00:16:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 16999422 / 54194278 = 0.3137 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 22:21:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 22:21:20: #1 read tag files... INFO @ Mon, 03 Jun 2019 22:21:20: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 22:21:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 22:21:20: #1 read tag files... INFO @ Mon, 03 Jun 2019 22:21:20: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 22:21:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 22:21:20: #1 read tag files... INFO @ Mon, 03 Jun 2019 22:21:20: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 22:21:27: 1000000 INFO @ Mon, 03 Jun 2019 22:21:28: 1000000 INFO @ Mon, 03 Jun 2019 22:21:29: 1000000 INFO @ Mon, 03 Jun 2019 22:21:35: 2000000 INFO @ Mon, 03 Jun 2019 22:21:36: 2000000 INFO @ Mon, 03 Jun 2019 22:21:37: 2000000 INFO @ Mon, 03 Jun 2019 22:21:42: 3000000 INFO @ Mon, 03 Jun 2019 22:21:44: 3000000 INFO @ Mon, 03 Jun 2019 22:21:46: 3000000 INFO @ Mon, 03 Jun 2019 22:21:51: 4000000 INFO @ Mon, 03 Jun 2019 22:21:53: 4000000 INFO @ Mon, 03 Jun 2019 22:21:54: 4000000 INFO @ Mon, 03 Jun 2019 22:22:00: 5000000 INFO @ Mon, 03 Jun 2019 22:22:02: 5000000 INFO @ Mon, 03 Jun 2019 22:22:02: 5000000 INFO @ Mon, 03 Jun 2019 22:22:10: 6000000 INFO @ Mon, 03 Jun 2019 22:22:11: 6000000 INFO @ Mon, 03 Jun 2019 22:22:11: 6000000 INFO @ Mon, 03 Jun 2019 22:22:20: 7000000 INFO @ Mon, 03 Jun 2019 22:22:20: 7000000 INFO @ Mon, 03 Jun 2019 22:22:20: 7000000 INFO @ Mon, 03 Jun 2019 22:22:28: 8000000 INFO @ Mon, 03 Jun 2019 22:22:30: 8000000 INFO @ Mon, 03 Jun 2019 22:22:30: 8000000 INFO @ Mon, 03 Jun 2019 22:22:36: 9000000 INFO @ Mon, 03 Jun 2019 22:22:39: 9000000 INFO @ Mon, 03 Jun 2019 22:22:40: 9000000 INFO @ Mon, 03 Jun 2019 22:22:44: 10000000 INFO @ Mon, 03 Jun 2019 22:22:49: 10000000 INFO @ Mon, 03 Jun 2019 22:22:49: 10000000 INFO @ Mon, 03 Jun 2019 22:22:52: 11000000 INFO @ Mon, 03 Jun 2019 22:22:57: 11000000 INFO @ Mon, 03 Jun 2019 22:22:59: 11000000 INFO @ Mon, 03 Jun 2019 22:22:59: 12000000 INFO @ Mon, 03 Jun 2019 22:23:06: 12000000 INFO @ Mon, 03 Jun 2019 22:23:07: 13000000 INFO @ Mon, 03 Jun 2019 22:23:08: 12000000 INFO @ Mon, 03 Jun 2019 22:23:14: 14000000 INFO @ Mon, 03 Jun 2019 22:23:15: 13000000 INFO @ Mon, 03 Jun 2019 22:23:18: 13000000 INFO @ Mon, 03 Jun 2019 22:23:22: 15000000 INFO @ Mon, 03 Jun 2019 22:23:24: 14000000 INFO @ Mon, 03 Jun 2019 22:23:27: 14000000 INFO @ Mon, 03 Jun 2019 22:23:29: 16000000 INFO @ Mon, 03 Jun 2019 22:23:33: 15000000 INFO @ Mon, 03 Jun 2019 22:23:37: 15000000 INFO @ Mon, 03 Jun 2019 22:23:37: 17000000 INFO @ Mon, 03 Jun 2019 22:23:42: 16000000 INFO @ Mon, 03 Jun 2019 22:23:45: 18000000 INFO @ Mon, 03 Jun 2019 22:23:46: 16000000 INFO @ Mon, 03 Jun 2019 22:23:51: 17000000 INFO @ Mon, 03 Jun 2019 22:23:53: 19000000 INFO @ Mon, 03 Jun 2019 22:23:56: 17000000 INFO @ Mon, 03 Jun 2019 22:24:00: 18000000 INFO @ Mon, 03 Jun 2019 22:24:01: 20000000 INFO @ Mon, 03 Jun 2019 22:24:05: 18000000 INFO @ Mon, 03 Jun 2019 22:24:08: 19000000 INFO @ Mon, 03 Jun 2019 22:24:09: 21000000 INFO @ Mon, 03 Jun 2019 22:24:14: 19000000 INFO @ Mon, 03 Jun 2019 22:24:16: 22000000 INFO @ Mon, 03 Jun 2019 22:24:17: 20000000 INFO @ Mon, 03 Jun 2019 22:24:23: 20000000 INFO @ Mon, 03 Jun 2019 22:24:23: 23000000 INFO @ Mon, 03 Jun 2019 22:24:25: 21000000 INFO @ Mon, 03 Jun 2019 22:24:31: 24000000 INFO @ Mon, 03 Jun 2019 22:24:32: 21000000 INFO @ Mon, 03 Jun 2019 22:24:34: 22000000 INFO @ Mon, 03 Jun 2019 22:24:38: 25000000 INFO @ Mon, 03 Jun 2019 22:24:41: 22000000 INFO @ Mon, 03 Jun 2019 22:24:42: 23000000 INFO @ Mon, 03 Jun 2019 22:24:46: 26000000 INFO @ Mon, 03 Jun 2019 22:24:50: 23000000 INFO @ Mon, 03 Jun 2019 22:24:51: 24000000 INFO @ Mon, 03 Jun 2019 22:24:54: 27000000 INFO @ Mon, 03 Jun 2019 22:24:59: 24000000 INFO @ Mon, 03 Jun 2019 22:25:00: 25000000 INFO @ Mon, 03 Jun 2019 22:25:02: 28000000 INFO @ Mon, 03 Jun 2019 22:25:09: 26000000 INFO @ Mon, 03 Jun 2019 22:25:09: 25000000 INFO @ Mon, 03 Jun 2019 22:25:10: 29000000 INFO @ Mon, 03 Jun 2019 22:25:17: 27000000 INFO @ Mon, 03 Jun 2019 22:25:18: 30000000 INFO @ Mon, 03 Jun 2019 22:25:18: 26000000 INFO @ Mon, 03 Jun 2019 22:25:26: 28000000 INFO @ Mon, 03 Jun 2019 22:25:26: 31000000 INFO @ Mon, 03 Jun 2019 22:25:27: 27000000 INFO @ Mon, 03 Jun 2019 22:25:34: 32000000 INFO @ Mon, 03 Jun 2019 22:25:35: 29000000 INFO @ Mon, 03 Jun 2019 22:25:36: 28000000 INFO @ Mon, 03 Jun 2019 22:25:42: 33000000 INFO @ Mon, 03 Jun 2019 22:25:43: 30000000 INFO @ Mon, 03 Jun 2019 22:25:45: 29000000 INFO @ Mon, 03 Jun 2019 22:25:49: 34000000 INFO @ Mon, 03 Jun 2019 22:25:52: 31000000 INFO @ Mon, 03 Jun 2019 22:25:54: 30000000 INFO @ Mon, 03 Jun 2019 22:25:57: 35000000 INFO @ Mon, 03 Jun 2019 22:26:01: 32000000 INFO @ Mon, 03 Jun 2019 22:26:04: 31000000 INFO @ Mon, 03 Jun 2019 22:26:04: 36000000 INFO @ Mon, 03 Jun 2019 22:26:10: 33000000 INFO @ Mon, 03 Jun 2019 22:26:12: 37000000 INFO @ Mon, 03 Jun 2019 22:26:14: 32000000 INFO @ Mon, 03 Jun 2019 22:26:15: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 22:26:15: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 22:26:15: #1 total tags in treatment: 37194856 INFO @ Mon, 03 Jun 2019 22:26:15: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 22:26:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 22:26:16: #1 tags after filtering in treatment: 37194856 INFO @ Mon, 03 Jun 2019 22:26:16: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 22:26:16: #1 finished! INFO @ Mon, 03 Jun 2019 22:26:16: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 22:26:16: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 22:26:19: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 22:26:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 22:26:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 22:26:19: 34000000 INFO @ Mon, 03 Jun 2019 22:26:23: 33000000 INFO @ Mon, 03 Jun 2019 22:26:28: 35000000 INFO @ Mon, 03 Jun 2019 22:26:33: 34000000 INFO @ Mon, 03 Jun 2019 22:26:36: 36000000 INFO @ Mon, 03 Jun 2019 22:26:41: 35000000 INFO @ Mon, 03 Jun 2019 22:26:44: 37000000 INFO @ Mon, 03 Jun 2019 22:26:46: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 22:26:46: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 22:26:46: #1 total tags in treatment: 37194856 INFO @ Mon, 03 Jun 2019 22:26:46: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 22:26:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 22:26:47: #1 tags after filtering in treatment: 37194856 INFO @ Mon, 03 Jun 2019 22:26:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 22:26:47: #1 finished! INFO @ Mon, 03 Jun 2019 22:26:47: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 22:26:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 22:26:50: 36000000 INFO @ Mon, 03 Jun 2019 22:26:50: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 22:26:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 22:26:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 22:26:59: 37000000 INFO @ Mon, 03 Jun 2019 22:27:01: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 22:27:01: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 22:27:01: #1 total tags in treatment: 37194856 INFO @ Mon, 03 Jun 2019 22:27:01: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 22:27:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 22:27:02: #1 tags after filtering in treatment: 37194856 INFO @ Mon, 03 Jun 2019 22:27:02: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 22:27:02: #1 finished! INFO @ Mon, 03 Jun 2019 22:27:02: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 22:27:02: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 22:27:05: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 22:27:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 22:27:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495308/SRX495308.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。