Job ID = 1305785 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 31,586,842 reads read : 31,586,842 reads written : 31,586,842 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:38 31586842 reads; of these: 31586842 (100.00%) were unpaired; of these: 1435560 (4.54%) aligned 0 times 24317998 (76.99%) aligned exactly 1 time 5833284 (18.47%) aligned >1 times 95.46% overall alignment rate Time searching: 00:12:38 Overall time: 00:12:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6852728 / 30151282 = 0.2273 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 21:46:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:46:48: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:46:48: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:46:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:46:48: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:46:48: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:46:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:46:49: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:46:49: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:46:58: 1000000 INFO @ Mon, 03 Jun 2019 21:46:58: 1000000 INFO @ Mon, 03 Jun 2019 21:46:58: 1000000 INFO @ Mon, 03 Jun 2019 21:47:06: 2000000 INFO @ Mon, 03 Jun 2019 21:47:07: 2000000 INFO @ Mon, 03 Jun 2019 21:47:07: 2000000 INFO @ Mon, 03 Jun 2019 21:47:14: 3000000 INFO @ Mon, 03 Jun 2019 21:47:16: 3000000 INFO @ Mon, 03 Jun 2019 21:47:16: 3000000 INFO @ Mon, 03 Jun 2019 21:47:21: 4000000 INFO @ Mon, 03 Jun 2019 21:47:25: 4000000 INFO @ Mon, 03 Jun 2019 21:47:25: 4000000 INFO @ Mon, 03 Jun 2019 21:47:29: 5000000 INFO @ Mon, 03 Jun 2019 21:47:33: 5000000 INFO @ Mon, 03 Jun 2019 21:47:34: 5000000 INFO @ Mon, 03 Jun 2019 21:47:37: 6000000 INFO @ Mon, 03 Jun 2019 21:47:42: 6000000 INFO @ Mon, 03 Jun 2019 21:47:42: 6000000 INFO @ Mon, 03 Jun 2019 21:47:45: 7000000 INFO @ Mon, 03 Jun 2019 21:47:51: 7000000 INFO @ Mon, 03 Jun 2019 21:47:51: 7000000 INFO @ Mon, 03 Jun 2019 21:47:53: 8000000 INFO @ Mon, 03 Jun 2019 21:48:00: 8000000 INFO @ Mon, 03 Jun 2019 21:48:00: 8000000 INFO @ Mon, 03 Jun 2019 21:48:02: 9000000 INFO @ Mon, 03 Jun 2019 21:48:09: 9000000 INFO @ Mon, 03 Jun 2019 21:48:09: 9000000 INFO @ Mon, 03 Jun 2019 21:48:12: 10000000 INFO @ Mon, 03 Jun 2019 21:48:18: 10000000 INFO @ Mon, 03 Jun 2019 21:48:18: 10000000 INFO @ Mon, 03 Jun 2019 21:48:20: 11000000 INFO @ Mon, 03 Jun 2019 21:48:27: 11000000 INFO @ Mon, 03 Jun 2019 21:48:27: 11000000 INFO @ Mon, 03 Jun 2019 21:48:30: 12000000 INFO @ Mon, 03 Jun 2019 21:48:37: 12000000 INFO @ Mon, 03 Jun 2019 21:48:37: 12000000 INFO @ Mon, 03 Jun 2019 21:48:40: 13000000 INFO @ Mon, 03 Jun 2019 21:48:46: 13000000 INFO @ Mon, 03 Jun 2019 21:48:46: 13000000 INFO @ Mon, 03 Jun 2019 21:48:50: 14000000 INFO @ Mon, 03 Jun 2019 21:48:55: 14000000 INFO @ Mon, 03 Jun 2019 21:48:55: 14000000 INFO @ Mon, 03 Jun 2019 21:49:00: 15000000 INFO @ Mon, 03 Jun 2019 21:49:04: 15000000 INFO @ Mon, 03 Jun 2019 21:49:04: 15000000 INFO @ Mon, 03 Jun 2019 21:49:10: 16000000 INFO @ Mon, 03 Jun 2019 21:49:13: 16000000 INFO @ Mon, 03 Jun 2019 21:49:13: 16000000 INFO @ Mon, 03 Jun 2019 21:49:20: 17000000 INFO @ Mon, 03 Jun 2019 21:49:22: 17000000 INFO @ Mon, 03 Jun 2019 21:49:22: 17000000 INFO @ Mon, 03 Jun 2019 21:49:30: 18000000 INFO @ Mon, 03 Jun 2019 21:49:31: 18000000 INFO @ Mon, 03 Jun 2019 21:49:31: 18000000 INFO @ Mon, 03 Jun 2019 21:49:40: 19000000 INFO @ Mon, 03 Jun 2019 21:49:40: 19000000 INFO @ Mon, 03 Jun 2019 21:49:40: 19000000 INFO @ Mon, 03 Jun 2019 21:49:50: 20000000 INFO @ Mon, 03 Jun 2019 21:49:50: 20000000 INFO @ Mon, 03 Jun 2019 21:49:50: 20000000 INFO @ Mon, 03 Jun 2019 21:49:59: 21000000 INFO @ Mon, 03 Jun 2019 21:50:00: 21000000 INFO @ Mon, 03 Jun 2019 21:50:00: 21000000 INFO @ Mon, 03 Jun 2019 21:50:08: 22000000 INFO @ Mon, 03 Jun 2019 21:50:09: 22000000 INFO @ Mon, 03 Jun 2019 21:50:10: 22000000 INFO @ Mon, 03 Jun 2019 21:50:17: 23000000 INFO @ Mon, 03 Jun 2019 21:50:18: 23000000 INFO @ Mon, 03 Jun 2019 21:50:19: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:50:19: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:50:19: #1 total tags in treatment: 23298554 INFO @ Mon, 03 Jun 2019 21:50:19: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:50:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:50:20: 23000000 INFO @ Mon, 03 Jun 2019 21:50:20: #1 tags after filtering in treatment: 23298554 INFO @ Mon, 03 Jun 2019 21:50:20: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:50:20: #1 finished! INFO @ Mon, 03 Jun 2019 21:50:20: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:50:20: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:50:21: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:50:21: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:50:21: #1 total tags in treatment: 23298554 INFO @ Mon, 03 Jun 2019 21:50:21: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:50:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:50:21: #1 tags after filtering in treatment: 23298554 INFO @ Mon, 03 Jun 2019 21:50:21: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:50:21: #1 finished! INFO @ Mon, 03 Jun 2019 21:50:21: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:50:21: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:50:22: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 21:50:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:50:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:50:23: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:50:23: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:50:23: #1 total tags in treatment: 23298554 INFO @ Mon, 03 Jun 2019 21:50:23: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:50:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:50:23: #1 tags after filtering in treatment: 23298554 INFO @ Mon, 03 Jun 2019 21:50:23: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:50:23: #1 finished! INFO @ Mon, 03 Jun 2019 21:50:23: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:50:23: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:50:23: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 21:50:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:50:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:50:25: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 21:50:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:50:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495301/SRX495301.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。