Job ID = 6498435 SRX = SRX495292 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:22:16 prefetch.2.10.7: 1) Downloading 'SRR1198797'... 2020-06-25T23:22:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:25:45 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:25:45 prefetch.2.10.7: 1) 'SRR1198797' was downloaded successfully Read 35336269 spots for SRR1198797/SRR1198797.sra Written 35336269 spots for SRR1198797/SRR1198797.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:32 35336269 reads; of these: 35336269 (100.00%) were unpaired; of these: 5545205 (15.69%) aligned 0 times 23785145 (67.31%) aligned exactly 1 time 6005919 (17.00%) aligned >1 times 84.31% overall alignment rate Time searching: 00:10:32 Overall time: 00:10:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6594488 / 29791064 = 0.2214 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:47:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:47:08: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:47:08: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:47:14: 1000000 INFO @ Fri, 26 Jun 2020 08:47:20: 2000000 INFO @ Fri, 26 Jun 2020 08:47:26: 3000000 INFO @ Fri, 26 Jun 2020 08:47:32: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:47:37: 5000000 INFO @ Fri, 26 Jun 2020 08:47:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:47:38: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:47:38: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:47:43: 6000000 INFO @ Fri, 26 Jun 2020 08:47:45: 1000000 INFO @ Fri, 26 Jun 2020 08:47:49: 7000000 INFO @ Fri, 26 Jun 2020 08:47:52: 2000000 INFO @ Fri, 26 Jun 2020 08:47:55: 8000000 INFO @ Fri, 26 Jun 2020 08:47:59: 3000000 INFO @ Fri, 26 Jun 2020 08:48:01: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:48:07: 4000000 INFO @ Fri, 26 Jun 2020 08:48:07: 10000000 INFO @ Fri, 26 Jun 2020 08:48:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:48:08: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:48:08: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:48:13: 11000000 INFO @ Fri, 26 Jun 2020 08:48:14: 5000000 INFO @ Fri, 26 Jun 2020 08:48:16: 1000000 INFO @ Fri, 26 Jun 2020 08:48:19: 12000000 INFO @ Fri, 26 Jun 2020 08:48:22: 6000000 INFO @ Fri, 26 Jun 2020 08:48:24: 2000000 INFO @ Fri, 26 Jun 2020 08:48:25: 13000000 INFO @ Fri, 26 Jun 2020 08:48:30: 7000000 INFO @ Fri, 26 Jun 2020 08:48:31: 14000000 INFO @ Fri, 26 Jun 2020 08:48:32: 3000000 INFO @ Fri, 26 Jun 2020 08:48:37: 15000000 INFO @ Fri, 26 Jun 2020 08:48:38: 8000000 INFO @ Fri, 26 Jun 2020 08:48:39: 4000000 INFO @ Fri, 26 Jun 2020 08:48:43: 16000000 INFO @ Fri, 26 Jun 2020 08:48:45: 9000000 INFO @ Fri, 26 Jun 2020 08:48:47: 5000000 INFO @ Fri, 26 Jun 2020 08:48:49: 17000000 INFO @ Fri, 26 Jun 2020 08:48:53: 10000000 INFO @ Fri, 26 Jun 2020 08:48:55: 18000000 INFO @ Fri, 26 Jun 2020 08:48:55: 6000000 INFO @ Fri, 26 Jun 2020 08:49:01: 11000000 INFO @ Fri, 26 Jun 2020 08:49:01: 19000000 INFO @ Fri, 26 Jun 2020 08:49:03: 7000000 INFO @ Fri, 26 Jun 2020 08:49:07: 20000000 INFO @ Fri, 26 Jun 2020 08:49:08: 12000000 INFO @ Fri, 26 Jun 2020 08:49:11: 8000000 INFO @ Fri, 26 Jun 2020 08:49:14: 21000000 INFO @ Fri, 26 Jun 2020 08:49:15: 13000000 INFO @ Fri, 26 Jun 2020 08:49:19: 9000000 INFO @ Fri, 26 Jun 2020 08:49:20: 22000000 INFO @ Fri, 26 Jun 2020 08:49:23: 14000000 INFO @ Fri, 26 Jun 2020 08:49:26: 23000000 INFO @ Fri, 26 Jun 2020 08:49:27: 10000000 INFO @ Fri, 26 Jun 2020 08:49:27: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 08:49:27: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 08:49:27: #1 total tags in treatment: 23196576 INFO @ Fri, 26 Jun 2020 08:49:27: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:49:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:49:28: #1 tags after filtering in treatment: 23196576 INFO @ Fri, 26 Jun 2020 08:49:28: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:49:28: #1 finished! INFO @ Fri, 26 Jun 2020 08:49:28: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:49:28: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:49:29: #2 number of paired peaks: 17 WARNING @ Fri, 26 Jun 2020 08:49:29: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:49:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:49:30: 15000000 INFO @ Fri, 26 Jun 2020 08:49:33: 11000000 INFO @ Fri, 26 Jun 2020 08:49:38: 16000000 INFO @ Fri, 26 Jun 2020 08:49:40: 12000000 INFO @ Fri, 26 Jun 2020 08:49:45: 17000000 INFO @ Fri, 26 Jun 2020 08:49:46: 13000000 INFO @ Fri, 26 Jun 2020 08:49:52: 18000000 INFO @ Fri, 26 Jun 2020 08:49:53: 14000000 INFO @ Fri, 26 Jun 2020 08:49:59: 19000000 INFO @ Fri, 26 Jun 2020 08:50:00: 15000000 INFO @ Fri, 26 Jun 2020 08:50:07: 20000000 INFO @ Fri, 26 Jun 2020 08:50:07: 16000000 INFO @ Fri, 26 Jun 2020 08:50:13: 17000000 INFO @ Fri, 26 Jun 2020 08:50:14: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 08:50:20: 18000000 INFO @ Fri, 26 Jun 2020 08:50:21: 22000000 INFO @ Fri, 26 Jun 2020 08:50:27: 19000000 INFO @ Fri, 26 Jun 2020 08:50:28: 23000000 INFO @ Fri, 26 Jun 2020 08:50:30: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 08:50:30: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 08:50:30: #1 total tags in treatment: 23196576 INFO @ Fri, 26 Jun 2020 08:50:30: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:50:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:50:30: #1 tags after filtering in treatment: 23196576 INFO @ Fri, 26 Jun 2020 08:50:30: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:50:30: #1 finished! INFO @ Fri, 26 Jun 2020 08:50:30: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:50:30: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:50:32: #2 number of paired peaks: 17 WARNING @ Fri, 26 Jun 2020 08:50:32: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:50:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:50:33: 20000000 INFO @ Fri, 26 Jun 2020 08:50:39: 21000000 INFO @ Fri, 26 Jun 2020 08:50:46: 22000000 INFO @ Fri, 26 Jun 2020 08:50:52: 23000000 INFO @ Fri, 26 Jun 2020 08:50:54: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 08:50:54: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 08:50:54: #1 total tags in treatment: 23196576 INFO @ Fri, 26 Jun 2020 08:50:54: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:50:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:50:54: #1 tags after filtering in treatment: 23196576 INFO @ Fri, 26 Jun 2020 08:50:54: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:50:54: #1 finished! INFO @ Fri, 26 Jun 2020 08:50:54: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:50:54: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:50:56: #2 number of paired peaks: 17 WARNING @ Fri, 26 Jun 2020 08:50:56: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:50:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495292/SRX495292.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。