Job ID = 6498434 SRX = SRX495291 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:27:32 prefetch.2.10.7: 1) Downloading 'SRR1198796'... 2020-06-25T23:27:32 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:32:10 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:32:10 prefetch.2.10.7: 1) 'SRR1198796' was downloaded successfully Read 35336269 spots for SRR1198796/SRR1198796.sra Written 35336269 spots for SRR1198796/SRR1198796.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:39 35336269 reads; of these: 35336269 (100.00%) were unpaired; of these: 5545058 (15.69%) aligned 0 times 23785226 (67.31%) aligned exactly 1 time 6005985 (17.00%) aligned >1 times 84.31% overall alignment rate Time searching: 00:11:39 Overall time: 00:11:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6594531 / 29791211 = 0.2214 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:53:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:53:41: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:53:41: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:53:48: 1000000 INFO @ Fri, 26 Jun 2020 08:53:54: 2000000 INFO @ Fri, 26 Jun 2020 08:54:01: 3000000 INFO @ Fri, 26 Jun 2020 08:54:08: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:54:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:54:10: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:54:10: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:54:15: 5000000 INFO @ Fri, 26 Jun 2020 08:54:19: 1000000 INFO @ Fri, 26 Jun 2020 08:54:23: 6000000 INFO @ Fri, 26 Jun 2020 08:54:27: 2000000 INFO @ Fri, 26 Jun 2020 08:54:31: 7000000 INFO @ Fri, 26 Jun 2020 08:54:36: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:54:39: 8000000 INFO @ Fri, 26 Jun 2020 08:54:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:54:41: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:54:41: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:54:44: 4000000 INFO @ Fri, 26 Jun 2020 08:54:47: 9000000 INFO @ Fri, 26 Jun 2020 08:54:49: 1000000 INFO @ Fri, 26 Jun 2020 08:54:53: 5000000 INFO @ Fri, 26 Jun 2020 08:54:54: 10000000 INFO @ Fri, 26 Jun 2020 08:54:57: 2000000 INFO @ Fri, 26 Jun 2020 08:55:01: 6000000 INFO @ Fri, 26 Jun 2020 08:55:02: 11000000 INFO @ Fri, 26 Jun 2020 08:55:04: 3000000 INFO @ Fri, 26 Jun 2020 08:55:10: 12000000 INFO @ Fri, 26 Jun 2020 08:55:10: 7000000 INFO @ Fri, 26 Jun 2020 08:55:12: 4000000 INFO @ Fri, 26 Jun 2020 08:55:18: 13000000 INFO @ Fri, 26 Jun 2020 08:55:19: 8000000 INFO @ Fri, 26 Jun 2020 08:55:20: 5000000 INFO @ Fri, 26 Jun 2020 08:55:26: 14000000 INFO @ Fri, 26 Jun 2020 08:55:27: 9000000 INFO @ Fri, 26 Jun 2020 08:55:28: 6000000 INFO @ Fri, 26 Jun 2020 08:55:33: 15000000 INFO @ Fri, 26 Jun 2020 08:55:36: 7000000 INFO @ Fri, 26 Jun 2020 08:55:36: 10000000 INFO @ Fri, 26 Jun 2020 08:55:41: 16000000 INFO @ Fri, 26 Jun 2020 08:55:44: 8000000 INFO @ Fri, 26 Jun 2020 08:55:45: 11000000 INFO @ Fri, 26 Jun 2020 08:55:49: 17000000 INFO @ Fri, 26 Jun 2020 08:55:51: 9000000 INFO @ Fri, 26 Jun 2020 08:55:53: 12000000 INFO @ Fri, 26 Jun 2020 08:55:57: 18000000 INFO @ Fri, 26 Jun 2020 08:55:59: 10000000 INFO @ Fri, 26 Jun 2020 08:56:01: 13000000 INFO @ Fri, 26 Jun 2020 08:56:05: 19000000 INFO @ Fri, 26 Jun 2020 08:56:07: 11000000 INFO @ Fri, 26 Jun 2020 08:56:10: 14000000 INFO @ Fri, 26 Jun 2020 08:56:12: 20000000 INFO @ Fri, 26 Jun 2020 08:56:16: 12000000 INFO @ Fri, 26 Jun 2020 08:56:19: 15000000 INFO @ Fri, 26 Jun 2020 08:56:20: 21000000 INFO @ Fri, 26 Jun 2020 08:56:24: 13000000 INFO @ Fri, 26 Jun 2020 08:56:27: 16000000 INFO @ Fri, 26 Jun 2020 08:56:28: 22000000 INFO @ Fri, 26 Jun 2020 08:56:32: 14000000 INFO @ Fri, 26 Jun 2020 08:56:36: 17000000 INFO @ Fri, 26 Jun 2020 08:56:36: 23000000 INFO @ Fri, 26 Jun 2020 08:56:38: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 08:56:38: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 08:56:38: #1 total tags in treatment: 23196680 INFO @ Fri, 26 Jun 2020 08:56:38: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:56:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:56:38: #1 tags after filtering in treatment: 23196680 INFO @ Fri, 26 Jun 2020 08:56:38: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:56:38: #1 finished! INFO @ Fri, 26 Jun 2020 08:56:38: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:56:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:56:40: 15000000 INFO @ Fri, 26 Jun 2020 08:56:40: #2 number of paired peaks: 13 WARNING @ Fri, 26 Jun 2020 08:56:40: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:56:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:56:44: 18000000 INFO @ Fri, 26 Jun 2020 08:56:48: 16000000 INFO @ Fri, 26 Jun 2020 08:56:52: 19000000 INFO @ Fri, 26 Jun 2020 08:56:56: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 08:57:01: 20000000 INFO @ Fri, 26 Jun 2020 08:57:04: 18000000 INFO @ Fri, 26 Jun 2020 08:57:09: 21000000 INFO @ Fri, 26 Jun 2020 08:57:12: 19000000 INFO @ Fri, 26 Jun 2020 08:57:17: 22000000 INFO @ Fri, 26 Jun 2020 08:57:20: 20000000 INFO @ Fri, 26 Jun 2020 08:57:25: 23000000 INFO @ Fri, 26 Jun 2020 08:57:27: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 08:57:27: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 08:57:27: #1 total tags in treatment: 23196680 INFO @ Fri, 26 Jun 2020 08:57:27: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:57:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:57:27: #1 tags after filtering in treatment: 23196680 INFO @ Fri, 26 Jun 2020 08:57:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:57:27: #1 finished! INFO @ Fri, 26 Jun 2020 08:57:27: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:57:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:57:28: 21000000 INFO @ Fri, 26 Jun 2020 08:57:29: #2 number of paired peaks: 13 WARNING @ Fri, 26 Jun 2020 08:57:29: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:57:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:57:35: 22000000 INFO @ Fri, 26 Jun 2020 08:57:42: 23000000 INFO @ Fri, 26 Jun 2020 08:57:44: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 08:57:44: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 08:57:44: #1 total tags in treatment: 23196680 INFO @ Fri, 26 Jun 2020 08:57:44: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:57:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:57:44: #1 tags after filtering in treatment: 23196680 INFO @ Fri, 26 Jun 2020 08:57:44: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:57:44: #1 finished! INFO @ Fri, 26 Jun 2020 08:57:44: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:57:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:57:46: #2 number of paired peaks: 13 WARNING @ Fri, 26 Jun 2020 08:57:46: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:57:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495291/SRX495291.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。