Job ID = 6498427 SRX = SRX495284 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:24:01 prefetch.2.10.7: 1) Downloading 'SRR1198789'... 2020-06-25T23:24:01 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:29:39 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:29:39 prefetch.2.10.7: 1) 'SRR1198789' was downloaded successfully Read 50358398 spots for SRR1198789/SRR1198789.sra Written 50358398 spots for SRR1198789/SRR1198789.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:09 50358398 reads; of these: 50358398 (100.00%) were unpaired; of these: 961422 (1.91%) aligned 0 times 41800657 (83.01%) aligned exactly 1 time 7596319 (15.08%) aligned >1 times 98.09% overall alignment rate Time searching: 00:16:09 Overall time: 00:16:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 14891263 / 49396976 = 0.3015 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:58:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:58:38: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:58:38: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:58:43: 1000000 INFO @ Fri, 26 Jun 2020 08:58:48: 2000000 INFO @ Fri, 26 Jun 2020 08:58:53: 3000000 INFO @ Fri, 26 Jun 2020 08:58:58: 4000000 INFO @ Fri, 26 Jun 2020 08:59:03: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:59:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:59:08: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:59:08: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:59:08: 6000000 INFO @ Fri, 26 Jun 2020 08:59:13: 1000000 INFO @ Fri, 26 Jun 2020 08:59:13: 7000000 INFO @ Fri, 26 Jun 2020 08:59:18: 2000000 INFO @ Fri, 26 Jun 2020 08:59:18: 8000000 INFO @ Fri, 26 Jun 2020 08:59:24: 3000000 INFO @ Fri, 26 Jun 2020 08:59:24: 9000000 INFO @ Fri, 26 Jun 2020 08:59:29: 4000000 INFO @ Fri, 26 Jun 2020 08:59:29: 10000000 INFO @ Fri, 26 Jun 2020 08:59:34: 5000000 INFO @ Fri, 26 Jun 2020 08:59:34: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:59:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:59:38: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:59:38: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:59:40: 6000000 INFO @ Fri, 26 Jun 2020 08:59:40: 12000000 INFO @ Fri, 26 Jun 2020 08:59:43: 1000000 INFO @ Fri, 26 Jun 2020 08:59:45: 7000000 INFO @ Fri, 26 Jun 2020 08:59:45: 13000000 INFO @ Fri, 26 Jun 2020 08:59:49: 2000000 INFO @ Fri, 26 Jun 2020 08:59:51: 8000000 INFO @ Fri, 26 Jun 2020 08:59:51: 14000000 INFO @ Fri, 26 Jun 2020 08:59:54: 3000000 INFO @ Fri, 26 Jun 2020 08:59:56: 9000000 INFO @ Fri, 26 Jun 2020 08:59:56: 15000000 INFO @ Fri, 26 Jun 2020 09:00:00: 4000000 INFO @ Fri, 26 Jun 2020 09:00:01: 10000000 INFO @ Fri, 26 Jun 2020 09:00:02: 16000000 INFO @ Fri, 26 Jun 2020 09:00:05: 5000000 INFO @ Fri, 26 Jun 2020 09:00:07: 11000000 INFO @ Fri, 26 Jun 2020 09:00:07: 17000000 INFO @ Fri, 26 Jun 2020 09:00:10: 6000000 INFO @ Fri, 26 Jun 2020 09:00:12: 18000000 INFO @ Fri, 26 Jun 2020 09:00:12: 12000000 INFO @ Fri, 26 Jun 2020 09:00:16: 7000000 INFO @ Fri, 26 Jun 2020 09:00:18: 13000000 INFO @ Fri, 26 Jun 2020 09:00:18: 19000000 INFO @ Fri, 26 Jun 2020 09:00:21: 8000000 INFO @ Fri, 26 Jun 2020 09:00:23: 14000000 INFO @ Fri, 26 Jun 2020 09:00:23: 20000000 INFO @ Fri, 26 Jun 2020 09:00:27: 9000000 INFO @ Fri, 26 Jun 2020 09:00:28: 21000000 INFO @ Fri, 26 Jun 2020 09:00:28: 15000000 INFO @ Fri, 26 Jun 2020 09:00:32: 10000000 INFO @ Fri, 26 Jun 2020 09:00:34: 22000000 INFO @ Fri, 26 Jun 2020 09:00:34: 16000000 INFO @ Fri, 26 Jun 2020 09:00:37: 11000000 INFO @ Fri, 26 Jun 2020 09:00:39: 17000000 INFO @ Fri, 26 Jun 2020 09:00:39: 23000000 INFO @ Fri, 26 Jun 2020 09:00:43: 12000000 INFO @ Fri, 26 Jun 2020 09:00:44: 24000000 INFO @ Fri, 26 Jun 2020 09:00:44: 18000000 INFO @ Fri, 26 Jun 2020 09:00:48: 13000000 INFO @ Fri, 26 Jun 2020 09:00:50: 25000000 INFO @ Fri, 26 Jun 2020 09:00:50: 19000000 INFO @ Fri, 26 Jun 2020 09:00:54: 14000000 INFO @ Fri, 26 Jun 2020 09:00:55: 26000000 INFO @ Fri, 26 Jun 2020 09:00:55: 20000000 INFO @ Fri, 26 Jun 2020 09:00:59: 15000000 INFO @ Fri, 26 Jun 2020 09:01:01: 21000000 INFO @ Fri, 26 Jun 2020 09:01:01: 27000000 INFO @ Fri, 26 Jun 2020 09:01:05: 16000000 INFO @ Fri, 26 Jun 2020 09:01:06: 22000000 INFO @ Fri, 26 Jun 2020 09:01:06: 28000000 INFO @ Fri, 26 Jun 2020 09:01:11: 17000000 INFO @ Fri, 26 Jun 2020 09:01:12: 23000000 INFO @ Fri, 26 Jun 2020 09:01:13: 29000000 INFO @ Fri, 26 Jun 2020 09:01:16: 18000000 INFO @ Fri, 26 Jun 2020 09:01:18: 24000000 INFO @ Fri, 26 Jun 2020 09:01:18: 30000000 INFO @ Fri, 26 Jun 2020 09:01:22: 19000000 INFO @ Fri, 26 Jun 2020 09:01:23: 25000000 INFO @ Fri, 26 Jun 2020 09:01:24: 31000000 INFO @ Fri, 26 Jun 2020 09:01:28: 20000000 INFO @ Fri, 26 Jun 2020 09:01:29: 26000000 INFO @ Fri, 26 Jun 2020 09:01:30: 32000000 INFO @ Fri, 26 Jun 2020 09:01:34: 21000000 INFO @ Fri, 26 Jun 2020 09:01:35: 27000000 INFO @ Fri, 26 Jun 2020 09:01:36: 33000000 INFO @ Fri, 26 Jun 2020 09:01:40: 22000000 INFO @ Fri, 26 Jun 2020 09:01:41: 28000000 INFO @ Fri, 26 Jun 2020 09:01:42: 34000000 INFO @ Fri, 26 Jun 2020 09:01:45: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 09:01:45: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 09:01:45: #1 total tags in treatment: 34505713 INFO @ Fri, 26 Jun 2020 09:01:45: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:01:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:01:45: #1 tags after filtering in treatment: 34505713 INFO @ Fri, 26 Jun 2020 09:01:45: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:01:45: #1 finished! INFO @ Fri, 26 Jun 2020 09:01:45: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:01:45: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:01:46: 23000000 INFO @ Fri, 26 Jun 2020 09:01:47: 29000000 INFO @ Fri, 26 Jun 2020 09:01:48: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 09:01:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:01:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:01:51: 24000000 INFO @ Fri, 26 Jun 2020 09:01:52: 30000000 INFO @ Fri, 26 Jun 2020 09:01:57: 25000000 INFO @ Fri, 26 Jun 2020 09:01:58: 31000000 INFO @ Fri, 26 Jun 2020 09:02:03: 26000000 INFO @ Fri, 26 Jun 2020 09:02:04: 32000000 INFO @ Fri, 26 Jun 2020 09:02:09: 27000000 INFO @ Fri, 26 Jun 2020 09:02:09: 33000000 INFO @ Fri, 26 Jun 2020 09:02:15: 34000000 INFO @ Fri, 26 Jun 2020 09:02:15: 28000000 INFO @ Fri, 26 Jun 2020 09:02:18: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 09:02:18: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 09:02:18: #1 total tags in treatment: 34505713 INFO @ Fri, 26 Jun 2020 09:02:18: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:02:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:02:19: #1 tags after filtering in treatment: 34505713 INFO @ Fri, 26 Jun 2020 09:02:19: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:02:19: #1 finished! INFO @ Fri, 26 Jun 2020 09:02:19: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:02:19: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:02:21: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 09:02:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:02:21: Process for pairing-model is terminated! INFO @ Fri, 26 Jun 2020 09:02:21: 29000000 cut: /home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:02:27: 30000000 INFO @ Fri, 26 Jun 2020 09:02:32: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 09:02:38: 32000000 INFO @ Fri, 26 Jun 2020 09:02:43: 33000000 INFO @ Fri, 26 Jun 2020 09:02:49: 34000000 INFO @ Fri, 26 Jun 2020 09:02:52: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 09:02:52: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 09:02:52: #1 total tags in treatment: 34505713 INFO @ Fri, 26 Jun 2020 09:02:52: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:02:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:02:53: #1 tags after filtering in treatment: 34505713 INFO @ Fri, 26 Jun 2020 09:02:53: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:02:53: #1 finished! INFO @ Fri, 26 Jun 2020 09:02:53: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:02:53: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:02:55: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 09:02:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:02:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495284/SRX495284.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。