Job ID = 1305025 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 22,615,922 reads read : 22,615,922 reads written : 22,615,922 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:20 22615922 reads; of these: 22615922 (100.00%) were unpaired; of these: 2060549 (9.11%) aligned 0 times 17410292 (76.98%) aligned exactly 1 time 3145081 (13.91%) aligned >1 times 90.89% overall alignment rate Time searching: 00:06:20 Overall time: 00:06:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4552225 / 20555373 = 0.2215 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 21:16:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:16:14: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:16:14: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:16:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:16:14: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:16:14: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:16:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:16:14: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:16:14: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:16:25: 1000000 INFO @ Mon, 03 Jun 2019 21:16:26: 1000000 INFO @ Mon, 03 Jun 2019 21:16:26: 1000000 INFO @ Mon, 03 Jun 2019 21:16:34: 2000000 INFO @ Mon, 03 Jun 2019 21:16:37: 2000000 INFO @ Mon, 03 Jun 2019 21:16:38: 2000000 INFO @ Mon, 03 Jun 2019 21:16:43: 3000000 INFO @ Mon, 03 Jun 2019 21:16:48: 3000000 INFO @ Mon, 03 Jun 2019 21:16:48: 3000000 INFO @ Mon, 03 Jun 2019 21:16:52: 4000000 INFO @ Mon, 03 Jun 2019 21:16:59: 4000000 INFO @ Mon, 03 Jun 2019 21:16:59: 4000000 INFO @ Mon, 03 Jun 2019 21:17:01: 5000000 INFO @ Mon, 03 Jun 2019 21:17:10: 6000000 INFO @ Mon, 03 Jun 2019 21:17:10: 5000000 INFO @ Mon, 03 Jun 2019 21:17:10: 5000000 INFO @ Mon, 03 Jun 2019 21:17:19: 7000000 INFO @ Mon, 03 Jun 2019 21:17:21: 6000000 INFO @ Mon, 03 Jun 2019 21:17:21: 6000000 INFO @ Mon, 03 Jun 2019 21:17:28: 8000000 INFO @ Mon, 03 Jun 2019 21:17:33: 7000000 INFO @ Mon, 03 Jun 2019 21:17:33: 7000000 INFO @ Mon, 03 Jun 2019 21:17:37: 9000000 INFO @ Mon, 03 Jun 2019 21:17:44: 8000000 INFO @ Mon, 03 Jun 2019 21:17:44: 8000000 INFO @ Mon, 03 Jun 2019 21:17:46: 10000000 INFO @ Mon, 03 Jun 2019 21:17:56: 9000000 INFO @ Mon, 03 Jun 2019 21:17:56: 9000000 INFO @ Mon, 03 Jun 2019 21:17:57: 11000000 INFO @ Mon, 03 Jun 2019 21:18:08: 10000000 INFO @ Mon, 03 Jun 2019 21:18:08: 10000000 INFO @ Mon, 03 Jun 2019 21:18:10: 12000000 INFO @ Mon, 03 Jun 2019 21:18:20: 11000000 INFO @ Mon, 03 Jun 2019 21:18:21: 13000000 INFO @ Mon, 03 Jun 2019 21:18:21: 11000000 INFO @ Mon, 03 Jun 2019 21:18:32: 14000000 INFO @ Mon, 03 Jun 2019 21:18:33: 12000000 INFO @ Mon, 03 Jun 2019 21:18:35: 12000000 INFO @ Mon, 03 Jun 2019 21:18:43: 15000000 INFO @ Mon, 03 Jun 2019 21:18:44: 13000000 INFO @ Mon, 03 Jun 2019 21:18:48: 13000000 INFO @ Mon, 03 Jun 2019 21:18:53: 16000000 INFO @ Mon, 03 Jun 2019 21:18:54: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:18:54: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:18:54: #1 total tags in treatment: 16003148 INFO @ Mon, 03 Jun 2019 21:18:54: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:18:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:18:54: #1 tags after filtering in treatment: 16003148 INFO @ Mon, 03 Jun 2019 21:18:54: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:18:54: #1 finished! INFO @ Mon, 03 Jun 2019 21:18:54: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:18:54: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:18:55: #2 number of paired peaks: 52 WARNING @ Mon, 03 Jun 2019 21:18:55: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:18:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:18:56: 14000000 INFO @ Mon, 03 Jun 2019 21:19:01: 14000000 INFO @ Mon, 03 Jun 2019 21:19:08: 15000000 INFO @ Mon, 03 Jun 2019 21:19:12: 15000000 INFO @ Mon, 03 Jun 2019 21:19:18: 16000000 INFO @ Mon, 03 Jun 2019 21:19:18: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:19:18: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:19:18: #1 total tags in treatment: 16003148 INFO @ Mon, 03 Jun 2019 21:19:18: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:19:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:19:19: #1 tags after filtering in treatment: 16003148 INFO @ Mon, 03 Jun 2019 21:19:19: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:19:19: #1 finished! INFO @ Mon, 03 Jun 2019 21:19:19: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:19:19: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:19:20: #2 number of paired peaks: 52 WARNING @ Mon, 03 Jun 2019 21:19:20: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:19:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:19:23: 16000000 INFO @ Mon, 03 Jun 2019 21:19:23: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:19:23: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:19:23: #1 total tags in treatment: 16003148 INFO @ Mon, 03 Jun 2019 21:19:23: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:19:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:19:23: #1 tags after filtering in treatment: 16003148 INFO @ Mon, 03 Jun 2019 21:19:23: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:19:23: #1 finished! INFO @ Mon, 03 Jun 2019 21:19:23: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:19:23: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:19:25: #2 number of paired peaks: 52 WARNING @ Mon, 03 Jun 2019 21:19:25: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:19:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495262/SRX495262.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。