Job ID = 2590623 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 24,517,599 reads read : 24,517,599 reads written : 24,517,599 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:41 24517599 reads; of these: 24517599 (100.00%) were unpaired; of these: 475584 (1.94%) aligned 0 times 19182367 (78.24%) aligned exactly 1 time 4859648 (19.82%) aligned >1 times 98.06% overall alignment rate Time searching: 00:07:41 Overall time: 00:07:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5244227 / 24042015 = 0.2181 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 23:09:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:09:13: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:09:13: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:09:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:09:14: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:09:14: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:09:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:09:15: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:09:15: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:09:24: 1000000 INFO @ Mon, 12 Aug 2019 23:09:24: 1000000 INFO @ Mon, 12 Aug 2019 23:09:25: 1000000 INFO @ Mon, 12 Aug 2019 23:09:33: 2000000 INFO @ Mon, 12 Aug 2019 23:09:33: 2000000 INFO @ Mon, 12 Aug 2019 23:09:34: 2000000 INFO @ Mon, 12 Aug 2019 23:09:41: 3000000 INFO @ Mon, 12 Aug 2019 23:09:43: 3000000 INFO @ Mon, 12 Aug 2019 23:09:43: 3000000 INFO @ Mon, 12 Aug 2019 23:09:49: 4000000 INFO @ Mon, 12 Aug 2019 23:09:52: 4000000 INFO @ Mon, 12 Aug 2019 23:09:53: 4000000 INFO @ Mon, 12 Aug 2019 23:09:57: 5000000 INFO @ Mon, 12 Aug 2019 23:10:01: 5000000 INFO @ Mon, 12 Aug 2019 23:10:02: 5000000 INFO @ Mon, 12 Aug 2019 23:10:06: 6000000 INFO @ Mon, 12 Aug 2019 23:10:11: 6000000 INFO @ Mon, 12 Aug 2019 23:10:12: 6000000 INFO @ Mon, 12 Aug 2019 23:10:15: 7000000 INFO @ Mon, 12 Aug 2019 23:10:21: 7000000 INFO @ Mon, 12 Aug 2019 23:10:21: 7000000 INFO @ Mon, 12 Aug 2019 23:10:23: 8000000 INFO @ Mon, 12 Aug 2019 23:10:31: 8000000 INFO @ Mon, 12 Aug 2019 23:10:31: 8000000 INFO @ Mon, 12 Aug 2019 23:10:31: 9000000 INFO @ Mon, 12 Aug 2019 23:10:39: 10000000 INFO @ Mon, 12 Aug 2019 23:10:40: 9000000 INFO @ Mon, 12 Aug 2019 23:10:40: 9000000 INFO @ Mon, 12 Aug 2019 23:10:47: 11000000 INFO @ Mon, 12 Aug 2019 23:10:50: 10000000 INFO @ Mon, 12 Aug 2019 23:10:50: 10000000 INFO @ Mon, 12 Aug 2019 23:10:55: 12000000 INFO @ Mon, 12 Aug 2019 23:11:00: 11000000 INFO @ Mon, 12 Aug 2019 23:11:00: 11000000 INFO @ Mon, 12 Aug 2019 23:11:03: 13000000 INFO @ Mon, 12 Aug 2019 23:11:10: 12000000 INFO @ Mon, 12 Aug 2019 23:11:10: 12000000 INFO @ Mon, 12 Aug 2019 23:11:11: 14000000 INFO @ Mon, 12 Aug 2019 23:11:19: 13000000 INFO @ Mon, 12 Aug 2019 23:11:19: 13000000 INFO @ Mon, 12 Aug 2019 23:11:20: 15000000 INFO @ Mon, 12 Aug 2019 23:11:28: 16000000 INFO @ Mon, 12 Aug 2019 23:11:29: 14000000 INFO @ Mon, 12 Aug 2019 23:11:29: 14000000 INFO @ Mon, 12 Aug 2019 23:11:39: 15000000 INFO @ Mon, 12 Aug 2019 23:11:39: 17000000 INFO @ Mon, 12 Aug 2019 23:11:41: 15000000 INFO @ Mon, 12 Aug 2019 23:11:47: 18000000 INFO @ Mon, 12 Aug 2019 23:11:49: 16000000 INFO @ Mon, 12 Aug 2019 23:11:51: 16000000 INFO @ Mon, 12 Aug 2019 23:11:54: #1 tag size is determined as 44 bps INFO @ Mon, 12 Aug 2019 23:11:54: #1 tag size = 44 INFO @ Mon, 12 Aug 2019 23:11:54: #1 total tags in treatment: 18797788 INFO @ Mon, 12 Aug 2019 23:11:54: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:11:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:11:54: #1 tags after filtering in treatment: 18797788 INFO @ Mon, 12 Aug 2019 23:11:54: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:11:54: #1 finished! INFO @ Mon, 12 Aug 2019 23:11:54: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:11:54: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:11:56: #2 number of paired peaks: 3 WARNING @ Mon, 12 Aug 2019 23:11:56: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:11:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 23:11:58: 17000000 INFO @ Mon, 12 Aug 2019 23:12:01: 17000000 INFO @ Mon, 12 Aug 2019 23:12:08: 18000000 INFO @ Mon, 12 Aug 2019 23:12:10: 18000000 INFO @ Mon, 12 Aug 2019 23:12:15: #1 tag size is determined as 44 bps INFO @ Mon, 12 Aug 2019 23:12:15: #1 tag size = 44 INFO @ Mon, 12 Aug 2019 23:12:15: #1 total tags in treatment: 18797788 INFO @ Mon, 12 Aug 2019 23:12:15: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:12:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:12:15: #1 tags after filtering in treatment: 18797788 INFO @ Mon, 12 Aug 2019 23:12:15: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:12:15: #1 finished! INFO @ Mon, 12 Aug 2019 23:12:15: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:12:15: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:12:17: #2 number of paired peaks: 3 WARNING @ Mon, 12 Aug 2019 23:12:17: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:12:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 23:12:17: #1 tag size is determined as 44 bps INFO @ Mon, 12 Aug 2019 23:12:17: #1 tag size = 44 INFO @ Mon, 12 Aug 2019 23:12:17: #1 total tags in treatment: 18797788 INFO @ Mon, 12 Aug 2019 23:12:17: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:12:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:12:18: #1 tags after filtering in treatment: 18797788 INFO @ Mon, 12 Aug 2019 23:12:18: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:12:18: #1 finished! INFO @ Mon, 12 Aug 2019 23:12:18: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:12:18: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:12:19: #2 number of paired peaks: 3 WARNING @ Mon, 12 Aug 2019 23:12:19: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:12:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495255/SRX495255.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。