Job ID = 6498417 SRX = SRX495252 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:51:22 prefetch.2.10.7: 1) Downloading 'SRR1198757'... 2020-06-25T23:51:22 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:54:05 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:54:05 prefetch.2.10.7: 1) 'SRR1198757' was downloaded successfully Read 19875673 spots for SRR1198757/SRR1198757.sra Written 19875673 spots for SRR1198757/SRR1198757.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:22 19875673 reads; of these: 19875673 (100.00%) were unpaired; of these: 806323 (4.06%) aligned 0 times 15987092 (80.44%) aligned exactly 1 time 3082258 (15.51%) aligned >1 times 95.94% overall alignment rate Time searching: 00:07:22 Overall time: 00:07:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3389630 / 19069350 = 0.1778 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:07:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:07:57: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:07:57: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:08:03: 1000000 INFO @ Fri, 26 Jun 2020 09:08:09: 2000000 INFO @ Fri, 26 Jun 2020 09:08:15: 3000000 INFO @ Fri, 26 Jun 2020 09:08:22: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:08:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:08:26: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:08:26: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:08:28: 5000000 INFO @ Fri, 26 Jun 2020 09:08:33: 1000000 INFO @ Fri, 26 Jun 2020 09:08:34: 6000000 INFO @ Fri, 26 Jun 2020 09:08:40: 2000000 INFO @ Fri, 26 Jun 2020 09:08:41: 7000000 INFO @ Fri, 26 Jun 2020 09:08:46: 3000000 INFO @ Fri, 26 Jun 2020 09:08:47: 8000000 INFO @ Fri, 26 Jun 2020 09:08:52: 4000000 INFO @ Fri, 26 Jun 2020 09:08:54: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:08:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:08:56: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:08:56: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:08:59: 5000000 INFO @ Fri, 26 Jun 2020 09:09:00: 10000000 INFO @ Fri, 26 Jun 2020 09:09:03: 1000000 INFO @ Fri, 26 Jun 2020 09:09:06: 6000000 INFO @ Fri, 26 Jun 2020 09:09:07: 11000000 INFO @ Fri, 26 Jun 2020 09:09:11: 2000000 INFO @ Fri, 26 Jun 2020 09:09:12: 7000000 INFO @ Fri, 26 Jun 2020 09:09:14: 12000000 INFO @ Fri, 26 Jun 2020 09:09:18: 3000000 INFO @ Fri, 26 Jun 2020 09:09:18: 8000000 INFO @ Fri, 26 Jun 2020 09:09:21: 13000000 INFO @ Fri, 26 Jun 2020 09:09:25: 9000000 INFO @ Fri, 26 Jun 2020 09:09:25: 4000000 INFO @ Fri, 26 Jun 2020 09:09:28: 14000000 INFO @ Fri, 26 Jun 2020 09:09:32: 10000000 INFO @ Fri, 26 Jun 2020 09:09:33: 5000000 INFO @ Fri, 26 Jun 2020 09:09:35: 15000000 INFO @ Fri, 26 Jun 2020 09:09:38: 11000000 INFO @ Fri, 26 Jun 2020 09:09:39: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 09:09:39: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 09:09:39: #1 total tags in treatment: 15679720 INFO @ Fri, 26 Jun 2020 09:09:39: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:09:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:09:40: #1 tags after filtering in treatment: 15679720 INFO @ Fri, 26 Jun 2020 09:09:40: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:09:40: #1 finished! INFO @ Fri, 26 Jun 2020 09:09:40: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:09:40: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:09:40: 6000000 INFO @ Fri, 26 Jun 2020 09:09:41: #2 number of paired peaks: 48 WARNING @ Fri, 26 Jun 2020 09:09:41: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:09:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:09:45: 12000000 INFO @ Fri, 26 Jun 2020 09:09:47: 7000000 INFO @ Fri, 26 Jun 2020 09:09:52: 13000000 INFO @ Fri, 26 Jun 2020 09:09:54: 8000000 INFO @ Fri, 26 Jun 2020 09:09:59: 14000000 INFO @ Fri, 26 Jun 2020 09:10:02: 9000000 INFO @ Fri, 26 Jun 2020 09:10:06: 15000000 INFO @ Fri, 26 Jun 2020 09:10:09: 10000000 INFO @ Fri, 26 Jun 2020 09:10:10: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 09:10:10: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 09:10:10: #1 total tags in treatment: 15679720 INFO @ Fri, 26 Jun 2020 09:10:10: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:10:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:10:11: #1 tags after filtering in treatment: 15679720 INFO @ Fri, 26 Jun 2020 09:10:11: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:10:11: #1 finished! INFO @ Fri, 26 Jun 2020 09:10:11: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:10:11: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:10:12: #2 number of paired peaks: 48 WARNING @ Fri, 26 Jun 2020 09:10:12: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:10:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:10:16: 11000000 INFO @ Fri, 26 Jun 2020 09:10:23: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 09:10:30: 13000000 INFO @ Fri, 26 Jun 2020 09:10:37: 14000000 INFO @ Fri, 26 Jun 2020 09:10:44: 15000000 INFO @ Fri, 26 Jun 2020 09:10:49: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 09:10:49: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 09:10:49: #1 total tags in treatment: 15679720 INFO @ Fri, 26 Jun 2020 09:10:49: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:10:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:10:49: #1 tags after filtering in treatment: 15679720 INFO @ Fri, 26 Jun 2020 09:10:49: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:10:49: #1 finished! INFO @ Fri, 26 Jun 2020 09:10:49: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:10:49: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:10:50: #2 number of paired peaks: 48 WARNING @ Fri, 26 Jun 2020 09:10:50: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:10:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 5 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495252/SRX495252.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。