Job ID = 6498413 SRX = SRX495248 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:45:38 prefetch.2.10.7: 1) Downloading 'SRR1198753'... 2020-06-25T23:45:38 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:47:34 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:47:35 prefetch.2.10.7: 'SRR1198753' is valid 2020-06-25T23:47:35 prefetch.2.10.7: 1) 'SRR1198753' was downloaded successfully Read 15295295 spots for SRR1198753/SRR1198753.sra Written 15295295 spots for SRR1198753/SRR1198753.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:22 15295295 reads; of these: 15295295 (100.00%) were unpaired; of these: 5642652 (36.89%) aligned 0 times 7934545 (51.88%) aligned exactly 1 time 1718098 (11.23%) aligned >1 times 63.11% overall alignment rate Time searching: 00:03:22 Overall time: 00:03:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1412116 / 9652643 = 0.1463 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:54:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:54:41: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:54:41: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:54:48: 1000000 INFO @ Fri, 26 Jun 2020 08:54:55: 2000000 INFO @ Fri, 26 Jun 2020 08:55:02: 3000000 INFO @ Fri, 26 Jun 2020 08:55:09: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:55:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:55:11: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:55:11: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:55:16: 5000000 INFO @ Fri, 26 Jun 2020 08:55:19: 1000000 INFO @ Fri, 26 Jun 2020 08:55:24: 6000000 INFO @ Fri, 26 Jun 2020 08:55:26: 2000000 INFO @ Fri, 26 Jun 2020 08:55:32: 7000000 INFO @ Fri, 26 Jun 2020 08:55:34: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:55:39: 8000000 INFO @ Fri, 26 Jun 2020 08:55:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:55:41: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:55:41: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:55:41: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 08:55:41: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 08:55:41: #1 total tags in treatment: 8240527 INFO @ Fri, 26 Jun 2020 08:55:41: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:55:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:55:41: #1 tags after filtering in treatment: 8240527 INFO @ Fri, 26 Jun 2020 08:55:41: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:55:41: #1 finished! INFO @ Fri, 26 Jun 2020 08:55:41: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:55:41: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:55:42: #2 number of paired peaks: 64 WARNING @ Fri, 26 Jun 2020 08:55:42: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:55:42: Process for pairing-model is terminated! INFO @ Fri, 26 Jun 2020 08:55:42: 4000000 cut: /home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:55:49: 1000000 INFO @ Fri, 26 Jun 2020 08:55:50: 5000000 INFO @ Fri, 26 Jun 2020 08:55:57: 2000000 INFO @ Fri, 26 Jun 2020 08:55:58: 6000000 INFO @ Fri, 26 Jun 2020 08:56:05: 3000000 INFO @ Fri, 26 Jun 2020 08:56:06: 7000000 INFO @ Fri, 26 Jun 2020 08:56:13: 4000000 INFO @ Fri, 26 Jun 2020 08:56:13: 8000000 INFO @ Fri, 26 Jun 2020 08:56:15: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 08:56:15: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 08:56:15: #1 total tags in treatment: 8240527 INFO @ Fri, 26 Jun 2020 08:56:15: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:56:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:56:15: #1 tags after filtering in treatment: 8240527 INFO @ Fri, 26 Jun 2020 08:56:15: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:56:15: #1 finished! INFO @ Fri, 26 Jun 2020 08:56:15: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:56:15: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:56:16: #2 number of paired peaks: 64 WARNING @ Fri, 26 Jun 2020 08:56:16: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:56:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:56:20: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 08:56:28: 6000000 INFO @ Fri, 26 Jun 2020 08:56:35: 7000000 INFO @ Fri, 26 Jun 2020 08:56:42: 8000000 BigWig に変換しました。 INFO @ Fri, 26 Jun 2020 08:56:44: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 08:56:44: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 08:56:44: #1 total tags in treatment: 8240527 INFO @ Fri, 26 Jun 2020 08:56:44: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:56:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:56:44: #1 tags after filtering in treatment: 8240527 INFO @ Fri, 26 Jun 2020 08:56:44: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:56:44: #1 finished! INFO @ Fri, 26 Jun 2020 08:56:44: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:56:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:56:45: #2 number of paired peaks: 64 WARNING @ Fri, 26 Jun 2020 08:56:45: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:56:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495248/SRX495248.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling