Job ID = 6498411 SRX = SRX495246 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:39:16 prefetch.2.10.7: 1) Downloading 'SRR1198751'... 2020-06-25T23:39:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:44:00 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:44:00 prefetch.2.10.7: 1) 'SRR1198751' was downloaded successfully Read 30861048 spots for SRR1198751/SRR1198751.sra Written 30861048 spots for SRR1198751/SRR1198751.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:07 30861048 reads; of these: 30861048 (100.00%) were unpaired; of these: 3582064 (11.61%) aligned 0 times 22320375 (72.33%) aligned exactly 1 time 4958609 (16.07%) aligned >1 times 88.39% overall alignment rate Time searching: 00:08:07 Overall time: 00:08:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5592969 / 27278984 = 0.2050 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:00:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:00:05: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:00:05: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:00:11: 1000000 INFO @ Fri, 26 Jun 2020 09:00:17: 2000000 INFO @ Fri, 26 Jun 2020 09:00:23: 3000000 INFO @ Fri, 26 Jun 2020 09:00:29: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:00:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:00:35: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:00:35: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:00:35: 5000000 INFO @ Fri, 26 Jun 2020 09:00:42: 1000000 INFO @ Fri, 26 Jun 2020 09:00:42: 6000000 INFO @ Fri, 26 Jun 2020 09:00:49: 7000000 INFO @ Fri, 26 Jun 2020 09:00:50: 2000000 INFO @ Fri, 26 Jun 2020 09:00:57: 8000000 INFO @ Fri, 26 Jun 2020 09:00:57: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:01:04: 9000000 INFO @ Fri, 26 Jun 2020 09:01:05: 4000000 INFO @ Fri, 26 Jun 2020 09:01:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:01:05: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:01:05: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:01:11: 10000000 INFO @ Fri, 26 Jun 2020 09:01:13: 5000000 INFO @ Fri, 26 Jun 2020 09:01:13: 1000000 INFO @ Fri, 26 Jun 2020 09:01:19: 11000000 INFO @ Fri, 26 Jun 2020 09:01:20: 2000000 INFO @ Fri, 26 Jun 2020 09:01:21: 6000000 INFO @ Fri, 26 Jun 2020 09:01:27: 12000000 INFO @ Fri, 26 Jun 2020 09:01:28: 3000000 INFO @ Fri, 26 Jun 2020 09:01:29: 7000000 INFO @ Fri, 26 Jun 2020 09:01:35: 13000000 INFO @ Fri, 26 Jun 2020 09:01:35: 4000000 INFO @ Fri, 26 Jun 2020 09:01:37: 8000000 INFO @ Fri, 26 Jun 2020 09:01:42: 14000000 INFO @ Fri, 26 Jun 2020 09:01:43: 5000000 INFO @ Fri, 26 Jun 2020 09:01:45: 9000000 INFO @ Fri, 26 Jun 2020 09:01:50: 15000000 INFO @ Fri, 26 Jun 2020 09:01:50: 6000000 INFO @ Fri, 26 Jun 2020 09:01:53: 10000000 INFO @ Fri, 26 Jun 2020 09:01:57: 16000000 INFO @ Fri, 26 Jun 2020 09:01:58: 7000000 INFO @ Fri, 26 Jun 2020 09:02:01: 11000000 INFO @ Fri, 26 Jun 2020 09:02:05: 17000000 INFO @ Fri, 26 Jun 2020 09:02:05: 8000000 INFO @ Fri, 26 Jun 2020 09:02:09: 12000000 INFO @ Fri, 26 Jun 2020 09:02:12: 18000000 INFO @ Fri, 26 Jun 2020 09:02:13: 9000000 INFO @ Fri, 26 Jun 2020 09:02:17: 13000000 INFO @ Fri, 26 Jun 2020 09:02:20: 19000000 INFO @ Fri, 26 Jun 2020 09:02:20: 10000000 INFO @ Fri, 26 Jun 2020 09:02:25: 14000000 INFO @ Fri, 26 Jun 2020 09:02:27: 20000000 INFO @ Fri, 26 Jun 2020 09:02:28: 11000000 INFO @ Fri, 26 Jun 2020 09:02:33: 15000000 INFO @ Fri, 26 Jun 2020 09:02:34: 21000000 INFO @ Fri, 26 Jun 2020 09:02:36: 12000000 INFO @ Fri, 26 Jun 2020 09:02:39: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 09:02:39: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 09:02:39: #1 total tags in treatment: 21686015 INFO @ Fri, 26 Jun 2020 09:02:39: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:02:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:02:39: #1 tags after filtering in treatment: 21686015 INFO @ Fri, 26 Jun 2020 09:02:39: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:02:39: #1 finished! INFO @ Fri, 26 Jun 2020 09:02:39: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:02:39: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:02:41: #2 number of paired peaks: 7 WARNING @ Fri, 26 Jun 2020 09:02:41: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:02:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:02:41: 16000000 INFO @ Fri, 26 Jun 2020 09:02:43: 13000000 INFO @ Fri, 26 Jun 2020 09:02:49: 17000000 INFO @ Fri, 26 Jun 2020 09:02:51: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 09:02:57: 18000000 INFO @ Fri, 26 Jun 2020 09:02:58: 15000000 INFO @ Fri, 26 Jun 2020 09:03:04: 19000000 INFO @ Fri, 26 Jun 2020 09:03:06: 16000000 INFO @ Fri, 26 Jun 2020 09:03:12: 20000000 INFO @ Fri, 26 Jun 2020 09:03:13: 17000000 INFO @ Fri, 26 Jun 2020 09:03:19: 21000000 INFO @ Fri, 26 Jun 2020 09:03:21: 18000000 INFO @ Fri, 26 Jun 2020 09:03:24: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 09:03:24: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 09:03:24: #1 total tags in treatment: 21686015 INFO @ Fri, 26 Jun 2020 09:03:24: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:03:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:03:25: #1 tags after filtering in treatment: 21686015 INFO @ Fri, 26 Jun 2020 09:03:25: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:03:25: #1 finished! INFO @ Fri, 26 Jun 2020 09:03:25: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:03:25: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:03:26: #2 number of paired peaks: 7 WARNING @ Fri, 26 Jun 2020 09:03:26: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:03:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:03:28: 19000000 INFO @ Fri, 26 Jun 2020 09:03:35: 20000000 INFO @ Fri, 26 Jun 2020 09:03:42: 21000000 BigWig に変換しました。 INFO @ Fri, 26 Jun 2020 09:03:47: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 09:03:47: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 09:03:47: #1 total tags in treatment: 21686015 INFO @ Fri, 26 Jun 2020 09:03:47: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:03:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:03:47: #1 tags after filtering in treatment: 21686015 INFO @ Fri, 26 Jun 2020 09:03:47: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:03:47: #1 finished! INFO @ Fri, 26 Jun 2020 09:03:47: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:03:47: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:03:49: #2 number of paired peaks: 7 WARNING @ Fri, 26 Jun 2020 09:03:49: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:03:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495246/SRX495246.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling