Job ID = 6498405 SRX = SRX495240 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:35:01 prefetch.2.10.7: 1) Downloading 'SRR1198745'... 2020-06-25T23:35:01 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:37:07 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:37:07 prefetch.2.10.7: 1) 'SRR1198745' was downloaded successfully Read 17486457 spots for SRR1198745/SRR1198745.sra Written 17486457 spots for SRR1198745/SRR1198745.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:18 17486457 reads; of these: 17486457 (100.00%) were unpaired; of these: 1118405 (6.40%) aligned 0 times 13348403 (76.34%) aligned exactly 1 time 3019649 (17.27%) aligned >1 times 93.60% overall alignment rate Time searching: 00:04:18 Overall time: 00:04:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3251798 / 16368052 = 0.1987 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:46:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:46:10: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:46:10: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:46:16: 1000000 INFO @ Fri, 26 Jun 2020 08:46:22: 2000000 INFO @ Fri, 26 Jun 2020 08:46:28: 3000000 INFO @ Fri, 26 Jun 2020 08:46:34: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:46:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:46:40: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:46:40: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:46:41: 5000000 INFO @ Fri, 26 Jun 2020 08:46:46: 1000000 INFO @ Fri, 26 Jun 2020 08:46:47: 6000000 INFO @ Fri, 26 Jun 2020 08:46:53: 2000000 INFO @ Fri, 26 Jun 2020 08:46:53: 7000000 INFO @ Fri, 26 Jun 2020 08:46:59: 3000000 INFO @ Fri, 26 Jun 2020 08:47:00: 8000000 INFO @ Fri, 26 Jun 2020 08:47:06: 4000000 INFO @ Fri, 26 Jun 2020 08:47:06: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:47:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:47:12: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:47:12: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:47:12: 5000000 INFO @ Fri, 26 Jun 2020 08:47:13: 10000000 INFO @ Fri, 26 Jun 2020 08:47:19: 1000000 INFO @ Fri, 26 Jun 2020 08:47:19: 6000000 INFO @ Fri, 26 Jun 2020 08:47:19: 11000000 INFO @ Fri, 26 Jun 2020 08:47:25: 2000000 INFO @ Fri, 26 Jun 2020 08:47:25: 7000000 INFO @ Fri, 26 Jun 2020 08:47:26: 12000000 INFO @ Fri, 26 Jun 2020 08:47:32: 3000000 INFO @ Fri, 26 Jun 2020 08:47:32: 8000000 INFO @ Fri, 26 Jun 2020 08:47:33: 13000000 INFO @ Fri, 26 Jun 2020 08:47:33: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:47:33: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:47:33: #1 total tags in treatment: 13116254 INFO @ Fri, 26 Jun 2020 08:47:33: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:47:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:47:34: #1 tags after filtering in treatment: 13116254 INFO @ Fri, 26 Jun 2020 08:47:34: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:47:34: #1 finished! INFO @ Fri, 26 Jun 2020 08:47:34: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:47:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:47:34: #2 number of paired peaks: 56 WARNING @ Fri, 26 Jun 2020 08:47:34: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:47:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:47:38: 4000000 INFO @ Fri, 26 Jun 2020 08:47:38: 9000000 INFO @ Fri, 26 Jun 2020 08:47:45: 5000000 INFO @ Fri, 26 Jun 2020 08:47:45: 10000000 INFO @ Fri, 26 Jun 2020 08:47:51: 6000000 INFO @ Fri, 26 Jun 2020 08:47:52: 11000000 INFO @ Fri, 26 Jun 2020 08:47:58: 7000000 INFO @ Fri, 26 Jun 2020 08:47:58: 12000000 INFO @ Fri, 26 Jun 2020 08:48:04: 8000000 INFO @ Fri, 26 Jun 2020 08:48:05: 13000000 INFO @ Fri, 26 Jun 2020 08:48:05: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:48:05: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:48:05: #1 total tags in treatment: 13116254 INFO @ Fri, 26 Jun 2020 08:48:05: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:48:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:48:06: #1 tags after filtering in treatment: 13116254 INFO @ Fri, 26 Jun 2020 08:48:06: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:48:06: #1 finished! INFO @ Fri, 26 Jun 2020 08:48:06: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:48:06: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:48:06: #2 number of paired peaks: 56 WARNING @ Fri, 26 Jun 2020 08:48:06: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:48:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:48:10: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 08:48:16: 10000000 INFO @ Fri, 26 Jun 2020 08:48:22: 11000000 INFO @ Fri, 26 Jun 2020 08:48:28: 12000000 INFO @ Fri, 26 Jun 2020 08:48:33: 13000000 INFO @ Fri, 26 Jun 2020 08:48:34: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:48:34: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:48:34: #1 total tags in treatment: 13116254 INFO @ Fri, 26 Jun 2020 08:48:34: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:48:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:48:34: #1 tags after filtering in treatment: 13116254 INFO @ Fri, 26 Jun 2020 08:48:34: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:48:34: #1 finished! INFO @ Fri, 26 Jun 2020 08:48:34: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:48:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:48:35: #2 number of paired peaks: 56 WARNING @ Fri, 26 Jun 2020 08:48:35: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:48:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495240/SRX495240.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。