Job ID = 6498395 SRX = SRX495230 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:45:07 prefetch.2.10.7: 1) Downloading 'SRR1198735'... 2020-06-25T23:45:07 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:47:42 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:47:42 prefetch.2.10.7: 1) 'SRR1198735' was downloaded successfully Read 21723799 spots for SRR1198735/SRR1198735.sra Written 21723799 spots for SRR1198735/SRR1198735.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:55 21723799 reads; of these: 21723799 (100.00%) were unpaired; of these: 98917 (0.46%) aligned 0 times 19003243 (87.48%) aligned exactly 1 time 2621639 (12.07%) aligned >1 times 99.54% overall alignment rate Time searching: 00:04:55 Overall time: 00:04:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4159048 / 21624882 = 0.1923 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:58:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:58:48: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:58:48: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:58:55: 1000000 INFO @ Fri, 26 Jun 2020 08:59:02: 2000000 INFO @ Fri, 26 Jun 2020 08:59:08: 3000000 INFO @ Fri, 26 Jun 2020 08:59:15: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:59:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:59:18: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:59:18: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:59:22: 5000000 INFO @ Fri, 26 Jun 2020 08:59:25: 1000000 INFO @ Fri, 26 Jun 2020 08:59:30: 6000000 INFO @ Fri, 26 Jun 2020 08:59:33: 2000000 INFO @ Fri, 26 Jun 2020 08:59:37: 7000000 INFO @ Fri, 26 Jun 2020 08:59:41: 3000000 INFO @ Fri, 26 Jun 2020 08:59:45: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:59:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:59:48: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:59:48: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:59:48: 4000000 INFO @ Fri, 26 Jun 2020 08:59:52: 9000000 INFO @ Fri, 26 Jun 2020 08:59:55: 1000000 INFO @ Fri, 26 Jun 2020 08:59:56: 5000000 INFO @ Fri, 26 Jun 2020 09:00:00: 10000000 INFO @ Fri, 26 Jun 2020 09:00:03: 2000000 INFO @ Fri, 26 Jun 2020 09:00:04: 6000000 INFO @ Fri, 26 Jun 2020 09:00:07: 11000000 INFO @ Fri, 26 Jun 2020 09:00:11: 3000000 INFO @ Fri, 26 Jun 2020 09:00:11: 7000000 INFO @ Fri, 26 Jun 2020 09:00:15: 12000000 INFO @ Fri, 26 Jun 2020 09:00:19: 4000000 INFO @ Fri, 26 Jun 2020 09:00:19: 8000000 INFO @ Fri, 26 Jun 2020 09:00:23: 13000000 INFO @ Fri, 26 Jun 2020 09:00:27: 5000000 INFO @ Fri, 26 Jun 2020 09:00:27: 9000000 INFO @ Fri, 26 Jun 2020 09:00:30: 14000000 INFO @ Fri, 26 Jun 2020 09:00:35: 6000000 INFO @ Fri, 26 Jun 2020 09:00:35: 10000000 INFO @ Fri, 26 Jun 2020 09:00:38: 15000000 INFO @ Fri, 26 Jun 2020 09:00:42: 11000000 INFO @ Fri, 26 Jun 2020 09:00:42: 7000000 INFO @ Fri, 26 Jun 2020 09:00:46: 16000000 INFO @ Fri, 26 Jun 2020 09:00:50: 12000000 INFO @ Fri, 26 Jun 2020 09:00:50: 8000000 INFO @ Fri, 26 Jun 2020 09:00:53: 17000000 INFO @ Fri, 26 Jun 2020 09:00:57: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:00:57: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:00:57: #1 total tags in treatment: 17465834 INFO @ Fri, 26 Jun 2020 09:00:57: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:00:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:00:57: #1 tags after filtering in treatment: 17465834 INFO @ Fri, 26 Jun 2020 09:00:57: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:00:57: #1 finished! INFO @ Fri, 26 Jun 2020 09:00:57: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:00:57: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:00:58: 13000000 INFO @ Fri, 26 Jun 2020 09:00:58: 9000000 INFO @ Fri, 26 Jun 2020 09:00:58: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 09:00:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:00:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:01:05: 14000000 INFO @ Fri, 26 Jun 2020 09:01:06: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 09:01:13: 15000000 INFO @ Fri, 26 Jun 2020 09:01:14: 11000000 INFO @ Fri, 26 Jun 2020 09:01:21: 16000000 INFO @ Fri, 26 Jun 2020 09:01:22: 12000000 INFO @ Fri, 26 Jun 2020 09:01:28: 17000000 INFO @ Fri, 26 Jun 2020 09:01:29: 13000000 INFO @ Fri, 26 Jun 2020 09:01:32: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:01:32: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:01:32: #1 total tags in treatment: 17465834 INFO @ Fri, 26 Jun 2020 09:01:32: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:01:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:01:32: #1 tags after filtering in treatment: 17465834 INFO @ Fri, 26 Jun 2020 09:01:32: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:01:32: #1 finished! INFO @ Fri, 26 Jun 2020 09:01:32: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:01:32: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:01:33: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 09:01:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:01:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:01:37: 14000000 INFO @ Fri, 26 Jun 2020 09:01:44: 15000000 BigWig に変換しました。 INFO @ Fri, 26 Jun 2020 09:01:52: 16000000 INFO @ Fri, 26 Jun 2020 09:01:59: 17000000 INFO @ Fri, 26 Jun 2020 09:02:02: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:02:02: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:02:02: #1 total tags in treatment: 17465834 INFO @ Fri, 26 Jun 2020 09:02:02: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:02:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:02:03: #1 tags after filtering in treatment: 17465834 INFO @ Fri, 26 Jun 2020 09:02:03: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:02:03: #1 finished! INFO @ Fri, 26 Jun 2020 09:02:03: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:02:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:02:04: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 09:02:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:02:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495230/SRX495230.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling