Job ID = 6498391 SRX = SRX495226 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:24:31 prefetch.2.10.7: 1) Downloading 'SRR1198731'... 2020-06-25T23:24:31 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:27:32 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:27:32 prefetch.2.10.7: 1) 'SRR1198731' was downloaded successfully Read 25111745 spots for SRR1198731/SRR1198731.sra Written 25111745 spots for SRR1198731/SRR1198731.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:22 25111745 reads; of these: 25111745 (100.00%) were unpaired; of these: 421072 (1.68%) aligned 0 times 20783166 (82.76%) aligned exactly 1 time 3907507 (15.56%) aligned >1 times 98.32% overall alignment rate Time searching: 00:06:22 Overall time: 00:06:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5654801 / 24690673 = 0.2290 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:40:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:40:42: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:40:42: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:40:47: 1000000 INFO @ Fri, 26 Jun 2020 08:40:53: 2000000 INFO @ Fri, 26 Jun 2020 08:40:58: 3000000 INFO @ Fri, 26 Jun 2020 08:41:04: 4000000 INFO @ Fri, 26 Jun 2020 08:41:09: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:41:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:41:12: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:41:12: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:41:15: 6000000 INFO @ Fri, 26 Jun 2020 08:41:18: 1000000 INFO @ Fri, 26 Jun 2020 08:41:21: 7000000 INFO @ Fri, 26 Jun 2020 08:41:24: 2000000 INFO @ Fri, 26 Jun 2020 08:41:27: 8000000 INFO @ Fri, 26 Jun 2020 08:41:30: 3000000 INFO @ Fri, 26 Jun 2020 08:41:33: 9000000 INFO @ Fri, 26 Jun 2020 08:41:37: 4000000 INFO @ Fri, 26 Jun 2020 08:41:40: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:41:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:41:42: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:41:42: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:41:43: 5000000 INFO @ Fri, 26 Jun 2020 08:41:46: 11000000 INFO @ Fri, 26 Jun 2020 08:41:48: 1000000 INFO @ Fri, 26 Jun 2020 08:41:49: 6000000 INFO @ Fri, 26 Jun 2020 08:41:52: 12000000 INFO @ Fri, 26 Jun 2020 08:41:54: 2000000 INFO @ Fri, 26 Jun 2020 08:41:55: 7000000 INFO @ Fri, 26 Jun 2020 08:41:59: 13000000 INFO @ Fri, 26 Jun 2020 08:42:01: 3000000 INFO @ Fri, 26 Jun 2020 08:42:02: 8000000 INFO @ Fri, 26 Jun 2020 08:42:05: 14000000 INFO @ Fri, 26 Jun 2020 08:42:07: 4000000 INFO @ Fri, 26 Jun 2020 08:42:08: 9000000 INFO @ Fri, 26 Jun 2020 08:42:11: 15000000 INFO @ Fri, 26 Jun 2020 08:42:13: 5000000 INFO @ Fri, 26 Jun 2020 08:42:14: 10000000 INFO @ Fri, 26 Jun 2020 08:42:18: 16000000 INFO @ Fri, 26 Jun 2020 08:42:19: 6000000 INFO @ Fri, 26 Jun 2020 08:42:21: 11000000 INFO @ Fri, 26 Jun 2020 08:42:24: 17000000 INFO @ Fri, 26 Jun 2020 08:42:26: 7000000 INFO @ Fri, 26 Jun 2020 08:42:27: 12000000 INFO @ Fri, 26 Jun 2020 08:42:30: 18000000 INFO @ Fri, 26 Jun 2020 08:42:32: 8000000 INFO @ Fri, 26 Jun 2020 08:42:33: 13000000 INFO @ Fri, 26 Jun 2020 08:42:36: 19000000 INFO @ Fri, 26 Jun 2020 08:42:37: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:42:37: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:42:37: #1 total tags in treatment: 19035872 INFO @ Fri, 26 Jun 2020 08:42:37: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:42:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:42:37: #1 tags after filtering in treatment: 19035872 INFO @ Fri, 26 Jun 2020 08:42:37: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:42:37: #1 finished! INFO @ Fri, 26 Jun 2020 08:42:37: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:42:37: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:42:38: 9000000 INFO @ Fri, 26 Jun 2020 08:42:38: #2 number of paired peaks: 6 WARNING @ Fri, 26 Jun 2020 08:42:38: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:42:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:42:40: 14000000 INFO @ Fri, 26 Jun 2020 08:42:44: 10000000 INFO @ Fri, 26 Jun 2020 08:42:46: 15000000 INFO @ Fri, 26 Jun 2020 08:42:51: 11000000 INFO @ Fri, 26 Jun 2020 08:42:52: 16000000 INFO @ Fri, 26 Jun 2020 08:42:57: 12000000 INFO @ Fri, 26 Jun 2020 08:42:58: 17000000 INFO @ Fri, 26 Jun 2020 08:43:03: 13000000 INFO @ Fri, 26 Jun 2020 08:43:05: 18000000 INFO @ Fri, 26 Jun 2020 08:43:09: 14000000 INFO @ Fri, 26 Jun 2020 08:43:11: 19000000 INFO @ Fri, 26 Jun 2020 08:43:11: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:43:11: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:43:11: #1 total tags in treatment: 19035872 INFO @ Fri, 26 Jun 2020 08:43:11: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:43:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:43:11: #1 tags after filtering in treatment: 19035872 INFO @ Fri, 26 Jun 2020 08:43:11: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:43:11: #1 finished! INFO @ Fri, 26 Jun 2020 08:43:11: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:43:11: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:43:13: #2 number of paired peaks: 6 WARNING @ Fri, 26 Jun 2020 08:43:13: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:43:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:43:15: 15000000 INFO @ Fri, 26 Jun 2020 08:43:21: 16000000 INFO @ Fri, 26 Jun 2020 08:43:27: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 08:43:32: 18000000 INFO @ Fri, 26 Jun 2020 08:43:38: 19000000 INFO @ Fri, 26 Jun 2020 08:43:38: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:43:38: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:43:38: #1 total tags in treatment: 19035872 INFO @ Fri, 26 Jun 2020 08:43:38: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:43:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:43:39: #1 tags after filtering in treatment: 19035872 INFO @ Fri, 26 Jun 2020 08:43:39: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:43:39: #1 finished! INFO @ Fri, 26 Jun 2020 08:43:39: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:43:39: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:43:40: #2 number of paired peaks: 6 WARNING @ Fri, 26 Jun 2020 08:43:40: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:43:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495226/SRX495226.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。