Job ID = 6498387 SRX = SRX495222 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:11:54 prefetch.2.10.7: 1) Downloading 'SRR1198727'... 2020-06-25T23:11:54 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:14:11 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:14:11 prefetch.2.10.7: 1) 'SRR1198727' was downloaded successfully Read 18189117 spots for SRR1198727/SRR1198727.sra Written 18189117 spots for SRR1198727/SRR1198727.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:13 18189117 reads; of these: 18189117 (100.00%) were unpaired; of these: 703109 (3.87%) aligned 0 times 12502383 (68.74%) aligned exactly 1 time 4983625 (27.40%) aligned >1 times 96.13% overall alignment rate Time searching: 00:06:13 Overall time: 00:06:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3085384 / 17486008 = 0.1764 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:27:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:27:04: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:27:04: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:27:10: 1000000 INFO @ Fri, 26 Jun 2020 08:27:17: 2000000 INFO @ Fri, 26 Jun 2020 08:27:23: 3000000 INFO @ Fri, 26 Jun 2020 08:27:29: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:27:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:27:34: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:27:34: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:27:36: 5000000 INFO @ Fri, 26 Jun 2020 08:27:41: 1000000 INFO @ Fri, 26 Jun 2020 08:27:43: 6000000 INFO @ Fri, 26 Jun 2020 08:27:48: 2000000 INFO @ Fri, 26 Jun 2020 08:27:50: 7000000 INFO @ Fri, 26 Jun 2020 08:27:55: 3000000 INFO @ Fri, 26 Jun 2020 08:27:57: 8000000 INFO @ Fri, 26 Jun 2020 08:28:02: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:28:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:28:04: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:28:04: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:28:04: 9000000 INFO @ Fri, 26 Jun 2020 08:28:09: 5000000 INFO @ Fri, 26 Jun 2020 08:28:11: 1000000 INFO @ Fri, 26 Jun 2020 08:28:11: 10000000 INFO @ Fri, 26 Jun 2020 08:28:16: 6000000 INFO @ Fri, 26 Jun 2020 08:28:18: 2000000 INFO @ Fri, 26 Jun 2020 08:28:19: 11000000 INFO @ Fri, 26 Jun 2020 08:28:23: 7000000 INFO @ Fri, 26 Jun 2020 08:28:25: 3000000 INFO @ Fri, 26 Jun 2020 08:28:26: 12000000 INFO @ Fri, 26 Jun 2020 08:28:31: 8000000 INFO @ Fri, 26 Jun 2020 08:28:31: 4000000 INFO @ Fri, 26 Jun 2020 08:28:34: 13000000 INFO @ Fri, 26 Jun 2020 08:28:38: 9000000 INFO @ Fri, 26 Jun 2020 08:28:38: 5000000 INFO @ Fri, 26 Jun 2020 08:28:41: 14000000 INFO @ Fri, 26 Jun 2020 08:28:44: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:28:44: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:28:44: #1 total tags in treatment: 14400624 INFO @ Fri, 26 Jun 2020 08:28:44: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:28:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:28:45: #1 tags after filtering in treatment: 14400624 INFO @ Fri, 26 Jun 2020 08:28:45: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:28:45: #1 finished! INFO @ Fri, 26 Jun 2020 08:28:45: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:28:45: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:28:45: 6000000 INFO @ Fri, 26 Jun 2020 08:28:45: #2 number of paired peaks: 39 WARNING @ Fri, 26 Jun 2020 08:28:45: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:28:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:28:46: 10000000 INFO @ Fri, 26 Jun 2020 08:28:52: 7000000 INFO @ Fri, 26 Jun 2020 08:28:53: 11000000 INFO @ Fri, 26 Jun 2020 08:28:59: 8000000 INFO @ Fri, 26 Jun 2020 08:29:00: 12000000 INFO @ Fri, 26 Jun 2020 08:29:06: 9000000 INFO @ Fri, 26 Jun 2020 08:29:07: 13000000 INFO @ Fri, 26 Jun 2020 08:29:13: 10000000 INFO @ Fri, 26 Jun 2020 08:29:14: 14000000 INFO @ Fri, 26 Jun 2020 08:29:17: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:29:17: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:29:17: #1 total tags in treatment: 14400624 INFO @ Fri, 26 Jun 2020 08:29:17: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:29:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:29:18: #1 tags after filtering in treatment: 14400624 INFO @ Fri, 26 Jun 2020 08:29:18: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:29:18: #1 finished! INFO @ Fri, 26 Jun 2020 08:29:18: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:29:18: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:29:18: #2 number of paired peaks: 39 WARNING @ Fri, 26 Jun 2020 08:29:18: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:29:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:29:20: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 08:29:26: 12000000 INFO @ Fri, 26 Jun 2020 08:29:33: 13000000 INFO @ Fri, 26 Jun 2020 08:29:39: 14000000 INFO @ Fri, 26 Jun 2020 08:29:42: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 08:29:42: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 08:29:42: #1 total tags in treatment: 14400624 INFO @ Fri, 26 Jun 2020 08:29:42: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:29:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:29:42: #1 tags after filtering in treatment: 14400624 INFO @ Fri, 26 Jun 2020 08:29:42: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:29:42: #1 finished! INFO @ Fri, 26 Jun 2020 08:29:42: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:29:42: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:29:43: #2 number of paired peaks: 39 WARNING @ Fri, 26 Jun 2020 08:29:43: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:29:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495222/SRX495222.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。