Job ID = 6498376 SRX = SRX495211 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T00:06:38 prefetch.2.10.7: 1) Downloading 'SRR1198716'... 2020-06-26T00:06:38 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T00:09:02 prefetch.2.10.7: HTTPS download succeed 2020-06-26T00:09:02 prefetch.2.10.7: 1) 'SRR1198716' was downloaded successfully Read 22529054 spots for SRR1198716/SRR1198716.sra Written 22529054 spots for SRR1198716/SRR1198716.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:38 22529054 reads; of these: 22529054 (100.00%) were unpaired; of these: 4394978 (19.51%) aligned 0 times 15516541 (68.87%) aligned exactly 1 time 2617535 (11.62%) aligned >1 times 80.49% overall alignment rate Time searching: 00:05:38 Overall time: 00:05:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3992199 / 18134076 = 0.2201 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:21:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:21:10: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:21:10: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:21:17: 1000000 INFO @ Fri, 26 Jun 2020 09:21:23: 2000000 INFO @ Fri, 26 Jun 2020 09:21:30: 3000000 INFO @ Fri, 26 Jun 2020 09:21:36: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:21:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:21:40: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:21:40: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:21:43: 5000000 INFO @ Fri, 26 Jun 2020 09:21:48: 1000000 INFO @ Fri, 26 Jun 2020 09:21:51: 6000000 INFO @ Fri, 26 Jun 2020 09:21:57: 2000000 INFO @ Fri, 26 Jun 2020 09:21:58: 7000000 INFO @ Fri, 26 Jun 2020 09:22:05: 3000000 INFO @ Fri, 26 Jun 2020 09:22:06: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:22:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:22:10: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:22:10: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:22:14: 4000000 INFO @ Fri, 26 Jun 2020 09:22:14: 9000000 INFO @ Fri, 26 Jun 2020 09:22:17: 1000000 INFO @ Fri, 26 Jun 2020 09:22:21: 10000000 INFO @ Fri, 26 Jun 2020 09:22:22: 5000000 INFO @ Fri, 26 Jun 2020 09:22:25: 2000000 INFO @ Fri, 26 Jun 2020 09:22:29: 11000000 INFO @ Fri, 26 Jun 2020 09:22:30: 6000000 INFO @ Fri, 26 Jun 2020 09:22:32: 3000000 INFO @ Fri, 26 Jun 2020 09:22:37: 12000000 INFO @ Fri, 26 Jun 2020 09:22:38: 7000000 INFO @ Fri, 26 Jun 2020 09:22:40: 4000000 INFO @ Fri, 26 Jun 2020 09:22:45: 13000000 INFO @ Fri, 26 Jun 2020 09:22:46: 8000000 INFO @ Fri, 26 Jun 2020 09:22:48: 5000000 INFO @ Fri, 26 Jun 2020 09:22:53: 14000000 INFO @ Fri, 26 Jun 2020 09:22:55: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:22:55: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:22:55: #1 total tags in treatment: 14141877 INFO @ Fri, 26 Jun 2020 09:22:55: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:22:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:22:55: #1 tags after filtering in treatment: 14141877 INFO @ Fri, 26 Jun 2020 09:22:55: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:22:55: #1 finished! INFO @ Fri, 26 Jun 2020 09:22:55: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:22:55: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:22:55: 9000000 INFO @ Fri, 26 Jun 2020 09:22:55: 6000000 INFO @ Fri, 26 Jun 2020 09:22:56: #2 number of paired peaks: 31 WARNING @ Fri, 26 Jun 2020 09:22:56: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:22:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:23:03: 7000000 INFO @ Fri, 26 Jun 2020 09:23:04: 10000000 INFO @ Fri, 26 Jun 2020 09:23:10: 8000000 INFO @ Fri, 26 Jun 2020 09:23:13: 11000000 INFO @ Fri, 26 Jun 2020 09:23:18: 9000000 INFO @ Fri, 26 Jun 2020 09:23:22: 12000000 INFO @ Fri, 26 Jun 2020 09:23:25: 10000000 INFO @ Fri, 26 Jun 2020 09:23:30: 13000000 INFO @ Fri, 26 Jun 2020 09:23:33: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 09:23:39: 14000000 INFO @ Fri, 26 Jun 2020 09:23:40: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:23:40: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:23:40: #1 total tags in treatment: 14141877 INFO @ Fri, 26 Jun 2020 09:23:40: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:23:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:23:41: 12000000 INFO @ Fri, 26 Jun 2020 09:23:41: #1 tags after filtering in treatment: 14141877 INFO @ Fri, 26 Jun 2020 09:23:41: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:23:41: #1 finished! INFO @ Fri, 26 Jun 2020 09:23:41: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:23:41: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:23:42: #2 number of paired peaks: 31 WARNING @ Fri, 26 Jun 2020 09:23:42: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:23:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:23:48: 13000000 INFO @ Fri, 26 Jun 2020 09:23:55: 14000000 INFO @ Fri, 26 Jun 2020 09:23:56: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:23:56: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:23:56: #1 total tags in treatment: 14141877 INFO @ Fri, 26 Jun 2020 09:23:56: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:23:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:23:56: #1 tags after filtering in treatment: 14141877 INFO @ Fri, 26 Jun 2020 09:23:56: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:23:56: #1 finished! INFO @ Fri, 26 Jun 2020 09:23:56: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:23:56: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:23:57: #2 number of paired peaks: 31 WARNING @ Fri, 26 Jun 2020 09:23:57: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:23:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495211/SRX495211.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。