Job ID = 6528258 SRX = SRX495210 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:52:29 prefetch.2.10.7: 1) Downloading 'SRR1198715'... 2020-06-29T14:52:29 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:53:50 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:53:51 prefetch.2.10.7: 'SRR1198715' is valid 2020-06-29T14:53:51 prefetch.2.10.7: 1) 'SRR1198715' was downloaded successfully Read 17232507 spots for SRR1198715/SRR1198715.sra Written 17232507 spots for SRR1198715/SRR1198715.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:25 17232507 reads; of these: 17232507 (100.00%) were unpaired; of these: 207569 (1.20%) aligned 0 times 12448979 (72.24%) aligned exactly 1 time 4575959 (26.55%) aligned >1 times 98.80% overall alignment rate Time searching: 00:05:25 Overall time: 00:05:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3298716 / 17024938 = 0.1938 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:08:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:08:08: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:08:08: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:08:13: 1000000 INFO @ Tue, 30 Jun 2020 00:08:18: 2000000 INFO @ Tue, 30 Jun 2020 00:08:22: 3000000 INFO @ Tue, 30 Jun 2020 00:08:27: 4000000 INFO @ Tue, 30 Jun 2020 00:08:31: 5000000 INFO @ Tue, 30 Jun 2020 00:08:36: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:08:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:08:38: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:08:38: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:08:41: 7000000 INFO @ Tue, 30 Jun 2020 00:08:43: 1000000 INFO @ Tue, 30 Jun 2020 00:08:45: 8000000 INFO @ Tue, 30 Jun 2020 00:08:48: 2000000 INFO @ Tue, 30 Jun 2020 00:08:50: 9000000 INFO @ Tue, 30 Jun 2020 00:08:53: 3000000 INFO @ Tue, 30 Jun 2020 00:08:55: 10000000 INFO @ Tue, 30 Jun 2020 00:08:57: 4000000 INFO @ Tue, 30 Jun 2020 00:09:00: 11000000 INFO @ Tue, 30 Jun 2020 00:09:02: 5000000 INFO @ Tue, 30 Jun 2020 00:09:05: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:09:07: 6000000 INFO @ Tue, 30 Jun 2020 00:09:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:09:08: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:09:08: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:09:09: 13000000 INFO @ Tue, 30 Jun 2020 00:09:12: 7000000 INFO @ Tue, 30 Jun 2020 00:09:13: #1 tag size is determined as 44 bps INFO @ Tue, 30 Jun 2020 00:09:13: #1 tag size = 44 INFO @ Tue, 30 Jun 2020 00:09:13: #1 total tags in treatment: 13726222 INFO @ Tue, 30 Jun 2020 00:09:13: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:09:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:09:13: #1 tags after filtering in treatment: 13726222 INFO @ Tue, 30 Jun 2020 00:09:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:09:13: #1 finished! INFO @ Tue, 30 Jun 2020 00:09:13: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:09:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:09:14: 1000000 INFO @ Tue, 30 Jun 2020 00:09:14: #2 number of paired peaks: 55 WARNING @ Tue, 30 Jun 2020 00:09:14: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:09:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:09:17: 8000000 INFO @ Tue, 30 Jun 2020 00:09:18: 2000000 INFO @ Tue, 30 Jun 2020 00:09:22: 9000000 INFO @ Tue, 30 Jun 2020 00:09:23: 3000000 INFO @ Tue, 30 Jun 2020 00:09:27: 10000000 INFO @ Tue, 30 Jun 2020 00:09:28: 4000000 INFO @ Tue, 30 Jun 2020 00:09:31: 11000000 INFO @ Tue, 30 Jun 2020 00:09:33: 5000000 INFO @ Tue, 30 Jun 2020 00:09:36: 12000000 INFO @ Tue, 30 Jun 2020 00:09:38: 6000000 INFO @ Tue, 30 Jun 2020 00:09:41: 13000000 INFO @ Tue, 30 Jun 2020 00:09:43: 7000000 INFO @ Tue, 30 Jun 2020 00:09:45: #1 tag size is determined as 44 bps INFO @ Tue, 30 Jun 2020 00:09:45: #1 tag size = 44 INFO @ Tue, 30 Jun 2020 00:09:45: #1 total tags in treatment: 13726222 INFO @ Tue, 30 Jun 2020 00:09:45: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:09:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:09:45: #1 tags after filtering in treatment: 13726222 INFO @ Tue, 30 Jun 2020 00:09:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:09:45: #1 finished! INFO @ Tue, 30 Jun 2020 00:09:45: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:09:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:09:46: #2 number of paired peaks: 55 WARNING @ Tue, 30 Jun 2020 00:09:46: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:09:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:09:48: 8000000 INFO @ Tue, 30 Jun 2020 00:09:53: 9000000 INFO @ Tue, 30 Jun 2020 00:09:58: 10000000 INFO @ Tue, 30 Jun 2020 00:10:03: 11000000 INFO @ Tue, 30 Jun 2020 00:10:08: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:10:13: 13000000 INFO @ Tue, 30 Jun 2020 00:10:16: #1 tag size is determined as 44 bps INFO @ Tue, 30 Jun 2020 00:10:16: #1 tag size = 44 INFO @ Tue, 30 Jun 2020 00:10:16: #1 total tags in treatment: 13726222 INFO @ Tue, 30 Jun 2020 00:10:16: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:10:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:10:16: #1 tags after filtering in treatment: 13726222 INFO @ Tue, 30 Jun 2020 00:10:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:10:16: #1 finished! INFO @ Tue, 30 Jun 2020 00:10:16: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:10:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:10:17: #2 number of paired peaks: 55 WARNING @ Tue, 30 Jun 2020 00:10:17: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:10:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495210/SRX495210.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。