Job ID = 6528254 SRX = SRX495205 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:17:21 prefetch.2.10.7: 1) Downloading 'SRR1198710'... 2020-06-29T15:17:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:18:52 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:18:53 prefetch.2.10.7: 'SRR1198710' is valid 2020-06-29T15:18:53 prefetch.2.10.7: 1) 'SRR1198710' was downloaded successfully Read 17232507 spots for SRR1198710/SRR1198710.sra Written 17232507 spots for SRR1198710/SRR1198710.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:07 17232507 reads; of these: 17232507 (100.00%) were unpaired; of these: 207609 (1.20%) aligned 0 times 12449020 (72.24%) aligned exactly 1 time 4575878 (26.55%) aligned >1 times 98.80% overall alignment rate Time searching: 00:06:07 Overall time: 00:06:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3297749 / 17024898 = 0.1937 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:36:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:36:16: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:36:16: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:36:22: 1000000 INFO @ Tue, 30 Jun 2020 00:36:28: 2000000 INFO @ Tue, 30 Jun 2020 00:36:34: 3000000 INFO @ Tue, 30 Jun 2020 00:36:40: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:36:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:36:46: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:36:46: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:36:46: 5000000 INFO @ Tue, 30 Jun 2020 00:36:52: 1000000 INFO @ Tue, 30 Jun 2020 00:36:52: 6000000 INFO @ Tue, 30 Jun 2020 00:36:58: 7000000 INFO @ Tue, 30 Jun 2020 00:36:58: 2000000 INFO @ Tue, 30 Jun 2020 00:37:04: 8000000 INFO @ Tue, 30 Jun 2020 00:37:04: 3000000 INFO @ Tue, 30 Jun 2020 00:37:10: 9000000 INFO @ Tue, 30 Jun 2020 00:37:10: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:37:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:37:16: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:37:16: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:37:16: 10000000 INFO @ Tue, 30 Jun 2020 00:37:17: 5000000 INFO @ Tue, 30 Jun 2020 00:37:22: 11000000 INFO @ Tue, 30 Jun 2020 00:37:22: 1000000 INFO @ Tue, 30 Jun 2020 00:37:23: 6000000 INFO @ Tue, 30 Jun 2020 00:37:28: 12000000 INFO @ Tue, 30 Jun 2020 00:37:29: 2000000 INFO @ Tue, 30 Jun 2020 00:37:30: 7000000 INFO @ Tue, 30 Jun 2020 00:37:35: 13000000 INFO @ Tue, 30 Jun 2020 00:37:35: 3000000 INFO @ Tue, 30 Jun 2020 00:37:36: 8000000 INFO @ Tue, 30 Jun 2020 00:37:39: #1 tag size is determined as 44 bps INFO @ Tue, 30 Jun 2020 00:37:39: #1 tag size = 44 INFO @ Tue, 30 Jun 2020 00:37:39: #1 total tags in treatment: 13727149 INFO @ Tue, 30 Jun 2020 00:37:39: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:37:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:37:40: #1 tags after filtering in treatment: 13727149 INFO @ Tue, 30 Jun 2020 00:37:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:37:40: #1 finished! INFO @ Tue, 30 Jun 2020 00:37:40: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:37:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:37:40: #2 number of paired peaks: 54 WARNING @ Tue, 30 Jun 2020 00:37:40: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:37:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:37:41: 4000000 INFO @ Tue, 30 Jun 2020 00:37:42: 9000000 INFO @ Tue, 30 Jun 2020 00:37:47: 5000000 INFO @ Tue, 30 Jun 2020 00:37:49: 10000000 INFO @ Tue, 30 Jun 2020 00:37:53: 6000000 INFO @ Tue, 30 Jun 2020 00:37:56: 11000000 INFO @ Tue, 30 Jun 2020 00:37:59: 7000000 INFO @ Tue, 30 Jun 2020 00:38:02: 12000000 INFO @ Tue, 30 Jun 2020 00:38:05: 8000000 INFO @ Tue, 30 Jun 2020 00:38:08: 13000000 INFO @ Tue, 30 Jun 2020 00:38:10: 9000000 INFO @ Tue, 30 Jun 2020 00:38:13: #1 tag size is determined as 44 bps INFO @ Tue, 30 Jun 2020 00:38:13: #1 tag size = 44 INFO @ Tue, 30 Jun 2020 00:38:13: #1 total tags in treatment: 13727149 INFO @ Tue, 30 Jun 2020 00:38:13: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:38:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:38:13: #1 tags after filtering in treatment: 13727149 INFO @ Tue, 30 Jun 2020 00:38:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:38:13: #1 finished! INFO @ Tue, 30 Jun 2020 00:38:13: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:38:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:38:14: #2 number of paired peaks: 54 WARNING @ Tue, 30 Jun 2020 00:38:14: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:38:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:38:16: 10000000 INFO @ Tue, 30 Jun 2020 00:38:22: 11000000 INFO @ Tue, 30 Jun 2020 00:38:28: 12000000 INFO @ Tue, 30 Jun 2020 00:38:34: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:38:38: #1 tag size is determined as 44 bps INFO @ Tue, 30 Jun 2020 00:38:38: #1 tag size = 44 INFO @ Tue, 30 Jun 2020 00:38:38: #1 total tags in treatment: 13727149 INFO @ Tue, 30 Jun 2020 00:38:38: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:38:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:38:38: #1 tags after filtering in treatment: 13727149 INFO @ Tue, 30 Jun 2020 00:38:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:38:38: #1 finished! INFO @ Tue, 30 Jun 2020 00:38:38: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:38:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:38:39: #2 number of paired peaks: 54 WARNING @ Tue, 30 Jun 2020 00:38:39: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:38:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495205/SRX495205.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。